LOCUS       RR202787                8199 bp    DNA     circular  SYN 
DEFINITION  Nckap1l (Myc-DDK-tagged ORF) - Rat NCK associated protein 1 like (Nckap1l), (10 ug)
ACCESSION   NM_001108119
VERSION     NM_001108119.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4356..4447 
                     /label="Myc-DDK"  
     misc_feature    4477..5062 
                     /label="PolyA signal"  
     misc_feature    4534..4553 
                     /label="Restriction Enzyme"  
     misc_feature    5338..6010 
                     /label="ColE1"  
     misc_feature    6440..7234 
                     /label="Kan/Neo"  
     misc_feature    7269..7571 
                     /label="SV40 ori"  
     misc_feature    7657..8134 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4338..4344 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4338 
                     /label="Nckap1l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGACC TCTGCTTACC AGCATAAGTT AGCAGAAAAG CTCACGATCC 
     1081 TGAATGACCG GGGTCAAGGA GTTCTCATTC GCATGTACAA CATCAAGAAG ACCTGTTCAG 
     1141 ATCCAAAATC CAAGCCACCT TTCTTACTGG AAAAGTCCAT GGAATCATGT CTCAAGTACA 
     1201 TCAACAAGAA GTTTCCCAAC ATAGATGTCC GAAACAGCAC GCAACATTTA GGACCAGTGC 
     1261 ATCGTGAAAA AGCTGAGATA ATTAGATTTC TCACTAACTA CTACCAGTCA TTTGTGGATG 
     1321 TCATGGAATT TCGGGATCAT GTATATGAAC TTCTCAACAC CATCGATGCC TGCCAGTGTC 
     1381 ATTTTGATAT CAATCTCAAC TTCGACTTCA CTCGGAGCTA CCTGGACTTG ATTGTGACTT 
     1441 ACACCTCAGT CATTTTACTC CTGTCACGGA TCGAGGACCG CAGGCTACTC ATCGGCATGT 
     1501 ACAACTGTGC CCATGAGATG CTCCATGGGC ACGGCGACCC CAGTTTTCCT CGCCTGGGCC 
     1561 AGATGGTACT AGAATATGAC CATCCTCTGA AGAAGCTGAC AGAAGAATTT GGACCTCACA 
     1621 CAAAGGCTGT GAGTGGAGCC CTGCTCTCTC TGTACTTCCT CTTTGTTCGG AGGAACCAGG 
     1681 GAGCTGAGCA GTGGCGCAGT GCCCAGCTCC TAAGCCTCAT CAGCAGCCCC CCGGCCATGA 
     1741 TTAACCCTGC TAACTCAGAC ACAATGGCCT GCGAGTATCT GTCTGTGGAA GTGATGGAGC 
     1801 GATGGATCAT AATTGGGTTT CTTCTTTGTC ACGGGTGCCT CAACTCCAAC AGCCAGTGCC 
     1861 AGAAGCTGTG GAAGCTGTGT CTGGAGGGTT CCCTGTACAT CACCCTCATC CGGGAAGATG 
     1921 TGCTACAGGT GCACAAGGTC ACCGAAGACC TGTTCAGCAG TTTGAAAGGG TACAGTAAGC 
     1981 GAGTAGCAGA CATCAAGGAG AGCAAGGAAC ATGCCATTAC AAACAGTGGC CAGTTTCACT 
     2041 GTCAACGGAG GCAGTTTCTG CGGACAGCTG TGAAGGAGTT GGAGACTGTG TTAAATGATG 
     2101 AGCCAGGCCT GCTGGGTCCT AAGGCCCTTT TTGCTTTCAT GGCCCTGTCT TTCATTCGTG 
     2161 ATGAGGTCAC TTGGCTGGTT CGCCATACAG AAAATGTCAC CAAGACTAAG ACACCCGAAG 
     2221 ACTATGCTGA CTCCATCGCA GAGCTTCTCT TCCTGTTGGA GGAGATCAGG GCTCTGGTCC 
     2281 GAAGACACAT CAAAGTGATC CAGCAGTACC ATCTCCAGTA CCTGGCCAGA TTTGATGTCC 
     2341 TCGTGCTTAG TGACATCATT CAGAACCTGA CTGTGTGTCC CGAGGAGGAG TCTGTCATTA 
     2401 TGTCCTCCTT TGTTAGTACG CTATCCTCTC TGAATCTTAA GCAAGTTGAT AATGAAGAGA 
     2461 AATTTGACTT TTCTGGATTA AGGCTGGACT GGTTTCGCCT ACAGGCATAT ACCAGTGTGG 
     2521 CAAAGGCACC TTTGCACCTG CACGAGAACC CTGACCTGGC CAAGGTCATG AACCTCATCA 
     2581 TCTTTCACTC CCAGATGTTG GACTCAGTAG AGAAAATGCT GGTGGAGACC TCTGACCTTT 
     2641 CTACTTTCTG CTTTCACCTC CGTACCTTTG AGAAGATGTT TGCTACGACC CTGGAGGAAC 
     2701 CAACCATGCT TCGCTACACC ATCGCCTTCC CCCTGATTTG TGCTCATTTT GTCCACTGCA 
     2761 TTCATGAAAT GTGCCCAGAG GAGTACCCTC ACCTGAAGAA CCATGGTCTT CACCACTGCA 
     2821 ACTCCTTCCT GGAAGAGCTG GCCAAGCAGA CCAGCAACTG TGTGCTTGAG ATATGTGCTG 
     2881 AACAGCGGAA CCTGAACGAA CAGCTTCTAC CCAAACACTG TGCCACCACC ATCAGCAAAG 
     2941 CCAAGAACAG GAAGTCCATG AAGCAGAGGC AAGCTCCCAG GAAAGGAGAG CCTGAGAGGG 
     3001 ACAAGCCAGG AGCAGAGAGC CACCGAAAGA ACCGCAGCCT TGTCACGAAC ATGGACAAGC 
     3061 TACATTTAAA CTTGACTGAG TTGGCACTGG CAATGAATCA TGTGCACAGT TTCTCTGTGT 
     3121 TTGAACACAC CATCTTCCCA TCAGAGTATC TCAGCAGTCA CCTGGAGTCC AGGCTCAACA 
     3181 GAGCCATCGT GTCTTTGGCT GGCTACAATG CCACAACCCA GGAGATCCTT AGGCCTTCCG 
     3241 AGCTTTTGGC AGGAGTCAAG GCGTACACCA GTTTCATCCA GTCATTGGCA CAGTTCTTGG 
     3301 GCACAGATGC TTCCAGAATT GTTCGCAATG CCCTCCTGCA GCAGACGCAG CCGCTGGACT 
     3361 CATGTGGCGA GCAGACAGTC ACCACTCTCT ACACAAACTG GTACCTAGAA AGTCTACTCA 
     3421 GACAGGCGAG CAGTGGAACC ATCGTCCTCT CTCCAGCCAT GCAGGCCTTC ATCAGCCTTC 
     3481 CCCGAGATGG GGAGCAGAAC TTCAGTGCTG AGGAGTTCTC AGACATCTCT GAGATGCGAG 
     3541 CCTTGGCTGA AATTCTTGGC CCCTATGGCA TGAAGTTCCT GAGTGAAAAC CTGATGTGGC 
     3601 ACGTGACTTC TCAGATTGTG GAGCTGAAGA AACTTGTGGT GGAAAACATG GATATCCTGG 
     3661 TTCAGATCAG ATCCAACTTC ACCAAGCCAG AATTGATGGC ATCTCTGCTG CCCCAGCTGA 
     3721 CAGGGGCTGA AAATGTGCTA AAACGAATGA CCATCATTGG GGTGATTCTC AGTTTTAGGG 
     3781 CCATGGCCCA AGAGGGACTT CAGGAGGTCA CCCTGAGTGT CTTTGAGCTG GCGTGTGCTG 
     3841 CAGGGGTGAG CTGTGACATT GACCCGGCCT TGGTGGCTGC CATTGCCAAT CTGAAAGCTG 
     3901 ATAACTCATC CCCTGAAGAA GAGTACAAGG TGGCGTGCCT ACTTTTGATT TTCCTCGCTG 
     3961 TTTCCCTCCC ACTCCTTGCC ACTGACCCTT CTTCCTTCTT CAGCATTGAG AAAGATGGCT 
     4021 ACAACAACAA CATCCACTGT TTGACCAAAG CCATCATCCA GGTGTCTGCT GCCCTTTTCA 
     4081 CTCTGTACAA CAAGAACATT GAAACGCACC TCAAGGAGTT TCTGGTGGTG GCCTCTGTCA 
     4141 GCCTCTTGCA GCTGGGCCAG GAGACTGACA AGCTCAAAAC CAGAAATCGT GAATCCATTT 
     4201 CTCTGCTCAT GCGCTTGGTG GTGGAGGAGT CACCCTTCTT GACCCTGGAC ATGCTGGAGT 
     4261 CCTGTTTCCC TTACGTTCTA CTACGCAATG CCTATCGGGA GGTTTCCCGG GCCTTCTACC 
     4321 TGAACCGTCT GCCAGCCAGT TCCCACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4381 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5161 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5221 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5281 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5341 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5401 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5461 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5521 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5581 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5641 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5701 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5761 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5821 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5881 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5941 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6001 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6061 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6121 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6181 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6241 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6301 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6361 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6421 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6481 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6541 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6601 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6661 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6721 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6781 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6841 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6901 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6961 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7021 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7081 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7141 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7201 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7261 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7321 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7381 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7441 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7621 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7681 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7741 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7801 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7861 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7921 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7981 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8041 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8101 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8161 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//