LOCUS       RR202754                8583 bp    DNA     circular  SYN 
DEFINITION  Lrp6 (Myc-DDK-tagged ORF) - Rat low density lipoprotein receptor-related protein 6 (Lrp6), (10 ug)
ACCESSION   NM_001107892
VERSION     NM_001107892.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4740..4831 
                     /label="Myc-DDK"  
     misc_feature    4861..5446 
                     /label="PolyA signal"  
     misc_feature    4918..4937 
                     /label="Restriction Enzyme"  
     misc_feature    5722..6394 
                     /label="ColE1"  
     misc_feature    6824..7618 
                     /label="Kan/Neo"  
     misc_feature    7653..7955 
                     /label="SV40 ori"  
     misc_feature    8041..8518 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4722..4728 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4722 
                     /label="Lrp6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACTGGACA GACTGGGGAG AAGTGCCAAA GATAGAACGT GCTGGAATGG 
     1081 ATGGTTCAAG TCGCTTTGTT ATAATAAACA CTGAGATTTA CTGGCCAAAT GGACTGACTC 
     1141 TGGATTATGA GGAGCGGAAG CTTTATTGGG CAGATGCAAA ACTTAATTTC ATCCATAAAT 
     1201 CAAACCTGGA CGGAACAAAT CGACAGGCAG TGGTTAAAGG TTCCCTTCCA CATCCTTTTG 
     1261 CCTTGACGTT ATTTGAGGAC ACATTGTACT GGACTGACTG GAATACACAC TCTATTTTGG 
     1321 CTTGCAACAA GTATACTGGC GAGGGTCTGC GTGAAATTCA TTCTAACATC TTCTCTCCCA 
     1381 TGGATATACA TGCTTTCAGC CAACAGAGGC AGCCAAATGC TACAAATCCA TGTGGAATTG 
     1441 ATAATGGTGG TTGTTCCCAT TTGTGTTTGA TGTCTCCAGT CAAGCCTTTT TATCAGTGTG 
     1501 CTTGTCCAAC TGGGGTCAAG CTTCTGGAGA GTGGAAAAAC CTGCAAAGAT GGTGCCACTG 
     1561 AACTATTGCT GTTAGCCCGA CGGACAGACT TGAGGCGCAT CTCTTTGGAT ACACCAGATT 
     1621 TCACCGACAT TGTCCTACAG TTAGAAGACA TCCGACATGC CATTGCCATA GACTACGACC 
     1681 CCGTGGAAGG CTACGTCTAC TGGACGGACG ATGAAGTACG GGCCATCCGT CGCTCCTTCA 
     1741 TTGATGGATC TGGCAGTCAG TTTGTGGTCA CGGCCCAGAT TGCCCATCCT GATGGTATTG 
     1801 CTGTTGACTG GGTGGCAAGA AACCTGTACT GGACAGACAC TGGCACTGAT CGGATAGAAG 
     1861 TGACAAGGCT CAATGGGACC ATGAGGAAGA TCCTGATTTC AGAGGACTTA GAGGAGCCCC 
     1921 GGGCTATTGT GTTAGATCCC ATGGCTGGGT ACATGTACTG GACGGACTGG GGAGAAATCC 
     1981 CAAAAATCGA GCGAGCTGCT CTGGACGGAT CTGACCGAGT AGTTCTTGTT AACACTTCCC 
     2041 TTGGTTGGCC AAATGGCTTA GCCCTGGATT ATGATGAAGG CACAATATAC TGGGGAGATG 
     2101 CCAAAACAGA CAAAATTGAG GTTATGAATA CTGATGGCAC TGGGAGGCGA GTGCTGGTGG 
     2161 AAGACAAGAT CCCTCACATA TTTGGGTTTA CCTTGCTGGG CGACTATGTT TACTGGACTG 
     2221 ACTGGCAGAG GCGGAGCATC GAGAGAGTGC ACAAGCGGAG TGCAGAGAGG GAGGTCATAA 
     2281 TAGACCAGCT GCCAGACCTC ATGGGACTGA AGGCCACAAG CGTTCACAGA GTCATTGGTT 
     2341 CTAACCCCTG TGCTGAGGAG AATGGAGGAT GTAGCCATCT TTGCCTCTAC AGGCCTCAGG 
     2401 GACTTCGATG TGCCTGTCCC ATTGGCTTTG AGCTCATTAG TGACATGAAG ACATGCATTG 
     2461 TTCCTGAGGC TTTCCTTCTG TTCTCACGGA GAGCAGATAT AAGACGCATC TCTTTGGAAA 
     2521 CAAACAACAA CAATGTGGCC ATTCCTCTCA CGGGTGTCAA AGAAGCTTCT GCTTTGGATT 
     2581 TTGATGTCAC AGACAACAGA ATTTACTGGA CCGATATATC CCTGAAGACT ATTAGCAGAG 
     2641 CTTTTATGAA TGGCAGTGCA CTGGAACATG TGGTAGAGTT CGGCTTAGAT TACCCAGAAG 
     2701 GCATGGCAGT GGACTGGCTT GGGAAGAACT TATACTGGGC AGACACAGGA ACAAATCGCA 
     2761 TTGAGGTGTC AAAGTTGGAT GGACAGCACC GACAGGTTTT GGTATGGAAA GACCTTGACA 
     2821 GTCCCCGAGC TCTGGCATTG GATCCTGCTG AAGGATTTAT GTATTGGACT GAGTGGGGAG 
     2881 GGAAGCCTAA GATTGACAGA GCGGCTATGG ATGGAAGTGA ACGCACTACA TTAGTTCCAA 
     2941 ATGTGGGTCG AGCAAATGGC CTTACCATCG ACTATGCTAA GAGGCGGCTT TACTGGACAG 
     3001 ACCTGGACAC TAACTTAATA GAATCTTCAA ATATGCTCGG ACTCAACCGT GAAGTTATAG 
     3061 CAGATGACTT GCCTCATCCT TTTGGCTTAA CTCAGTACCA AGATTACATC TACTGGACAG 
     3121 ACTGGAGCCG ACGCAGCATT GAACGTGCCA ACAAAACCAG TGGCCAAAAC CGCACCATCA 
     3181 TCCAGGGCCA TTTGGATTAT GTGATGGACA TCCTAGTCTT TCACTCTTCC CGGCAGGCAG 
     3241 GGTGGAATGA ATGTGCCTCC AGCAACGGGC ACTGCTCCCA CCTTTGCTTG GCTGTGCCTG 
     3301 TTGGAGGTTT TGTGTGTGGA TGCCCTGCCC ACTACTCCCT GAATGCTGAC AACAGGACCT 
     3361 GTAGTGCTCC TACCACCTTC CTGCTCTTCA GTCAGAAGAG CGCCATCAAC CGCATGGTGA 
     3421 TTGATGAACA ACAGAGTCCT GACATCATCC TTCCTATCCA CAGCCTTCGG AACGTCCGGG 
     3481 CCATTGATTA TGACCCTTTG GACAAGCAGC TCTACTGGAT TGACTCTCGA CAAAACATGA 
     3541 TACGAAAGGC ACAAGAAGAT GGTGGCCAGG GTTTTACTGT AGTTGTAAGC TCGGTTCCAA 
     3601 ATCAGAACCT TGAAATACAG CCCTATGATC TCAGCATTGA TATTTATAGC CGTTACATCT 
     3661 ATTGGACCTG TGAAGCTACC AATGTCATTG ATGTGACGAG ATTAGATGGT CGATCAGTTG 
     3721 GAGTGGTCCT AAAAGGCGAG CAAGACAGAC CTCGAGCCAT TGTGGTAAAC CCTGAGAAAG 
     3781 GGTATATGTA TTTTACCAAT CTTCAGGAAA GATCTCCCAA AATTGAACGG GCTGCCTTGG 
     3841 ATGGAACAGA ACGGGAGGTT CTCTTTTTCA GTGGCCTGAG TAAGCCCGTC GCTTTGGCTC 
     3901 TCGACAGCAA GCTGAGCAGG CTCTTCTGGG CTGACTCCGA CCTCCGGCGA ATTGAGAGCA 
     3961 GCGACCTCTC AGGTGCCAAC AGGATCGTGC TAGAGGACTC TAATATATTG CAGCCTGTGG 
     4021 GTCTGACCGT GTTTGAGAAC TGGCTCTACT GGATTGATAA GCAGCAACAG ATGATTGAAA 
     4081 AAATTGACAT GACTGGTCGC GAAGGCAGAA CCAAGGTCCA GGCTCGGATT GCTCAGCTCA 
     4141 GTGACATCCA TGCAGTGAAG GAGCTGAACC TTCAGGAATA CAGACAGCAC CCGTGTGCCC 
     4201 AGGATAATGG TGGCTGTTCA CATATTTGCC TTGTAAAAGG AGATGGTACC ACAAGATGCT 
     4261 CCTGTCCCAT GCATTTAGTT CTGCTTCAGG ATGAGCTGTC CTGTGGAGAG CCTCCAACAT 
     4321 GTTCTCCTCA GCAGTTTACG TGTTTCACTG GGGACATTGA CTGCATCCCT GTGGCCTGGC 
     4381 GGTGTGATGG GTTTACTGAA TGTGAAGACC ACAGTGATGA GCTCAACTGT CCTGTGTGCT 
     4441 CGGAGTCTCA GTTCCAGTGT GCCAGCGGGC AGTGCATTGA CGGCGCCCTT CGATGCAATG 
     4501 GTGACGCGAA CTGCCAGGAT AAATCGGATG AGAAGAACTG TGAAGTGCTT TGTCTAATTG 
     4561 ATCAGTTCCG CTGTGCCAAT GGTCAGTGCG TTGGAAAACA CAAGAAATGC GACCACAATG 
     4621 TGGACTGCAG TGACAGATCT GATGAACTGG ACTGCTATTT TGAACCCACC ACCATCCTAC 
     4681 AGGCCGTACA GCTACCGGCA CTTTGCACCG CCCACTACAC CCTGCAGCAC ACGCGTACGC 
     4741 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4801 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4861 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4921 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4981 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5041 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5101 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5161 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5221 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5281 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5341 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5401 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5461 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5521 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5581 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5641 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5701 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5761 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5821 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5881 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5941 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6001 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6061 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6121 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6181 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6241 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6301 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6361 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6421 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6481 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6541 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6601 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6661 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6721 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6781 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6841 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6901 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6961 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7021 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7081 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7141 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7201 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7261 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7321 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7381 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7441 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7501 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7561 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7621 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7681 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7741 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7801 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7861 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7921 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7981 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8041 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8101 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8161 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8221 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8281 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8341 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8401 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8461 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8521 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8581 TTT
//