LOCUS       RR202745                8388 bp    DNA     circular  SYN 
DEFINITION  Atp2b4 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, plasma membrane 4 (Atp2b4), (10 ug)
ACCESSION   NM_001005871
VERSION     NM_001005871.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4545..4636 
                     /label="Myc-DDK"  
     misc_feature    4666..5251 
                     /label="PolyA signal"  
     misc_feature    4723..4742 
                     /label="Restriction Enzyme"  
     misc_feature    5527..6199 
                     /label="ColE1"  
     misc_feature    6629..7423 
                     /label="Kan/Neo"  
     misc_feature    7458..7760 
                     /label="SV40 ori"  
     misc_feature    7846..8323 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4527..4533 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4527 
                     /label="Atp2b4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAATCCA TCAGGACATA ACTTGCCAGC CAACTCGGTA GCTGAGAGCT 
     1081 ATGAAGGGGA GTTTGGCTGC ACCTTAATGG ACCTGAGGAA GCTCATGGAG CTTCGTGCAG 
     1141 CTGATGCAGT GACCCAGATC AGTGCCCACT ATGGAAGTGT ACAAGAAATC TGCGCAAGAC 
     1201 TGAAAACCTC CCCTGTAGAA GGTCTGTCTG GGAACCCTGC AGATTTGGAG AAGCGTAGAC 
     1261 TTGTTTTTGG GAAGAACATG ATACCTCCGA AAAAGCCCAA GACGTTCTTA GAATTAGTGT 
     1321 GGGAAGCCCT GCAGGATGTC ACACTCATCA TCCTGGAGAT CGCAGCCATC ATATCCCTGG 
     1381 TCCTGTCCTT CTACCGGCCT CCTGGCGGGG AAAATGAAAT CTGTGGCCAC ATTGTAAGTA 
     1441 ACCCAGAAGA AGATGAGGAA GGAGAAACTG GCTGGATTGA AGGGGCAGCC ATTCTTGCCT 
     1501 CGGTGATCAT TGTGGTATTC GTGACAGCCT TCAACGACTG GAGCAAAGAG AAGCAGTTCC 
     1561 GGGGGCTGCA GAGTCGCATT GAGCTTGAGC AAAAATTCTC CATTATCCGG AATGGTCAGC 
     1621 TTATACAACT CCCAGTGGCT GAGATTGTGG TGGGAGATAT TGCCCAGGTC AAATACGGTG 
     1681 ACCTGCTGCC TGCCGACGGA ATCCTAATCC AGGGAAATGA TCTGAAGATT GACGAGAGCT 
     1741 CTTTGACAGG AGAATCAGAT CATGTCAAGA AGACTCTGGA CAAAGACCCC ATGTTGCTCT 
     1801 CAGGGACTCA TGTGATGGAA GGCTCTGGAC GGATGGTGGT GACTGCTGTG GGAATCAATT 
     1861 CCCAGACTGG AATCATCTTC ACCCTCTTAG GGGCTAATGA GGAAGAAGAC GATGAGAAAA 
     1921 AGAAGAAAGG TAAAAAACAA GGAGTCTCTG AAAATCGCAA CAAAGCAAAA ACCCAGGATG 
     1981 GAGTGGCCTT GGAAATCCAG CCACTCAACA GCCAGGAGGG GCTCGATAGT GAGGAAAAGG 
     2041 AGAAGAAAGC AAGCAAGGGA CCCAAGAAGG AGAAGTCGGT CCTGCAGGGC AAGCTCACAC 
     2101 GCCTGGCTGT GCAGATCGGG AAGGCAGGTC TGATCATGTC TATCCTCACA GTCCTCATCC 
     2161 TGATTCTATA CTTCGTGGTT GACAATTTCG TGATACAGCG CCGGGCATGG CTTCCTGAGT 
     2221 GCACTCCTGT GTACATCCAG TATTTTGTCA AGTTCTTCAT CATCGGAGTC ACTGTACTGG 
     2281 TAGTGGCTGT GCCAGAGGGG CTGCCACTGG CTGTCACCAT CTCGTTGGCC TACTCTGTAA 
     2341 AGAAAATGAT GAAGGACAAC AACTTGGTCC GGCACTTGGA CGCCTGTGAG ACGATGGGCA 
     2401 ACGCCACAGC CATCTGCTCT GATAAGACGG GCACGCTGAC CATGAACCGC ATGACCGTGG 
     2461 TACAAGCTTA CATTGGGGGC ACTCACTACC GCCAGATCCC AAAACCTGAC GACCTCCCAC 
     2521 CCAACGTTCT GGACCTCATA GTCAACAGCA TCTGTATCAA CTCTGCTTAC ACTTCTAAGA 
     2581 TTCTGCCTCC AGAGAAAGAG GGAGGCCTCC CCCGGCAAGT AGGCAACAAG ACAGAGTGTG 
     2641 GACTGCTGGG CTTTGTCACA GACCTGAAGC AGGACTACCA AGCAGTTCGC AGTGAGATGC 
     2701 CCGAGGAGAA GCTCTTCAAG GTGTACACCT TCAACTCAGT GCGCAAGTCC ATGAGCACCG 
     2761 TCATCAGGAA GCCGGAAGGG GGCTTCCGCG TGTTCAGCAA GGGCGCCTCC GAGATAATGC 
     2821 TGCGCAAGTG TGATCGGATC CTGAACAAGG AAGGAGGAAT TGTACCATTC AAAACTAAGG 
     2881 ACCGAGACAA TATGGTACGC AACGTCATTG AGCCCATGGC CAGTGAAGGA CTCCGGACCA 
     2941 TCGGCATAGC TTACCGAGAT TTTGATGGTG AAGAGCCCTC GTGGGAAAAT GAGAATGAGA 
     3001 TCTTCACTGG GCTGGTCTGC ATTGCAGTGG TGGGCATTGA GGACCCTGTG CGCCCAGAGG 
     3061 TACCAGATGC CATTAACAAG TGCAAACGGG CTGGCATTAC TGTCAGGATG GTGACAGGTG 
     3121 ACAACGTCAA CACAGCCAGG GCCATCGCCA CCAAATGTGG CATCTTGACT CCTGGTGATG 
     3181 ACTTCCTGTG TCTGGAAGGG AAAGAATTCA ACAGACTCAT ACGCAATGAG AAGGGCGAGG 
     3241 TCGAACAAGA AAAGCTGGAC AAGGTCTGGC CCAGGCTTCG GGTACTGGCA CGATCTTCTC 
     3301 CCACAGACAA GCACACATTG GTGAAAGGTA TCATTGACAG CAATATTGGG GAACAGAGGC 
     3361 AGGTGGTGGC TGTCACCGGT GATGGAACCA ATGATGGACC GGCTCTAAAG AAAGCAGATG 
     3421 TCGGTTTTGC TATGGGCATT GCAGGCACAG ATGTGGCTAA GGAGGCATCA GACATCATCC 
     3481 TGACTGATGA CAACTTCACC AGCATTGTGA AGGCAGTGAT GTGGGGCCGG AACGTATACG 
     3541 ACAGCATTTC CAAGTTCCTG CAGTTCCAGC TCACTGTCAA CGTGGTGGCC GTGATTGTGG 
     3601 CTTTCTCTGG AGCCTGCATC ACTCAGGATT CCCCACTGAA AGCGGTGCAG ATGCTGTGGG 
     3661 TGAACCTGAT CATGGACACG TTTGCTTCTC TGGCCCTGGC CACAGAGCCC CCGACCGATT 
     3721 CCCTGCTGAG GCGTCGCCCC TATGGGCGAA ACAAGCCCCT GATCTCTCGC ACAATGATGA 
     3781 AGAACATCCT GGGCCACGCG GTGTATCAGC TCGGAATCGT CTTCCTCCTC GTCTTTGCTG 
     3841 GCGACAAACT GTTTGACATT GACAGTGGGA GGAAGGCGCC TCTCAATTCA CCACCCAGCC 
     3901 AGCACTACAC CATTGTTTTC AACACGTTTG TGCTGATGCA ACTCTTCAAC GAGATCAACT 
     3961 CTCGGAAGAT CCATGGCGAG AAGAATGTCT TTGCAGGTGT CTACCGCAAC ATCATTTTCT 
     4021 GCTCTGTTGT TTTAGGCACG TTCTTCTGCC AGATCTTGAT CGTGGAAGTG GGAGGTAAGC 
     4081 CCTTCAGCTG CACAAACCTC ACCATGGAGC AGTGGATGTG GTGTCTCTTT ATAGGGATCG 
     4141 GAGAACTTTT GTGGGGCCAG GTCATCTCTG CAATACCTAC CAAGTCTCTA AAGTTCCTGA 
     4201 AAGAGGCGGG CCATGGCAGT GACAAAGAGG AGATCAGCAA GGACGCTGAG GGGCTGGAAG 
     4261 AGATCGACCA TGCCGAGATG GAGCTTCGCC GAGGCCAGAT CCTCTGGGTC CGTGGCCTGA 
     4321 ACCGGATTCA GACTCAGATT GAAGTAATTA ACAAATTCCA GACGGGAGCC TCTTTTAAGG 
     4381 GAGTCCTAAG GCGACAGAAC TTGAGTCAAC AACTTGATGT AAAACTGGTT CCTAGCTCCT 
     4441 ATTCAGAAGC AGTTGCGTCA GTCAGAACCT CCCCCTCCAC CTCTTCTGCC GTTACACCTC 
     4501 CTCCTGTGGG CAATCAGAGT GGTCAAAGTA TTTCAACGCG TACGCGGCCG CTCGAGCAGA 
     4561 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4621 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4681 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4741 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4801 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4861 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4921 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4981 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5041 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5101 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5161 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5221 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5281 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5341 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5401 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5461 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5521 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5581 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5641 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5701 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5761 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5821 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5881 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5941 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6001 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6061 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6121 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6181 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6241 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6301 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6361 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6421 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6481 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6541 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6601 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6661 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6721 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6781 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6841 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6901 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6961 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7021 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7081 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7141 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7201 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7261 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7321 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7381 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7441 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7501 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7561 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7621 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7801 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7861 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7921 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7981 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8041 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8101 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8161 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8221 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8281 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8341 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//