LOCUS       RR202687                6627 bp    DNA     circular  SYN 
DEFINITION  Ubqln1 (Myc-DDK-tagged ORF) - Rat ubiquilin 1 (Ubqln1), (10 ug)
ACCESSION   NM_053747
VERSION     NM_053747.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2784..2875 
                     /label="Myc-DDK"  
     misc_feature    2905..3490 
                     /label="PolyA signal"  
     misc_feature    2962..2981 
                     /label="Restriction Enzyme"  
     misc_feature    3766..4438 
                     /label="ColE1"  
     misc_feature    4868..5662 
                     /label="Kan/Neo"  
     misc_feature    5697..5999 
                     /label="SV40 ori"  
     misc_feature    6085..6562 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2766..2772 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2766 
                     /label="Ubqln1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGAGC GCAGAGAGCG GCGGCCCACC GGGCGCTCAG GACAGTGCGG 
     1081 CCGACAGCGG CCCCGCAGAG CCCAAGATCA TGAAGGTCAC GGTGAAGACG CCTAAAGAGA 
     1141 AGGAAGAGTT CGCTGTGCCC GAGAACAGCT CGGTCCAGCA GTTCAAAGAG GAAATCTCAA 
     1201 AACGTTTCAA ATCTCATATT GACCAACTTG TGTTGATATT TGCTGGAAAA ATTTTAAAAG 
     1261 ATCAAGATAC TTTGAGTCAG CATGGGATTC ATGATGGACT TACGGTTCAC CTTGTCATCA 
     1321 AAACCCAAAA CAGGCCGCAA GATAATTCAG CTCAGCAAAC AAATACCACT GGAAACAGTG 
     1381 TGACCAGTTC ACCAGCTCCT GACAGTAACC CCACGTCTGG GCCTGCTGCT AACAGCTCCT 
     1441 TTGGTTTAGG TGGACTTGGA GGACTTGCAG GTCTTAGTAG CTTGGGTTTA AATACCACCA 
     1501 ACTTCTCTGA ACTACAGAGC CAAATGCAGC GGCAACTTTT GTCTAACCCT GAAATGATGG 
     1561 TCCAGATCAT GGAAAACCCC TTTGTGCAGA GCATGCTCTC AAACCCTGAC CTGATGCGGC 
     1621 AGCTGATCAT GGCCAACCCA CAGATGCAGC AGCTGATACA GAGAAACCCA GAGATCAGTC 
     1681 ACATGCTCAA TAACCCAGAC ATAATGCGAC AGACATTGGA ACTTGCCAGA AACCCAGCGA 
     1741 TGATGCAGGA AATGATGAGA AACCAGGACC GAGCCTTGAG CAACCTAGAA AGTATCCCTG 
     1801 GGGGCTACAA CGCCTTACGG CGCATGTACA CAGATATTCA GGAGCCCATG CTAAACGCTG 
     1861 CGCAAGAGCA GTTTGGTGGT AATCCATTTG CTTCCTTAGT GAGCAGTCCG TCCTCAGCAG 
     1921 AAGGGACTCA GCCTTCTCGA ACAGAAAATA GGGATCCGCT ACCTAACCCT TGGGCTCCAC 
     1981 AGACTCCCCA GAGCTCTCCA GCCTCTGGCA GCACTGGCAG CACCACTAAC ACAGTGAGCA 
     2041 CCTCTGCTGG CAATGCCACC AGTACCCCGG CGGGACAGGG CACCTCAGGG CCCAACTTGG 
     2101 TGCCTGGAGC AGGAGCTAGT ATGTTCAACA CGCCAGGGAT GCAAAGCTTG TTGCAGCAAA 
     2161 TAACTGAGAA CCCTCAGCTC ATGCAGAACA TGCTGTCTGC CCCCTACATG AGAAGCATGA 
     2221 TGCAGTCACT GAGCCAGAAC CCCGACCTTG CCGCACAGAT GATGCTGAAT AACCCCCTAT 
     2281 TTGCTGGGAA CCCTCAGCTT CAAGAGCAAA TGAGACAACA GCTCCCAACC TTCCTCCAAC 
     2341 AAATGCAGAA CCCCGACACA CTGTCAGCCA TGTCGAACCC TAGAGCCATG CAGGCGCTGC 
     2401 TGCAAATCCA GCAGGGCTTG CAGACGTTGG CCACAGAAGC TCCCGGCCTC ATCCCAGGGT 
     2461 TTACTCCTGG CTTGGCAGCG GGCAATTCTG GAGGCCCTGC AGGAACCACT GCACCTAGCA 
     2521 CTGCACCTGG TGAGGATACG AACCCCCAAG GGGGCGCTGC TGAGCCAGGC CACCAGCAGT 
     2581 TTATCCAGCA AATGCTGCAG GCCCTCGCCG GGGTGAACCC TCAGCTGCAG AGTCCAGAAG 
     2641 TCAGATTTCA ACAGCAACTG GAACAGCTCA GTGCAATGGG ATTCTTGAAC CGTGAAGCAA 
     2701 ACTTACAAGC ACTGATAGCA ACAGGGGGCG ACATCAATGC GGCCATTGAA AGGTTGCTGG 
     2761 GTTCCCAGCC GTCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     2821 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     2881 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     2941 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3001 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3061 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3121 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3181 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3241 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3301 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3361 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3421 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3481 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3541 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3601 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3661 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3721 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     3781 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     3841 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     3901 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     3961 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4021 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4081 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4141 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4201 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4261 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4321 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4381 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4441 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4501 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4561 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4621 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4681 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4741 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     4801 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     4861 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     4921 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     4981 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5041 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5101 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5161 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5221 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5281 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5341 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5401 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5461 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5521 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5581 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5641 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5701 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     5761 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     5821 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     5881 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     5941 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6001 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6061 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6121 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6181 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6241 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6301 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6361 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6421 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6481 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6541 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6601 ATTTAACAAA AATTTAACGC GAATTTT
//