LOCUS       RR202674                7938 bp    DNA     circular  SYN 
DEFINITION  Ide (Myc-DDK-tagged ORF) - Rat insulin degrading enzyme (Ide), (10 ug)
ACCESSION   NM_013159
VERSION     NM_013159.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4095..4186 
                     /label="Myc-DDK"  
     misc_feature    4216..4801 
                     /label="PolyA signal"  
     misc_feature    4273..4292 
                     /label="Restriction Enzyme"  
     misc_feature    5077..5749 
                     /label="ColE1"  
     misc_feature    6179..6973 
                     /label="Kan/Neo"  
     misc_feature    7008..7310 
                     /label="SV40 ori"  
     misc_feature    7396..7873 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4077..4083 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4077 
                     /label="Ide"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAACGGG CTCGTGTGGC TGCTGCACCC CGCGCTGCCC AGCACCTTGC 
     1081 ACTCCATCCT CGGCGCTCGC CCGCCTCCCG TGAAGCGACT GTGTGGATTC CCAAAACAAA 
     1141 TTTACAGCAC AATGAATAAT CCGGCCATCC AGAGAATAGA AGACCATATT GTCAAGTCTC 
     1201 CTGAAGACAA ACGGGAATAT CGTGGACTAG AACTGGCCAA TGGTATCAAA GTGCTTCTCA 
     1261 TCAGTGATCC CACCACGGAC AAGTCATCAG CAGCACTCGA CGTGCACATA GGGTCACTGT 
     1321 CAGACCCTCC AAATATTCCT GGCTTAAGCC ATTTTTGTGA GCATATGCTG TTTTTGGGAA 
     1381 CAAAGAAATA TCCTAAGGAA AATGAATACA GCCAGTTTCT CAGTGAACAT GCTGGGAGTT 
     1441 CAAATGCATT TACCAGCGGA GAACACACCA ATTATTATTT CGATGTTTCC CATGAACACT 
     1501 TGGAAGGAGC CCTGGACAGG TTTGCCCAGT TTTTCCTGTG CCCCTTGTTT GATGCAAGTT 
     1561 GTAAGGACAG AGAGGTGAAC GCTGTCGATT CAGAGCATGA AAAGAATGTG ATGAATGATG 
     1621 CCTGGAGACT CTTCCAGCTG GAAAAGGCTA CAGGGAATCC CAAGCACCCC TTCAGCAAAT 
     1681 TCGGGACAGG AAACAAATAT ACTCTAGAGA CTCGACCCAA CCAAGAAGGC ATCGATGTAA 
     1741 GGGAAGAACT CTTGAAATTT CACTCTACGT ATTATTCGTC CAATCTGATG GCGATTTGTG 
     1801 TTTTAGGTCG AGAATCTTTA GATGACCTGA CTAATCTGGT GGTGAAGTTA TTTTCTGAAG 
     1861 TAGAGAATAA AAATGTCCCT TTGCCTGAAT TTCCTGAGCA CCCTTTCCAA GAAGAACATC 
     1921 TTAAACAACT TTATAAAATA GTACCCATTA AGGATATTAG GAATCTTTAT GTGACATTTC 
     1981 CCATACCAGA CCTTCAACAG TACTACAAAT CCAATCCCGG TCATTATCTC GGTCATCTGA 
     2041 TCGGGCATGA AGGTCCTGGA AGCCTGTTGT CAGAGCTCAA GTCAAAGGGC TGGGTAAACA 
     2101 CCCTGGTTGG GGGACAGAAG GAAGGAGCCC GAGGTTTTAT GTTTTTTATC ATTAATGTGG 
     2161 ACTTAACTGA AGAAGGGTTA TTACATGTTG AAGATATAAT TTTGCACATG TTTCAATACA 
     2221 TTCAGAAGCT ACGTGCTGAA GGACCTCAAG AATGGGTTTT CCAAGAGTGC AAGGACTTGA 
     2281 ACGCTGTCGC TTTCAGGTTT AAAGATAAAG AGAGGCCACG AGGCTACACA TCTAAGATTG 
     2341 CAGGGAAATT GCACTATTAT CCCCTAAATG GAGTGCTCAC AGCTGAATAT TTACTGGAAG 
     2401 AATTTAGACC CGACTTGATA GACATGGTTC TTGATAAACT CAGACCAGAA AATGTCCGGG 
     2461 TGGCAATAGT TTCTAAATCA TTTGAAGGGA AAACTGACCG CACAGAGCAG TGGTATGGAA 
     2521 CCCAGTACAA GCAAGAAGCT ATCCCAGAGG ACGTCATTCA GAAATGGCAA AATGCTGACC 
     2581 TGAATGGGAA ATTTAAACTT CCAACAAAGA ATGAATTCAT TCCTACAAAT TTTGAGATTT 
     2641 TGGCATTAGA AAAAGATGCA ACACCATACC CTGCTCTTAT TAAGGATACA GCCATGAGTA 
     2701 AGCTGTGGTT CAAACAAGAT GATAAATTTT TCTTGCCGAA AGCTTGTCTC AACTTTGAAT 
     2761 TTTTCAGCCC GTTTGCTTAT GTGGACCCCT TGCACTGTAA CATGGCCTAT TTGTACCTTG 
     2821 AACTCCTCAA AGACTCACTC AACGAGTATG CATATGCAGC AGAGCTAGCA GGCCTGAGCT 
     2881 ATGATCTCCA AAACACCATC TATGGGATGT ATCTCTCAGT GAAAGGTTAC AATGACAAAC 
     2941 AGCCAATTTT GCTAAAGAAG ATCACCGAGA AAATGGCTAC TTTTGAGATT GATAAAAAAA 
     3001 GATTTGAAAT TATCAAAGAG GCGTACATGC GATCTCTTAA TAATTTCCGG GCTGAGCAGC 
     3061 CTCACCAGCA CGCCATGTAC TACCTCCGTC TGCTGATGAC TGAAGTGGCC TGGACCAAAG 
     3121 ATGAGTTAAA AGAAGCCCTC GATGATGTGA CCCTCCCCCG TCTTAAGGCC TTCATACCTC 
     3181 AGCTGCTGTC CCGGCTGCAT ATTGAAGCCC TTCTCCATGG CAACATAACA AAGCAGGCTG 
     3241 CCTTAGGAGT TATGCAGATG GTAGAAGACA CCCTTATTGA GCATGCTCAC ACCAAACCTC 
     3301 TCCTTCCAAG TCAGCTAGTC CGGTACAGAG AAGTTCAGCT CCCCGACCGA GGATGGTTTG 
     3361 TTTACCAGCG GAGGAACGAA GTCCACAATA ACTGTGGCAT TGAGATTTAC TACCAGACAG 
     3421 ACATGCAGAG CACCTCGGAG AACATGTTCC TGGAGCTCTT CTGCCAGATT ATCTCTGAGC 
     3481 CTTGCTTCAA CACTCTGCGT ACCAAGGAGC AGCTTGGCTA TATTGTCTTC AGTGGACCTC 
     3541 GTCGGGCCAA CGGCATCCAG GGCTTGCGAT TCATCATCCA GTCAGAAAAA CCACCTCACT 
     3601 ACCTGGAAAG CAGAGTGGAA GCCTTCTTGA TCACCATGGA AAAGGCCATA GAGGACATGA 
     3661 CAGAGGAGGC TTTCCAAAAA CACATTCAGG CGTTAGCGAT TCGCCGACTC GACAAACCAA 
     3721 AGAAACTCTC TGCAGAGTGC GCGAAGTACT GGGGGGAGAT CATCTCCCAG CAGTACAATT 
     3781 ATGACAGAGA TAACATAGAG GTTGCATATT TAAAGACACT CAGCAAGGAT GATATCATCA 
     3841 AATTCTACAA GGAAATGTTG GCTGTGGACG CACCAAGGAG ACATAAAGTA TCCGTCCACG 
     3901 TTCTTGCCAG GGAAATGGAT TCTTGTCCTG TGGTTGGAGA GTTCCCCTCT CAGAATGATA 
     3961 TAAACCTTTC CGAAGCGCCA CCCTTGCCAC AACCTGAGGT GATTCATAAC ATGACTGAAT 
     4021 TCAAGCGCGG CCTGCCGCTG TTCCCCCTTG TGAAGCCACA CATTAACTTC ATGGCGGCAA 
     4081 AACTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4141 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4201 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4261 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4321 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4381 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4441 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4501 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4561 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4621 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4681 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4741 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4801 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4861 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4921 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4981 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5581 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5641 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5701 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5761 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5821 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5881 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5941 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6001 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6061 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6121 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6181 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6241 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6301 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6361 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6421 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6481 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6541 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6601 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6661 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6721 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6781 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6841 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6901 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6961 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7021 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7081 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7141 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7201 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7261 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7381 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7441 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7501 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7561 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7621 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7681 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7741 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7801 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7861 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7921 AAATTTAACG CGAATTTT
//