LOCUS       RR202655                8286 bp    DNA     circular  SYN 
DEFINITION  Polr1b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide B (Polr1b), (10 ug)
ACCESSION   NM_031773
VERSION     NM_031773.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Polr1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTCGAC AGCCGGTGGC GGAACCTGCC CAGCGGGCCC AGCCTAAAGC 
     1081 ATTTAACCGA CCCCTCGTAC GCGGTTCCTC CGGAGCAGCA AAAGGCGGCG TTGCAGGACC 
     1141 TGACGCGGGC GCACGTGGAC TCCTTCAACT ACGCAGTGCT GGAGGGGCTG AGCCACGCGG 
     1201 TGCAGGCCAT ACCTCCCTTC GAATTTGCTT TCAAAGATGA GCGCATATCT CTTACTATTG 
     1261 TGGACGCTGC CATCAGTCCA CCGGCAGTGC CCAAAGGGAC CATCTGCAAA GAGCTCAACA 
     1321 TTTATCCAGC TGAGTGCCGT GGCCGGAGGA GCACGTACCG AGGGAAGCTG ACGGCTGATA 
     1381 TCAGCTGGGC CGTGAATGGA GTCCCCAAAG GCATCATTAA ACAATTTCTT GGCWATGTTC 
     1441 CCATCATGGT GAAGTCCAAG CTTTGCAACT TATACAACCT TCCTCCTCAA GTCCTCATTG 
     1501 AGCACCACGA GGAGGCAGAG GAAATGGGCG GTTATTTTAT AATCAACGGC ATTGAGAAAG 
     1561 TCATCCGCAT GTTGATTATG CCTCGGAGAA ATTTTCCCAT CGCAATGATA AGACCGAAAT 
     1621 GGAAAAGCAG AGGGCTCGGC TACACTCAGT TCGGGGTTTC CATTCACTGT GTGAGAGAGG 
     1681 AGCACTCTGC TGTCAATATG AACCTTCACT ATGTGGAGAA CGGCACGGTC ATGTTAAACT 
     1741 TTATTTACCG CAAAGAGCTG TTTTTCCTTC CTTTGGGATT TGCACTTAAG GCACTTGTGA 
     1801 GCTTTTCTGA CTATCAGATT TTCCAGGAGC TCATCAAAGG CAAAGAGGAG GACTCTTTCT 
     1861 TTAAGAATTC TGTTTCTCAG ATGCTGAGGA TTGTAATGGA GGAGGGTTGT CACACACAGA 
     1921 AGCAGGTCCT CGACTATCTG GGCGAACGCT TCAGAGTAAA GCTCAGTCTT CCCGATTGGT 
     1981 ACCCTAATGC GGAAGCTGCC GAGTTCCTGT TTAACCAGTG TATCTGCATC CACTTGAAAT 
     2041 CCAACACTGA CAAGTTTTAC CTGCTCTGTC TCATGACCCG GAAGCTCTTT GCTTTAGCCA 
     2101 GAGGAGAGTG CATGGAGGAC AATCCTGACA GTTTAGTGAA TCAAGAAGTC CTTACCCCTG 
     2161 GGCAGCTCTT CCTGATGTTT CTGAAGGAAA AGATGGAGAA TTGGCTACTG TCTATTAAAA 
     2221 TAGCTTTAGA TAAAAGGGCT CAGAAGACCA ATGTTTCCAT AAACAATGAA AATTTGATGA 
     2281 AGATTTTTAG TATGGGAACA GAGCTAACAA GACCATTTGA ATATCTTCTT GCTACTGGAA 
     2341 ATCTGCGTTC TAAAACAGGT CTTGGCTTCA TGCAGGATTC TGGCCTGTGT GTTGTGGCTG 
     2401 ACAAGCTGAA CTTCATTCGC TATCTCTCCC ATTTCCGCTG TGTGCACAGA GGGGCTGACT 
     2461 TTGCCAAGAT GAGGACCACC ACCGTGCGCA AGCTGCTGCC AGAATCCTGG GGCTTCCTCT 
     2521 GCCCTGTGCA CACCCCAGAC GGGGCACCGT GTGGGCTGCT GAACCACCTG ACTGCTGTGT 
     2581 GTGAGGTTGT TACCAAGTTT GTGTACACAG CATCTATTCC AGCCTTGCTC TGTGGCTTAG 
     2641 GAGTCACTCC TGTTGATGCA GCACCATGTC GACCGTATAG TGACTGCTAC CCTGTCCTGC 
     2701 TGGATGGCGT CATGGTGGGC TGGGTGGATA AGGAGCTGGC TCCTGAAGTG GCAGACACTC 
     2761 TCCGTCGATT TAAGGTGTTG AGAGAAAGGA GATGTTCCTC CCTGGATGGA GGTGGCCCTG 
     2821 ATTCCCATGA CAGGAAAGCC AAGCCTGTAC CCAGGGCTGT TCCTCTTCAC CACTCCCTGC 
     2881 AGGCTGGTGA GGCCTGTGCA GAACCTGGAG CTGGGCAAAG AAGAGCTCGT TGGAACTATG 
     2941 GAGCAGCTCT TCATGAACAT TGCCATCTTC GAGGACGAGG TTTTTGGTGG AGTTTCCACA 
     3001 CACCAGGAGC TCTTCCCTCA CAGCCTGCTG AGGTGATCGC CAACTTCATC CCCTTCTCTG 
     3061 ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG ACCATGGGCT 
     3121 TCCCGCTGCT CACCTACCAA GACCGATCAG ATAATAAACT CTATCGTCTC CAGACACCCC 
     3181 AGAGCCCTCT AGTGAGACCG TGCATGTATG ATCATTATGA CATGGACAAC TATCCCATCG 
     3241 GGACAAACGC CATTGTGGCT GTGATCTCCT ACACTGGCTA TGATATGGAG GACGCCATGA 
     3301 TTGTAAACAA GGCCTCCTGG GAACGAGGCT TTGCTCATGG AAGTGTCTAC AAGTCTGAGT 
     3361 TCATAGACCT CTCTGAGAAA TTTAAGCAAG GGGATGATAG TCTGGTATTT GGGGTCAAAC 
     3421 CTGGTGACCC ACGGGTTATG CAGAAGCTGG ACAATGATGG CTTGCCATTC ATAGGAGCAA 
     3481 AGCTGGAGTT TGGTGATCCT TACTACGGCT ACCTAAACCT TAACACCGGA GAAGGCTTCG 
     3541 TGGTTTACTA TAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG TGCAGTAATG 
     3601 ACACAGGAAG TGGGAAGTTC AAGTGCGTCT GCGTCACCGT CCGAGTCCCC CGGAACCCAA 
     3661 CTATTGGAGA TAAGTTTGCC AGCCGTCACG GACAGAAGGG CATTTTGAGC AGATTGTGGC 
     3721 CAGCTGAGGA CATGCCTTTC ACAGAGAGTG GGATGATGCC GGACATTCTG TTTAATCCTC 
     3781 ATGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGTATGGCT GGGAAGTCAG 
     3841 CAGCTTTGCA TGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG GAGAACTCTG 
     3901 CCCTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCCGGCTA CAACTTCTAT GGCACGGAGA 
     3961 GATTGTACAG CGGCATCAGC GGGATGGAGC TGGAGGCTGA CATTTTCATT GGTGTGGTTT 
     4021 ATTACCAGCG CCTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA ACTGGAGCCA 
     4081 GGGACAAAGT CACCAACCAG CCCATTGGAG GCAGGAACGT CCAGGGTGGG ATCCGATTTG 
     4141 GGGAGATGGA GCGGGATGCT CTGTTGGCGC ACGGCACATC TTTCCTTCTG CATGACCGCC 
     4201 TCTTCAACTG CTCCGACCGC TCTGTGGCCC ACGTATGCGT GAAGTGTGGC AGTTTGCTTT 
     4261 CTCCGCTGCT CGAGAAGCCT CCCCCATCTT GGTCTGCGAT GCGTAACAGA AAATACAACT 
     4321 GCACCGTCTG CGGCCGCAGT GACTCCATCG ACACTGTCTC TGTGCCGTAT GTTTTCCGGT 
     4381 ACTTTGTAGC TGAGCTGGCT GCCATGAACA TCAAAGTGAA ACTGGACGTC ATTACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//