LOCUS       RR202632                8646 bp    DNA     circular  SYN 
DEFINITION  Magi1 (Myc-DDK-tagged ORF) - Rat membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), (10 ug)
ACCESSION   NM_001030045
VERSION     NM_001030045.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4803..4894 
                     /label="Myc-DDK"  
     misc_feature    4924..5509 
                     /label="PolyA signal"  
     misc_feature    4981..5000 
                     /label="Restriction Enzyme"  
     misc_feature    5785..6457 
                     /label="ColE1"  
     misc_feature    6887..7681 
                     /label="Kan/Neo"  
     misc_feature    7716..8018 
                     /label="SV40 ori"  
     misc_feature    8104..8581 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4785..4791 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4785 
                     /label="Magi1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAAGTG ATCCAGAAGA AGAACCACTG GACTGGCCGC GTTCACGAAT 
     1081 GCACCGTGAA GCGGGGACCC CAGGGCGAGC TGGGGGTGAC GGTGCTGGGG GGCGCGGAGC 
     1141 ATGGGGAGTT TCCGTACGTG GGGGCGGTGG CGGCGGCCGA GGCGGCGGGG CTTCCCGGCG 
     1201 GTGGCGAGGG GCCGAAGCTG GCTGAAGGTG AGCTGCTGCT GGAGGTGCAG GGGGTCCGGG 
     1261 TGTCCGGCTT GCCCCGCTAT GACGTGCTGG GAGTCATCGA CAGCTGCAAG GAGGCCGTCA 
     1321 CCTTCAAAGC CGTCAGACAA GGAGGAAGGC TCAACAAGGA CCTACGACAT TTCCTCAACC 
     1381 AACGGTTCCA GAAGGGGTCT CCAGATCATG AGCTCCAGCA GACCATAAGG GACAACCTCT 
     1441 ACCGCCATGC TGTGCCTTGC ACAACCCGGT CTCCCAGAGA AGGAGAAGTG CCTGGCGTCG 
     1501 ATTACAGCTT TCTGACTGTG AAGGAGTTCC TGGACCTCGA GCAGAGTGGG ACCCTGTTGG 
     1561 AAGTCGGCAC CTATGAAGGA AACTATTATG GGACACCCAA ACCTCCCAGC CAGCCAGTCA 
     1621 GTGGGAAAGT GATCACGACG GATGCCTTGC ACAGCCTGCA GTCTGGCTCC AAACAGTCGA 
     1681 CCCCTAAGCG AACAAAATCC TACAATGATA TGCAAAATGC TGGCATAGTC CACACGGAGA 
     1741 ATGAGGAAGA GGAGGATGTT CCTGAAATGA ACAGTAGCTT CACAGCCGAC TCTGGAGACC 
     1801 AGGACGAGCC CACTCTCCAA GAAGCGACGC TCCCGCCTGT GAATAGTAGC GCCCTTGCTG 
     1861 CTCCCATCAC GGACCCTTCT CAGAAGTTCC CTCAGTACCT ACCTCTTTCT GCAGAGGATA 
     1921 ATTTAGGTCC TCTACCTGAA AACTGGGAGA TGGCCTATAC TGAAAATGGA GAAGTCTATT 
     1981 TTATAGACCA CAACGCGAAA ACAACATCAT GGTTAGACCC TCGGTGCCTG AACAAACAGC 
     2041 AGAAGCCTCT GGAAGAATGT GAAGATGATG AAGGGGTACA CACCGAGGAG CTGGACAGTG 
     2101 AACTAGAGCT GCCTGCTGGC TGGGAAAAGA TTGAAGACCC TGTCTACGGT GTCTACTATG 
     2161 TAGACCACAT CAACAGGAAG ACGCAATATG AAAACCCAGT CCTAGAAGCC AAAAGGAAGA 
     2221 GACAGCTTGA ACAACAGCAG CAGCAGCAGC AGCACCAGCA GCAGCCGCAG CAGCCTCAGC 
     2281 CACCGCAGCC AGAAGAGTGG ACAGAAGATC ATGCATCTGT TGTGCCTCCT GTTGCTCCTT 
     2341 CCCATCCCCC AAGCAACCCG GAGCCAGCCA GGGAAGCTCC ACTTCAGGGC AAACCTTTTT 
     2401 TTACAAGAAA CCCTTCTGAG CTGGAAGGCA AGTTCATTCA CACAAAGCTA CGGAAAAGCA 
     2461 GTCGAGGCTT TGGCTTCACG GTGGTTGGAG GGGACGAACC TGATGAGTTC CTGCAGATCA 
     2521 AGAGCCTTGT CCTAGATGGT CCTGCTGCAC TGGATGGCAA GATGGAGACA GGAGATGTAA 
     2581 TCGTCAGTGT GAATGACACC TGTGTTTTGG GACACACACA TGCTCAAGTT GTGAAAATTT 
     2641 TCCAGTCCAT TCCCATTGGT GCCAGTGTGG ACCTTGAACT CTGCAGAGGT TATCCATTGC 
     2701 CTTTTGACCC GGATGACCCT AACACAAGTT TAGTGACCTC GGTGGCCATT TTGGACAAAG 
     2761 AACCAATAAT TGTAAACGGA CAAGAGACCT ATGATTCACC AGCAAGCCAC AGTAGTAAAA 
     2821 CGGGCAAAGT CAGCAACATG AAGGATGCCA GGCCAAGCAG CCCCGCAGAC GTGGCTTCCA 
     2881 ACAGTTCTCA TGGGTATCCC AACGACACAG TCTCCTTGGC TTCCTCCATA GCCACCCAGC 
     2941 CAGAGCTAAT AACCGTTCAC ATAGTCAAAG GGCCAATGGG ATTTGGCTTT ACGATCGCAG 
     3001 ATAGTCCCGG TGGGGGTGGC CAAAGAGTGA AGCAGATTGT TGACAGTCCG CGCTGCAGAG 
     3061 GCCTCAAAGA AGGGGATCTG ATTGTGGAGG TGAATAAGAG GAACGTGCAG GCCCTGACGC 
     3121 ACAATCAAGT CGTGGATATG CTGATTGAGT GTCCAAAGGG AAGTGAGGTC ACACTGTTGG 
     3181 TGCAGCGAGG AGGGCTACCA GTTCCCAAGA AGAGTCCAAA GTCGCAGCCA CTGGAGAGGA 
     3241 AAGACAGCCA GAACAGCTCC CAGCACAGTG TGTCCAGCCT CCGGAGCCTG CACACAGCTT 
     3301 CCCCGAGCCA CAGTGCACAG GTGCTCCCTG AGTACCCACC TGCAGACGTC CCTGCTCCAG 
     3361 ATCAGACCGA CAGCTCTGGG CAGAAAAAGC CAGATCCCTT TAAAATCTGG GCCCAGTCGA 
     3421 GGAGCATGTA TGAAGACCGA CCTATGTCAC CTTCGCCTGC GTCAGGACTG AGCAAGGGTG 
     3481 AAAGAGACAG AGAGATCAAT TCCACGAATT TTGGAGAATG TCAGATTCCA GATTACCAGG 
     3541 AGCAGGACAT CTTCCTCTGG AGAAAAGAGA CCGGTTTTGG ATTTAGGATT CTGGGTGGAA 
     3601 ATGAGCCAGG GGAACCCATT TATATCGGTC ACATCGTACC ACTGGGTGCT GCTGACACAG 
     3661 ACGGCCGCCT GAGGTCTGGA GATGAATTAA TCTGTGTGGA TGGGACACCA GTAATTGGGA 
     3721 AATCACACCA GCTCGTGGTC CAGCTTATGC AACAAGCTGC CAAGCAAGGC CACGTCAATC 
     3781 TCACAGTGAG GCGTAAAGTG GTGTTTACGG TCCCCAAAGC AGAGAACGAG GTGCCCTCAC 
     3841 CAGCCTCATC GCACCACAGT AGCAACCAGC CAGCGTCCCT GACGGAAGAG AAGCGCACAC 
     3901 CGCAGGGCAG CCAGAACTCG CTGAACACCG TGAGCTCAGG CAGCGGCAGC ACCAGCGGCA 
     3961 TTGGCAGTGG TGGTGGCGGA GGCAGCGGCG TGGTGAGCGC TGTGCTGCAG CCATATGATG 
     4021 TGGAGATTCG GCGTGGGGAG AACGAGGGCT TTGGGTTTGT CATCGTGTCC TCCGTGAGCA 
     4081 GGCCCGAAGC GGGCACCACC TTCGCAGGCA ATGCATGTGT GGCTATGCCT CACAAAATAG 
     4141 GTCGGATTAT TGAGGGGAGC CCTGCTGACC GCTGTGGCAA GCTGAAAGTA GGAGACCGGA 
     4201 TCTTGGCGGT AAATGGATGT TCCATCACCA ACAAATCCCA TTCTGACATT GTCAACCTAA 
     4261 TCAAAGAAGC AGGCAACACA GTGACTCTCC GCATCATCCC CGGGGATGAG TCCTCCAATG 
     4321 CCACACTGCT GACTAATGCG GAGAAGATTG CCACCATCAC CACCACTCAT GCCCCCTCAC 
     4381 AACAGGGGAC CCAGGAAACA AGGACCACCA CCAAACCAAA GCCGGATTCT CAGTTTGAGT 
     4441 TCAAAGGACC GCAGGCTACA CAGGAGCAAG ATTTTTACAC TGTGGAATTG GAAAGAGGAG 
     4501 CCAAGGGATT TGGCTTTAGT CTTCGAGGAG GGCGAGAGTA TAACATGGAC CTTTATGTTC 
     4561 TGCGCTTAGC AGAGGATGGT CCTGCAGAAA GATGCGGAAA GATGAGGATT GGCGATGAAA 
     4621 TTCTAGAGAT CAATGGTGAG ACCACCAAAA ACATGAAACA CTCTCGGGCC ATAGAACTGA 
     4681 TCAAGAATGG CGGCCGCAGA GTCCGTCTGT TTCTGAGGCG GGGAGACGGC TCGGTCCCAG 
     4741 AATATGGTGG GTCAAACTAT GAAAACATCC CTTCCTTCCC TGGCATGACT CCAACGCGTA 
     4801 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4861 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4921 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4981 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5041 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5101 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5161 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5221 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5281 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5341 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5401 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5461 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5521 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5581 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5641 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5701 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5761 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5821 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5881 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5941 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6001 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6061 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6121 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6181 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6241 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6301 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6361 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6421 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6481 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6541 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6601 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6661 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6721 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6781 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6841 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6901 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6961 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7021 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7081 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7141 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7201 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7261 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7321 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7381 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7441 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7501 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7561 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7621 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7681 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7741 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7801 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7861 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8221 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8281 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8341 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8401 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8461 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8521 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8581 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8641 AATTTT
//