LOCUS       RR202612                8286 bp    DNA     circular  SYN 
DEFINITION  Rbl2 (Myc-DDK-tagged ORF) - Rat retinoblastoma-like 2 (Rbl2), (10 ug)
ACCESSION   NM_031094
VERSION     NM_031094.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Rbl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCTGGA GGCAACCAGT CGTCACCTCC TCCTCCTGCT GCTGCAGCCA 
     1081 GCTCAGAGGA AGAGGAGGAG GATGGCGACA CCGCGGACCG GGCGCAGCCC GCAGGGTCCC 
     1141 CAAGCCATCA GATCCAACAG CGGTTCGAAG AGCTGTGCAG CCGCCTTAAC ATGGACGAGG 
     1201 CGGCACGGGC CGAGGCCTGG AGCAGCTACC GCAGCATGAG CGAGAGCTAC ACGCTGGAGG 
     1261 GAAATGATCT CCATTGGTTA GCATGTGCCT TATATGTGGC TTGCAGAAAA TCTGTTCCTA 
     1321 CTGTGAGCAA AGGGACTGCT GAAGGAAACT ATGTGTCTTT AACTAGAATC CTTCGATGTT 
     1381 CTGAGCAAAG CCTAATTGAA TTTTTTAACA AGATGAAGAA GTGGGAAGAT ATGGCAAATT 
     1441 TACCCCCACA CTTCCGAGAA CGAACCGAAA GATTAGAAAG AAACTTCACT GTCTCCGCTG 
     1501 TGATCTTTAA GAAATATGAA CCCATTTTTC AAGACATTTT TAAATATCCC CAAGAAGAAC 
     1561 AACCTCGCCA GCAAAGAGGA AGAAAGCAGA GGCGACAGCC CTGTACCACA TCTGAAATTT 
     1621 TCCATTTTTG TTGGGTGCTT TTTATATACG CAAAAGGAAA CTTCCCCATG ATCAGTGATG 
     1681 ATCTGGTCAA TTCCTACCAT CTTCTGCTGT GTGCACTAGA TTTAGTTTAT GGAAATGCCC 
     1741 TACAATGCTC TAACCGGAAA GAACTTGTGA ACCCTAATTT TAAAGGCGTG TCCGAGGACG 
     1801 GCCACCCCAG GGACTCTCAC CCGTCCTCTG ATCCACCGTG TGTCATTGAG AAGCTCTGCT 
     1861 CCTTACACGA CGGCCTAGTG CTGGAGGCAA AGGGGATAAA GCAGCATTTC TGGAAACCCT 
     1921 ACATTAGGAA GCTTTTTGAA AAGAAGCTCC TCAGGGGGAA GGAAGAAAAT CTCACTGGCT 
     1981 TCCTGGAGCC CGGGAACTTC GCAGAGAGCT TTAAGGCTGT TAATAAGGCG TATGAAGAAT 
     2041 ACGTGTTAGC CACTGGGAGT CTGGATGAGC GGATATTTCT TGGCGAGGAT GCGGAGGAGG 
     2101 AAGTTGGAAC TTTCTCACGG TGTGTAAGTG CTGCCTCGGG CACAGAGAGT GCCGAACGAA 
     2161 CGCAGATGAG AGACATCTTG CAGCAGCATC TTGACAAGTC TAAAACACTT AGAGTCTGCA 
     2221 ACCCACTGAC TGGCGTGCGG TATGTTCAGG AGAACAGCCC TTGTGTGACT CCCGTCTCCA 
     2281 CAGCAACACA CAGCCTGAAC CGTCTTCACA CCATGCTGGC CGGCCTCAGG AATGCACCCA 
     2341 GTGAGAAGCT AGAACAGATA CTCAGGTCAT GTTCCCGAGA TCCGACTCGG GCTATCGCTG 
     2401 ACAGGTTGAG AGAGATGTAT GAAATATATT CTCAGCATTT CCAGCCAGAC GAGAATGTTA 
     2461 GTAATTGTGC TAAAGAAATG GCCAACAAAC ACTTTCGTTT TGCAGAAATG CTTTACTATA 
     2521 AAGTATTAGA GTCTGTTATT GAGCAAGAAC AGAAGAGATT GGGAGACATG GACTTATCTG 
     2581 GTGTTCTGGA GCAAGATGCA TTCCATAAGT CACTCCTGGC CTGCTGCCTT GAGGTGGTCG 
     2641 CTTTTTCCTA TAAGCCTCCT GGAAATTTCC CATTTATTGC TGAAATATTT GATGTACCAC 
     2701 ATTATCATTT TTATAAGGTA ATAGAAGTAT TTATTAGAGC AGAAGACGGT CTTTGCAGAG 
     2761 AAGTGGTCAA ACACCTCAAT CAGATTGAAG AACAAATTTT AGACCACTTG GCATGGAAAA 
     2821 CCAAGTCCCC ACTGTGGGAT AGAATTAGAG ATAATGAAAA CAGAGTCCCT ACATGTGAAG 
     2881 AGGTTACACC ACCTCACAAC CTAGAGAGAA CAGATGAAAT CTATATTGCT GGCTCTCCCT 
     2941 TAACTCCGAG AAGGGTGGGT GAAGTTCGCA CTGATGCTGG AGGACTTGGA AGAAGTGTGA 
     3001 CATCTCCAAC CACACTGTAT GACAGGTACA GTTCCCCAAC AGTCAGTACT ACTAGAAGGC 
     3061 GGCTATTTGA GAGTGACAGT CCCTCTGAAG GAAGCACGGC TGGGCGCATC CCCCCACAAC 
     3121 CCCTAGTCAA TGCTGTCCCT GTGCAGAATG TATCTGGGGA GACTGTTTCT GTCACACCAG 
     3181 TTCCTGGACA GACCTTGGTC ACCATGGCAA CAGCCACTGT CACAGCCAAC AATGGACAAA 
     3241 CAGTGACCAT TCCAGTACAA GGTATTGCCA ACGAAAATGG AGGGATAACA TTCTTCCCAG 
     3301 TCCAAGTGAA TGTTGGGGGC CAGGCACAGG CTGTGACTGG CTCTATCCAG CCCCTCAGTG 
     3361 CTCAAGCGCT GGCTGGAAGT CTGAGTTCCC AACAGGTGAC AGGAACAACT TTGCAAGTCC 
     3421 CCGGTCCGGT GGCCATTCAA CAAATTTCCC CTGGAGGACA ACAGCAGAAC CAAGGCCAGC 
     3481 CGTTAACCAG CAGCAGTATC AGGCCTCGGA AGACTAGCTC CTTATCACTT TTCTTTAGAA 
     3541 AGGTTTACTA CTTAGCTGGT GTCCGCCTTC GAGACCTTTG TACAAAACTA GATATTTCAG 
     3601 ATGAACTGAG GAAAAAAATT TGGACCTGCT TTGAATTCTC TATAGTCCAG TGTCCTGAAC 
     3661 TTATGATGGA CAGACATCTG GACCAGTTGT TAATGTGTGC CATTTACGTG ATGGCAAAGG 
     3721 TCACAAAAGA AGACAAGTCC TTCCAGAATA TCATGCGCTG TTACAGGACT CAGCCACAGG 
     3781 CCCGGAGCCA GGTATACAGA AGTGTCTTGA TAAAAGGGAA AAGAAGAAAT TCTGGCAGCT 
     3841 GTGAGAACAG AAGCCATCAG AATTCTCCAA CAGAACTAAA TACAGACAGA GCCAGTAGAG 
     3901 ACTCCAGTCC AGTTATGAGG TCAAACAGCA CCTTACCAGT TCCACAGCCC AGCAGTGCCC 
     3961 CTCCTACACC AACTCGACTC ACAGGTGCCA ACAGTGACAT TGAAGAGGAG GAAAGAGGAG 
     4021 ACCTCATTCA GTTCTACAAC AACATCTACA GGAAACAAAT CCAAACGTTT GCCATGAAGT 
     4081 ACTCACAGGC AAACTCCCAA ATGGACACCC CTCCCCTGTC TCCCTATCCG TTTGTAAGAA 
     4141 CGGGCTCCCC TCGCCGAGTA CAGTTATCTC AAAGTCACCC TATCTACATT TCCCCACATA 
     4201 AAAACGAAGC AATGCTTTCT CCTCGAGAGA AGATTTTTTA CTACTTCAGC AACAGCCCAT 
     4261 CAAAGAGACT GAGAGAAATT AACAGTATGA TACGGACAGG AGAGACTCCA ACTAAAAAGA 
     4321 GAGGGATTCT CTTGGACGAT GGAAGTGAAT CGCCTGCAAA AAGAATTTGC CCAGAAAATC 
     4381 ATTCTGCTCT GTTACGTCGT CTCCAAGATG TAGCTAATGA CCGAGGTTCA CACACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//