LOCUS       RR202507                7617 bp    DNA     circular  SYN 
DEFINITION  Lig4 (Myc-DDK-tagged ORF) - Rat ligase IV, DNA, ATP-dependent (Lig4), (10 ug)
ACCESSION   NM_001106095
VERSION     NM_001106095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..3865 
                     /label="Myc-DDK"  
     misc_feature    3895..4480 
                     /label="PolyA signal"  
     misc_feature    3952..3971 
                     /label="Restriction Enzyme"  
     misc_feature    4756..5428 
                     /label="ColE1"  
     misc_feature    5858..6652 
                     /label="Kan/Neo"  
     misc_feature    6687..6989 
                     /label="SV40 ori"  
     misc_feature    7075..7552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3756..3762 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3756 
                     /label="Lig4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCCTCA CAAGCCTCAC GAACTGTCGC AGCTCATGTC CCCTTTGCAG 
     1081 ACTTGTGCTC CACCTTAGAA CGAATACAGA AAGGTAAAGA CCGTGCAGAA AAAATCAGGC 
     1141 ACTTCAAGGA GTTTCTGGAT TCTTGGAGAA AATTTCATGA TGCCCTTCAT AAGAACAAGA 
     1201 AGGATGTTCC GGACTCTTTT TACCCAGCAA TGAGACTTAT TCTCCCTCAG CTAGAGAGAG 
     1261 AGAGGATGGC TTACGGAATC AAAGAAACCA TGCTGGCTAA GCTTTACATC GAATTGCTCA 
     1321 ATTTACCAAG AGAAGGCAAG GATGCCCAGA AGCTCCTGAA TTACCGAACC CCCAGTGGAG 
     1381 CTCGCTCAGA TGCTGGGGAC TTTGCCACGA TTGCATACTT TGTTTTGAAG CCAAGGTGCT 
     1441 TACAGAAAGG AAGCTTAACC ATACAGAAGG TGAATGAACT CTTAGACTTA GTTGCCAGCA 
     1501 ATAACTCTGG CAAAAGAAAA GATCTAGTGA AAAAGAGCCT TCTTCAGTTA ATAACCCAGT 
     1561 GTTCAGCACT CGAGCAAAAG TGGCTGATTC GCATGATTAT TAAGGACTTA AAGCTTGGTG 
     1621 TCAGTCAGCA CACGATATTG TCCATCTTCC ACAATGATGC AGTCGAGTTG CACAATGTCA 
     1681 CCACAGATCT GGAAAAGGTC TGTAGGCAGC TACATGACCC CTCCGTAGGG CTCAGCGACA 
     1741 TCTCCATCAC TTTGTTCTCT GCCTTTAGGC CAATGCTAGC TGCTGTAGCA GATGTGGAGC 
     1801 GTGTGGAGAA GGACATGAAG CAGCAGAGTT TCTACATCGA AACTAAGCTC GATGGCGAGC 
     1861 GCATGCAGAT GCACAAGGAT GGCGCCCTGT ACCGGTACTT CTCCAGAAAT GGTTACAACT 
     1921 ACACCGATCA GTTCGGCGAA TCTCCACAGG AAGGCTCTCT CACCCCGTTT ATTCACAACG 
     1981 CATTCAAGAC AGATGTGCAA GTGTGCATCC TTGATGGCGA GATGATGGCC TACAACCCAA 
     2041 CGACACAGAC TTTCATGCAG AAGGGGGTCA AGTTTGATAT CAAAAGGATG GTCGAAGATT 
     2101 CTGACCTACA GACATGCTAC TGTGTTTTCG ATGTGCTGAT GGTTAATAAT AAGAAGCTAG 
     2161 GGCGTGAGAC TCTCAGGAAG AGGTATGATA TCCTTAGTAG CACTTTCACA CCCATACAAG 
     2221 GTCGAATAGA GATAGTGCAG AAAACTCAAG CTCAGACAAA GCAGGAAGTA GTGGATGCAT 
     2281 TAAATGATGC CATAGACAAG AGAGAAGAGG GGATCATGGT TAAACACCCT CTGTCCATTT 
     2341 ACAAGCCAGA CAAAAGAGGT GAAGGGTGGC TAAAGATTAA ACCAGAGTAT GTCACTGGAT 
     2401 TGATGGATGA ATTAGACCTC TTAATTGTTG GGGGCTACTG GGGTAAAGGT TCTCGAGGTG 
     2461 GCATGATGTC TCACTTTCTG TGTGCAGTGG CAGAGACACC ACCTCCTGGT GACAAGCCAT 
     2521 CTGTGTTCCA CACTCTGTGC CGTGTTGGGT CGGGTTACAC GATGAAAGAA CTCTATGACC 
     2581 TGGGCTTGAA ATTGGCAAAA TACTGGAAGC CTTTCCAAAG AAAATCTCCA CCAAGCTGTA 
     2641 TTTTGTGTGG CACAGAGAAG CCAGAAGTGT ACATTGAACC CCAGAACTCT GTCATCGTTC 
     2701 AGATCAAGGC GGCAGAGATC GTCCCCAGTG ACATGTACAA GACTGGCTCT ACCTTGCGCT 
     2761 TCCCACGCAT CGAGAAGATC AGAGATGACA AAGAGTGGCA TGACTGTATG ACGCTGGATG 
     2821 ACCTGGAGCA GCTGAGGGGG AAAGCATCTG GGAAGCTTGC CACAAAACAC CTTCACATAG 
     2881 GTGACGATGA CGAACCCAGA GAAAAAAGGC GGAAACCCAT CTCTAAGACG AAGAAAACCA 
     2941 TTGGAATCAT TGAACACCTA AAAGCGCCTA ACCTTTCTAA CATAAACAAA GTTTCTAATG 
     3001 TATTTGAAGA TGTTGAGTTT TGTGTCATGA GTGGATTAGA TGGTTATCCA AAGCCTGACC 
     3061 TAGAGAACAA AATTGCAGAA TTTGGTGGTT ATATAGTCCA GAATCCAGGC CCAGATACAT 
     3121 ACTGTGTGAT TGCAGGTTCT GAGAACATTA GGGTCAGAAA CATCATCTCT TCAGATAGGC 
     3181 ATGATGTTGT CAAGCCCGAG TGGCTTTTAG AGTGTTTTAA AACAAAAACA TGTGTGCCAT 
     3241 GGCAACCCCG CTTTATGATT CACATGTGCC CATCAACAAA GCAGCATTTT GCCCGTGAAT 
     3301 ATGACTGCTA TGGTGATAGC TATTTCGTTG ATGCAGATTT GGATCAATTG AAAGAAGTGT 
     3361 TTTTAAGAAT TAAACCCAGT GAGCAGCAGA CTCCTGAAGA AATGGCCCCT GTGATTGCTG 
     3421 ACCTAGAATG TCGTTATTCC TGGGATCACT CTCCTCTCAG TATGTTTCGA CACTACACCG 
     3481 TTTATTTGGA CTTGTATGCT GTTATTAATG ACTTAAGTTC CAAAATCGAA GCCACAAGGT 
     3541 TAGGTGTTAC AGCACTTGAG CTGCGGTTTC GTGGAGCAAA GGTGGTTTCC CGCTTATCTG 
     3601 AAGGGGTGTC TCACGTAATC ATCGGGGAAG ATCAGAGACG AGTCAGTGAC TTTAAAACGT 
     3661 TCAGAAGAAC GCTTAAGAAA AAGTTTAAAA TCCTGCAGGA ACGTTGGGTG ATCGATTCAG 
     3721 TAGACAAGTG TGAACTGCAG GAAGAAAACC TGTATTTGCT TCTTACGCGT ACGCGGCCGC 
     3781 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3841 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3901 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3961 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4021 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4081 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4141 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4201 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4261 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4321 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4381 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4441 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4501 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5401 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5461 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5521 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5581 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5641 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5701 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5761 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5821 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5881 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5941 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6001 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6061 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6121 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6181 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6241 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6301 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6361 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6421 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6481 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6541 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6601 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6661 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6721 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6781 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6841 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7021 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7081 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7141 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7201 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7261 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7321 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7381 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7441 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7501 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7561 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//