LOCUS       RR202488                8622 bp    DNA     circular  SYN 
DEFINITION  Smc1b (Myc-DDK-tagged ORF) - Rat structural maintenance of chromosomes 1B (Smc1b), (10 ug)
ACCESSION   NM_001130498
VERSION     NM_001130498.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4779..4870 
                     /label="Myc-DDK"  
     misc_feature    4900..5485 
                     /label="PolyA signal"  
     misc_feature    4957..4976 
                     /label="Restriction Enzyme"  
     misc_feature    5761..6433 
                     /label="ColE1"  
     misc_feature    6863..7657 
                     /label="Kan/Neo"  
     misc_feature    7692..7994 
                     /label="SV40 ori"  
     misc_feature    8080..8557 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4761..4767 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4761 
                     /label="Smc1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCACCTT GAGTTGCTGC TCGTGGAGAA TTTCAAGTCG TGGCGAGGCC 
     1081 GCCAGGTCAT CGGCCCTTTC AAGAGGTTCA CCTGTATCAT CGGCCCCAAC GGCTCGGGAA 
     1141 AGTCTAATGT AATGGATGCA CTTAGTTTTG TAATGGGAGA AAAAACAACT AATTTAAGAG 
     1201 TAAAAAACAT TCAAGAACTT ATTCATGGAG CACACATTGG AAAACCTGTT TCTTCTTCTG 
     1261 CAAGTGTGAC CATTATATAC GTAGAAGACA GTGGAGAAGA GAAAACATTC ACAAGGATTA 
     1321 TCCGAGGGGG GTGCTCAGAA TTTCATTTTG GGGATAAACC TGTGAGTCGT TCTGTGTATG 
     1381 TAGCCCAGTT GGAAAACATA GGCATAATAG TCAAAGCACA GAACTGTCTA GTTTTTCAGG 
     1441 GAACTGTAGA GTCAATATCC ATGAAAAAAC CCAAAGAGAG AACCCAATTT TTTGAAGAAA 
     1501 TCAGTACTTC AGGAGAGTTT ATAGGAGAAT ATGAGGCAAA GAAAAAGAAG TTACAAAAAG 
     1561 CCGAAGAAGA TGCACAATTT CATTTTAACA GGAAAAAAAA TGTAGCTGCA GAACGGAAGC 
     1621 ATGCAAAAAT AGAGAAAGAA GAGGCAGAAC ATTATCAAAG TCTCCTTGAA GAATTGAAAA 
     1681 CAAACAAGAT ACAACTGATG CTTTTCCAAC TATATTATAA TGAGGAAAAA ATCAATGTTT 
     1741 TAAACACTGA ATTAGAGCAT ATGGACAGGA ATTTAAGTGT TGTAAAAGAC ACTCTTTCTC 
     1801 ACCATGAAAA CATAATTAAA GCTAAGAAAA AGGATTATGG AATGTTAACT AGACAACTAC 
     1861 AGCAAACAGC AAAAGAACTG AAATCTGTCG AAGCAGTTTT AAATCAGAAG AGGCCTCAGT 
     1921 ACATTAAAGC TAAAGAAAAC ACTTCTCACC ATCTAAAAAA ATTGGATTTG TCTAAGAAAT 
     1981 TGATAACAGA CAATGAAAAA CAATGTGCTA AGCAGGAAGA TGGCATTCGA GCCTTAGTGG 
     2041 CAGAACTGGT TGATTTAGAT AGAGCATGGA GAAGTTTTGA AAAGCAGATG GAAGAGAAAA 
     2101 TCTTACACAA AGGAAGAGAC ATTGAATTGG AAAATAGTCA GCTGGACCGT TATAAAGAGC 
     2161 TTAAGGAACA AGTCAGACGG AGGGTAGGTA TAATGACACA ACAACTGGAA AAACTACAGT 
     2221 GGGAACAGAA GGCAGAAAAA GAAAGACTGG CATTTGAAAG GAGGAGGCAT GGAGACACTC 
     2281 AGGGAAATCT AAAACAAATA AAAGAACAAA TAGAAGAGCA TAAAAAACGA ATAGAGAAAT 
     2341 TGGAGGAATA TACAAAGACG TGCATGGATT GCTTGGAAGA TAAAAAACAG CAAGAAGAAG 
     2401 CCCTGATAAA AGAAATTGAC AATACAAAAT CAAGAATGTC TGAAGTTAAT GAAGAATTGA 
     2461 GTCTTATTAG AAATGAATTG CAGAATGCTG GAATTGATAA CCATGAAGGA AAACGTCAGC 
     2521 GGAAAAGAGC AGAAGTTCTC GAACACCTTA AAAGACTTTA CCCAGATTCT GTGTTTGGAA 
     2581 GACTTCTTGA TCTATGTCAT CCTATTCATA AGAAATACCA GCTGGCTGTG ACTAAGCTTT 
     2641 TTGGCCGGTA CATGGTTGCC ATTGTTGTTG CCTCAGAAAA GGTAGCAAAA GATTGTATTC 
     2701 GGTTTCTGAA GGAGGAAAGA GCTGAACCTG AGACATTCCT TGCTCTAGAT TACCTTGATA 
     2761 TCAAGCCAAT CAATGAACGA CTAAGGGAAA TTAAAGGCTG TAAGATGATG ATCGATGTTA 
     2821 TAAAGACCCA GTTTCCTCAG CTGAAGAAAG TGATTCAGTT TGTTTGTGGA AATGGCCTTG 
     2881 TCTGTGAGAC TGTGGAAGAG GCAAGACATA TTGCATTTGG TGGACCTGAA AGACGAAAGG 
     2941 CAGTAGCACT TGATGGAACA CTGTTTTTGA AATCTGGAGT GATCTCAGGA GGGTCAAGTG 
     3001 ACTTAAAGCA CAAAGCTCTG TGTTGGGATG AGAAGGAGTT ACACAGTCTA AGAGATAAAA 
     3061 GAAACCAACT TGTCCAAGAG CTAAAGGAGT TAATGAAGAC GCTCCGCAAG GAAACAGATC 
     3121 TGAAACAAAT ACAGGCATTA GTACAAGGAA CCAATACACG ACTCAAATAT TCACAAAATG 
     3181 AACTAGAGAT GATTAAAAAG AAGCACCTTG CTACATTTTA CCGGGAACAA TCTCAGCTAC 
     3241 AAAGTGAATT ACTGAATATT GATTCTCAGT GTACAATGTT GAGCGAAGGA ATCAACAAAC 
     3301 AGCAACAAAA GATTGAAGAA TTTCAAGATA AGATAGACAA GGTAGAAGAT GACATATTCC 
     3361 AAGACTTCTG TGAAGAAATT GGTGTGGAAA ATATCCGTGA ATTTGAGAAC AAACATGTTA 
     3421 AACAGCAGCA AGAAAATGAT CAAAAAAGAT TAGAGTTTGA AAAACAAAAA ACTCGGCTCA 
     3481 ATATTCAGCT TGAATATAGT CGAAATCAAC TTAAGAAGAA ACTGAATAAG ATCGACACAT 
     3541 TAAAAACAAC AATCCAGAAG GGCAGAGAAG ACATTGATAA CCTAAAGAAG ACCGAAGAAG 
     3601 AGTGTCTGAA AATTGTTGAA GAACTCATGG TGAAACAAGA ACAAGTTAAG GAAGTGCTTG 
     3661 CCACACAGAG TTCCAACATT GAGAAAATTC ATATTCAAAT TGAAGAGGAA CGCAAGAAGT 
     3721 TTTTGGCTGT TGATAGGGAA GTTGGAAAAT TACAGAAGGA AGTTGTAATC ATTCAGGGTT 
     3781 CTTTGGAACA GAAACAACTA GAGAAGCATA ACTTGCTCCT GGACTGCAAA GTTCAAGACA 
     3841 TTGACATAAG TCTCATGCTG GGGTCATTGG AGGACATCAT TGAAGTGGAG CTAACTGAAA 
     3901 CAGAAAGTAC CCAGGCAACG GCTGATATCT ATGAAAAAGA AGCATCCATC CAAATAGACT 
     3961 ACAGCCCTCT AAGGGAGGAT TGGAAGGCTC TACAATCAGA TAAGGAGGTG GAGGCCCACC 
     4021 TCACACTCCT TCTACAGCAA GTAGCATCCC AAGAAAATAC TCTACTGAAG ACTGCAGCTC 
     4081 CAAACCTGCG AGCACAGGAG AACTTAAAGG CTGTCAGAGA CAAATTTCAA GAATCTGCAG 
     4141 ATGCTTTTGA GGCCAGCAGA AAGGAAGCCA GAATATGTAG GCAAGAGTTT GAACAGGTGA 
     4201 AAAGACGGAG GTACGATGCT TTCAGTCAGT GTTTTGAGCA CATCTCGGTC TCAATTGATC 
     4261 AAATCTACAA GAAGCTCTGC AGGAACAACA GTGCCCAGGC ATTTCTTAGC CCAGAGAACC 
     4321 CTGAAGAACC TTACTTAGAT GGAATTAGCT ATAATTGTGT GGCTCCAGGC AAACGGTTCA 
     4381 TGCCTATGGA CAACTTGTCA GGGGGAGAAA AGTGTGTGGC TGCTCTGGCT CTTCTGTTTG 
     4441 CTGTACACAG TTTTCGACCT GCTCCATTCT TTGTATTAGA TGAAGTAGAT GCAGCCCTGG 
     4501 ACAATACTAA CATCGGCAAA GTCTCAGGTT ACATCAAAGA GCAGAGTCAG GAACAATTTC 
     4561 AGATGATAAT CATTTCCCTG AAAGAGGAGT TTTACTCCAG AGCTGATGCA CTGATAGGCG 
     4621 TCTACCCAGA GCACGATGAA TGCATGTTCA GCCATGTGTT GACTCTGGAC CTTTCCAAGT 
     4681 ATCCAGACAC TGAAGACCAA GAAGGCAGCA GGAGCCACCG GAAACCCAGA GCACGAGTAT 
     4741 CACAGTCCCC GCAGTCTCCC CAGTCTCGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4801 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4861 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4921 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4981 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5041 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5101 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5161 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5221 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5281 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5341 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5401 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5461 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5521 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5581 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5641 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5701 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5761 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5821 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5881 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5941 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6001 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6061 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6121 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6181 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6241 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6301 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6361 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6421 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6481 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6541 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6601 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6661 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6721 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6781 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6841 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6901 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6961 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7021 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7081 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7141 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7201 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7261 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7321 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7381 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7441 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7501 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7561 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7621 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7681 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7741 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7801 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7861 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7921 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8041 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8101 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8161 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8221 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8281 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8341 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8401 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8461 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8521 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8581 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//