LOCUS       RR202446                9216 bp    DNA     circular  SYN 
DEFINITION  Dcc (Myc-DDK-tagged ORF) - Rat deleted in colorectal carcinoma (Dcc), (10 ug)
ACCESSION   NM_012841
VERSION     NM_012841.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5373..5464 
                     /label="Myc-DDK"  
     misc_feature    5494..6079 
                     /label="PolyA signal"  
     misc_feature    5551..5570 
                     /label="Restriction Enzyme"  
     misc_feature    6355..7027 
                     /label="ColE1"  
     misc_feature    7457..8251 
                     /label="Kan/Neo"  
     misc_feature    8286..8588 
                     /label="SV40 ori"  
     misc_feature    8674..9151 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5355..5361 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5355 
                     /label="Dcc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAATAGT CTTGGATGTG TCTGGGTACC CAAGCTGGCT TTTGTACTCT 
     1081 TCGGAGCTTC CTTGCTCAGC GCGCATCTTC AAGTCACGGG TTTCCAAATT AAACCTTTCA 
     1141 CGTCACTTCA TTTTGTGTCT GAACCTTCTG ATGCTGTCAC AATGCGGGGA GGAAACGTCC 
     1201 TGCTCAACTG CTCTGCAGAG TCTGACCGCG GAGTCCCAGT CATCAAGTGG AAGAAAGATG 
     1261 GCCTTATCCT GGCCTTGGGA ATGGATGACA GAAAGCAGCA ACTTCCTAAT GGGTCTCTGT 
     1321 TGATACAAAA CATACTTCAT TCCAGACACC ATAAACCGGA TGAAGGACTT TACCAATGCG 
     1381 AGGCATCCTT AGGAGACTCT GGCTCAATTA TTAGTCGGAC AGCAAAAGTT ATGGTAGCAG 
     1441 GACCACTGAG GTTCCTCTCC CAGACAGAAT CTATCACAGC CTTCATGGGA GATACAGTGT 
     1501 TACTTAAGTG TGAAGTCATT GGGGACCCCA TGCCAACAAT ACACTGGCAG AAGAATCAAC 
     1561 AAGACCTCAA CCCGATCCCA GGAGACTCGC GTGTGGTCGT CTTGCCCTCC GGAGCATTGC 
     1621 AGATCAGCCG ACTTCAACCA GGAGACAGTG GAGTCTACCG ATGCTCAGCT CGGAATCCAG 
     1681 CCAGCACAAG AACAGGAAAT GAAGCCGAAG TCAGGATTTT ATCAGACCCA GGCCTGCATA 
     1741 GGCAGCTGTA TTTTCTGCAG AGACCATCCA ATGTGATAGC CATTGAAGGG AAAGATGCTG 
     1801 TCCTGGAGTG TTGTGTTTCT GGCTATCCTC CACCAAGTTT CACCTGGTTA CGGGGCGAGG 
     1861 AGGTCATCCA ACTCAGGTCT AAAAAGTATT CGTTGTTGGG TGGGAGCAAT TTGCTCATCT 
     1921 CTAATGTGAC AGATGATGAC AGTGGGACCT ATACGTGCGT TGTCACATAT AAGAATGAGA 
     1981 ATATTAGTGC CTCTGCAGAG CTCACGGTCT TGGTTCCGCC ATGGTTTTTA AATCACCCTT 
     2041 CCAACCTCTA TGCCTATGAA AGCATGGACA TTGAGTTTGA ATGTGCAGTG TCCGGGAAGC 
     2101 CTGTGCCCAC TGTGAATTGG ATGAAGAATG GAGATGTGGT GATTCCTAGT GATTACTTTC 
     2161 AGATAGTGGG AGGAAGCAAC TTACGAATCC TTGGGGTGGT GAAATCAGAT GAAGGCTTTT 
     2221 ATCAATGTGT GGCTGAAAAT GAGGCTGGCA ATGCCCAAAG TAGTGCCCAG CTGATTGTCC 
     2281 CCAAGCCTGC CATCCCAAGC TCCAGCATCC TCCCTTCTGC TCCCAGAGAT GTGGTCCCTG 
     2341 TCTTGGTTTC TAGTCGCTTT GTCCGTTTGA GCTGGCGTCC ACCTGCCGAA GCCAAAGGAA 
     2401 ACATTCAAAC ATTCACAGTC TTTTTCTCCA GAGAGGGTGA CAACAGGGAG CGAGCTTTGA 
     2461 ATACAACCCA GCCTGGGTCC CTTCAGCTTA CTGTGGGAAA CTTGAAACCA GAAGCCATGT 
     2521 ATACCTTCAG AGTTGTTGCC TACAATGAGT GGGGACCAGG GGAGAGCTCT CAACCCATCA 
     2581 AGGTGGCCAC TCAGCCTGAG TTGCAAGTTC CAGGGCCAGT AGAAAACCTG CACGCTGTAT 
     2641 CTGCCTCTCC TACCTCAATT CTTATTACAT GGGAACCCCC TGCCTATGCA AATGGTCCGG 
     2701 TTCAAGGTTA CAGATTGTTT TGCACTGAGG TGTCTACAGG AAAAGAACAG AATATAGAGG 
     2761 TTGATGGACT ATCTTATAAA CTGGAAGGCC TGAAAAAATT CACGGAATAT ACTCTTCGAT 
     2821 TCCTTGCTTA TAATCGTTAT GGTCCGGGAG TGTCTACCGA TGACATAACA GTCGTTACAC 
     2881 TTTCAGATGT GCCAAGTGCT CCACCTCAGA ACGTCTCTCT GGAAGTGGTT AATTCAAGGA 
     2941 GTATCAAAGT AAGCTGGCTC CCTCCTCCAT CGGGAACACA AAATGGATTT ATTACTGGCT 
     3001 ATAAAATTCG ACACAGAAAG ACTACCCGCA GGGGTGAGAT GGAAACACTG GAACCCAATA 
     3061 ACCTCTGGTA CCTCTTCACA GGATTGGAGA AGGGGAGTCA GTACAGTTTC CAAGTGTCAG 
     3121 CCATGACTGT CAATGGCACT GGACCACCTT CTAACTGGTA CACAGCAGAA ACCCCAGAGA 
     3181 ACGACTTAGA CGAGTCTCAA GTTCCTGACC AGCCAAGTTC TCTCCATGTG AGGCCCCAGA 
     3241 CAAACTGCAT CATCATGAGT TGGACTCCAC CTCTCAACCC AAACATCGTG GTGAGAGGCT 
     3301 ATATTATCGG ATATGGTGTT GGCAGCCCTT ATGCCGAGAC AGTGCGTGTA GACAGTAAGC 
     3361 AGCGGTACTA TTCCATCGAG AGGCTGGAGT CGAGTTCTCA TTATGTAATC TCCTTAAAAG 
     3421 CTTTCAACAA TGCTGGAGAA GGTGTCCCTC TCTACGAAAG TGCCACTACC CGTTCCATAA 
     3481 CAGACCCCAC TGACCCCGTT GATTATTATC CTTTGCTTGA TGATTTCCCC ACCTCGGGCC 
     3541 CAGATGTCTC CACCCCCATG CTCCCACCAG TAGGTGTGCA GGCTGTGGCT CTTACCCACG 
     3601 AGGCTGTGAG GGTCAGCTGG GCAGACAACT CTGTCCCGAA GAACCAGAAG ACATCCGACG 
     3661 TTCGGCTTTA CACGGTTCGG TGGAGGACCA GCTTTTCCGC CAGTGCCAAG TACAAGTCAG 
     3721 AAGACACAAC GTCTCTCAGC TACACAGCGA CTGGCCTCAA GCCAAATACC ATGTATGAGT 
     3781 TCTCAGTCAT GGTGACAAAG AACAGAAGGT CAAGCACGTG GAGCATGACT GCACACGCCA 
     3841 CCACCTACGA AGCTGCCCCA ACTTCTGCTC CCAAGGATTT GACAGTCATT ACTCGTGAGG 
     3901 GGAAGCCTCG TGCTGTCATT GTGAGCTGGC AGCCTCCCTT GGAAGCCAAT GGGAAAATCA 
     3961 CCGCTTACAT CTTGTTTTAT ACCTTGGACA AGAATATACC CATCGATGAC TGGATTATGG 
     4021 AGACTATCAG CGGTGACCGG CTTACCCATC AGATCATGGA TCTGAGCCTG GACACTATGT 
     4081 ATTACTTTCG AATTCAAGCA CGGAACGCTA AAGGCGTGGG GCCCCTCTCG GATCCCATCC 
     4141 TCTTCAGGAC TTTGAAAGTG GAACACCCTG ACAAAATGGC TAATGACCAA GGTCGTCATG 
     4201 GAGATGGAGG TTATTGGCCA GTTGATACTA ACTTGATTGA TAGAAGCACT CTCAACGAAC 
     4261 CACCCATTGG ACAAATGCAC CCCCCACATG GCAGTGTAAC TCCTCAAAAG AACAGTAACC 
     4321 TGCTTGTCAT AACCGTGGTC ACCGTGGGCG TTCTCACCGT GTTGGTTGTG GTCATTGTAG 
     4381 CAGTGATTTG TACCCGGCGC TCTTCAGCCC AGCAAAGGAA GAAACGGGCC ACGCACAGTG 
     4441 CGAGCAAGAG GAAGGGCAGT CAGAAGGACC TCCGGCCTCC GGATCTTTGG ATACATCATG 
     4501 AAGAAATGGA AATGAAAAAT ATCGAGAAGC CGGCAGGAAC CGACCCTGCA GGAAGAGGCT 
     4561 CCCCCATCCA GAGTTGCCAA GACCTCACAC CAGTCAGCCA CAGCCAGTCA GAAAGCCAGA 
     4621 TGGGAAGCAA AAGTGCCTCC CATTCAGGTC AAGATACCGA GGAGGCAGGG AGCTCCATGT 
     4681 CTACTCTAGA AAGGTCCCTT GCAGCACGGC GGGCCACGAG GACCAAGCTC ATGATTCCCA 
     4741 TGGAGGCCCA GTCCAATAAT CCTGCTGTCG TGAGTGCCAT CCCTGTACCA ACCCTAGAAA 
     4801 GTGCCCAGTA TCCAGGAATC CTCCCGTCTC CCACATGTGG ATATCCGCAT CCACAGTTCA 
     4861 CTCTCCGGCC AGTACCATTC CCAACACTGT CTGTGGACCG AGGTTTCGGA GCAGGAAGAA 
     4921 CTGTGAGTGA AGGACCAACA GCACAACAAC AACCCATGCT GCCCCCAGCT CAGCCTGAAC 
     4981 ATCCCAGCAG CGAAGAAGCC CCCAGCAGAA CCATCCCTAC AGCCTGTGTG CGGCCAACTC 
     5041 ACCCACTCCG AAGTTTTGCA AACCCTTTGC TACCTCCACC TATGAGTGCA ATAGAACCAA 
     5101 AAGTCCCTTA CACACCGCTT TTGTCGCAGC CAGGACCCAC CCTTCCCAAG ACTCATGTTA 
     5161 AAACGGCCTC CCTTGGGCTA GCAGGAAAGG CAAGGTCCCC TTTGCTTCCC GTATCTGTGC 
     5221 CAACAGCCCC TGAAGTGTCT GAAGAGAGTC ACAAGCCAAC AGAGGATCCA GCCAGTGTAT 
     5281 ATGAACAGGA TGACTTGAGC GAACAAATGG CGAGTTTGGA AGGGCTAATG AAGCAACTCA 
     5341 ATGCCATCAC GGGCTCCGCC TTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5401 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5461 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5521 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5581 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5641 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5701 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5761 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5821 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5881 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5941 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6001 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6061 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6121 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6181 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6241 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6301 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6361 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6421 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6481 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6541 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6601 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6661 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6721 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6781 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6841 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6901 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6961 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7021 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7081 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7141 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7201 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7261 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7321 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7381 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7441 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7501 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7561 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7621 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7681 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7741 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7801 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7861 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7921 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7981 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8041 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8101 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8161 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8221 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8281 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8341 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8401 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8461 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8521 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8581 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8641 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8761 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8821 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8881 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8941 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9001 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9061 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9121 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9181 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//