LOCUS       RR202434                9210 bp    DNA     circular  SYN 
DEFINITION  Pappa (Myc-DDK-tagged ORF) - Rat pregnancy-associated plasma protein A (Pappa), (10 ug)
ACCESSION   NM_001107939
VERSION     NM_001107939.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5365..5456 
                     /label="Myc-DDK"  
     misc_feature    5486..6071 
                     /label="PolyA signal"  
     misc_feature    5543..5562 
                     /label="Restriction Enzyme"  
     misc_feature    6347..7019 
                     /label="ColE1"  
     misc_feature    7449..8243 
                     /label="Kan/Neo"  
     misc_feature    8278..8580 
                     /label="SV40 ori"  
     misc_feature    8666..9143 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5340..5347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5340 
                     /label="Pappa"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCATTCAC ACCATCAGTG ATCAAGGCAA CAGAGATCCA CGCTACTTTT 
     1081 TCTCCTTGAA GACAGACCGG GCCAGGAAAG TAACCACCAT TGATGCCCAT CGCAGCTACC 
     1141 TCCCGGGTCA GTGGGTGCAC CTAGCTGCTA CCTACGATGG GCGACTGATG AAGCTCTATA 
     1201 TGAATGGTGC CCAGGTGGCG ACCTCAGCGG AGCAAGTAGG TGGCATATTC AGCCCACTGA 
     1261 CCCAGAAGTG TAAAGTGCTT ATGTTGGGGG GCAGTGCTCT GAATCACAAC TTCCGGGGCC 
     1321 ACATTGAACA CTTCAGTCTA TGGAAAGTAG CCAGAACCCA GCGAGAGATA CTATCCGACA 
     1381 TGGAAACGCG TGGCCTCCAC ACCCCTCTAC CTCAGCTCCT CCTCCAGGAG AACTGGGACA 
     1441 ATGTGAAGCG CACTTGGTCC CCTATGAAGG ATGGCCACAG CCCCCAGGTG GAATTCAGCA 
     1501 ATGCACATGG CTTCCTGTTG GACACTAATT TGGAGCCCCC TCTTTGCGGG CAGACACTGT 
     1561 GTGACAACAC AGAGGTCATC TCCAGTTACA ATCAGCTCCC AAGTTTTCGG CAGTCCAAGG 
     1621 TGGTGCGCTA TCGTGTGGTC AACATCTATG ATGATCACCA TGAAAACCCT ACAGTGAGCT 
     1681 GGCAGCAGAT TGACTTTCAG CACCAACAGC TGGCTGAGGC CTTCCAGCAC TACAACATCT 
     1741 CCTGGGAGTT GGACGTACTA GATATAAACA GTTCCTCTCT GCGTCACCGC CTCATCCTGG 
     1801 CCAACTGTGA CATCAGCAAG ATTGGGGATG AAAAATGCGA CCCTGAATGT AACCATACAC 
     1861 TGACTGGCCA TGACGGTGGG GATTGCCGGC AGCTGCGCTA CCCTGCATTC ATGAAGAAGC 
     1921 AGCAGAATGG CGCATGTGAC ATGGACTGCA ACTACGAAAG GTTTAATTTT GATGGTGGAG 
     1981 AGTGCTGTGA CCCAGACATC ACTGATGTCA CTAAGACATG CTTTGATCCT GACTCTCCAC 
     2041 ACAGAGCCTA CTTGGATGTT AATGAGCTAA AGAACATTCT TAAACTGGAT GGATCAACAC 
     2101 ATCTCAATAT TTTCTTTGCA AACTCTTCAG AGGAGGAGTT GGCAGGAGTG GCAACTTGGC 
     2161 CATGGGACAA GGAGGCCCTA ATGCACTTGG GCGGTATTGT CTTGAACCCG TCTTTCTATG 
     2221 GCATTCCTGG ACACACCCAC ACCATGATCC ATGAGATTGG GCATAGCCTG GGCCTCTATC 
     2281 ACATCTTCCG TGGTATCTCA GAAATCCAGT CCTGCAGTGA TCCTTGCATG GAGACAGAGC 
     2341 CCTCATTTGA AACTGGAGAC CTCTGCAATG ACACCAACCC AGCCCCTAAA CACAAGTTTT 
     2401 GTGGAGACCC TGGACCAGGG AATGACACTT GTGGCTTTCA TGGCTTCTTC GACACTCCTT 
     2461 ATAACAACTT CATGAGCTAT GCAGATGACG ACTGTACAGA CTCTTTCACG CCCAATCAAG 
     2521 TCTCCAGAAT GCACTGTTAC CTGGACCTCG TATACCAGAG CTGGCAGCCC TCCAGAAAGC 
     2581 CAGCACCTGT AGCTCTTGCA CCCCAGATTG TGGGCCACAC AACCGACTCT GTGATGCTAG 
     2641 AGTGGTTCCC ACCCATCGAT GGCCACTTCT TTGAAAGAGA ATTGGGATCA GCATGTGACC 
     2701 TTTGCCTAGA AGGGAGAATC CTGGTGCAAT ATGCTTTCAA TGCTTCCTCC CCCATGCCCT 
     2761 GTGGACCATC GGGACACTGG AGTCCTCGGG AAGCAGAAGG TCACCCAGAT GTTGAACAGC 
     2821 CCTGTAAATC CAGTGTCCGT ACCTGGAGTC CAAATTCAGC TGTCAACCCA CACACAGTCC 
     2881 CTCCAGCCTG CCCTGAGCCA CAAGGCTGCT ACCTCGAGCT GGAATTCCGC TACCCCTTGG 
     2941 TCCCTGAGTC TCTGACCATC TGGGTAACCT TTGTCTCCAG TGACTGGGAC TCCAGTGGAG 
     3001 CTGTCAATGA CATCAAACTC TTGACTGTTA GTGGAAAGAA CATCTCTTTG GGTCCTCAAA 
     3061 ATGTCTTCTG TGATATCCCA CTTACCATCA GACTCCGAGA TGTGAGTGAG GAGGTATATG 
     3121 GCATCCAAAT CTATACTCTG GATGAACACC TGGAAATTGA TGCAGCCATG TTGACCTCCG 
     3181 CTGTAGACAG CCCACTCTGT CTACAGTGTA AACCCCTGCA ATACAAAGTG CTCAGAGACC 
     3241 CACCTCTGCT GGATGACGTA GGCTCATTAC TCCACCTCAA CAGAAGATTC ATGGATATGG 
     3301 ATCTGAAACT TGGCAATGTG TACCAGTACC GGATTATCAC CATATCAGGA AACGAAGAGA 
     3361 GTGAGCCCTC ACCTGCTGCC ATATACACCC ATGGAAGTGG GTACTGTGGT GATGGCGTTA 
     3421 TTCAAAAAGA CCAAGGGGAA GAATGTGATG ATATGAATAA GGTCAACGGG GATGGCTGCT 
     3481 CCCTTTTCTG CAAGCAAGAA GTTTCCTTCA ACTGCATTGA TGAACCCAGC CGGTGCTATT 
     3541 TCCATGATGG GGATGGGATG TGTGAAGAGT TTGAGCAAAA AACTAGCATT AAAGACTGTG 
     3601 GTGTCTACAC GCCCCAGGGT TTCCTGGATC AGTGGGCGTC CAATGCTTCA GTATCCCATC 
     3661 AAGACCAGCA GTGCCCAGGT TGGGTTGTCA TTGGGCAGCC GGCAGCCTCT CAGGTGTGTC 
     3721 GAACCAAGGT GATAGATCTC AGTGAAGGCA TTTCCCAGCA TGCTTGGTAT CCTTGCACCA 
     3781 TCAATTACCC ATACTATCAG CTGCCTCAGA CCACATTCTG GCTCCAGACA TATTTCTCTC 
     3841 AGCCAATGGT GGCTGCAGCT GTTATTATTC ACCTGGTGAC TGATGGGACA TACTATGGGG 
     3901 ACCAAAAGCA AGAGACCATC AGCCTCCATG TCTTGAGCTG CAGAAACAAT CCCCTGATTA 
     3961 TCCCTGTGGT CCATGACCTC AGCCAGCCCT TCTACCACAG CCAGGCGGTA CATGTGAGCT 
     4021 TCAGTTCGCC CCTGGTCGCC ATCTCGGGGG TGGCCCTCCG ATCCTTCGAC AACTTTGACC 
     4081 CCGTCACCCT GAGCAGCTGC CAGAGAGGAG AGACCTACAG CCCTGCTGAG CAGAGCTGTG 
     4141 TGCATTTTGC CTGTGAAGCC ACGGACTGCC CAGAACTGAC TGTGGAGAAT GCTTCTCTCA 
     4201 ACTGTTCCAG CAGCCATCGT TATCATGGTG CTCAGTGCAC CGTAAGCTGC CAGACAGGTT 
     4261 ATGTGCTGCA GATACAGCGA GATGATGAGC TAATCAAGAG CCAGGTAGGG CCAAGCGTCA 
     4321 CAGTGACATG TACAGAGGGC AAATGGAACA AGCAGGTGGC ATGTGAGCCT GTAGACTGCG 
     4381 GTATCCCGGA TCACCATCAC GTCTACGCCG CCTCCTTCTC CTGTCCAGAG GGCAACAACA 
     4441 GCTTTCTGAC CTGTATGGAA GATGGACTGT GGTCCTTCCC AGAGGCACTG TGTGAGCTTA 
     4501 TGTGCCTCGC CCCACCCCCA GTTCCCAATG CGGACCTACA GACAGCCCGG TGTCGCGAGA 
     4561 ACAAGCACAA GGTGGGCTCC TTATGCAAGT ACAAGTGCAA ACCTGGATAC CATGTGCCTG 
     4621 GCTCATCTCG GAAGTCCAAG AAACGGGCCT TCAAGACTCA ATGTACTCAA GACGGCAGCT 
     4681 GGCAAGAGGG AACATGTGTG CCGGTGACTT GTGACCCACC TCCACCCAAA TTCCATGGGC 
     4741 TCTATCAGTG CACTAATGGC TTCCAGTTCA ATAGTGAGTG TAGGATCAAG TGTGAAGACA 
     4801 GTGATGCCTC CCAGGGCCGT GGGAGCAACA CCATTCACTG CCGGAAAGAC GGCACTTGGA 
     4861 GTGGTTCCTT CCATGTCTGC CGAGAGATGC AAGGCCAGTG CTCAGCCCCA AACCAACTCA 
     4921 ACAGTCACCT CAAATTGCAG TGTCCTGACG GCTATGCCAT AGGGTCAGAG TGTGCCACCT 
     4981 CGTGCCTGGA CCACAACAGC GAGTCCATTA TCCTGCCTGT TAACTTGACG GTGCGTGACA 
     5041 TTCCCCATTG GATGAACCCC ACACGAGTAC AGAGGATTGT CTGCACTGCT GGTCTCCAGT 
     5101 GGTATCCCCA CCCTGCTCGG ATCCACTGTG TCAAAGGCTG TGAGCCATTC ATGGGAGACA 
     5161 ATTACTGTGA TGCCATCAAC AATCGAGCCT TCTGCAACTA TGATGGTGGG GACTGCTGCA 
     5221 CCTCCACAGT AAAGACCAAA AAGGTCACTC CCTTTCCTAT GTCCTGTGAC CTACAAGATG 
     5281 ACTGCGCCTG TCGGGACCCT GAGGCCCAAG AACACAGCCG GAAAGACCTT CGTGGATATA 
     5341 GCCATGGCAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//