LOCUS       RR202403                7277 bp    DNA     circular  SYN 
DEFINITION  Ppargc1a (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (Ppargc1a), (10 ug)
ACCESSION   NM_031347
VERSION     NM_031347.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3434..3525 
                     /label="Myc-DDK"  
     misc_feature    3555..4140 
                     /label="PolyA signal"  
     misc_feature    3612..3631 
                     /label="Restriction Enzyme"  
     misc_feature    4416..5088 
                     /label="ColE1"  
     misc_feature    5518..6312 
                     /label="Kan/Neo"  
     misc_feature    6347..6649 
                     /label="SV40 ori"  
     misc_feature    6735..7212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3416..3422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3416 
                     /label="Ppargc1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTGGGAC ATGTGCAGCC AAGACTCTGT ATGGAGTGAC ATAGAGTGTG 
     1081 CTGCCCTGGT TGGTGAGGAC CAGCCTCTTT GCCCAGATCT TCCTGAACTT GACCTTTCTG 
     1141 AACTTGATGT GAATGACCTG GACACAGACA GCTTTCTGGG TGGATTGAAG TGGTGTAGTG 
     1201 ACCAATCAGA AATCATATCC AACCAGTACA ACAATGAGCC CGCGAACATA TTCGAGAAGA 
     1261 TAGATGAAGA GAATGAGGCA AACTTGCTAG CGGTCCTCAC AGAGACACTG GACAGTCTCC 
     1321 CGGTGGATGA AGACGGATTG CCCTCATTTG ATGCACTGAC AGATGGAGAC GTGACCACTG 
     1381 ACAACGAGGC CAGTCCTTCC TCCATGCCTG ACGGCACCCC TCCCCCTCAG GAGGCAGAAG 
     1441 AGCCGTCTCT ACTTAAGAAG CTCTTACTGG CGCCAGCCAA CACTCAGCTC AGCTACAATG 
     1501 AATGCAGCGG TCTTAGCACT CAGAACCATG CAAACCACAC CCACAGGATC AGAACAAACC 
     1561 CTGCCATTGT TAAGACCGAG AATTCATGGA GCAATAAAGC AAAGAGCATT TGTCAACAGC 
     1621 AAAAGCCACA AAGACGTCCC TGCTCAGAGC TTCTCAAGTA TCTGACCACA AACGATGACC 
     1681 CTCCTCACAC CAAACCCACA GAGAACAGAA ACAGCAGCAG AGACAAATGT GCTTCCAAAA 
     1741 AGAAGTCCCA TACACAACCG CAGTCGCAAC ATGCTCAAGC CAAACCAACA ACTTTATCTC 
     1801 TTCCTCTGAC CCCAGAGTCA CCAAATGACC CCAAGGGTTC CCCATTTGAG AACAAGACTA 
     1861 TTGAGCGAAC CTTAAGTGTG GAACTCTCTG GAACTGCAGG CCTAACTCCT CCCACGACTC 
     1921 CTCCTCATAA AGCCAACCAA GATAACCCTT TCAAGGCTTC GCCAAAGCTG AAGCCCTCTT 
     1981 GCAAGACCGT GGTGCCACCA CCAACAAAGA GGGCCCGGTA CAGTGAGTGT TCTGGTACCC 
     2041 AAGGCAGCCA CTCCACCAAG AAAGGGCCGG AGCAATCTGA GTTATACGCA CAACTCAGCA 
     2101 AGTCCTCAGT GCTCAGCCGA GGACACGAGG AAAGGAAGAC TAAACGGCCC AGTCTACGGC 
     2161 TGTTTGGTGA TCATGACTAC TGTCAGTCAG TCAATTCCAA AACGGATATA CTCATTAACA 
     2221 TATCACAGGA GCTCCAAGAC TCTAGACAAC TAGACTTCAA AGATGCCTCC TGTGACTGGC 
     2281 AGGGGCACAT CTGTTCTTCC ACAGATTCAA GCCAGTGCTA CCTGAGAGAG ACTTTGGAGG 
     2341 CCAGCAAGCA GGTCTCTCCT TGCAGCACCA GAAAACAGCT CCAAGACCAG GAAATCCGAG 
     2401 CTGAGCTGAA CAAGCACTTC GGTCATCCCA GTCAAGCTGT TTTTGACGAC AAAGTAGACA 
     2461 AGACCAGTGA ACTACGGGAT GGCAACTTCA GTAATGAACA ATTCTCCAAA CTACCTGTGT 
     2521 TTATAAATTC AGGACTAGCC ATGGATGGCC TATTTGATGA CAGTGAAGAT GAAAATGATA 
     2581 AACTGAGCTA CCCTTGGGAT GGCACGCAGT CCTATTCATT GTTCGATGTG TCGCCTTCTT 
     2641 GCTCTTCCTT TAACTCTCCG TGTCGAGATT CAGTGTCACC ACCGAAATCC TTATTTTCTC 
     2701 AAAGACCCCA AAGGATGCGC TCTCGTTCGA GATCCTTTTC TCGACACAGG TCGTGTTCCC 
     2761 GATCACCATA TTCCAGGTCA AGATCAAGGT CCCCAGGCAG TAGATCCTCT TCAAGATCCT 
     2821 GTTACTATTA TGAATCAAGC CACTACAGAC ACCGCACACA TCGCAATTCT CCCTTGTATG 
     2881 TGAGATCACG TTCAAGGTCA CCCTACAGCC GTAGGCCCAG GTATGACAGC TATGAAGCCA 
     2941 ATGAGCACGA AAGGCTCAAG AGGGACGAAT ACCGCAGAGA GTATGAGAAG CGGGAGTCTG 
     3001 AAAGGGCCAA GCAGAGAGAG AGGCAGAAGC AGAAAGCAAT TGAAGAGCGC CGTGTGATTT 
     3061 ACGTTGGTAA AATCAGACCT GACACAACGC GGACAGAACT GAGAGACCGC TTTGAAGTTT 
     3121 TTGGTGAAAT TGAGGAATGC ACCGTAAATC TGCGGGATGA TGGAGACAGC TATGGTTTCA 
     3181 TTACCTACCG TTACACCTGT GACGCTTTCG CTGCTCTTGA GAATGGATAT ACTTTACGCA 
     3241 GGTCGAATGA AACTGACTTC GAGCTGTACT TTTGTGGACG GAAGCAATTT TTCAAGTCTA 
     3301 ACTATGCAGA CCTAGATTCA AACTCAGACG ATTTTGACCC TGCTTCCACC AAGAGCAAGT 
     3361 ATGACTCTCT GGATTTTGAT AGTTTACTGA AGGAAGCTCA GAGAAGCTTG CGCAGGTGCG 
     3421 CAGGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     3481 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG 
     3541 CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG 
     3601 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA 
     3661 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA 
     3721 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA 
     3781 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC 
     3841 TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT 
     3901 TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT 
     3961 CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG 
     4021 CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC 
     4081 AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG 
     4141 TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG 
     4201 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 
     4261 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     4321 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     4381 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     4441 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     4501 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     4561 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     4621 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     4681 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     4741 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     4801 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     4861 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     4921 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     4981 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 
     5041 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC 
     5101 GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA 
     5161 AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC 
     5221 CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC 
     5281 TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA 
     5341 GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC 
     5401 ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG 
     5461 GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA 
     5521 GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG 
     5581 CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA 
     5641 TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT 
     5701 CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT 
     5761 GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG 
     5821 CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA 
     5881 TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG 
     5941 GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG 
     6001 CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC 
     6061 CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG 
     6121 ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA 
     6181 AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG 
     6241 TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT 
     6301 GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG 
     6361 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG 
     6421 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG 
     6481 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC 
     6541 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC 
     6601 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 
     6661 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT 
     6721 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     6781 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     6841 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     6901 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     6961 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     7021 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     7081 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     7141 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     7201 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     7261 AATTTAACGC GAATTTT
//