LOCUS       RR202350                6035 bp    DNA     circular  SYN 
DEFINITION  Galt (Myc-DDK-tagged ORF) - Rat galactose-1-phosphate uridylyltransferase (Galt), (10 ug)
ACCESSION   NM_001013089
VERSION     NM_001013089.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2192..2283 
                     /label="Myc-DDK"  
     misc_feature    2313..2898 
                     /label="PolyA signal"  
     misc_feature    2370..2389 
                     /label="Restriction Enzyme"  
     misc_feature    3174..3846 
                     /label="ColE1"  
     misc_feature    4276..5070 
                     /label="Kan/Neo"  
     misc_feature    5105..5407 
                     /label="SV40 ori"  
     misc_feature    5493..5970 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2174..2180 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2174 
                     /label="Galt"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGCAAAGC GGAGCCGATC CTGAGCAGCG CCAGCAGGCT TCAGAGGCGG 
     1081 ACGCCATGGC AGCGACCTTC CGGGCGAGCG AACACCAGCA TATTCGCTAC AACCCGCTCC 
     1141 AGGATGAGTG GGTGTTAGTG TCAGCCCATC GCATGAAGCG GCCCTGGCAA GGACAAGTGG 
     1201 AGCCCCAGCT TCTGAAGACG GTCCCCCGCC ATGACCCACT CAACCCTCTG TGTCCCGGGG 
     1261 CCACACGAGC TAATGGGGAG GTGAATCCCC CCTATGATGG CACCTTCTTA TTTGACAATG 
     1321 ACTTCCCGGC TCTGCAGCCC GATGCTCCGG ATCCAGGACC CAGTGACCAC CCTCTCTTCC 
     1381 GAGTGGAGGC CGCCAGAGGA GTTTGTAAGG TCATGTGCTT CCACCCCTGG TCGGATGTGA 
     1441 CGCTGCCACT CATGTCTGTC CCTGAGATCC GAGCTGTCAT CGATGCATGG GCCTCAGTCA 
     1501 CAGAGGAACT GGGCGCCCAG TACCCTTGGG TGCAGATCTT TGAAAACAAA GGAGCCATGA 
     1561 TGGGTTGCTC TAACCCCCAT CCCCACTGCC AGGTTTGGGC TAGCAATTTC CTGCCAGATA 
     1621 TTGCCCAGCG TGAAGAGCGA TCCCAGCAGA CTTATCACAA CCAGCATGGA AAGCCTTTAT 
     1681 TATTGGAATA TGGCCACCAA GAGCTCCTCA GGAAGGAACG TCTGGTCCTA ACCAGTGAGT 
     1741 ACTGGATAGT TCTGGTCCCC TTCTGGGCAG TGTGGCCTTT CCAGACATTA CTGCTGCCCC 
     1801 GACGGCACGT GCAGCGGCTA CCTGAGCTGA CCCCTGCTGA GCGTGATGAT CTAGCCTCCA 
     1861 CCATGAAGAA GCTCTTGACC AAGTATGACA ACCTGTTTGA GACGTCCTTT CCTTACTCCA 
     1921 TGGGCTGGCA CGGGGCTCCC ATGGGATTAA AGACTGGAGC CACCTGTGAC CACTGGCAGC 
     1981 TCCATGCTCA CTATTACCCC CCACTCCTGC GATCGGCTAC TGTTCGGAAA TTCATGGTTG 
     2041 GCTATGAAAT GCTTGCCCAG GCCCAGCGTG ACCTCACTCC CGAACAGGCT GCAGAAAGAT 
     2101 TAAGGGTGCT CCCTGAGGTA CACTATTGCC TGACGCAGAA AGACAAGGAA ACAGCAGCCA 
     2161 CCGCTAAACA GCAGCCACCG CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     2221 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC 
     2281 GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT 
     2341 CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA 
     2401 TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA 
     2461 GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT 
     2521 ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT 
     2581 GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA 
     2641 CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC 
     2701 TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT 
     2761 TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC 
     2821 AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA 
     2881 GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT 
     2941 TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 
     3001 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 
     3061 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 
     3121 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 
     3181 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 
     3241 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 
     3301 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 
     3361 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 
     3421 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 
     3481 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 
     3541 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 
     3601 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 
     3661 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 
     3721 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 
     3781 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG 
     3841 GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT 
     3901 GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT 
     3961 GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA 
     4021 ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT 
     4081 GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA 
     4141 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT 
     4201 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG 
     4261 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA 
     4321 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC 
     4381 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA 
     4441 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG 
     4501 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA 
     4561 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT 
     4621 CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT 
     4681 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG 
     4741 ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA 
     4801 ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC 
     4861 CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG 
     4921 ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG 
     4981 CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG 
     5041 CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG 
     5101 TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG 
     5161 AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA 
     5221 TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC 
     5281 GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG 
     5341 CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     5401 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC 
     5461 TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     5521 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     5581 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     5641 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     5701 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     5761 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     5821 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     5881 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     5941 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     6001 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//