LOCUS       RR202319                8796 bp    DNA     circular  SYN 
DEFINITION  Robo3 (Myc-DDK-tagged ORF) - Rat roundabout homolog 3 (Drosophila) (Robo3), (10 ug)
ACCESSION   NM_001108135
VERSION     NM_001108135.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4953..5044 
                     /label="Myc-DDK"  
     misc_feature    5074..5659 
                     /label="PolyA signal"  
     misc_feature    5131..5150 
                     /label="Restriction Enzyme"  
     misc_feature    5935..6607 
                     /label="ColE1"  
     misc_feature    7037..7831 
                     /label="Kan/Neo"  
     misc_feature    7866..8168 
                     /label="SV40 ori"  
     misc_feature    8254..8731 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4935..4941 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4935 
                     /label="Robo3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACGCATC GTGGAGCAGC CGCCAGATCT GCTCGTTTCC AGGGGCGAGC 
     1081 CGGCCACTCT GCCCTGCCGT GCTGAAGGCC GGCCTCGACC CAACATCGAG TGGTACAAGA 
     1141 ATGGGGCGCG CGTGGCCACT GCACGCGAGG ATCCGCGGGC TCACCGCCTG CTGCTGCCCA 
     1201 GCGGCGCCCT CTTCTTTCCC CGCATCGTGC ACGGGCGTCG CTCTCGGCCT GACGAGGGTG 
     1261 TCTACACCTG TGTGGCTCGC AACTACCTGG GAGCAGCGGC TAGCAGAAAC GCCTCTCTGG 
     1321 AAGTAGCTGT CCTCCGAGAC GATTTCCGGC AATCTCCTGG GAACGTGGTG GTAGCAGTGG 
     1381 GGGAGCCAGC AGTAATGGAA TGCGTGCCCC CTAAGGGTCA CCCAGAGCCT TTGGTGACCT 
     1441 GGAAGAAGGG CAATACAAAG CTTAAGGAAG AGGAGGGAAG GATCACCATA CGTGGAGGAA 
     1501 AGCTGATGAT GTCACATACA TTCAAGAGTG ATGCTGGCAT GTACATGTGT GTAGCCTCCA 
     1561 ACATGGCTGG AGAACGAGAG AGTGGGGCAG CTGAACTTGT GGTACTGGAG CGTCCCTCAT 
     1621 TTCTGCGTAG ACCAATAAAC CAGGTTGTCC TGGCTGATGC CCCTGTGAAT TTCCTGTGTG 
     1681 AGGTGCAGGG AGATCCCCAG CCCAATCTAC GCTGGCGCAA GGATGATGGG GAGCTGCCCA 
     1741 CTGGCAGGTA TGAGATTCGG AGTGACCACA GCCTTTGGAT CGAGCAAGTG AGTGCTGAAG 
     1801 ACGAGGGGAC TTACACCTGT GTGGCAGAAA ACAGTGTGGG CCGAGCAGAG GCATCTGGCT 
     1861 CCCTCAGTGT TCACGTCCCA CCACAGTTTG TGACTAAGCC CCAGAACCAG ACAGCAGCTC 
     1921 CTGGAGAAAA CGTGTCTTTC CAATGTGAGA CCAAAGGAAA CCCACCACCT GCCATCTTCT 
     1981 GGCAGAAGGA AGGGAGTCAA GTCTTGCTTT TCCCTAGTCA GTCACTCCAG CCCATGGGAC 
     2041 GCCTCTTAGT CTCTCCAAGA GGCCAGCTCA ACATCACTGA AGTAAAGATC GGGGATGGTG 
     2101 GCTACTATGT GTGCCAGGCT GTCAGTGTGG CTGGTAGCAT CCTGGCTAAG GCCCTGTTAG 
     2161 AGATAAAAGG AGCCTCCATA GATGGACTAC CTCCCATCAT TCTCCAGGGA CCAGCCAATC 
     2221 AGACATTGGT ACTTGGCTCT TCTGTGTGGC TGCCATGCAG AGTGATTGGA AACCCTCAGC 
     2281 CCAATGTCCA ATGGAAGAAG GATGAGACGT GGCTGCAGGG GGATAACTCA CAGTTCAACT 
     2341 TAATGGACAA CGGCACACTA TACATTGCCA GTGTACAGGA GATGGACATG GGTTTCTACA 
     2401 GCTGTGTGGC CAAGAGTTCC ATAGGGGAGG CCACATGGAA TAGCTGGCTT AGGAAGCGAG 
     2461 AACATTGGGG AGCATCACCA GGTCCAGCAA CAGAACCCGG TAATCCTCCA GGCCCTCCCT 
     2521 CTCAGCCAAT AGTCACAGAG GTAACCACAA ACAGCATTAC CTTGACCTGG AAGCCCAATC 
     2581 CACAGTCTGG GGCCAGAGCT ACCTCCTATG TGATAGAGGC CTTCAGCCAA GCAGCTGGCA 
     2641 ACACGTGGCG GACGGTGGCA GATGGGGTAC AGCTGGAAAC GCACACCGTC AGCGGCCTGC 
     2701 AGCCCAATAC CATCTACCTA TTCCTCGTGA GAGCTGTTGG AGCCTGGGGC CTCAGTGAAC 
     2761 CCAGTCCTGT CTCTGAGCCT GTTCAAACCC AGGACAGCAG CCTATCTAGG CCAGTGGAGG 
     2821 ACCCGTGGAA AGGCCAGCAA GGACTGGCTG AAGTGGCCGT GCGAATGCAG GAGCCCATAG 
     2881 TCCTTGGACC CAGAACTCTG CAGGTGTCCT GGACTGTAGA GGGCCCTGGC CAGCTGGTTC 
     2941 AGGGATTCCG TGTGTCTTGG AGGATTGCAG GCCTTGACCA GGGAAGCTGG ACAGCGCTAG 
     3001 ACGTACAGAG TCCACACAAG CAAAGTACTG TGCTAAGAGG CCTCCCCCCA GGGTCCCAAA 
     3061 TACAGATCAA GGTGCAAGTC CAAGGCCAGG AGGGGCTGGG GGCTGAAAGC CCCTCTGTGA 
     3121 CCCGGAGCAT TCCTGAAGAG GCCCCCAGTG GCCCCCCTCA GGGAGTGGCT GTGGCCTTGG 
     3181 GGGGTGATCG CAACAGCAGT ATCACTGTAT CCTGGGAGCC TCCACTTCCC TCCCAGCAAA 
     3241 ATGGGGTTAT CACTGAATAC CAGATCTGGT GCCTGGGTAA TGAAAGCCGA TTCCACCTCA 
     3301 ATCGATCTGC AGCAGGCTGG GCACGCTCTG TGATGTTCCG GGGACTGCTA CCGGGCCTGC 
     3361 TCTATAGAGC CATAGTGGCA GCAGCTACCA GCGCTGGCGT GGGCGTGGCC AGTGCCCCAG 
     3421 TGCTAGTGCA GCTGCCATTC CCTCCGGTTG CGGAGCCCGG GCCTGAGGTC AGCGAGGGGC 
     3481 TGGCGGAACG GTTGTCTAGG GTGCTGCGGA AGCCTGCTTT CCTGGCTGGC AGCAGCGCGG 
     3541 CCTGTGGGGC GCTGCTTCTC GGGCTCTGCG CCGCCCTCTA TCGGCGCCAG AAGCAACGCA 
     3601 AAGAGCTCAG TCATTATACC GCCTCCTTTG CCTACACACC TGCAGTGTCC TTTCCACACT 
     3661 CAGAGGGCCT TTCTGGATCC AGTTCCAGGC CACCCATGGG CCTTGGCCCT GCTGCCTACC 
     3721 CATGGCTGGC AGACTCCTGG CCCCACCCAT CTCGAAGTCC CTCAGCTCAG GAACCCCGGG 
     3781 GGAGCTGCTG CCCCAGCAAT CCTGATCCAG ATGATAGATA CTACAATGAG GCAGGAATCT 
     3841 CCCTGTACTT GGCTCAGACT GCACGGGGTG CTAATGCCCC TGGCGAGGGT CCTGTCTACA 
     3901 GCACCATTGA CCCAGTAGGG GAAGAGCTAC AGACCTTCCA CGGAGGGTTC CCTCAACATT 
     3961 CTTCTGGGGA CCCAAGCACC TGGAGCCAGT ATGCTCCCCC CGAATGGAGC GAGGGGGACA 
     4021 GTGGAGCCAG GGGAGGCAAA GGGAAGCTTC TAGGCAAACC TGTGCAGATG CCCTCTTTGA 
     4081 GCTGGCCAGA AGCCCTGCCG CCACCTCCCC CTTCCTGTGA ATTGAGCTGC CCAGAGGGGC 
     4141 CTGAGGAGGA GCTGAAGGGC AGTTCAGATC TGGAAGAGTG GTGCCCACCA ATGCCTGAGA 
     4201 AAAAACACTT GATTGACTCA CGTTCCAGTG GTGCGTGCAT GGTGGCTCCA GCCCGAGGGG 
     4261 AAACTCCCTC CCCTACCTCT TCTTACGGAC AGCAGTCCAC AGCCACTCTT ACCCCCTCAC 
     4321 CTCCTGACCC TCCCCAGCCC CCTAGCGACA TCCCACATCT CCATCAGATG CCCAGGAGGG 
     4381 TGCCCCTTGG ACCAAGTTCT CCTCTCAGCG TATCCCAGCC TGCTCTGAGT AGCCATGATG 
     4441 GAAGGCCTGT TGGCCTGGGT GCTGGCCCTA TAGTCTCTTA TCACCCTAGC CCCAGTCCTG 
     4501 TCCCTAGTAC AGCCAGCAGT GCACCAGGGA GAACTCGCCA GGTAACTGGA GAGATGACTC 
     4561 CCCCACTCCA TGGACACCGT GCCCGAATCC GGAAGAAAAC CAAGGCTCTT CCACATAGAA 
     4621 GGGAACACAG TCCTGGGGAC TTGCCCCCAC CACCCTTGCC ACCACCAGAG GAAGAGACGA 
     4681 GCTGGCCCTT GGGTCTGAGA GCAGCAGGCA GCATGTCTTC CCTAGAGCGA GAGCACAGTG 
     4741 GGGAGAGGAG AGTGGCCCAG GCAGTGCCTC TAGGGGCCCA ACCTCCAGGG GCCCAACGTG 
     4801 GCCCCCACCC TGATGGTAAG GAGAGCCAGG GCAATGGGAG GGGCCTGGAG GCTTGCAGAA 
     4861 GTCCCCAAAG TCCCCAGATT CAGTTGGATT CACGTGTCTG GTCAACATTG ATATTGTCTC 
     4921 TTATCTCTTT CACTTCCTCT AAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//