LOCUS       RR202293                8619 bp    DNA     circular  SYN 
DEFINITION  Kcnma1 (Myc-DDK-tagged ORF) - Rat potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), (10 ug)
ACCESSION   NM_031828
VERSION     NM_031828.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4774..4865 
                     /label="Myc-DDK"  
     misc_feature    4895..5480 
                     /label="PolyA signal"  
     misc_feature    4952..4971 
                     /label="Restriction Enzyme"  
     misc_feature    5756..6428 
                     /label="ColE1"  
     misc_feature    6858..7652 
                     /label="Kan/Neo"  
     misc_feature    7687..7989 
                     /label="SV40 ori"  
     misc_feature    8075..8552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="Kcnma1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACGGT GGCGGCGGCG GCGGCGGCGG CAGCAGCGGC AGCAGCGGCG 
     1081 GCGGCGGCGG CGGCGGCGGA GGCGAGACGG CTCTTAGAAT GAGCAGCAAT ATCCACGCGA 
     1141 ACCATCTCAG CCTAGACGCG TCCTCTTCTT CCTCCTCCTC TTCTTCCTCC TCCTCTTCCT 
     1201 CCTCGTCCTC GGTCCACGAG CCCAAGATGG ATGCGCTCAT CATCCCGGTG ACCATGGAGG 
     1261 TGCCGTGCGA CAGCCGGGGC CAACGCATGT GGTGGGCTTT CTTGGCCTCC TCCATGGTGA 
     1321 CTTTCTTCGG GGGCCTCTTC ATCATCTTGC TCTGGCGGAC TCTCAAGTAC CTGTGGACCG 
     1381 TTTGCTGCCA CTGCGGGGGC AAGACGAAGG AGGCCCAGAA GATAAACAAT GGCTCCAGCC 
     1441 AGGCAGATGG TACTCTCAAG CCAGTGGATG AAAAAGAGGA GGTGGTGGCA GCCGAGGTCG 
     1501 GCTGGATGAC ATCTGTGAAG GACTGGGCAG GGGTGATGAT ATCTGCCCAG ACACTGACTG 
     1561 GCAGAGTCCT GGTTGTGTTA GTCTTTGCTC TCAGCATTGG TGCCCTTGTA ATATACTTCA 
     1621 TAGACTCATC AAACCCAATA GAATCCTGCC AGAATTTCTA CAAAGATTTC ACATTACAGA 
     1681 TCGACATGGC TTTCAACGTG TTCTTCCTTC TCTACTTTGG CTTGCGGTTT ATTGCAGCCA 
     1741 ATGATAAGCT GTGGTTCTGG CTGGAAGTGA ATTCTGTAGT AGATTTCTTC ACAGTCCCTC 
     1801 CCGTGTTTGT GTCTGTATAC TTAAACAGAA GTTGGCTTGG CTTGAGATTT TTAAGAGCTC 
     1861 TCAGACTGAT TCAGTTTTCA GAAATTTTGC AGTTTCTGAA TATCCTTAAA ACAAGTAATT 
     1921 CCATCAAGCT GGTGAACCTG CTCTCCATAT TTATCAGCAC GTGGCTGACT GCAGCTGGAT 
     1981 TCATCCACTT GGTGGAGAAT TCAGGGGACC CATGGGAAAA TTTCCAAAAC AACCAGGCAC 
     2041 TTACGTACTG GGAATGTGTC TACTTACTCA TGGTCACAAT GTCTACAGTG GGTTACGGGG 
     2101 ATGTTTATGC AAAAACCACG CTTGGACGCC TCTTCATGGT CTTCTTCATC CTCGGGGGAC 
     2161 TGGCCATGTT TGCCAGCTAC GTCCCTGAAA TCATAGAGTT AATAGGAAAC CGCAAGAAAT 
     2221 ACGGGGGCTC CTATAGCGCG GTTAGTGGAA GAAAGCACAT TGTAGTCTGT GGGCACATTA 
     2281 CTCTGGAGAG TGTCTCTAAC TTCCTGAAGG ACTTTCTGCA CAAGGACCGG GATGATGTCA 
     2341 ACGTGGAGAT TGTTTTTCTT CACAACATCT CCCCTAACCT TGAGCTTGAG GCTCTGTTCA 
     2401 AACGGCATTT CACTCAGGTG GAGTTTTATC AGGGCTCTGT CCTCAATCCA CACGATCTTG 
     2461 CCAGAGTCAA GATAGAGTCA GCAGATGCAT GTTTGATCCT TGCCAATAAG TACTGCGCTG 
     2521 ACCCGGATGC AGAAGATGCC TCCAACATCA TGAGAGTGAT CTCCATAAAG AACTACCACC 
     2581 CAAAGATCAG GATCATCACT CAGATGCTGC AGTATCACAA CAAGGCCCAT CTGCTCAACA 
     2641 TCCCCAGCTG GAACTGGAAA GAGGGTGATG ACGCAATATG CCTTGCAGAG CTCAAGTTGG 
     2701 GTTTCATAGC CCAGAGCTGT CTGGCTCAAG GCCTCTCCAC AATGCTTGCC AATCTCTTCT 
     2761 CTATGAGGTC ATTCATAAAG ATTGAGGAAG ATACATGGCA GAAATACTAC TTGGAAGGAG 
     2821 TCTCCAATGA AATGTACACA GAATATCTTT CCAGTGCCTT CGTGGGTCTG TCCTTCCCTA 
     2881 CTGTTTGTGA GCTGTGTTTT GTGAAGCTTA AGCTCCTGAT GATAGCCATT GAGTACAAGT 
     2941 CTGCCAACAG GGAGAGCCGA AGCCGAAAGC GAATATTAAT TAACCCTGGG AACCACCTTA 
     3001 AGACCCAAGA AGGTACTTTA GGATTTTTCA TCGCAAGTGA TGCCAAAGAA GTTAAAAGGG 
     3061 CATTTTTTTA CTGCAAGGCC TGTCATGATG ACGTCACAGA TCCCAAAAGA ATTAAAAAAT 
     3121 GCGGCTGCAG GCGGCTGATC TATTCCAAGA TGTCCATCTA CAAGAGAATG AGCCGAGCAT 
     3181 GTTGTTTTGA TTGCGGACGT TCTGAGCGTG ACTGCTCGTG CATGTCAGGC CGTGTGCGTG 
     3241 GTAACGTGGA CACCCTTGAG AGAAACTTCC CGCTTTCTTC TGTCTCTGTT AATGATTGCT 
     3301 CCACCAGTTT CCGTGCCTTT GAAGATGAGC AACCGCCAAC ACTGTCACCA AAAAAAAAAC 
     3361 AACGTAATGG GGGCATGAGG AACTCACCCA ACACATCCCC GAAGCTGATG AGGCATGACC 
     3421 CCTTGTTAAT TCCTGGCAAC GATCAGATTG ACAACATGGA CTCCAATGTG AAAAAGTACG 
     3481 ACTCCACTGG AATGTTTCAC TGGTGCGCAC CCAAGGAGAT TGAGAAAGTC ATCTTGACTC 
     3541 GAAGTGAAGC TGCCATGACT GTCCTGAGTG GTCATGTTGT AGTCTGCATC TTTGGGGATG 
     3601 TCAGCTCAGC CCTGATTGGC CTCCGGAACC TGGTGATGCC GCTTCGTGCT AGCAATTTTC 
     3661 ACTATCATGA GCTCAAACAC ATTGTGTTTG TGGGCTCCAT CGAGTACCTC AAGCGGGAGT 
     3721 GGGAAACACT GCACAACTTC CCCAAAGTAT CCATATTGCC TGGTACGCCA TTAAGTCGGG 
     3781 CTGATTTAAG GGCTGTCAAC ATCAACCTCT GTGACATGTG CGTTATCCTG TCAGCCAATC 
     3841 AGAATAATAT TGATGATACT TCGCTTCAGG ACAAGGAATG CATCTTGGCG TCACTCAACA 
     3901 TCAAATCTAT GCAGTTTGAT GACAGCATCG GGGTCTTGCA GGCTAATTCC CAAGGATTCA 
     3961 CACCTCCTGG AATGGACAGA TCATCACCTG ACAACAGCCC AGTGCACGGG ATGTTACGCC 
     4021 AGCCGTCCAT CACAACCGGA GTCAACATTC CCATCATCAC GGAACTCGTG AACGATACTA 
     4081 ATGTTCAGTT TTTGGACCAA GATGATGATG ATGACCCTGA CACAGAGCTG TACCTCACAC 
     4141 AGCCCTTTGC TTGTGGGACA GCATTTGCCG TTAGCGTCCT GGACTCACTC ATGAGCGCGA 
     4201 CATACTTCAA TGACAATATC CTCACCCTAA TACGGACCCT GGTGACAGGA GGAGCCACGC 
     4261 CAGAGCTCGA AGCTTTAATA GCTGAGGAGA ATGCACTTCG AGGGGGTTAC AGCACTCCGC 
     4321 AGACACTAGC CAACAGGGAC CGCTGCCGAG TGGCCCAGTT AGCCCTGTTG GATGGGCCCT 
     4381 TTGCGGACTT AGGGGATGGT GGTTGTTATG GTGATCTGTT CTGCAAAGCT CTGAAAACAT 
     4441 ATAACATGCT TTGTTTTGGA ATTTACCGGC TGAGAGATGC CCACCTCAGC ACCCCCAGCC 
     4501 AGTGTACAAA AAGGTACGTC ATCACCAATC CCCCCTACGA GTTTGAACTT GTACCGACAG 
     4561 ACCTGATCTT CTGCCTGATG CAGTTTGACC ACAACGCTGG CCAATCCCGG GCCAGTCTGT 
     4621 CTCATTCCTC CCACTCCTCA CAGTCGTCCA GTAAGAAGAG CTCCTCTGTC CACTCCATCC 
     4681 CGTCCACAGC AAATCGGCCA AACCGGCCCA AGTCCAGGGA GTCCCGCGAC AAACAGAAAA 
     4741 AAGAAATGGT TTACAGAAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4801 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4861 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4921 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4981 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5041 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5101 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5161 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5221 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5281 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5341 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5401 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5461 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5521 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5581 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5641 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5701 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5761 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5821 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5881 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5941 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6001 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6061 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6121 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6181 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6241 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6301 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6361 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6421 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6481 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6541 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6601 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6661 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6721 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6781 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6841 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6901 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6961 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7021 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7081 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7141 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7201 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7261 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7321 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7381 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7441 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7501 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7561 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7621 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7681 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7741 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7801 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7861 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8041 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8101 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8161 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8221 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8281 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8341 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8401 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8461 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8521 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8581 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//