LOCUS       RR202286                6319 bp    DNA     circular  SYN 
DEFINITION  Ptdss1 (Myc-DDK-tagged ORF) - Rat phosphatidylserine synthase 1 (Ptdss1), (10 ug)
ACCESSION   NM_001012113
VERSION     NM_001012113.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2476..2567 
                     /label="Myc-DDK"  
     misc_feature    2597..3182 
                     /label="PolyA signal"  
     misc_feature    2654..2673 
                     /label="Restriction Enzyme"  
     misc_feature    3458..4130 
                     /label="ColE1"  
     misc_feature    4560..5354 
                     /label="Kan/Neo"  
     misc_feature    5389..5691 
                     /label="SV40 ori"  
     misc_feature    5777..6254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2458..2464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2458 
                     /label="Ptdss1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCCTGC GTAGGGAGCA GGACTCTGAG CAAGGATGAT GTGAACTACA 
     1081 GGATGCATTT CCGCATGATC AACGAGCAGC AAGTGGAGGA CATCACCATA GACTTCTTCT 
     1141 ACCGGCCGCA CACCATCACC CTGCTCAGCT TTACCATCAT CAGCCTCATG TACTTCGCCT 
     1201 TCACCAGGGA TGACTGTGTT CCTGAAGACA ACATCTGGAG AGGCATCCTT TCCGTTATCT 
     1261 TCTTCTTCCT CATCATCAGC GTGTTAGCAT TCCCCAATGG CCCTTTTACT CGGCCCCATC 
     1321 CAGCCTTATG GCGAATGGTT TTTGGCCTCA GTGTGCTGTA CTTCCTCTTC TTGGTATTCC 
     1381 TCCTGTTCCT GAACTTCGAG CAGGTTAAAT CCCTGATGTA TTGGCTCGAC CCCAATCTCC 
     1441 GATACGCCAC CAGAGAAGCA GACATCATGG AGTATGCCGT GAACTGTCAT GTGATCACCT 
     1501 GGGAGAGAAT CGTTAGTCAC TTTGATATAT TTGCTTTTGG GCATTTCTGG GGCTGGGCCA 
     1561 TGAAGGCCTT GTTGATCCGT AGTTATGGGC TCTGCTGGAC AATCAGTATC ACCTGGGAGC 
     1621 TGACAGAGCT TTTCTTCATG CATCTTCTGC CCAACTTTGC TGAGTGCTGG TGGGACCAGG 
     1681 TCATTCTGGA CATCCTGTTG TGTAATGGTG GTGGAATCTG GCTGGGCATG GTCGTTTGCC 
     1741 GGTTTTTGGA GATGAGGACT TACCACTGGG CAAGCTTCAA GGACATCCAC ACCACCACAG 
     1801 GGAAGATCAA GCGTGCTGTG CTACAGTTCA CCCCTGCTAG CTGGACCTAC GTCAGGTGGT 
     1861 TTGACCCCAA GTCTTCCTTT CAGAGAGTGG CTGGTATATA CCTTTTCATG ATCATCTGGC 
     1921 AGCTGACTGA GTTGAATACC TTCTTCTTGA AGCACATCTT TGTGTTCCAA GCCAGCCATC 
     1981 CCTTAAGCTG GTGCAGAATT CTCTTCATCG GCTGCATTAC GGCTCCCACA GTGAGACAGT 
     2041 ACTACGCCTA CCTCACGGAC ACACAGTGCA AGCGTGTGGG GACACAGTGC TGGGTATTTG 
     2101 GGGTCATTGG TTTCCTGGAA GCCATTGTTT GCATCAAATT TGGACAAGAT CTCTTCTCTA 
     2161 AGACCCAGAT ACTCTACGTG GTGCTTTGGC TTCTTTGTGT GGCCTTCACT ACGTTCCTGT 
     2221 GTCTGTACGG CATGGTTTGG TATGCAGAGC ACTATGGACA CCGGGAAAAG ACCTATTCAG 
     2281 AGTGCGAAGA TGGCACTTAC AGTCCAGAGA TCTCTTGGTA TCACGGGAAA GGCTCAAAAG 
     2341 GTTCTGAGGA CAGCCCACCG AAGCATTCAA ACAACAACGA AAGCCATTCT TCCAGAAGAA 
     2401 GGAACCGGCA TTCCAAGTCC AAAGTCACCA ATGGAGTTGG GAAGAAACAA TGGAGTTGGG 
     2461 AAGAAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA 
     2521 AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT 
     2581 AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT 
     2641 GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT 
     2701 CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG 
     2761 CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG 
     2821 GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT 
     2881 CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT 
     2941 TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA 
     3001 ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC 
     3061 TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC 
     3121 ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC 
     3181 TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC 
     3241 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     3301 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     3361 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     3421 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     3481 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     3541 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     3601 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     3661 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     3721 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     3781 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     3841 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     3901 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     3961 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     4021 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     4081 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG 
     4141 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG 
     4201 AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT 
     4261 GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT 
     4321 TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT 
     4381 CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA 
     4441 TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG 
     4501 TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA 
     4561 GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG 
     4621 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC 
     4681 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA 
     4741 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC 
     4801 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC 
     4861 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC 
     4921 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC 
     4981 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG 
     5041 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT 
     5101 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA 
     5161 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC 
     5221 AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT 
     5281 TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC 
     5341 GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT 
     5401 TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG 
     5461 AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG 
     5521 CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG 
     5581 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     5641 ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     5701 GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT 
     5761 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 
     5821 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 
     5881 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 
     5941 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 
     6001 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 
     6061 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 
     6121 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 
     6181 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 
     6241 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 
     6301 AAAATTTAAC GCGAATTTT
//