LOCUS       RR202265                6181 bp    DNA     circular  SYN 
DEFINITION  Actl9 (Myc-DDK-tagged ORF) - Rat similar to actin-like 7-alpha-like protein (RGD1306886), (10 ug)
ACCESSION   NM_001108077
VERSION     NM_001108077.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2338..2429 
                     /label="Myc-DDK"  
     misc_feature    2459..3044 
                     /label="PolyA signal"  
     misc_feature    2516..2535 
                     /label="Restriction Enzyme"  
     misc_feature    3320..3992 
                     /label="ColE1"  
     misc_feature    4422..5216 
                     /label="Kan/Neo"  
     misc_feature    5251..5553 
                     /label="SV40 ori"  
     misc_feature    5639..6116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2320..2326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2320 
                     /label="Actl9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTCAAT GGATACCCAA AGCCCCAGCC CTCCCCCGAA ACTCATGGGC 
     1081 CTCCTTCCCT GACCTCTAAC ACAGTCCTGG TGAGCAAGAA TCTAGAGCAA GATTCCTTCA 
     1141 GTGTGGTGGG AGACAGGTTC CCACTGAAAA CAGGTGCTGT GGTTATTGAC ATGGGCACAG 
     1201 GCACCTGTAA GGTAGGCTTT GCAGGACAAT CCCAACCCAC CTACTCGGTG GCCACCATCC 
     1261 TGGGTTGCCA GCCCAAGAAA CAGGCTACCA AGGGCCAATC GGAGCTGGAA ACTTTTATTG 
     1321 GGGAGGCAGC CCGCTCCCGC CCAGAACTGA GGTTGGTGAA GCCTATTCGC AATGGCATTG 
     1381 TGGTGGACTG GGAAGCAGCC GAACTAATAT GGCGACACAT CCTGGAACAT GATCTCCGAG 
     1441 TAGCCACCCA AGAGCACCCT CTACTGTTTT CGGATCCACC CTTCAGCCCT GCCACCAACC 
     1501 GAGAGAAGCT AGTAGAGGTG GCCTTTGAGT CTCTGCACTC CCCAGCCTTG TATGTGGCCT 
     1561 CTCAATCTGT ATTGTCAGTC TATGCTCACG GACGTGTTAA CGGACTCGTT GTGGACACTG 
     1621 GCCACGGAGT CAGCTACACA GTGCCAGTTG TTCAGGGTTA CAACTTACCC CATGCCATCC 
     1681 AACGCTTGGA CCTGGCAGGC AACCATCTTA CTGCCTTCCT AGCAGAAATG ATCCTGGGCT 
     1741 CTGGCTTCTC ATTGCAACAG GAGGACCTGG ACTTGGTGGA GAACATCAAA CATCATTACT 
     1801 GCTATTTGGC CTCAGATTTC CAAAAGGAAC AGGCCCGCCC AGATCAGGAG TGCAAGCAAA 
     1861 CTCTGAGGCT TCCTGATGGA CGGACTGTTA CTCTGGGAAA AGAGTTATTC CAGTGTCCAG 
     1921 AGCTCCTATT CCATCCCCCA GAGATTCCAG GATTATCGCC CATGGGCCTC CCAGCCATGG 
     1981 CTGAACAGAG CCTCCTCAGG GTGCCTCAAG ATCTACGGCC CCATGTGGCC CAGAATGTGA 
     2041 TCTTATGTGG AGGCTCCTCA CTCTTCACCG GCCTGGAGAG TCGCTTCAGG GCAGAGCTGC 
     2101 TACGTTCTCT GTCCCCTGAG GACCACGTAG TGGTCATGGC ACAGCCCAAC AGGAATCTTT 
     2161 CAGTGTGGAT CGGGGGCTCC ATTTTGGCCT CACTACATGC TTTCCAGTCC TGTTGGGTCC 
     2221 TGCGGGAACA ATATGAAGAA ATGGGACCCC AGGTTGTATA CCGAAAATGC TACCCTGCGG 
     2281 GAACAATATG AAGAAATGGG ACCCCAGGTT GTATACCGAA AATGCTACAC GCGTACGCGG 
     2341 CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC 
     2401 AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA 
     2461 TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC 
     2521 TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT 
     2581 GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA 
     2641 GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC 
     2701 TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA 
     2761 GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG 
     2821 ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC 
     2881 ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT 
     2941 TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG 
     3001 CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG 
     3061 GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC 
     3121 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     3181 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     3241 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     3301 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     3361 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     3421 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     3481 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     3541 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     3601 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     3661 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     3721 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 
     3781 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     3841 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     3901 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     3961 TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG 
     4021 CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG 
     4081 CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA 
     4141 CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG 
     4201 TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG 
     4261 GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC 
     4321 GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG 
     4381 ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA 
     4441 CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG 
     4501 CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA 
     4561 CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA 
     4621 GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC 
     4681 CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG 
     4741 ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG 
     4801 CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG 
     4861 CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG 
     4921 GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC 
     4981 GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC 
     5041 GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC 
     5101 CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC 
     5161 ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC 
     5221 AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC 
     5281 CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG 
     5341 CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA 
     5401 GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC 
     5461 TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT 
     5521 TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA 
     5581 ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA 
     5641 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 
     5701 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG 
     5761 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 
     5821 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 
     5881 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 
     5941 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 
     6001 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 
     6061 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 
     6121 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 
     6181 T
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