LOCUS       RR202260                8529 bp    DNA     circular  SYN 
DEFINITION  Ptpro (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, receptor type, O (Ptpro), (10 ug)
ACCESSION   NM_017336
VERSION     NM_017336.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Ptpro"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCACCTG CCTAGGGAAA GGCGCGGGGG CCGCCGCCTG CTACCTCTGC 
     1081 TCGGGCTCTT TGTGCTGCTC AAGATTGTTT CAACGTTCCA CGTGACTGTC CGAGACGACA 
     1141 ACAACATCGT TGTGTCTTTA GAAGCTTCTG ATGTAGTCAG CCCAACCTCC GTGTATGTTG 
     1201 TGAGGGTAGC TGGCGAATCC AAAAACTATT TCTTCGAATT TGAGGAATCC AATGGCACAC 
     1261 TGCCTCCTCC TGTGGTCTTT AAGGCCACGT ATCATGGTCT TTATTACATC ATCAGTCTGG 
     1321 TGGTAGTCAG TGGGAATGTG GTCACCAAAC CATCCCGATC AATCACCGTG CTGACAAAAC 
     1381 CCTTGCCCGT AACCAGTGTG TCTATATATG ACTATAAGCC TTCTCCTGAG ACAGGAGTAC 
     1441 TGTTTGAAAT TCACTATCCG GAAAAATACA ACGTGTTCAG CAGAGTGAAC ATCAGCTACT 
     1501 GGGAGGGGAA GGATTTCAGG ACGATGCTGT ACAAAGACTT CCTGAAGGGG AAAACCGTGT 
     1561 TTAATCACTG GCTCCCGGGG ATCTGCTACA GTAACATCAC TTTCCAGCTG GTTTCTGAGG 
     1621 CGACCTTTAA CAAAAGCACC CTGGTGGAGT GCAGTGGTGT GAGTCACGAA CCCAAACAAC 
     1681 ACAGAACAGC GCCGTATCCC CCTCGAAACA TCTCTGTTCG CTTTGTCAAC TTGAACAAGA 
     1741 ACAACTGGGA AGAACCAAGC GGGAGCTTCC CCGAGGACTC GTTCATCAAA CCGCAAGACG 
     1801 CGATAGGAAG AGACAGACCC TTCCATTTCC CCGAGGAAAC CCCTGAGACG CCCGCCAGCA 
     1861 ATGCGTCTTC CGGCTGGCCT GACCCGAACG GCACTGAGTA TGAGAGCACA TCTCAGCCCT 
     1921 ACTGGTGGGA CAGTGCATCC GCGGCCCCTG AAAACGAGGA GGACTTCGTC AGCGTGCTGC 
     1981 CAGCGGACTA TGACACTGAG GCCACGCTCG GCAGGACAGA GAAACCCACA GCTGACCCTT 
     2041 TCTCTGCCTT CCCCGTGCAG ATGACTCTGA GCTGGTTACC ACCAAAACCG CCTACAGCCT 
     2101 TCGATGGCTT CAATATCCTC ATAGAGAGGG AAGGGAACTT TACCGACTAT TTGACAGTGG 
     2161 ATGAAGAAGC CCACGAGTTT GTTGCCGAAC TGAAGGAGCC TGGAAAGTAC AGACTCTCAG 
     2221 TGACAACCTT CAGCTCCTCG GGAGCCTGTG AGACTCGGAA AAGCCAGTCA GCAAAATCGC 
     2281 TCAGCTTCTA CATCAGCCCC ACAGGAGAGT GGATTGAAGA ACTGACTGAG AAACCGCAGC 
     2341 ACGTGAGTGT CCACGTCTTA AGCTCAACCA CTGCCTTGTT GTCGTGGACA TCTTCCCAGG 
     2401 AGAACTACAA CAGCACCATT GTGTCTGTGG TGTCCCTGAC ATGCCAGAAA CAGAAAGAGA 
     2461 GCCAGCGGCT GGAGAAGCAG TATTGTACCC AGGTGAACTC AAGCAAACCT GTAATTGAGA 
     2521 ACCTGGTTCC CGGTGCCCAG TACCAGGTTG TGATGTACCT AAGAAAAGGC CCTTTGATTG 
     2581 GGCCACCTTC TGATCCTGTG ACATTTGCCA TTGTTCCCAC GGGGATAAAG GATTTAATGC 
     2641 TCTATCCATT GGGTCCCACG GCAGTAGTGC TGAGCTGGAC CCGACCTTAC TTGGGAGTGT 
     2701 TCAGAAAATA CGTGGTTGAA ATGTTTTATT TCAACCCCAC CACCATGACC TCCGAGTGGA 
     2761 CGACCTACTA CGAAATAGCA GCGACCGTCT CCTTAACATC GTCCGTGAGA ATAGCAAATC 
     2821 TATTGCCGGC GTGGTACTAC AACTTTCGCG TAACCATGGT GACGTGGGGA GATCCAGAAC 
     2881 TGAGCTGCTG TGACAGTTCC ACCATCAGCT TCATAACGGC CCCCGTCGCT CCAGAAATCA 
     2941 CGTCTGTGGA GTACTTCAAC AGCCTGCTGT ACATCAGCTG GACCTACGGG GACGCCACCA 
     3001 CTGACCTGTC CCATTCGAGA ATGCTACACT GGATGGTCGT GGCAGAAGGG AGGAGGAAAA 
     3061 TTAAAAAGAG TGTGACACGC AATGTCATGA CGGCCATCCT TAGCCTGCCT CCAGGAGATA 
     3121 TCTACAACCT GTCTGTCACA GCCTGCACTG AGAGGGGGAG TAACACCTCC ATGCTCCGCC 
     3181 TTGTCAAGCT AGAACCAGCT CCTCCAAAAT CACTCTTCGC AGTGAACAAA ACGCAGACAT 
     3241 CGGTCACCCT GCTTTGGGTT GAGGAGGGCG TAGCTGATTT CTTTGAAGTC CTCTGTCAGA 
     3301 AGCTCGGGTC TGGCCACGAT GGCAAACTCC AGGAACCGGT AGCTGTTTCC TCCCACGTGG 
     3361 TGACCATCTC CAGCCTCCTC CCAGCCACTG CCTACAACTG CAGTGTCACC AGCTTCAGCC 
     3421 ACGACAGTCC CAGTGTCCCT ACGTTCATAG CTGTCTCCAC AATGGTTACA GAGGTGAACC 
     3481 CTAATGTGGT GGTGATCTCG GTGCTGGCCA TCCTCAGCAT ACTTTTAATT GGACTGCTGT 
     3541 TAGTGACCCT TGTCATTCTG AGAAAGAAGC ACCTGCAGAT GGCCAGGGAG TGTGGGGCTG 
     3601 GCACGTTTGT CAATTTTGCA TCCTTGGAGA GGGAAGGGAA ACTCCCCTAC AGTTGGCGTA 
     3661 GGAGTGTCTT TACTTTCTTA ACCCTGCTGC CCTCATGTCT GTGGACTGAC TATCTGTTGG 
     3721 CATTTTATAT TAACCCTTGG AGTAAAAATG GCTTAAAGAA GAGGAAACTG ACGAACCCTG 
     3781 TTCAGCTGGA CGATTTTGAT TCTTACATCA AGGACATGGC CAAGGACTCT GACTATAAAT 
     3841 TCTCTCTTCA GTTTGAGGAG TTGAAGTTGA TTGGACTGGA TATTCCACAC TTTGCTGCAG 
     3901 ATCTTCCGCT GAACCGATGT AAAAACCGCT ATACAAACAT CCTGCCGTAC GACTTCAGCA 
     3961 GAGTGAGGCT GGTCTCCATG AATGAAGAAG AAGGAGCAGA CTACATTAAT GCCAATTATA 
     4021 TTCCTGGCTA CAACTCACCT CAGGAGTACA TTGCCACCCA GGGACCACTG CCGGAAACCA 
     4081 GAAATGATTT CTGGAAGATG GTCCTGCAGC AGAAGTCTCG CATCATTGTC ATGCTCACTC 
     4141 AGTGCAATGA GAAAAGAAGG GTAAAATGCG ACCACTACTG GCCATTCACG GAAGAACCCA 
     4201 TTGCTTATGG AGACATCACC GTGGAGATGG TCTCTGAGGA AGAACAGGAG GACTGGGCCA 
     4261 GCAGACACTT CCGGATCAAT TATGCTGACG AGGCCCAGGA TGTGATGCAC TTTAACTACA 
     4321 CGGCCTGGCC AGACCATGGC GTGCCTCCAG CAAATGCTGC CGAGAGCATC CTGCAGTTCG 
     4381 TGTACACTGT CCGGCAGCAA GCCACCAAGA GCAAAGGACC CATGATCATC CACTGCAGCG 
     4441 CGGGCGTGGG ACGGACAGGC ACCTTCATAG CCCTTGACAG GCTCTTGCAA CACATCCGAG 
     4501 ATCACGAATT TGTGGACATC TTGGGGCTGG TGTCAGAAAT GCGCTCATAC CGAATGTCGA 
     4561 TGGTACAGAC AGAGGAGCAG TACATCTTCA TCCATCAGTG CGTGCAGCTG ATGTGGCTGA 
     4621 AGAAGAAGCA ACAGTTCTGC ATCAGTGACG TCATCTACGA GAACGTCAGC AAGTCCACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//