LOCUS       RR202215                6317 bp    DNA     circular  SYN 
DEFINITION  Coro1a (Myc-DDK-tagged ORF) - Rat coronin, actin binding protein 1A (Coro1a), (10 ug)
ACCESSION   NM_130411
VERSION     NM_130411.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2474..2565 
                     /label="Myc-DDK"  
     misc_feature    2595..3180 
                     /label="PolyA signal"  
     misc_feature    2652..2671 
                     /label="Restriction Enzyme"  
     misc_feature    3456..4128 
                     /label="ColE1"  
     misc_feature    4558..5352 
                     /label="Kan/Neo"  
     misc_feature    5387..5689 
                     /label="SV40 ori"  
     misc_feature    5775..6252 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2456..2462 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2456 
                     /label="Coro1a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGGCAG GTGGTTCGCT CCAGCAAATT CCGCCACGTG TTTGGACAGC 
     1081 CAGCCAAAGC TGACCAGTGC TATGAGGATG TGCGCGTCTC ACAAACCACT TGGGACAGTG 
     1141 GCTTCTGCGC TGTCAACCCC AAGTTCATGG CTCTGATCTG TGAGGCCAGT GGGGGAGGGG 
     1201 CCTTCCTGGT GCTACCCCTA GGCAAGACTG GACGAGTAGA CAAGAATGTG CCCCTGGTGT 
     1261 GCGGCCACAC TGCCCCTGTG CTAGACATCG CCTGGTGTCC ACACAATGAC AACGTCATTG 
     1321 CCAGTGGCTC TGAGGACTGC ACAGTTATGG TGTGGGAGAT CCCGGATGGG GGCCTGGTGC 
     1381 TGCCCTTGCG GGAGCCTGTT GTCACCTTGG AGGGCCACAC CAAGCGGGTT GGCATCGTGG 
     1441 CCTGGCACCC CACAGCCCAG AATGTACTGC TCAGCGCAGG TTGTGACAAT GTGATCCTGG 
     1501 TGTGGGATGT GGGCACTGGG GCAGCCGTGC TGACGCTGGG CCCGGATGTG CACCCGGACA 
     1561 CAATCTACAG TGTGGATTGG AGCCGGGATG GTGCTCTCAT CTGTACCTCC TGCCGTGACA 
     1621 AGCGTGTTCG CGTCATCGAG CCTCGCAAAG GCACTGTTGT GGCTGAGAAG GAGCGTCCTC 
     1681 ATGAGGGAAC TCGGCCAGTG CATGCTGTGT TTGTGTCAGA AGGGAAGATT CTAACCACGG 
     1741 GGTTCAGTCG CATGAGTGAG CGGCAGGTGG CGCTGTGGGA CACAAAGCAC CTGGAGGAGC 
     1801 CACTGTCCCT GCAGGAGCTG GACACAAGCA GTGGAGTCCT GCTACCTTTT TTTGACCCTG 
     1861 ATACCAACAT CGTCTACCTC TGTGGCAAGG GTGACAGCTC TATCCGGTAT TTTGAGATCA 
     1921 CGTCTGAGGC TCCGTTCCTG CATTATCTTT CCATGTTCAG TTCCAAGGAG TCTCAACGTG 
     1981 GTATGGGCTA CATGCCCAAA CGTGGCCTGG AAGTGAATAA GTGCGAGATT GCCAGATTCT 
     2041 ATAAGCTTCA TGAGCGGAAA TGTGAGCCTA TTGCCATGAC AGTGCCTAGA AAGTCGGACC 
     2101 TGTTCCAGGA GGACCTGTAT CCACCCACTG CCGGACCCGA CCCCGCCCTC ACAGCTGAGG 
     2161 AGTGGCTGAG CGGTCGTGAT GCTGGCCCCC TCCTCATTTC CCTCAAGGAT GGCTACGTGC 
     2221 CCCCAAAGAG CAGGGAGCTG AGAGTCAACA GGGGCCTGGA CAGTGCTCGC AGAAGAGCTA 
     2281 CACCGGAGCC CAGTAGCACT CTTAGCTCGG ACACCGTGTC ACGGCTGGAG GAGGATGTGA 
     2341 GGAATCTCAA CGCCATAGTG CAAAAGCTTC AAGAGCGCCT GGACAGGCTG GAGGAGACTG 
     2401 TTCAGGCCAA GTGCAAAAGC TTCAAGAGCG CCTGGACAGG CTGGAGGAGA CTGTTCAGGC 
     2461 CAAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     2521 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG 
     2581 CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG 
     2641 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA 
     2701 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA 
     2761 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA 
     2821 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC 
     2881 TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT 
     2941 TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT 
     3001 CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG 
     3061 CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC 
     3121 AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG 
     3181 TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG 
     3241 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 
     3301 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     3361 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     3421 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     3481 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     3541 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     3601 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     3661 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     3721 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     3781 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     3841 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     3901 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     3961 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     4021 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 
     4081 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC 
     4141 GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA 
     4201 AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC 
     4261 CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC 
     4321 TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA 
     4381 GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC 
     4441 ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG 
     4501 GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA 
     4561 GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG 
     4621 CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA 
     4681 TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT 
     4741 CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT 
     4801 GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG 
     4861 CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA 
     4921 TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG 
     4981 GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG 
     5041 CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC 
     5101 CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG 
     5161 ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA 
     5221 AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG 
     5281 TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT 
     5341 GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG 
     5401 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG 
     5461 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG 
     5521 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC 
     5581 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC 
     5641 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 
     5701 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT 
     5761 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     5821 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     5881 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     5941 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     6001 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     6061 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     6121 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     6181 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     6241 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     6301 AATTTAACGC GAATTTT
//