LOCUS       RR202204                6631 bp    DNA     circular  SYN 
DEFINITION  Dmrt2 (Myc-DDK-tagged ORF) - Rat doublesex and mab-3 related transcription factor 2 (Dmrt2), (10 ug)
ACCESSION   NM_001107597
VERSION     NM_001107597.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2788..2879 
                     /label="Myc-DDK"  
     misc_feature    2909..3494 
                     /label="PolyA signal"  
     misc_feature    2966..2985 
                     /label="Restriction Enzyme"  
     misc_feature    3770..4442 
                     /label="ColE1"  
     misc_feature    4872..5666 
                     /label="Kan/Neo"  
     misc_feature    5701..6003 
                     /label="SV40 ori"  
     misc_feature    6089..6566 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2770..2776 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2770 
                     /label="Dmrt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGGGCAG GCAGTCCCCG GAGTTGGGGA CTGGGAGATC GACGTGGAGA 
     1081 GCCTAGAGCT GGAGGAGGAT GGCTGTGGGA CACCGTTGCG GGCCACGCCA CAGGAACCCA 
     1141 GTCCGTCGAC GGCCGATGGA GAGGAAGATG AGGACGAAGA AGAGGAAGAT GAAGATGCAG 
     1201 AGGATGAGGG TGAAGGCGAG GACTCCAGCG CGTCCCCGGA GGTACCAGGT CGGCCAGAGC 
     1261 AGCCCGGGGG ACTGGCACCC AGGCCACCGC CCGCTGCTCA GGCGCTGCCA GCCGCCGCCG 
     1321 TAGTCCCCGA GCGCGGTGTC ACCGCCGGTG GCGGCGCAGA GCCCCGTAAA CTGAGCCGCA 
     1381 CGCCCAAGTG CGCACGCTGC CGTAACCACG GCGTGGTGTC CTGTCTCAAG GGCCACAAGC 
     1441 GATTCTGTCG CTGGCGGGAC TGCCAGTGCG CCAACTGCCT GCTTGTGGTA GAGCGCCAGC 
     1501 GAGTCATGGC CGCCCAGGTG GCGCTCCGGA GGCAGCAGGC CACAGAGGAC AAGAAGGGGC 
     1561 TTTCTGGGAA ACAGAATAAT TTCGATCGGA AAGCAGTGTA CCAGAGGCAA GTCAGGGCGC 
     1621 CAAGCTTGCT GGCCAAAAGC ATTTTAGAAG GGTACCGCCC AATGACGGCG GCCGAGACTT 
     1681 ACCTAGGAGG GACCCTTCCC CTACCCCCGC CTGTCAGTGA CAGGATGAGG AAGAGAAGAG 
     1741 CATTTGCTGA CAAGGAGTTG GAGAACATTA TGCTGGAGAG AGAATATAAA GAGAGGGAGA 
     1801 TGTTAGAAAC ATCTCAAGCC GCTGCCTTGT TTCTGCCCAA TCGCATGGTG CCCGGGCCTG 
     1861 AGTACAGTTC CTACAAAGGT GCCTTCAGCC CCAGCGCAGG CGAGCTGCCG AGCAAGGACT 
     1921 TCTGTAACTT TTTACCTACC TGTCTCGATC TCACCATGCA GTATTCAGGG TCTGGGAATA 
     1981 TGGAACTGAT TTCTTCCAAT GTCAGCGTGG CCACAACTTA CAGACAATAT CCCCTGTCCT 
     2041 CGCGATTTTT AGTCTGGCCC AAGTGCGGTC CCATTAGCGA CACCCTCCTC TACCAGCAAT 
     2101 ACCTGTTAAA TGCTACCACT TCTGTCCAAG CCCTGAAGCC TGGGGCCGGC TGGGACTTGA 
     2161 AAGGAACCCG AGTTCAGGAT GGGCTTAGTG CAGAGCATGA CGGGATGCCA CCCAAACCGG 
     2221 AAGGCTCTCT AGTGCTGCCT CAACTATCAG AGGTCCCGAC CTCAAGAACT GACCTTCAGG 
     2281 CTCACCAGGC TGTCCCAGAG AGGTCTGCCT TCTCCCCACC GAGCCGGAAT TTCTCCCCCA 
     2341 TTGTTGATAT GGACTGTCTG GCAGCTCAAG GGCACGTCTT AACCAAGATC AGCAAAGAAA 
     2401 ACACTAGGTA TCCTCTGCCA CTTAAACGTA ATCCATTCCA CTCAGTATTC CAGCCGACGC 
     2461 TCAACGACAA ATCAGGCCCT GAATTGAAAG CACCGTTTGT CAAAGAAGCC TTTGAAGAGA 
     2521 CCCCGAAGAA ACACAGGGAG TGTTTGGTCA AGGAGAACCC GAAGTACACA TTTACAATAG 
     2581 ACAGATGCGC AAAAGACCTC TTCGTAGCCA AACAAGTTGG AACGAAACTC TCGGTGAACG 
     2641 AGCCTCTGTC GTTTTCCGTG GAATCTATTC TTAAGAGGCC CTCATCTGCT GTCACTCCCG 
     2701 TCTCTCAGCG AGCCTCTGTC GTTTTCCGTG GAATCTATTC TTAAGAGGCC CTCATCTGCT 
     2761 GTCACTCCCG TCTCTCAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG 
     2821 GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC 
     2881 GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC 
     2941 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA 
     3001 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG 
     3061 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG 
     3121 GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT 
     3181 TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG 
     3241 GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT 
     3301 CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA 
     3361 GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA 
     3421 GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG 
     3481 CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG 
     3541 TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     3601 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     3661 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     3721 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     3781 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     3841 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     3901 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     3961 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     4021 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     4081 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     4141 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     4201 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     4261 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     4321 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     4381 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA 
     4441 TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG 
     4501 GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG 
     4561 TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC 
     4621 CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT 
     4681 CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC 
     4741 CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT 
     4801 AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA 
     4861 TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG 
     4921 CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC 
     4981 AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC 
     5041 CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA 
     5101 GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT 
     5161 GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC 
     5221 AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA 
     5281 TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC 
     5341 TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG 
     5401 CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT 
     5461 CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG 
     5521 GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC 
     5581 AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC 
     5641 CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC 
     5701 TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA 
     5761 GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG 
     5821 GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA 
     5881 CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     5941 GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG 
     6001 CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC 
     6061 CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 
     6121 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 
     6181 TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 
     6241 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 
     6301 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 
     6361 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 
     6421 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 
     6481 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 
     6541 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 
     6601 GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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