LOCUS       RR202187                8698 bp    DNA     circular  SYN 
DEFINITION  Cyfip1 (Myc-DDK-tagged ORF) - Rat cytoplasmic FMR1 interacting protein 1 (Cyfip1), (10 ug)
ACCESSION   NM_001107517
VERSION     NM_001107517.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4853..4944 
                     /label="Myc-DDK"  
     misc_feature    4974..5559 
                     /label="PolyA signal"  
     misc_feature    5031..5050 
                     /label="Restriction Enzyme"  
     misc_feature    5835..6507 
                     /label="ColE1"  
     misc_feature    6937..7731 
                     /label="Kan/Neo"  
     misc_feature    7766..8068 
                     /label="SV40 ori"  
     misc_feature    8154..8631 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4828..4835 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4828 
                     /label="Cyfip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCCAA GTGACACTAG AGGACGCACT GTCCAATGTG GACCTCCTGG 
     1081 AAGAGCTGCC TCTTCCTGAC CAGCAGCCCT GCATTGAGCC CCCACCTTCC TCGCTGCTCT 
     1141 ATCAGCCAAA TTTCAACACC AACTTTGAAG ACCGAAATGC ATTTGTCACT GGCATTGCAA 
     1201 GATACATTGA ACAAGCAACT GTCCACTCTA GCATGAACGA GATGCTAGAG GAAGGCCAAG 
     1261 AGTATGCTGT CATGCTGTAT ACCTGGAGGA GCTGCTCCCG TGCCATCCCT CAGGTGAAGT 
     1321 GCAATGAGCA GCCTAATAGG GTTGAAATTT ATGAGAAAAC TGTGGAAGTC CTTGAACCCG 
     1381 AGGTCACAAA ACTGATGAAT TTTATGTACT TCCAGAGAAA TGCCATCGAG CGTTTCTGTG 
     1441 GGGAGGTGAG ACGCCTGTGT CATGCTGAGA GGAGAAAGGA CTTCGTGTCT GAAGCCTACT 
     1501 TGATCACCTT GGGCAAGTTT ATCAACATGT TTGCTGTGTT GGATGAGCTG AAGAACATGA 
     1561 AGTGCAGTGT GAAGAATGAC CACTCAGCGT ATAAGAGGGC TGCTCAGTTT TTACGTAAAA 
     1621 TGGCAGATCC ACAATCCATC CAGGAGTCAC AGAATTTGTC CATGTTCCTG GCCAACCACA 
     1681 ACAAGATTAC ACAATCTCTA CAGCAACAGC TTGAGGTCAT TTCTGGCTAT GAAGAACTCC 
     1741 TGGCAGACAT AGTGAATCTG TGTGTGGACT ACTATGAGAA CAGAATGTAC TTGACGCCCA 
     1801 GCGAAAAACA CATGCTTCTC AAAGTCATGG GATTTGGTCT GTACCTGATG GATGGGAGTG 
     1861 TGAGTAACAT CTACAAACTA GATGCCAAGA AAAGAATAAA TTTATCCAAA ATAGACAAGT 
     1921 ATTTCAAGCA GCTGCAGGTG GTTCCACTCT TTGGAGATAT GCAGATAGAA CTGGCAAGAT 
     1981 ACATCAAGAC CAGCGCACAC TATGAGGAGA ATAAGTCCCG GTGGACCTGT ACGTCCTCGA 
     2041 GCAGCAGCCC ACAGTACAAC ATCTGTGAGC AGATGATCCA GATCCGTGAG GACCACATGC 
     2101 GTTTCATTTC TGAGCTGGCA CGCTACAGCA ATAGCGAGGT AGTCACAGGG TCTGGCCGGC 
     2161 AGGAGGCCCA GAAGACAGAT GCTGAGTACC GGAAGCTCTT TGACCTGGCG CTGCAGGGCC 
     2221 TGCAGCTTCT GTCGCAGTGG AGCGCGCATG TGATGGAAGT GTATTCCTGG AAACTTGTAC 
     2281 ATCCAACAGA CAAGTACTCC AACAAGGACT GCCCAGACAA TGCTGAGGAG TATGAACGTG 
     2341 CCACGCGTTA CAACTACACC ACTGAGGAGA AGTTTGCTCT GGTGGAGGTG ATTGCCATGA 
     2401 TCAAAGGCCT GCAGGTGCTG ATGGGCAGGA TGGAGAGTGT GTTCAATCAC GCCATCAGAC 
     2461 ACACAGTTTA TGCTGCACTT CAGGACTTCT CCCAGGTGAC CCTCAGGGAA CCACTGCGAC 
     2521 AGGCCATCAA GAAGAAAAAG AATGTCATCC AGAGTGTCCT ACAGGCCATC AGGAAGACTG 
     2581 TGTGTGACTG GGAGACTGGG CATGAACCCT TTAACGACCC AGCTCTGCGG GGAGAGAAGG 
     2641 ACCCTAAGAG CGGCTTTGAC ATCAAGGTGC CACGCCGTGC TGTAGGACCC TCCAGCACAC 
     2701 AGCTTTACAT GGTGAGAACT ATGCTAGAGT CCCTCATTGC AGACAAAAGT GGTTCCAAGA 
     2761 AAACCTTGAG AAGTAGCCTT GAGGGGCCCA CCATATTGGA CATAGAAAAA TTTCATCGAG 
     2821 AATCATTCTT CTACACTCAC TTGATAAATT TCAGTGAAAC ACTGCAGCAG TGCTGTGACC 
     2881 TTTCCCAGCT GTGGTTCCGA GAGTTCTTCC TGGAGCTGAC CATGGGCAGG AGGATCCAGT 
     2941 TCCCCATTGA GATGTCTATG CCCTGGATTC TGACTGACCA CATCCTAGAG ACCAAGGAGG 
     3001 CATCAATGAT GGAGTACGTC CTCTACTCCT TGGACCTGTA CAATGACAGT GCTCACTATG 
     3061 CGCTCACCAA GTTCAACAAG CAGTTTCTCT ATGATGAAAT TGAAGCAGAG GTGAATCTGT 
     3121 GTTTTGACCA ATTTGTTTAT AAACTGGCAG ACCAGATATT TGCATATTAC AAGGTTATGG 
     3181 CAGGAAGTTT GCTTCTTGAC AAACGGTTAC GATCAGAATG CAAAAATCAG GGAGCCACGA 
     3241 TTCACCTCCC ACCGTCTAAC CGCTATGAGA CGTTGTTGAA GCAAAGGCAC GTGCAGCTGC 
     3301 TTGGGAGATC CATCGATCTC AATCGTCTGA TTACACAGCG TGTTTCAGCA GCCATGTACA 
     3361 AATCTCTAGA ACTGGCAATT GGACGATTTG AAAGTGAAGA TTTAACATCA GTAGTCGAGC 
     3421 TAGATGGGCT CTTAGAAATC AACCGTATGA CGCACAAGCT GCTGAGTAGG TACCTCACAC 
     3481 TAGACAGCTT TGATGCCATG TTCCGGGAAG CCAACCACAA CGTGTCTGCA CCTTATGGAA 
     3541 GAATCACCCT CCATGTCTTC TGGGAGCTAA ACTATGACTT CTTGCCCAAC TACTGCTATA 
     3601 ATGGCTCCAC CAACCGGTTT GTTCGGACAG TACTACCATT TTCTCAGGAA TTTCAACGAG 
     3661 ACAAACAACC TAATGCACAG CCCCAGTATT TGCACGGCTC CAAGGCTTTG AACCTAGCCT 
     3721 ACTCAAGCAT TTATGGCAGC TACCGGAACT TTGTGGGGCC TCCTCACTTC CAGGTCATCT 
     3781 GCCGGCTGCT TGGCTACCAG GGCATTGCAG TCGTCATGGA AGAACTGCTG AAGGTTGTCA 
     3841 AGAGCCTGCT TCAAGGCACA ATCCTGCAGT ATGTGAAAAC TCTGATGGAA GTGATGCCTA 
     3901 AGATCTGCCG CCTTCCCCGA CATGAGTACG GCTCTCCTGG CATCCTAGAG TTCTTCCACC 
     3961 ACCAGCTGAA GGACATTGTG GAGTATGCAG AGCTGAAAAC AGTATGCTTC CAGAACCTGC 
     4021 GGGAGGTGGG AAATGCTGTT CTCTTCTGCC TGCTCATCGA GCAAAGCCTG TCTTTAGAAG 
     4081 AAGTCTGTGA CCTGTTGCAT GCAGCTCCTT TTCAGAATAT CCTGCCTCGA GTCCACGTAA 
     4141 AAGAGGGGGA GAGAGTTGAT GCCAAAATGA AAAGACTAGA ATCCAAGTAT GCTCCACTGC 
     4201 ATCTTGTTCC GCTGATTGAA AGGCTGGGGA CCCCACAGCA AATTGCAATC GCAAGAGAGG 
     4261 GGGACTTGCT GACCAAGGAG CGGCTCTGTT GTGGTCTGTC CATGTTTGAA GTCATCCTCA 
     4321 CACGGATCCG GACCTTTCTG GATGATCCCA TCTGGCGTGG GCCCCTGCCC AGCAATGGGG 
     4381 TCATGCATGT GGATGAGTGT GTGGAGTTTC ACAGACTATG GAGTGCCATG CAGTTTGTTT 
     4441 ACTGCATTCC TGTAGGGACA CACGAGTTTA CAGTGGAGCA GTGTTTTGGC GATGGGCTCC 
     4501 ACTGGGCTGG CTGCATGATC ATTGTACTTC TGGGACAGCA GCGGCGCTTT GCTGTGTTGG 
     4561 ATTTCTGCTA TCATCTTCTC AAAGTTCAGA AACACGATGG CAAGGATGAG ATCATCAAAA 
     4621 ATGTGCCATT GAAGAAGATG GTGGAGAGGA TTCGCAAGTT CCAGATTCTC AATGATGAGA 
     4681 TCATCACTAT CCTGGACAAG TACTTGAAAT CTGGAGATGG GGAGAGCACA CCTGTGGAAC 
     4741 ATGTACGCTG CTTCCAGCCA CCCATCCACC AGTCCCTAGC CAGCAGCACA TGTACGCTGC 
     4801 TTCCAGCCAC CCATCCACCA GTCCCTAGCC AGCAGCAGCG GACCGACGCG TACGCGGCCG 
     4861 CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG 
     4921 GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC 
     4981 CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC 
     5041 AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC 
     5101 CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC 
     5161 AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG 
     5221 GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT 
     5281 GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG 
     5341 GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC 
     5401 TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT 
     5461 GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA 
     5521 TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC 
     5581 CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT 
     5641 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     5701 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     5761 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     5821 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     5881 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     5941 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     6001 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     6061 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     6121 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     6181 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     6241 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 
     6301 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     6361 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     6421 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     6481 AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA 
     6541 GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT 
     6601 ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC 
     6661 CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA 
     6721 TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT 
     6781 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA 
     6841 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG 
     6901 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC 
     6961 GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC 
     7021 GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC 
     7081 TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG 
     7141 GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC 
     7201 GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG 
     7261 CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC 
     7321 GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG 
     7381 CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG 
     7441 ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC 
     7501 GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC 
     7561 TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG 
     7621 CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA 
     7681 GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT 
     7741 CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA 
     7801 AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT 
     7861 AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG 
     7921 GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT 
     7981 GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA 
     8041 GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT 
     8101 GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG 
     8161 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 
     8221 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG 
     8281 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 
     8341 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 
     8401 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 
     8461 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 
     8521 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 
     8581 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 
     8641 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//