LOCUS       RR202166                6113 bp    DNA     circular  SYN 
DEFINITION  Ptrf (Myc-DDK-tagged ORF) - Rat polymerase I and transcript release factor (Ptrf), (10 ug)
ACCESSION   NM_001105841
VERSION     NM_001105841.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2270..2361 
                     /label="Myc-DDK"  
     misc_feature    2391..2976 
                     /label="PolyA signal"  
     misc_feature    2448..2467 
                     /label="Restriction Enzyme"  
     misc_feature    3252..3924 
                     /label="ColE1"  
     misc_feature    4354..5148 
                     /label="Kan/Neo"  
     misc_feature    5183..5485 
                     /label="SV40 ori"  
     misc_feature    5571..6048 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2252..2258 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2252 
                     /label="Ptrf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATGTC ACGCTCCATA TCGTCGAGCG GCCGTATTCC GGATATCCCG 
     1081 ATGCTTCCTC CGAGGGCCCG GAGCCCACCC CAGGGGAGGC ACGGGCCACC GAGGAGCCGT 
     1141 CGGGGACCGG TTCCGACGAG CTGATCAAGT CGGACCAGGT AAACGGTGTA CTGGTGCTGA 
     1201 GCCTTCTGGA TAAAATCATC GGCGCCGTTG ACCAGATCCA GCTGACCCAA GCCCAGCTGG 
     1261 AAGAGCGACA GGCAGAGATG GAGGGCGCTG TGCAGAGCAT CCAGGGAGAG CTGAGCAAGC 
     1321 TGGGCAAGGC GCACGCCACC ACCAGCAACA CCGTGAGCAA GTTGCTGGAG AAGGTGCGCA 
     1381 AGGTCAGCGT CAACGTGAAG ACGGTGCGAG GCAGCCTGGA GCGCCAGGCT GGCCAGATAA 
     1441 AGAAACTGGA GGTCAACGAG GCGGAGCTGC TGCGGCGCCG CAACTTCAAA GTCATGATCT 
     1501 ACCAGGATGA AGTCAAGCTG CCGGCCAAAC TGAGCGTCAG CAAGTCTCTG AAAGAGTCGG 
     1561 AGGCGCTGCC AGAGAAGGAG GGCGATGAGC TGGGCGAGGG CGAGCGGCCG GAGGAGGACG 
     1621 CTGCGGCGAT CGAGCTGTCG TCTGACGAGG CGGTGGAGGT GGAGGAGGTG ATCGAGGAGT 
     1681 CCCGCGCCGA GCGCATCAAG CGCAGCGGCC TGCGGCGCGT GGACGACTTC AAGAAGGCCT 
     1741 TCTCCAAGGA GAAGATGGAG AAGACCAAGG TGCGCACGCG CGAGAACCTG GAGAAGACGC 
     1801 GCCTGAAGAC TAAGGAGAAC CTGGAGAAGA CGCGGCACAC GCTGGAGAAG CGCATGAACA 
     1861 AGCTGGGCAC GCGCCTGGTG CCCGTGGAGC GACGCGAGAA GCTGAAGACG TCGCGCGACA 
     1921 AGCTGCGCAA GTCCTTCACG CCCGACCACG TGGTGTACGC GCGCTCCAAG ACCGCGGTCT 
     1981 ACAAGGTGCC GCCCTTTACC TTCCATGTCA AGAAGATCCG CGAGGGCGAG GTGGAGGTGC 
     2041 TGAAGGCCAC CGAGATGGTG GAGGTGGGTC CTGATGACGA CGAGGTTGGC GCGGAGCGCG 
     2101 GCGAGGCCAC TGACCTGCTG CGCGGGAGCA GCCCCGACGT GCACACGCTG CTGGAGATCA 
     2161 CCGAGGAATC GGACGCCGTC CTGGTGGACA AGAGCGACAG CGACTGCTGG AGATCACCGA 
     2221 GGAATCGGAC GCCGTCCTGG TGGACAAGAG CGACAGCGAC ACGCGTACGC GGCCGCTCGA 
     2281 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     2341 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT 
     2401 GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC 
     2461 CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT 
     2521 ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT 
     2581 GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA 
     2641 CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG 
     2701 GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT 
     2761 TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC 
     2821 CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT 
     2881 TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC 
     2941 AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC 
     3001 AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG 
     3061 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 
     3121 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 
     3181 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 
     3241 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 
     3301 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 
     3361 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 
     3421 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 
     3481 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 
     3541 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 
     3601 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 
     3661 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 
     3721 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 
     3781 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 
     3841 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 
     3901 ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT 
     3961 GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG 
     4021 CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT 
     4081 TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG 
     4141 CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG 
     4201 AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG 
     4261 ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG 
     4321 TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA 
     4381 GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA 
     4441 AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT 
     4501 CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT 
     4561 TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT 
     4621 CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT 
     4681 CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC 
     4741 GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA 
     4801 TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT 
     4861 GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA 
     4921 CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA 
     4981 GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG 
     5041 ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA 
     5101 ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC 
     5161 GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG 
     5221 CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA 
     5281 AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG 
     5341 ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA 
     5401 CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC 
     5461 ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC 
     5521 ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA 
     5581 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     5641 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     5701 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     5761 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     5821 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     5881 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     5941 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     6001 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     6061 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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