LOCUS       RR202153                8479 bp    DNA     circular  SYN 
DEFINITION  Plcb2 (Myc-DDK-tagged ORF) - Rat phospholipase C, beta 2 (Plcb2), (10 ug)
ACCESSION   NM_053478
VERSION     NM_053478.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4636..4727 
                     /label="Myc-DDK"  
     misc_feature    4757..5342 
                     /label="PolyA signal"  
     misc_feature    4814..4833 
                     /label="Restriction Enzyme"  
     misc_feature    5618..6290 
                     /label="ColE1"  
     misc_feature    6720..7514 
                     /label="Kan/Neo"  
     misc_feature    7549..7851 
                     /label="SV40 ori"  
     misc_feature    7937..8414 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4618..4624 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4618 
                     /label="Plcb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTTGCTC AACCCTGTCC TGTTGCCCCC TAAGGTGAAG GCATATCTGA 
     1081 GCCAAGGGGA GCGCTTCATC AAGTGGGACG ATGAAACCTC AATAGCCTCC CCTGTTATCC 
     1141 TTCGGGTGGA TCCCAAGGGC TATTACTTAT ACTGGACACA TCAAAGTAAG GAGATGGAAT 
     1201 TTCTGGATGT CACGAGCATC CGAGACACTC GCTTTGGGAA GTTTGCCAAG ATACCCAAGA 
     1261 GCCAGAAACT CCGGGAGGTA TTCAACATGG ACTTTCCAGA CAACCACTTC CTGCTGAAAA 
     1321 CATTCACAGT GGTATCCGGC CCGGACATGG TGGGCCTCAC CTTCCACAAT TTTGTCTCTT 
     1381 ACAAGGAGAA CGTAGGCAAG GATTGGGCTG AGGATGTACT GGCTCTGGCC AAACACCCAA 
     1441 TGACAGCCAA TGCTTCTCGA AGCACATTCC TAGACAAGAT CCTGGTGAAG CTTAAAATGC 
     1501 AGCTGAGCCC TGAAGGGAAG ATCCCTGTGA AGAATTTTTT CCAGATGTTT CCTGCCGATC 
     1561 GCAAACGTGT AGAAGCTGCC CTCAGTGCCT GTCACCTTGC AAAAGGCAAG AATGATGCTA 
     1621 TCAATCCCGA GGACTTCCCA GAATCTGTGT ACAAGAGCTT CCTCATGAGC CTCTGTCCTC 
     1681 GGCCAGAAAT TGATGAGATC TTCACTTCTT ATCATGCTAA AGCCAAACCC TACATGACCA 
     1741 AGGAGCACCT GACCAAATTC ATCAATCAGA AGCAACGAGA CCCTCGACTC AATTCCTTGC 
     1801 TGTTCCCACC AGCCCGGCCT GAGCAGGTGC AGGCGCTCAT TGACAAGTAC GAGCCCAGCG 
     1861 GCATCAATGT GCAGAGGGGC CAACTGTCAC CAGAGGGCAT GGTCTGGTTT CTCTGTGGAC 
     1921 CAGAGAACAG TGTCCTGGCC CATGATACTC TGCGGATCCA CCAAGACATG ACACAGCCTC 
     1981 TGAATCACTA CTTCATCAAC TCCTCACACA ACACTTACCT GACAGCTGGC CAGTTTTCAG 
     2041 GCCCTTCCTC GGCTGAGATG TACCGCCAGG TGCTGCTATC TGGCTGCCGT TGTGTAGAAT 
     2101 TAGATTGTTG GAAAGGAAAG CCCCCCGACG AAGAACCCAT CATCACCCAC GGCTTCACTA 
     2161 TGACCACAGA TATCTTGTTC AAGGAAGCAG TCGAAGCCAT TGCAGAAAGT GCCTTTAAGA 
     2221 CCTCCCCATA TCCTGTCATC CTGTCATTTG AAAACCACGT GGACTCACCC CGCCAACAGG 
     2281 CTAAGATGGC CGAGTACTGC CGGACCATGT TTGGAGAGAC CTTGCTCACA GAGCCCCTGG 
     2341 AAAACTTTCC TCTGAAACCT GGCATGCCTC TGCCTAGCCC TGAGGACCTC CGGGGCAAGA 
     2401 TCCTCATTAA GAATAAGAAG AACCAGTTTT CTGGGCCAGC ATCCCCCAAC AAGAAGCCTG 
     2461 ATGGGGTGTC TGAGGGCGGC TTCCCATCTA GTGTCCCTGT AGAAGAGGAC ACGGGGTGGA 
     2521 CTGCTGAGGA CCGGACTGAG GTGGAGGAGG GAGAGGAGGA GGAGGAGGTG GAAGAAGAGG 
     2581 AAGAGGAGGA GTCAGGAAAC CTGGATGAAG AAGAAATTAA GAAGATGCAG TCTGATGAGG 
     2641 GCACAGCAGG CTTGGAGGTG ACAGCTTATG AGGAAATGTC CAGCCTTGTC AATTACATCC 
     2701 AGCCCACCAA GTTTATCTCC TTCGAGTTCT CTGCACAGAA GAACAGGAGT TACCTTGTCT 
     2761 CTTCCTTCAC GGAGCTCAAG GCCTACGAGC TGCTCTCCAA GGCCTCCATG CAGTTTGTGG 
     2821 ACTATAATAA ACGCCAGATG AGCCGCGTGT ACCCCAAGGG CACGCGCATG GACTCTTCCA 
     2881 ACTACATGCC CCAGATGTTC TGGAATGCCG GATGTCAGAT GGTTGCCCTC AATTTCCAGA 
     2941 CAATGGATCT GCCCATGCAG CAGAACATGG CACTGTTTGA GTTCAACGGG CAGAGTGGCT 
     3001 ACCTCCTAAA GCACGAGTTC ATGCGCAGGC AGGACAAGCA GTTCAACCCC TTCTCGGTGG 
     3061 ACCGCATTGA TGTGGTGGTT GCCACTACCC TTTCCATTAC GGTCATCTCT GGGCAGTTCC 
     3121 TGTCAGAGCG CAGTGTGCGC ACCTATGTGG AAGTAGAACT GTTTGGCCTC CCAGGGGACC 
     3181 CCAAGAGGCG CTATCGTACC AAGCTGTCAC CTACTGCTAA CTCCATCAAT CCTGTCTGGA 
     3241 AGGAGGAACC CTTCATTTTT GAGAAGATCT TGGTGCCTGA GCTGGCCTCC CTCAGGATAG 
     3301 CTGTAATGGA AGAAGGCGGC AAATTTATTG GACATCGCAT CATCCCCATC AATGCCTTAC 
     3361 ATTCCGGATA CCACCATCTG TGCCTGCGCA GTGAGAGCAA CATGCCTCTC ACCATGCCTG 
     3421 CTCTCTTCGT CTTCCTGGAG ATGAAGGACT ATGTACCTGA CACGTGGGCA GATCTTACTG 
     3481 TGGCTCTTGC CAACCCCATC AAGTACTTCA GTGCCCATGA TAAGAAGTCT GTGAAGCTCA 
     3541 AAGAAGTGAC AGGAAGTCTG CCTGAGAAGC TCTTCTCTGG GATTCCTGTT GCCAGCCAGT 
     3601 CCAACGGGGC TCCAGTCTCC GCAGGCAATG GGTCAACAGC GCCTGGGACC AAGGCCAAGG 
     3661 AGGAAGCTAC GAAGGAAGTG GCAGAGCCAC AGACCACTAG CTTGGAAGAG CTGCGGGAGC 
     3721 TGAAGGGCGT GGTGAAGCTG CAGCGGAGAC ATGAGAAGGA GCTTCGAGAG CTGGAGCGGC 
     3781 GTGGAGCCCG GCGCTGGGAG GAGCTGCTGC AGCGTGGCGC TGCACAGCTA GCGGAGCTCC 
     3841 AGGACCCAGC AGCAAGCTGC AAGCTCCGCC CGGGCAAGGG CTCCCGCAAG AAGAGGATCG 
     3901 TGCCCTGTGA GGAGACCATC GTGGTGCCTA GGGAGGTCCT CGAGGGACCG GACCCCCGCG 
     3961 TGCAGGATCT GAAGGACAGG CTGGAGCAGG AGCTGCAGCA GCAAGGCGAG GAGCAGTACC 
     4021 GCTCTGTCCT CAAGCGTAAG GAGCAGCACG TGACCGAGCA AATCGCCAAG ATGATGGAGC 
     4081 TGGCCAGAGA GAAACAGGCT GCTGAACTCA AGAGCTTCAA GGAGACCTCG GAAACTGACA 
     4141 CCAAAGAGAT GAAGAAAAAA CTGGAGGCCA AAAGGCTGGA GCGGATCCAA GCCATGACCA 
     4201 AAGTCACCAC AGACAAGGTG GCCCAGGAGA GGCTCAAGAG AGAAATTAAC AACTCCCACA 
     4261 TCCAGGAGGT GGTGCAGGCT GTCAAGCAGA TGACAGAGAC CCTGGAGCGT CACCAGGAGA 
     4321 AGCTGGAAGA GAAGCAGACA GCCTGCCTGG AGCAGATCCA GGCAATGGAA AAGCAGTTCC 
     4381 AGGAGAAGGC GCTGGCAGAG TATGAGGCCA AGATGAAGGG CCTGGAGGCT GAAGTAAAGG 
     4441 AGTCAATGCG GGCTTGCTTC AAGGCCTGCT TCCCCACGGA AGCAGAAGAG AAGCCTGAGA 
     4501 GGCCCTGTGA GGCCTCTGAG GAGTCGTGTC CACAGGAGCC ACTTGTGAAC AAGACAGACA 
     4561 CTCAGGAGAG CCGCCTCTCC ACAGGAGCCA CTTGTGAACA AGACAGACAC TCAGGAGAGC 
     4621 CGCCTCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA 
     4681 AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT 
     4741 AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT 
     4801 GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT 
     4861 CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG 
     4921 CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG 
     4981 GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT 
     5041 CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT 
     5101 TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA 
     5161 ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC 
     5221 TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC 
     5281 ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC 
     5341 TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC 
     5401 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     5461 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     5521 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     5581 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     5641 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     5701 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     5761 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     5821 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     5881 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     5941 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     6001 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     6061 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     6121 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     6181 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     6241 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG 
     6301 CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG 
     6361 AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT 
     6421 GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT 
     6481 TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT 
     6541 CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA 
     6601 TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG 
     6661 TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA 
     6721 GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG 
     6781 AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC 
     6841 AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA 
     6901 GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC 
     6961 ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC 
     7021 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC 
     7081 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC 
     7141 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG 
     7201 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT 
     7261 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA 
     7321 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC 
     7381 AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT 
     7441 TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC 
     7501 GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT 
     7561 TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG 
     7621 AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG 
     7681 CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG 
     7741 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     7801 ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     7861 GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT 
     7921 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 
     7981 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 
     8041 GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 
     8101 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 
     8161 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 
     8221 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 
     8281 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 
     8341 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 
     8401 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 
     8461 AAAATTTAAC GCGAATTTT
//