LOCUS       RR202151                8964 bp    DNA     circular  SYN 
DEFINITION  Kdm6b (Myc-DDK-tagged ORF) - Rat jumonji domain containing 3 (Jmjd3), (10 ug)
ACCESSION   NM_001108829
VERSION     NM_001108829.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5121..5212 
                     /label="Myc-DDK"  
     misc_feature    5242..5827 
                     /label="PolyA signal"  
     misc_feature    5299..5318 
                     /label="Restriction Enzyme"  
     misc_feature    6103..6775 
                     /label="ColE1"  
     misc_feature    7205..7999 
                     /label="Kan/Neo"  
     misc_feature    8034..8336 
                     /label="SV40 ori"  
     misc_feature    8422..8899 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5103..5109 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5103 
                     /label="Kdm6b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCTGCC GCACTCTCAG GCCCCTCAGG AGAGGAAGGC CTCAGCCCTG 
     1081 GAGGCAAGCG CAGGAGAGGC TGCAGCTCTG AACAGGAGCA GCGGCACTCG CTGCCTCACC 
     1141 CTCATCCATA CCCAGCTCCT GCCTACACTG CTCATCCGCC CAGCCACCGG CTGGGCCCGG 
     1201 CCACACCCCT TGGTCCAGGT CCCCGACCCC CAGGAGCAGA GAGCCATGGC TGCCTGCCTG 
     1261 CCACCCGTCC CCCCGGAAGT GACCTTAGAG AGAGCAGAGT TCAGAGGTCG CGGATGGACT 
     1321 CCAGCGTTTC ACCAGCAGCA TCTACCGCCT GCGTGCCTTA CGCCCCTTCC CGGCCCCCTG 
     1381 GCCTCCCCGG CACCAGCAGC AGCAGCAGTA GCAGTAACAA CACTGGTCTT CGGGGTGTGG 
     1441 AGCCGAGCCC AGGCATTCCT GGCGCTGACC ATTACCAAAA CCCTGCGTTG GAGATAGCCC 
     1501 CTCACCAGGC CCGCCTGGGT CCCTCCGCAC ACAGCAGTCG GAAACCATTT TTGACAGCCC 
     1561 CTGCTGCCGC TCCCCACTTA TCCCTACCAT CCGGGACCCC TTCATCCCCT CCACCCCCAT 
     1621 GTCCTCGCCT CTTGCGCCCT CCACCACCCC CTGCTTGGAT GAAGGGCTCA GCCTGCCGCT 
     1681 CAGCTCGAGA GGATGGAGAG ATCTTAGGGG AGCTCTTCTT TGGTGCTGAG GGACCTCCCC 
     1741 GTCCTCCTCC CCCACCCCTT CCCCACCGTG ATGGCTTCTT GGGGCCTCCA AACCCCCGCT 
     1801 TTTCTGTGGG CACTCAGGAT TCGCATACCC CTCCCACTCC CCCAACCACC ACCAGCAGCA 
     1861 GCAGCAGCAG CAGCAGCAGC AACAGCCACA GCAGTAGTCC TACTGGGCCT GTGCCCTTCC 
     1921 CACCACCTCC CTATCTGGCC AGAAGTATAG ACCCCCTTCC CAGGCCATCT AGCCCAACAC 
     1981 TGAGCTCCCA GGACCCACCT CTTCCACCAC TGACTCTTGC CCTGCCTCCA GCCCCTCCCT 
     2041 CCTCCTGCCA CCAAAATACC TCAGGAAGCT TCAGGCGCTC GGAGAGCCCC CGGCCCAGGG 
     2101 TCTCCTTCCC AAAGACCCCC GAGGTGGGGC AGGGGCCACC TCCAGGCTCT CTGAGTAAAG 
     2161 CCCCCCAGCC TGTGCCACCT GGGGTTGGAG AGCTGCCTGC CCGAGGCCCG AGGCTCTTTG 
     2221 ATTTTCCACC AACTCCACTG GAGGACCAGT TTGAAGAGCC AGCTGAATTC AAGATCCTCC 
     2281 CTGATGGGCT AGCTAACATC ATGAAGATGC TGGATGAATC CATTAGAAAG GAGGAAGAGC 
     2341 AGCAGCAGCA AGAGGCAGGC GCGGTTCCCC CACCCCCACT CAAAGAGCCC TTTGCATCTC 
     2401 TACGGTCTTC ACTTCCCAGT GACACAGCCC CAGCTGCCAC CACTGCTGCT GCAACTCCCA 
     2461 CCACCACCAC CACCACCACC ACCCAAGAAG AGGAGAAGAA GCCACCACCA GCCCTGCCAC 
     2521 CACCACCGCC TCTAGCCAAG TTTCCTCCAC CACCCCAGCC CCCACCACCT CCACCAGCCA 
     2581 GCCCAGCCAG CCTGCTCAAA TCATTGGCCT CTGTTCTTGA GGGGCAAAAG TACTGTTATC 
     2641 GGGGGACTGG AGCAGCCGTT TCAACCAGGC CCGGGTCCGT GCCCGCCACT CAGTATTCCC 
     2701 CTAGTCCTGC ATCAGGTGCT ACCGCCCCAC CACCCACTTC AGTGGCCCCT AGCGCCCAGG 
     2761 GCTCCCCCAA GCCCTCTGTT TCCTCGTCAT CTCAGTTCTC TACCTCAGGT GGGCCTTGGG 
     2821 TCCGAGAGCG CAGGGCAGGT GAAGAGCCAG CACCAGGCCC CGTGACCCCT GCCCAGTTGC 
     2881 CCCCACCTCT GCCGCTGCCC CCTGCTCGTT CTGAGTCTGA GGTGCTAGAA GAAATCAGTC 
     2941 GGGCTTGTGA GACCCTTGTA GAGCGGGTGG GCCGGAGTGC CATCAACCCA GCAGACCCAG 
     3001 TGGACATAGC AGACCCAGTG GACAGTGGGA CTGAGCCACA GCCGCCTCCT GCACAGGCCA 
     3061 AGGAGGAGAG TGGGGGAGTG GCAGTGGCGG CAGCAGGTCC AGGTAGTGGT AAGCGTCGTC 
     3121 AGAAGGAGCA TCGGAGGCAC AGACGGACCT GTAGGGACAG TGTGGGTCGT AGGCCCCGTG 
     3181 AGGGGAGGGC CAAGGCCAAG GCCAAGGCTC CCAAAGAAAA AAGCCGAAGG GTGCTGGGGA 
     3241 ACTTGGACTT GCAGAGTGAA GAGATCCAGG GCCGGGAGAA GGCCCGGCCT GATGTAGGTG 
     3301 GGGCTTCCAA AGTCAAGACG CCCACAACTC CAGCGCCCCC ACCTGCTCCT GCCCCCTCTG 
     3361 CTCAGCCAAC ACCCCCATCA GCTCCTGTCC CTGGGAAGAA GACTCGAGAG GAAGCTCCAG 
     3421 GGCCTCCAGG TGTGAGCCGT GCAGATATGC TGAAGCTTCG GTCGCTTAGT GAGGGGCCAC 
     3481 CTAAGGAGCT GAAGATCAGG CTCATCAAGG TGGAGAGTGG GGACAAGGAG ACCTTCATCG 
     3541 CCTCTGAGGT AGAAGAGCGG CGGCTGCGCA TGGCAGACCT CACCATCAGC CACTGCGCTG 
     3601 CTGATGTCAT GCGTGCCAGC AAGAATGCCA AGGTGAAAGG GAAATTCCGA GAGTCCTACC 
     3661 TTTCCCCTGC CCAGTCTGTG AAACCGAAGA TCAACACTGA GGAGAAGCTG CCCCGGGAAA 
     3721 AACTCAACCC TCCTACACCC AGCATTTATT TGGAGAGCAA ACGAGACGCC TTCTCGCCGG 
     3781 TCCTGCTGCA GTTCTGTACA GATCCCCGGA ACCCCATCAC TGTCATCAGG GGCCTGGCAG 
     3841 GTTCACTTCG GCTCAACTTA GGCCTTTTTT CCACCAAGAC TCTGGTGGAG GCGAGCGGTG 
     3901 AACACACAGT GGAGGTCCGT ACCCAAGTGC AGCAGCCCTC AGACGAGAAC TGGGATCTGA 
     3961 CAGGTACCAG ACAGATCTGG CCCTGTGAGA GCTCCCGTTC ACACACCACC ATCGCTAAAT 
     4021 ATGCACAGTA CCAGGCTTCG TCCTTCCAGG AGTCACTGCA GGAGGAGAAA GAGAGTGAGG 
     4081 ATGAGGAATC CGAGGAACCA GACAGCACTA CAGGAACCTC TCCCAGCAGT GCACCAGACC 
     4141 CCAAGAACCA TCACATCATC AAGTTTGGCA CAAACATCGA CCTGTCTGAT GCCAAGAGGT 
     4201 GGAAGCCACA ACTACAGGAG CTGCTGAAAC TGCCTGCCTT CATGCGAGTA ACATCCACAG 
     4261 GCAACATGCT TAGCCATGTG GGACACACCA TCCTGGGCAT GAACACCGTG CAGCTATACA 
     4321 TGAAGGTTCC TGGCAGTCGA ACGCCAGGCC ACCAGGAGAA TAACAATTTT TGCTCTGTCA 
     4381 ATATCAACAT TGGCCCCGGG GACTGCGAGT GGTTCGCGGT ACATGAGCAC TATTGGGAGA 
     4441 CCATCAGCGC CTTCTGCGAT CGGCATGGTG TGGATTACTT GACTGGTTCC TGGTGGCCAA 
     4501 TCTTGGATGA CCTCTATGCG TCCAATATTC CTGTTTACCG CTTCGTGCAG CGCCCTGGAG 
     4561 ACCTTGTGTG GATTAATGCA GGGACTGTGC ATTGGGTGCA GGCTACTGGC TGGTGCAACA 
     4621 ACATTGCCTG GAACGTGGGG CCCCTCACCG CCTATCAATA CCAGCTGGCC CTGGAGCGAT 
     4681 ATGAGTGGAA CGAGGTGAAG AACGTCAAGT CCATTGTACC CATGATTCAT GTGTCCTGGA 
     4741 ACGTAGCTCG AACGGTCAAG ATCAGTGATC CTGACTTGTT CAAAATGATC AAGTTCTGCC 
     4801 TCCTGCAGTC AATGAAGCAC TGCCAGGTGC AGCGGGAGAG CCTGGTGCGG GCAGGGAAGA 
     4861 AAATCGCTTA CCAAGGCCGC GTCAAGGACG AGCCTGCCTA CTACTGCAAC GAATGCGATG 
     4921 TGGAGGTGTT CAACATCCTG TTCGTTACAA GTGAGAATGG CAGCCGAAAC ACGTACCTGG 
     4981 TGCACTGCGA GGGCTGTGCG CGCCGTCGCA GTGCGGGCCT ACAGGGCGTG GTGGTGCTAG 
     5041 AGCAGTACCG CACAGAGGAG CTGGCACAGG CCTATGATGC CTTCACACTG GCTCCTGCCA 
     5101 GCACGTCGCG AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5161 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5221 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5281 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5341 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5401 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5461 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5521 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5581 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5641 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5701 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5761 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5821 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5881 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5941 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6001 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6061 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6121 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6181 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6241 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6361 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6421 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6481 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6541 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6601 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6661 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6721 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6781 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6841 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6901 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6961 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7021 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7081 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7141 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7201 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7261 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7321 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7381 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7441 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7501 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7561 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7621 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7681 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7741 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7801 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7861 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7921 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7981 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8041 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8101 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8161 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8401 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8461 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8521 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8581 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8641 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8701 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8761 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8821 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8881 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8941 TAACAAAAAT TTAACGCGAA TTTT
//