LOCUS       RR202142                6787 bp    DNA     circular  SYN 
DEFINITION  Gpaa1 (Myc-DDK-tagged ORF) - Rat glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (Gpaa1), (10 ug)
ACCESSION   NM_001004240
VERSION     NM_001004240.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2944..3035 
                     /label="Myc-DDK"  
     misc_feature    3065..3650 
                     /label="PolyA signal"  
     misc_feature    3122..3141 
                     /label="Restriction Enzyme"  
     misc_feature    3926..4598 
                     /label="ColE1"  
     misc_feature    5028..5822 
                     /label="Kan/Neo"  
     misc_feature    5857..6159 
                     /label="SV40 ori"  
     misc_feature    6245..6722 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2926..2932 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2926 
                     /label="Gpaa1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTCTCCTG TCGGACCCGG TGCGCCGGCG CGCGCTCGCC CGCATCGTCT 
     1081 TGCGCCTCAA CACTCCTCTA TGTGTGCTGA GCTACGTAGC TGGCATCGCC TGGTTTTTGG 
     1141 CGCTGGCTTT TCCGCCGCTA ACCCAGCGCA CTTATATGTC TGAGAATGCC ATGGGCTCCA 
     1201 CCATGGTGGA GGAGCAGTTT GTAGGTGGAG ACCGTGCCCG CAACTTCGCC CGGGACTTTG 
     1261 CTGCCCACCG CAAGAAGCCA GGGGCTCTGC CAGTAGCCTG GCTGGAAAGG GCGATGCGAT 
     1321 CCGTAGGACT GGAGGTCTAC ACTCAGAGCT TCTCCCGCAA ACTACCCTTC CCGGATGAGA 
     1381 CCCATGAGCG CTATATGGTC TCGGGTACCA ATGTGTATGG CATCCTGCGG GCCCCACGTG 
     1441 CTGCCAGTAC CGAGTCCCTG GTACTCACTG TACCCTGTGG TCCAGACACC ACCAATAGCC 
     1501 AGGCTGTGGG GTTGTTGTTG GCACTCGCTG CCCATTTCCG AGGGCAGATT TACTGGGCCA 
     1561 AAGATATAAT TTTCCTGGTA ACAGAACATG ATCTTCTGGG CACTGAGGCT TGGCTTGAGG 
     1621 CCTACCATGA CATTAATGTT ACAGGCATAC AGTCATCTCC CCTGCAGGGC AGGGCTGGTG 
     1681 CCATCCAGGC CGCTGTGGCC CTGGAGCTGA GCAGTGATGT AGTCACCAGC CTCGACGTGA 
     1741 CCGTGGAGGG ACTCAACGGT CAGCTGCCCA ACCTTGATCT GCTCAACCTC TTCCAGACCT 
     1801 TCTGCCAGAA AGGGGGGCTG TTGTGCACAC TCCAGGGCAA GCTGCAGCCC CAGGACTGGA 
     1861 CATCACTGGA AGGGCCTCTG CAGGGCCTGC AGACACTGCT TCTCATGGTG CTGCGGCAGG 
     1921 CCTCTGGCCG GCCCCATGGC CCCCATGGCC TCTTCCTGCG CTACGGAGTG GAGGCCCTAA 
     1981 CCCTACGTGG CATCAACAGC TTCCGCCAAT ACAAGTATGA TTTGGTGGCA GTGGGCAAGG 
     2041 CTCTGGAGGG CATGTTCCGA AAGCTCAACC ACCTCCTGGA GCGCTTGCAC CAGTCGTTCT 
     2101 TCTTCTACCT ACTGCCCGCC CTCTCCCGCT TCGTGTCCAT TGGACTCTAC ATGCCCGCCA 
     2161 CAGGCTTCCT GCTCCTGGTC CTTGGACTCA AGGCTCTCGA GCTATGGATG CAGTTACACG 
     2221 AGGCTGGAGT GAACCCTGAG GAGGCTGGGA AGGCCCCTGG CCCCGGCTCT CCACCCTTGG 
     2281 CAACACAGAG CGTGAGCCTG GCCTCACTTA TGGCGCCCTT GCTGATCTCT CAGGCCATGG 
     2341 GTTTGGCCCT CTATTTCCTA CCTGTGCTGG GCCAACATGT AGCTGCCCAG CATTTCCCAG 
     2401 TGGCTGAAGC TGAGGCTGTC GTGTTGACAC TGCTAGCGAT TTATGTAGCC GGCCTAGCCC 
     2461 TGCCACACAA CACCCACCGA GTGGTCAGCT CACAGGTCCC AGACCGGGGC TGGATGGCGC 
     2521 TGAAACTGGT GGCCCTGATC TACTTGGCAC TGCAGCTGGG TTGCATTGCT CTCCTCAATT 
     2581 TCTCCCTGGG CTTCCTGCTG GCTGCCACCA TGGTACCTGC TGCTGCACTC ACGAAGCCCC 
     2641 ACGGACCCCG GACACTCTAT GCTGCCCTGC TGGTAGTGAC CAGCCCAGCA GTCACACTCT 
     2701 TCGGTAGCCT ATTCCTGTGG CGGGAGCTCC AGGAGGTACC ACTGTCTCTG GCAGAGGGTT 
     2761 GGCAGCTCTT CCTGACTGCC TTGGCCCAGG GTGTGCTGGA ACACTACACC TATGGTGCCC 
     2821 TCCTCTTCCC AATACTGGCC TTGGGTCTGT ATCCTTGCTG GCTGCTCTTC TGGAATGTTC 
     2881 TCTTCTGGAA GGTATCCTTG CTGGCTGCTC TTCTGGAATG TTCTCTTCTG GAAGACGCGT 
     2941 ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG 
     3001 GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG 
     3061 AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT 
     3121 TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA 
     3181 CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT 
     3241 TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC 
     3301 ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC 
     3361 TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG 
     3421 GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT 
     3481 CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC 
     3541 TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT 
     3601 ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG 
     3661 CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT 
     3721 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 
     3781 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 
     3841 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 
     3901 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 
     3961 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 
     4021 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 
     4081 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 
     4141 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 
     4201 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 
     4261 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 
     4321 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 
     4381 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     4441 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     4501 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     4561 AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT 
     4621 TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA 
     4681 CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG 
     4741 ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA 
     4801 TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC 
     4861 CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG 
     4921 CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT 
     4981 CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA 
     5041 GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC 
     5101 GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT 
     5161 AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC 
     5221 AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC 
     5281 GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG 
     5341 CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG 
     5401 TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT 
     5461 ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA 
     5521 TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT 
     5581 GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC 
     5641 TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG 
     5701 GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC 
     5761 CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC 
     5821 TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA 
     5881 GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG 
     5941 CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG 
     6001 GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT 
     6061 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG 
     6121 ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC 
     6181 ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA 
     6241 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 
     6301 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC 
     6361 GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC 
     6421 ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT 
     6481 CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC 
     6541 TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC 
     6601 GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT 
     6661 CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 
     6721 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 
     6781 GAATTTT
//