LOCUS       RR202098                8490 bp    DNA     circular  SYN 
DEFINITION  Slk (Myc-DDK-tagged ORF) - Rat STE20-like kinase (yeast) (Slk), (10 ug)
ACCESSION   NM_019349
VERSION     NM_019349.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="Slk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTTCTTC AATTTCCGTA AGATCTTCAA GTTGGGGAGC GAGAAGAAGA 
     1081 AGAAACAGTA CGAACACGTG AAGAGAGACC TGAACCCCGA GGAGTTTTGG GAGATTATCG 
     1141 GAGAGCTGGG CGACGGAGCC TTTGGGAAAG TGTATAAGGC CCAGAATAAG GAGACCAATG 
     1201 TTTTAGCTGC TGCAAAGGTG ATTGACACCA AATCTGAAGA AGAACTTGAA GATTATATGG 
     1261 TTGAGATTGA CATATTAGCA TCTTGTGATC ACCCAAACAT AGTCAAGCTT CTAGATGCCT 
     1321 TCTATTATGA GAACAATCTT TGGATCCTCA TTGAATTTTG TGCAGGGGGA GCAGTAGATG 
     1381 CCGTGATGCT TGAACTTGAG AGACCATTAA CTGAGTCCCA AATTCAAGTA GTTTGCAAGC 
     1441 AGACATTAGA GGCGTTGAAT TACCTACATG ACAATAAAAT CATCCACAGA GATCTAAAAG 
     1501 CTGGCAATAT TCTCTTTACC TTAGATGGAG ACATTAAATT AGCGGATTTT GGAGTGTCAG 
     1561 CTAAGAATAC CAGGACAATT CAAAGGAGGG ATTCATTTAT TGGCACACCG TATTGGATGG 
     1621 CTCCTGAAGT CGTCATGTGT GAGACATCGA AGGACAGACC CTATGACTAC AAAGCGGACG 
     1681 TTTGGTCCCT GGGAATCACT TTAATAGAAA TGGCTGAGAT AGAGCCGCCT CATCATGAGC 
     1741 TAAACCCAAT GCGAGTGCTG CTGAAAATAG CAAAGTCGGA GCCCCCCACA CTAGCACAGC 
     1801 CATCCAGATG GTCTTCAAAT TTTAAGGACT TTCTAAAGAA ATGCTTGGAA AAGAATGTGG 
     1861 ATGCGCGGTG GACCACATCC CAGCTGTTAC AGCATCCCTT TGTTACCGTT GATTCCAACA 
     1921 AACCAGTCCG AGAATTGATT GCTGAGGCGA AGGCTGAAGT AACAGAAGAA GTTGAAGATG 
     1981 GCAAAGAGGA AGATGACGAT GATGAGACAG AGAGTGCTCT GCCAATACCT GCGAATAAAC 
     2041 GCGCCTCCTC TGACCTCAGC ATTGCCAGCT CTGAGGAAGA TAAGCTTTCA CAAAATGCTT 
     2101 GTATTTTGGA ATCTGTGTCA GAAAGAACAG AACACAATAC TTCTGGGGAT AAATTTAGCA 
     2161 ACAAAGTTCT TAGTGAGAAG CCTACTCCTG AGGGACCTGA GAAGACTGTG GATGTGGATG 
     2221 GGCCTGCAAA TGATGTTAAC TTAGAAACTG TGGCTGAACC AAATGACCAA GCGGTAGGAT 
     2281 TCCATGAGAA TGGGAGAGAG AAGAAAAGAC CCCAACTGGA AAGTCAACCA GATACAGAAG 
     2341 ACCAACAAAC TGTGGATGTT AATTTAGTCG GTGAAGGAAA TGACAGCAAT ATAGTAATTT 
     2401 TAGAAACAAA CACTGATTGT CTGAAACCAG AGGAAGACAG AAATGAAGAA AACCAAGAGA 
     2461 TAATTGAGAA TAAACTTACA CAATCTGAAG AAATTAAAGA TATACATATT CAAACAATGG 
     2521 ACTTAGTTTC TCAAGAAACT GGAGAAAAAG AGGCAGATTT TCAGGCAATT GACAATGAAG 
     2581 TTGGGTTTAC AAAGGAAGAA ACCCAAGAGA AATTGGGAAA AGATGATAAA ACTCACAAAG 
     2641 TTGTGATCAG TGATATAACC AGTGAGGTGG GAACAGACGA GCCTCCAGGT GATACCCAGA 
     2701 AGTCTGCTGA GCAGAGCCAG GATGCAGAGG GTGGGGCTGG GGAAGAAGCC CCGGAGCCTG 
     2761 CCCAGACACT AACAGAGAAG GCCACTGAGG GCCCTGAGGC TCATGGTGCT GAGGAAGAGC 
     2821 CTCGTAGTGG AGAGAGAGTA GAGGATAAGC AGCTAGAGCA GCAGTCTGCA GTGTGTGAAG 
     2881 GTGAGGGACA GGTAACCAGC ACTTCAGAGA GCACACGGGC AACCACGGAG GAACCAGAGA 
     2941 CTGACGAAGT TGACCAAGTC AGCGAGTCAA ATAGCATTGA GGAACTAGAG AGACTTGGAG 
     3001 TTACTGGTGC TGAGGAACAG GCTCTAGGGA GCAAAGGGGA GGCTGCTACG GAGCTAGATT 
     3061 TGGAGAGAGA AGAAAATGCA CAGGAACTGC CAGTTAAAGC AGAGCCCCAA GCTCCTGCAG 
     3121 CATCGCAGGC CAGCGAACCT CCCCCTGTCT TAATACCCAG TATTAATATT CATTCGGAAA 
     3181 ACACAGAAAA TAAAGGAGAA ATGGGTGCTT TGCCAAAACC TGAAACCATA CTGCCACCAG 
     3241 AGCCTGAAAA TGGAAAGGGA AATGACACGG ACTCAGGGAC TGGGTCCACT GTGGAGAACA 
     3301 GCAGCAGTGA CCTGAACTTG TCCATCTCTA GCTTCCTGAG CAAAACCAAG GACAGTGGCT 
     3361 CGGTGTCTCT GCAGGAGACA AGAAGACAGA AGAAAACATT GAAGAAAACA CGTAAATTTA 
     3421 TTGTTGATGG AGTAGAAGTG AGTGTGACAA CATCAAAGAT AGTTACAGAC AGCGACTCTA 
     3481 AAACCGAGGA ATTGAGGTTT CTCAGGCGTC AGGAACTTCG AGAGCTAAGG CTTCTCCAGA 
     3541 AGGAAGAGCA GAAAGCCCAG CAGCAGCTCA ACGGGAAATT GCAGCAGCAG CGCGAGCAGA 
     3601 TCTTCAGGCG CTTTGAGCAG GAGATGCTGA GTAAGAAGCG ACAATATGAC CAAGAAATTG 
     3661 AGAATCTAGA GAAGCAGCAG AAACAGACAA TTGAACGACT AGAACAAGAA CACACAAACC 
     3721 GCCTGAGAGA CGAAGCTAAG CGCATCAAAG GAGAGCAGGA GAAGGAGCTG TCCAAATTCC 
     3781 AGAATATGCT AAGAAACCGA AAGAAGGAGG AACAAGAATT TGTTCAGAAG CAACAGCAAG 
     3841 AGTTAGACGG CGCTCTGAAA AAGATCATCC AGCAGCAGAA GGCAGAGTTG GCCAATATTG 
     3901 AGAGAGAGTG CTTGAATAAC AAGCAGCAGC TCCTGAGAGC CCGAGAAGCT GCAATTTGGG 
     3961 AGCTTGAAGA ACGACATTTA CAAGAAAAGC ACCAGCTGCT TAAACAGCAA CTTAAAGATC 
     4021 AGTATTTCAT ACAGAGACAT CAGCTGCTTA AACGCCATGA GAAGGAAACA GAACAAATGC 
     4081 AGCGTTACAA TCAACGACTT ATTGAAGAAT TGAAGAACAG ACAGACTCAG GAACGAGCTA 
     4141 GACTGCCCAA GATTCAGAGA AGTGAAGCCA AGACACGAAT GGCCATGTTT AAAAAAAGTT 
     4201 TGAGGATTAA CTCAACAGCT ACACCAGACC AGGACCGTGA AAAAATTAAA CAGTTTGCTG 
     4261 CACAGGAAGA AAAGAGACAG AAAAATGAGA GAATGGCTCA GCATCAAAAA CATGAGAGTC 
     4321 AGATGCGGGA CCTTCAGTTG CAGTGTGAAG CCAACGTCCG AGAGCTGCAC CAGCTGCAGA 
     4381 ATGAAAAATG CCACCTGTTG GTTGAACATG AGACTCAGAA ACTGAAGGAG TTAGATGAGG 
     4441 AGCACAGCCA AGAGCTCAAG GAGTGGAGAG AGAAGCTGAG GCCCAGGAAG AAGACACTGG 
     4501 AGGAAGAGTT TGCCAGGAAA CTTCAGGAAC AGGAAGTGTT CTTTAAAATG ACCGGGGAGT 
     4561 CCGAATGCCT CAACCCCTCA GCGCAGAGCC GGATCTCCAA ATTCTACCCT ATTCCCACCT 
     4621 TACATTCCAC TGGGTCAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//