LOCUS       RR202059                8757 bp    DNA     circular  SYN 
DEFINITION  Synj1 (Myc-DDK-tagged ORF) - Rat synaptojanin 1 (Synj1), (10 ug)
ACCESSION   NM_053476
VERSION     NM_053476.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4914..5005 
                     /label="Myc-DDK"  
     misc_feature    5035..5620 
                     /label="PolyA signal"  
     misc_feature    5092..5111 
                     /label="Restriction Enzyme"  
     misc_feature    5896..6568 
                     /label="ColE1"  
     misc_feature    6998..7792 
                     /label="Kan/Neo"  
     misc_feature    7827..8129 
                     /label="SV40 ori"  
     misc_feature    8215..8692 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4896..4902 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4896 
                     /label="Synj1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTTCAGC AAAGGATTTC GAATCTACCA CAAATTGGAT CCCCCACCGT 
     1081 TCAGCCTCAT AGTGGAAACT AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCCG 
     1141 TGGCAGTGCT CTCATCTGCA GAAAAGGAGG CCATTAAAGG CACATATGCC AAAGTGCTGG 
     1201 ATGCATATGG ACTTTTGGGA GTTTTACGAT TAAATCTTGG TGATACCATG TTGCATTATC 
     1261 TGGTCCTAGT CACTGGATGC ATGTCTGTTG GGAAAATTCA AGAATCTGAA GTCTTCCGAG 
     1321 TTACTTCCAC TGAGTTTATA TCACTTCGAG TTGATGCTTC AGATGAGGAC CGCATTTCAG 
     1381 AAGTACGAAA GGTTTTGAAC TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCTGGAGTCA 
     1441 GCTTAGATCT GAGTCTGAAC GCACATCGAA GCATGCAGGA GCACACAACT GACAATAGGT 
     1501 TTTTCTGGAA TCAGTCTTTG CATCTGCATC TCAAGCACTA TGGTGTGAAT TGTGATGACT 
     1561 GGTTGTTACG GCTCATGTGT GGAGGAGTAG AGATTAGAAC AATTTATGCT GCCCATAAAC 
     1621 AGGCAAAAGC TTGCCTCATT TCAAGACTAA GCTGTGAGCG AGCTGGGACA AGGTTCAACG 
     1681 TCCGGGGAAC AAATGATGAC GGTCATGTTG CCAACTTTGT AGAGACAGAG CAGGTTATAT 
     1741 ACTTAGATGA CTGTGTCTCC TCCTTCATAC AAATCCGAGG ATCTGTTCCG TTGTTCTGGG 
     1801 AGCAACCAGG GTTGCAAGTG GGATCTCATC GTGTTCGAAT GTCAAGGGGA TTTGAAGCCA 
     1861 ATGCGCCTGC CTTTGACAGG CATTTCCGAA CCCTTAAGGA CTTATATGGT AAACAAATAG 
     1921 TAGTAAATCT GCTTGGATCT AAGGAGGGTG AGCATATGCT GAGCAAGGCC TTCCAGAGTC 
     1981 ACTTGAAAGC CTCTGAACAC GCTTCTGATA TCCACATGGT GTCCTTTGAT TATCATCAAA 
     2041 TGGTTAAAGG AGGAAAGGCA GAAAAATTGC ACAGTGTTCT CAAACCACAA GTCCAGAAGT 
     2101 TTCTAGATTA TGGATTTTTT TATTTCGATG GCAGTGCAGT TCAAAGGTGC CAGAGTGGTA 
     2161 CAGTTCGGAC AAACTGCCTG GACTGTCTGG ATAGAACAAA CAGTGTGCAG GCGTTCCTTG 
     2221 GCTTAGAGAT GCTAGCTAAA CAGCTGGAAG CTCTGGGCTT GGCTGAAAAG CCTCAGTTGG 
     2281 TGACTCGCTT TCAAGAAGTT TTCCGGTCCA TGTGGTCTGT GAATGGCGAT TCAATCAGTA 
     2341 AAATATATGC AGGCACCGGA GCCCTGGAAG GGAAGGCCAA GTTAAAAGAT GGTGCGCGAT 
     2401 CTGTCACCAG AACCATTCAG AATAACTTCT TCGACAGCTC CAAGCAAGAA GCCATTGATG 
     2461 TTCTGCTTCT GGGAAACACT CTGAACAGCG ACTTAGCCGA CAAAGCCCGG GCACTTCTAA 
     2521 CTACTGGGAG TTTGCGTGTG TCTGAACAGA CATTACAGTC AGCATCTTCC AAAGTCTTAA 
     2581 AGAACATGTG TGAGAACTTC TACAAGTACT CAAAGCCCAA GAAGATCCGA GTGTGTGTTG 
     2641 GAACCTGGAA TGTGAACGGC GGGAAACAGT TCCGCAGCAT AGCATTCAAG AACCAGACCC 
     2701 TCACCGACTG GCTTCTTGAT GCACCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAGA 
     2761 GAAGTAAGCC AACTGATATA TTTGCAATTG GCTTTGAAGA AATGGTGGAG CTGAATGCTG 
     2821 GGAACATTGT GAATGCAAGC ACAACAAACC AGAAGCTGTG GGCAGTGGAA CTGCAGAAGA 
     2881 CCATCTCCAG AGACAACAAG TATGTTCTCC TGGCCTCGGA GCAGTTGGTG GGTGTCTGCC 
     2941 TGTTTGTCTT TATCAGACCG CAGCATGCTC CCTTTATCAG GGATGTTGCA GTCGATACTG 
     3001 TGAAAACTGG GATGGGAGGC GCGACTGGAA ATAAGGGCGC TGTTGCAATC CGGATGCTCT 
     3061 TCCACACCAC CAGCCTTTGC TTTGTCTGCA GTCACTTTGC TGCAGGCCAG TCCCAAGTCA 
     3121 AAGAGAGAAA TGAAGATTTC GTAGAAATAG CACGAAAATT GAGTTTCCCC ATGGGAAGAA 
     3181 TGCTCTTCTC CCATGATTAC GTATTCTGGT GTGGTGATTT CAACTATCGA ATCGATCTTC 
     3241 CTAATGAAGA AGTTAAAGAG CTCATAAGAC AGCAAAATTG GGATTCTCTT ATAGCTGGAG 
     3301 ATCAACTCAT CAATCAGAAA AATGCAGGAC AGATCTTTAG AGGATTTTTA GAGGGAAAAG 
     3361 TAACGTTTGC TCCAACCTAT AAATATGACT TGTTTTCCGA AGACTATGAC ACCAGTGAAA 
     3421 AGTGCCGCAC CCCTGCTTGG ACAGACCGAG TCCTCTGGAG AAGGAGGAAG TGGCCTTTTG 
     3481 ACAGATCAGC TGAAGACTTA GATCTTCTGA ATGCTAGTTT CCAAGATGAA AGTAAAATCC 
     3541 TGTATACATG GACTCCTGGC ACCTTGCTGC ACTATGGAAG AGCCGAGCTG AAGACTTCTG 
     3601 ACCACAGGCC CGTTGTCGCC CTGATCGATA TAGATATATT TGAAGTTGAA GCTGAAGAGA 
     3661 GACAAAAAAT TTATAAAGAA GTTATTGCAG TCCAGGGCCC ACCGGACGGC ACAGTACTGG 
     3721 TCTCAATCAA AAGTTCTGCA CAAGAGAATA CTTTCTTTGA CGATGCTTTA ATCGATGAGC 
     3781 TTCTGCAGCA GTTTGCACAC TTCGGCGAAG TTATACTTAT AAGATTCGTA GAAGATAAAA 
     3841 TGTGGGTTAC ATTTTTGGAG GGGAGCTCTG CCTTGAATGT TCTGAGCCTA AATGGTAAAG 
     3901 AGTTATTAAA TCGGACTATA ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG 
     3961 AAGAAGAGAT GAGTTTAGAG AAAATCAGTG TCACATTGCC CTCATCAACC AGTTCCACCC 
     4021 TGCTCGGTGA AGATGCAGAG GTCTCAGCGG ACTTTGACAT GGAAGGTGAT GTCGACGACT 
     4081 ACAGTGCTGA GGTGGAGGAG CTTCTTCCTC AGCACCTCCA GCCATCCTCC AGCTCTGGCC 
     4141 TCGGCACCTC TCCAAGCTCT TCGCCCCGGA CCAGTCCCTG CCAGTCACCC ACAGCACCAG 
     4201 AGTACTCCGC ACCTTCTCTT CCCATCAGAC CTAGTCGAGC ACCATCAAGA ACTCCAGGAC 
     4261 CTCTAAGTTC ACAGGGTGCT CCAGTTGACA CCCAGCCAGC AGCCCAGAAG GAGTCTTCCC 
     4321 AGACCATAGA GCCCAAGAGG CCACCCCCTC CCCGCCCAGT TGCTCCTCCA GCACGTCCTG 
     4381 CCCCACCACA GAGGCCACCT CCACCTTCAG GGGCTAGGAG TCCGGCACCT GCTAGAAAAG 
     4441 AATTTGGAGC ACCCAAAAGC CCGGGAACAG CACGGAAAGA TAATATAGGG CGTAATCAGC 
     4501 CTTCCCCTCA AGCCGGACTT GCAGGCCCAG GACCTTCTGG ATATGGTGCA GCTAGACCGA 
     4561 CAATTCCAGC TCGTGCTGGA GTGATCAGTG CCCCTCAGAG CCAGGCTCGG GTGTCTGCTG 
     4621 GAAGGCTGAC TCCTGAAAGC CAAAGCAAAC CCTTGGAGAC ATCGAAAGGT CCTGCTGTCC 
     4681 TTCCAGAACC ACTGAAGCCT CAGGCTGCAT TTCCTCCGCA GCCTTCTCTG CCCACACCTG 
     4741 CTCAAAAGTT ACAGGACCCA CTTGTCCCCA TAGCAGCACC TATGCCTCCC TCCATTCCCC 
     4801 AATCGAATTT GGAAACTCCA CCCCTGCCCC CACCTCGGAG CAGGTCATCT CAAAGCTTGC 
     4861 CTTCAGACTC CTCACCACAG CTACAACAGG AGCAACCAAC AGGGACGCGT ACGCGGCCGC 
     4921 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4981 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5041 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5101 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5161 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5221 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5281 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5341 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5401 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5461 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5521 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5581 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5641 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5701 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5761 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5821 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5881 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5941 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6001 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6061 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6121 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6181 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6241 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6301 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6361 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6421 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6481 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6541 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6601 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6661 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6721 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6781 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6841 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6901 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6961 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7021 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7081 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7141 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7201 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7261 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7321 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7381 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7441 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7501 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7561 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7621 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7681 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7741 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7801 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7861 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7921 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7981 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8041 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8101 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8161 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8221 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8281 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8341 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8401 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8461 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8521 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8581 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8641 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8701 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//