LOCUS       RR201958                7663 bp    DNA     circular  SYN 
DEFINITION  Gria1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, AMPA 1 (Gria1), (10 ug)
ACCESSION   NM_031608
VERSION     NM_031608.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3820..3911 
                     /label="Myc-DDK"  
     misc_feature    3941..4526 
                     /label="PolyA signal"  
     misc_feature    3998..4017 
                     /label="Restriction Enzyme"  
     misc_feature    4802..5474 
                     /label="ColE1"  
     misc_feature    5904..6698 
                     /label="Kan/Neo"  
     misc_feature    6733..7035 
                     /label="SV40 ori"  
     misc_feature    7121..7598 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3802..3808 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3802 
                     /label="Gria1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGTACATC TTTGCCTTTT TCTGCACCGG TTTTCTAGGT GCGGTTGTGG 
     1081 GTGCCAATTT CCCCAACAAT ATCCAGATAG GGGGGTTATT TCCAAACCAA CAATCACAGG 
     1141 AACATGCGGC TTTTAGGTTT GCTTTGTCAC AACTCACGGA GCCCCCCAAG CTGCTTCCCC 
     1201 AGATCGATAT TGTGAACATC AGCGACAGCT TTGAGATGAC TTACCGTTTC TGTTCCCAGT 
     1261 TCTCCAAAGG AGTCTATGCC ATCTTTGGAT TTTATGAACG AAGGACTGTC AACATGCTGA 
     1321 CCTCCTTCTG TGGGGCCCTC CATGTGTGCT TCATTACTCC AAGTTTTCCT GTTGACACAT 
     1381 CCAATCAATT TGTCCTTCAG CTACGCCCGG AACTACAGGA AGCTCTCATT AGCATTATCG 
     1441 ACCATTACAA GTGGCAAACC TTTGTCTACA TTTATGATGC TGACCGGGGC CTGTCAGTCC 
     1501 TGCAGAGAGT CTTGGATACA GCCGCAGAGA AGAACTGGCA GGTAACGGCT GTCAACATTC 
     1561 TGACAACCAC CGAGGAAGGA TACCGGATGC TCTTTCAGGA CCTGGAGAAG AAAAAGGAGA 
     1621 GGCTGGTGGT GGTTGACTGT GAATCAGAAC GCCTCAACGC CATCCTGGGC CAGATCGTGA 
     1681 AGCTAGAAAA GAATGGCATC GGGTACCACT ACATCCTCGC CAATCTGGGC TTCATGGACA 
     1741 TTGACTTAAA TAAGTTCAAG GAGAGCGGAG CCAATGTGAC AGGTTTCCAG CTGGTGAACT 
     1801 ACACAGACAC GATCCCAGCC AGAATCATGC AGCAATGGAG GACAAGTGAC TCCCGAGACC 
     1861 ATACCAGGGT GGACTGGAAG AGGCCAAAGT ACACTTCTGC TCTCACCTAT GATGGTGTCA 
     1921 AGGTGATGGC TGAGGCCTTC CAAAGCCTGC GGAGGCAGAG GATTGACATA TCCCGCCGGG 
     1981 GGAATGCTGG GGACTGTCTG GCTAACCCAG CTGTGCCCTG GGGTCAAGGG ATCGACATCC 
     2041 AGAGAGCCCT GCAGCAGGTG CGCTTCGAAG GTTTGACAGG AAATGTGCAG TTCAACGAGA 
     2101 AAGGGCGCCG GACCAATTAC ACCCTCCACG TGATCGAAAT GAAACATGAT GGAATCCGAA 
     2161 AGATTGGTTA CTGGAATGAA GACGATAAAT TTGTCCCCGC AGCCACCGAC GCTCAGGCTG 
     2221 GAGGGGACAA CTCAAGCGTC CAGAATAGGA CCTACATCGT CACTACTATC CTCGAAGATC 
     2281 CTTACGTGAT GCTTAAAAAG AATGCCAACC AGTTTGAGGG CAATGACCGC TATGAGGGCT 
     2341 ACTGTGTGGA GCTGGCTGCA GAGATCGCCA AGCACGTGGG CTACTCCTAC CGACTTGAGA 
     2401 TTGTCAGCGA CGGCAAATAT GGAGCCCGGG ATCCCGACAC AAAGGCTTGG AATGGCATGG 
     2461 TGGGAGAACT GGTCTATGGA AGAGCAGACG TGGCTGTGGC TCCCTTGACC ATAACCTTGG 
     2521 TCCGGGAGGA AGTCATCGAC TTCTCCAAGC CATTCATGAG TTTGGGAATC TCCATTATGA 
     2581 TTAAGAAGCC ACAGAAGTCC AAGCCAGGTG TCTTCTCCTT TCTTGACCCT TTGGCCTATG 
     2641 AGATCTGGAT GTGTATAGTG TTTGCCTACA TTGGAGTGAG CGTCGTCCTC TTCCTGGTCA 
     2701 GCCGTTTCAG CCCCTACGAA TGGCACAGCG AAGAGTTTGA AGAGGGACGA GACCAGACAA 
     2761 CCAGTGACCA GTCAAATGAG TTTGGCATAT TCAACAGCCT GTGGTTCTCC CTGGGGGCCT 
     2821 TCATGCAGCA AGGATGTGAC ATTTCCCCCA GGTCCCTGTC CGGACGCATC GTCGGCGGCG 
     2881 TCTGGTGGTT CTTCACTTTG ATCATCATCT CCTCGTACAC AGCCAACCTG GCTGCCTTCC 
     2941 TGACTGTGGA GAGGATGGTG TCTCCCATTG AGAGTGCAGA GGACCTGGCA AAGCAGACGG 
     3001 AAATTGCTTA TGGGACATTG GAAGCAGGCT CCACTAAGGA GTTCTTCAGG AGATCTAAAA 
     3061 TCGCTGTGTT TGAGAAGATG TGGACATACA TGAAGTCTGC AGAACCATCC GTGTTTGTTC 
     3121 GGACCACAGA GGAAGGCATG ATCAGAGTGA GAAAATCTAA AGGCAAATAC GCCTACCTCC 
     3181 TGGAGTCCAC CATGAATGAG TATATTGAGC AACGAAAGCC CTGTGACACC ATGAAAGTGG 
     3241 GAGGTAACTT GGATTCCAAA GGCTATGGCA TTGCGACACC CAAGGGGTCC GCCCTGAGAA 
     3301 ATCCAGTAAA CCTGGCAGTG TTAAAACTGA ACGAGCAGGG GCTTTTGGAC AAATTGAAAA 
     3361 ACAAATGGTG GTACGACAAG GGCGAGTGCG GCAGCGGGGG AGGTGACTCC AAGGACAAGA 
     3421 CCAGCGCTTT GAGCCTCAGC AATGTGGCAG GCGTGTTCTA CATCCTGATT GGAGGGCTGG 
     3481 GACTGGCCAT GCTGGTTGCC TTAATCGAGT TCTGCTACAA ATCCCGTAGC GAGTCGAAGC 
     3541 GGATGAAGGG TTTCTGTTTG ATCCCACAGC AATCCATCAA TGAAGCCATA CGGACATCGA 
     3601 CCCTCCCCCG GAACAGTGGG GCAGGAGCCA GCGGAGGAGG CGGCAGTGGA GAGAATGGCC 
     3661 GGGTGGTCAG CCAGGACTTC CCCAAGTCCA TGCAATCCAT TCCCTGCATG AGTCACAGTT 
     3721 CAGGGATGCC CTTGGGAGCC ACAGGATTGC ATGCAATCCA TTCCCTGCAT GAGTCACAGT 
     3781 TCAGGGATGC CCTTGGGAGC CACAGGATTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC 
     3841 ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG 
     3901 GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG 
     3961 TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT 
     4021 TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT 
     4081 GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG 
     4141 CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC 
     4201 CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC 
     4261 ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT 
     4321 GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT 
     4381 GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT 
     4441 ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG 
     4501 ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC 
     4561 TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     4621 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     4681 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     4741 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     4801 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     4861 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     4921 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     4981 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     5041 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     5101 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     5161 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     5221 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 
     5281 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     5341 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     5401 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     5461 AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC 
     5521 CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG 
     5581 GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA 
     5641 ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT 
     5701 CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA 
     5761 GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC 
     5821 CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG 
     5881 CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA 
     5941 GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC 
     6001 CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG 
     6061 ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT 
     6121 GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT 
     6181 CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC 
     6241 ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC 
     6301 TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC 
     6361 CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC 
     6421 TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA 
     6481 AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG 
     6541 GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA 
     6601 CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC 
     6661 CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC 
     6721 TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT 
     6781 ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA 
     6841 GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG 
     6901 TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG 
     6961 CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA 
     7021 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA 
     7081 GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 
     7141 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 
     7201 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC 
     7261 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 
     7321 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 
     7381 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 
     7441 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 
     7501 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 
     7561 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 
     7621 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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