LOCUS       RR201942                7515 bp    DNA     circular  SYN 
DEFINITION  Dnmt3b (Myc-DDK-tagged ORF) - Rat DNA methyltransferase 3B (Dnmt3b), (10 ug)
ACCESSION   NM_001003959
VERSION     NM_001003959.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3672..3763 
                     /label="Myc-DDK"  
     misc_feature    3793..4378 
                     /label="PolyA signal"  
     misc_feature    3850..3869 
                     /label="Restriction Enzyme"  
     misc_feature    4654..5326 
                     /label="ColE1"  
     misc_feature    5756..6550 
                     /label="Kan/Neo"  
     misc_feature    6585..6887 
                     /label="SV40 ori"  
     misc_feature    6973..7450 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3654..3660 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3654 
                     /label="Dnmt3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGGAGAC AGCAGACATC TTAATGAAGA AGAGGGTGCT AGTGGGTATG 
     1081 AGGACTGTAT CATTGTTAAT GGGAACTGTA GTGACCAGTC CTCGGACACG AAGGATGCTC 
     1141 CCTCACCCCC AGTCTTGGAG GCAATCTGCA CAGAGCCAGT CTGTACACCA GAGACCAGAG 
     1201 GCCGCAGATC AAGCTCACGG CTGTCTAAGA GGGAGGTCTC CAGCCTTCTG AATTACACGC 
     1261 AGGACGTGGT AGGAGATGGA GATGGTGAAG CGGATGATGG AGATGGCTCT GATATTCTCA 
     1321 TGATGCCAAA GCTCACCCGA GAGACCAAGG ATGCCAGGAC TCCCTCTGAA AGCCCAGCCG 
     1381 TCCGAACCCG AAACAGCAAC AGTATCTCCA GCCTGGAGAG GCAAAGAACC TCCCCCAGAA 
     1441 TCACGCGAGG CCGGCAGGGC CGCTACCACG TTCAGGAGTA CCCCGTGGAG TTCCCAGCTA 
     1501 CCAAGTCTCG GAGGCGGCGA GCATCGTCTT CAGCAAGCAC GCCATGGTCA TCCCCTGCCA 
     1561 GCATCGAGCT CATGGAAGAT GTGACACCTA AGAGCAGCAG TACGCCATCG GTTGACTTGA 
     1621 GCCAGGACGG CCCTCAGGAG GGCATGGATG CCACACAGGT GGATGCGGAG AGCAGGGATG 
     1681 GTGACAGCAC TGAGTATCAG GATGACAAGG AGTTTGGAAT AGGTGACCTT GTGTGGGGAA 
     1741 AGATCAAGGG CTTCTCCTGG TGGCCTGCCA TGGTGGTGTC CTGGAAAGCC ACCTCCAAGC 
     1801 GCCAGGCCAT GCCCGGAATG CGCTGGGTAC AGTGGTTTGG CGATGGCAAG TTTTCTGAGA 
     1861 TCGCTGCTGA CAAGCTGGTG GCTCTGGGTC TGTTCAGCCA GCACTTTAAT CTGGCCACCT 
     1921 TCAATAAGCT GGTTTCTTAT AGGAAGGCCA TGTACCACAC TCTGGAGAAA GCCATGGTGC 
     1981 GAGCTGGCAA GACCTTCCCC AGCCGCCCTG GAGACTCACT GGAGGACCAG CTGAAGCCCA 
     2041 TGCTGGAGTG GGCCCACGGG GGCTTCAAGC CCACTGGGAT CGAGGGCCTC AAACCCAACA 
     2101 ACAAGCAACC AGAGGTTCAT AAGTCGAAGG TGCGTCGTTC AGGCAGTAGG AACTTAGAAG 
     2161 CCAGGAGACG CGAGAACAAA AGTCGAAGAC GCACAACCAT TGACTTTGCC GCTTCTGAGT 
     2221 ACTCCACACC CCCTAAGCGC CTCAAGACAA ATAGCTATGG TGGGAAGGAC CGAGGGGAAG 
     2281 ATGAGGAGAG CCGAGAACGG ATGGCTTCTG ATGTCACTAA CAACAAGGGC AATCTGGAAG 
     2341 ACCGCTGTCT GTCCTGCGGT AAGAAGAACC CTGTGTCCTT CCACCCCCTC TTTGAGGGTG 
     2401 GGCTCTGTCA GAGTTGCCGG GATCGCTTCC TGGAGCTCTT CTACATGTAC GATGAGGACG 
     2461 GCTATCAGTC CTACTGCACC GTGTGCTGTG AGGGCCGTGA ACTGCTTCTG TGCAGTAACA 
     2521 CAAGCTGCTG CAGGTGCTTC TGTGTGGAGT GTCTGGAGGT GCTGGTGGGT ACAGGCACAG 
     2581 CGGAGGATGC CAAGCTGCAG GAACCCTGGA GCTGCTATAT GTGCCTCCCG CAGCGCTGCC 
     2641 ATGGGGTCCT GCGGCGCAGG AAGGACTGGA ACATGCGCCT GCAAGACTTC TTCACTACTG 
     2701 ATCCTGACCT GGAAGAGTTT GAGCCGCCCA AGTTGTACCC AGCGATTCCT GCAGCCAAAA 
     2761 GGAGGCCCAT TAGGGTCCTG TCCCTGTTTG ATGGAATTGC AACAGGGTAC TTGGTGCTCA 
     2821 AGGACTTGGG TATTAAAGTG GAGAAGTACG TTGCCTCTGA AGTCTGTGCA GAGTCCATTG 
     2881 CTGTAGGAAC CATTAAGCAT GAAGGCCAAA TCAAATATGT CAATGACGTC CGGAAAATCA 
     2941 CCAAGAAAAA TATTGAAGAG TGGGGCCCAT TTGACTTGGT GATTGGCGGA AGCCCATGCA 
     3001 ATGATCTTTC TAATGTCAAT CCTGCCAGGA AAGGCCTGTA TGAGGGCACA GGAAGGCTCT 
     3061 TCTTTGAGTT TTACCACTTG CTGAATTATA CACGCCCCAA GGAGGGCGAC AACCGTCCAT 
     3121 TCTTCTGGAT GTTCGAGAAT GTTGTGGCCA TGAAAGTGAA TGACAAGAAA GACATCTCCC 
     3181 GATTCCTGGC ATGTAACCCA GTGATGATCG ACGCCATCAA GGTTTCTGCT GCTCACAGGG 
     3241 CCCGGTACTT CTGGGGTAAT CTACCTGGAA TGAACAGGCC TGTGATAGCT TCAAAGAATG 
     3301 ATAAGCTCGA GCTACAAGAC TGCTTGGAGT TCAGTAGGAC AGCAAAGTTA AAGAAAGTAC 
     3361 AGACAATAAC CACCAAGTCG AACTCCATCA GACAGGGGAA AAACCAGCTT TTCCCTGTAG 
     3421 TCATGAATGG CAAGGATGAC GTTCTGTGGT GCACTGAGCT CGAAAGGATC TTCGGGTTCC 
     3481 CAGCTCACTA CACAGACGTG TCCAACATGG GCCGGGGCGC CCGCCAGAAG CTACTGGGAA 
     3541 GGTCCTGGAG TGTGCCAGTC ATCAGACACC TGTTTGCCCC CTTGAAGGAC TACTTTGCAT 
     3601 GTGAAAGTGT GCCAGTCATC AGACACCTGT TTGCCCCCTT GAAGGACTAC TTTGCATGTG 
     3661 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3721 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3781 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3841 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3901 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3961 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4021 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4081 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4141 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4201 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4261 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4321 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4381 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4441 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4501 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4561 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4621 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4681 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4741 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4801 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4861 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4921 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4981 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5041 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5101 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5161 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5221 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5281 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5341 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5401 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5461 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5521 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5581 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5641 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5701 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5761 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5821 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5881 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5941 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6001 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6061 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6121 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6181 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6241 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6301 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6361 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6421 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6481 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6541 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6601 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6661 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6721 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6781 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6841 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6901 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6961 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7021 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7081 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7141 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7201 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7261 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7321 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7381 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7441 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7501 TTTAACGCGA ATTTT
//