LOCUS       RR201940                5261 bp    DNA     circular  SYN 
DEFINITION  Ins1 (Myc-DDK-tagged ORF) - Rat insulin 1 (Ins1), (10 ug)
ACCESSION   NM_019129
VERSION     NM_019129.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    1418..1509 
                     /label="Myc-DDK"  
     misc_feature    1539..2124 
                     /label="PolyA signal"  
     misc_feature    1596..1615 
                     /label="Restriction Enzyme"  
     misc_feature    2400..3072 
                     /label="ColE1"  
     misc_feature    3502..4296 
                     /label="Kan/Neo"  
     misc_feature    4331..4633 
                     /label="SV40 ori"  
     misc_feature    4719..5196 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    1400..1406 
                     /label="Restriction Enzyme"  
     misc_feature    1020..1400 
                     /label="Ins1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTGTGG ATGCGCTTCC TGCCCCTGCT GGCCCTGCTC GTCCTCTGGG 
     1081 AGCCCAAGCC TGCCCAGGCT TTTGTCAAAC AGCACCTTTG TGGTCCTCAC CTGGTGGAGG 
     1141 CTCTGTACCT GGTGTGTGGG GAACGTGGTT TCTTCTACAC ACCCAAGTCC CGTCGTGAAG 
     1201 TGGAGGACCC GCAAGTGCCA CAACTGGAGC TGGGTGGAGG CCCGGAGGCC GGGGATCTTC 
     1261 AGACCTTGGC ACTGGAGGTT GCCCGGCAGA AGCGTGGCAT TGTGGATCAG TGCTGCACCA 
     1321 GCATCTGCTC CCTCTACCAA CTGGAGAACT ACTGCAACAG TGCTGCACCA GCATCTGCTC 
     1381 CCTCTACCAA CTGGAGAACT ACTGCAACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT 
     1441 CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT 
     1501 TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG 
     1561 ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG 
     1621 TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG 
     1681 GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG 
     1741 GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG 
     1801 CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT 
     1861 GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG 
     1921 CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC 
     1981 TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT 
     2041 ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC 
     2101 ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG 
     2161 TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 
     2221 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 
     2281 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 
     2341 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 
     2401 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 
     2461 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 
     2521 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 
     2581 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 
     2641 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 
     2701 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 
     2761 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 
     2821 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 
     2881 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 
     2941 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 
     3001 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 
     3061 CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC 
     3121 GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT 
     3181 CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC 
     3241 GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC 
     3301 GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC 
     3361 ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC 
     3421 AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT 
     3481 TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA 
     3541 AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC 
     3601 ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT 
     3661 CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA 
     3721 TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG 
     3781 CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT 
     3841 CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT 
     3901 GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA 
     3961 TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT 
     4021 CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG 
     4081 GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG 
     4141 CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA 
     4201 CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA 
     4261 CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC 
     4321 ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC 
     4381 TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT 
     4441 CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT 
     4501 AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT 
     4561 GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA 
     4621 CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC 
     4681 TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     4741 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     4801 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     4861 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     4921 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     4981 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     5041 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     5101 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     5161 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     5221 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//