LOCUS       RR201858                6749 bp    DNA     circular  SYN 
DEFINITION  Rasgrp3 (Myc-DDK-tagged ORF) - Rat RAS guanyl releasing protein 3 (calcium and DAG-regulated) (Rasgrp3), (10 ug)
ACCESSION   NM_001108009
VERSION     NM_001108009.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2906..2997 
                     /label="Myc-DDK"  
     misc_feature    3027..3612 
                     /label="PolyA signal"  
     misc_feature    3084..3103 
                     /label="Restriction Enzyme"  
     misc_feature    3888..4560 
                     /label="ColE1"  
     misc_feature    4990..5784 
                     /label="Kan/Neo"  
     misc_feature    5819..6121 
                     /label="SV40 ori"  
     misc_feature    6207..6684 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2888..2894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2888 
                     /label="Rasgrp3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTCCAAC GGCCTCGGGA AAGCGGCGAC ACTAGATGAA CTTCTCAGCA 
     1081 CATGCATTGA GATGTTTGAT GACAATGGAG AACTGAATGA CAGCTACTTG CCCAGAATCA 
     1141 TGCTACTGAT GCATCGATGG TATTTATCCT CCACTGAACT GGCAGGAAAA CTCCTATGCA 
     1201 TGTATCGGAA TGCCAGCGGA GAAAGTTGTG ATGAGTTCCG ACTGAAGATC TGCTATTTCA 
     1261 TGAGGTACTG GATTACAAAA TTTCCTGCAG AGTTTAATTT GGATCTTGGT TTGATTCGCA 
     1321 TGACTGAGGA GTTCCGGGAA GTAGCTAGTC AACTAGGATA TGAGAAACAC AGCAACCTCA 
     1381 TGGACATATC CAGCATTCCT TCCTATGACT GGATGAGGAG AGTCACGCAG AGGAAAAAAG 
     1441 TATCAAAAAG GGGAAAGGCC TGTCTCTTAT TTGACCATCT GGAGCCCATT GAATTGGCTG 
     1501 AGCACCTCAC TTTTCTGGAG CATAAATCTT TTAGAAGGAT CTCGTTCACA GATTATCAGA 
     1561 GCTACGTCAT TCATGGCTGC CTGGAGAATA ACCCGACATT GGAGAGATCT ATTGCCTTAT 
     1621 TTAATGGAAT CTCTAAGTGG GTCCAATTGA TGGTTCTTAG CAAACCAAGT GCTCAACAAA 
     1681 GGGCGGAAGT CATCACAAAG TTCATCAATG TAGCACAGAA ACTCCTTCAA CTAAAAAATT 
     1741 TTAACACCTT GATGGCTGTG GTTGGTGGCC TTAGTCACAG CTCCATTTCC CGACTCAAAG 
     1801 ATACCCATTC TCACCTCTCT TCTGAAGTTA CAAAGAACTG GAATGAGATG ACAGAGTTGG 
     1861 TCTCCTCCAA CGGCAATTAC TGCAATTACC GCAAGGCCTT TGCTGACTGT GATGGCTTCA 
     1921 AAATCCCCAT CCTTGGCGTG CACTTGAAAG ACTTGATAGC AGTCCATGTC ATTTTCCCAG 
     1981 ACTGGATGGA AGAGAACAAA GTGAACGTTG TGAAAATGCA CCAGCTTTCT GTTACCCTGA 
     2041 GTGAACTGGT CTCCCTGCAG AATGCCTCTC ACCACTTGGA GCCTAACATG GATTTGATCA 
     2101 ACCTCCTCAC TCTCTCTCTG GACCTCTACC ACACAGAAGA CGATATTTAT AAACTATCAC 
     2161 TGGTGCTGGA GCCTAGGAAT TCCAAATCAC AGCCAACTTC CCCCACAACA CCTAATAAGC 
     2221 CTGTGGTACC CCTGGAGTGG GCTTCAGAAG TGGTACCAAA GCCAGACCCC ACAATCATCA 
     2281 ACAAACACAT AAGGAAGTTG GTTGAGCTCT GGGGCATAAT CAAACAAGGC TACAAATGCA 
     2341 AAGACTGTGG AGCCAACTGT CACAAACAGT GCAAAGACCT CTTGGTTCTG GCCTGTAGGA 
     2401 GACTTGCCAG GGCACCCTCC TTGAGCAGCA ACCCTGAGTC ACTGCCTGGA AGCCCAGCCT 
     2461 TGCCCCCAGT GCAGGATGAG GTGTTTGAGT TTCCTGGCGT CACTGCTAGA CACCGAGACC 
     2521 TGGACAGCAG AGACATCACG CTGGTTACAG GATCTTCCCG TAAGATCTCC GTGAGAGTGC 
     2581 AGAGAGCCAC CACTAGTCAA GCCACGCAAA CCGAACCTGT GTGGTCAGAG GCTGTCTGGG 
     2641 GAGACTCTGG GTCCCATACC TTCCCCAAAA TGAAATCCAA GCTCCATGAC AAAGCAGCAA 
     2701 AGGACAAAGG CTTTGCCAAG TGGGAAAATG AAAAGCCCAC GCTACACGCT GGTGTGGATG 
     2761 TTGTAGATCG AGGCACTGCA TTCGAACCTG ACCAGGACGA TGAGCAAGAT GAAGCCCAAC 
     2821 AGGGTGTCGA GGATGGCCAT TCGAACCTGA CCAGGACGAT GAGCAAGATG AAGCCCAACA 
     2881 GGGTGTCGAG GATGGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     2941 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     3001 CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG 
     3061 TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT 
     3121 TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG 
     3181 TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG 
     3241 AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC 
     3301 AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC 
     3361 TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT 
     3421 CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG 
     3481 CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG 
     3541 ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT 
     3601 TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA 
     3661 CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 
     3721 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 
     3781 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 
     3841 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 
     3901 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 
     3961 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 
     4021 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 
     4081 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 
     4141 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 
     4201 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 
     4261 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 
     4321 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 
     4381 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 
     4441 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 
     4501 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG 
     4561 GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG 
     4621 TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA 
     4681 TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG 
     4741 TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC 
     4801 TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG 
     4861 CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG 
     4921 AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT 
     4981 TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT 
     5041 GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA 
     5101 GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA 
     5161 GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC 
     5221 AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG 
     5281 CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA 
     5341 GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG 
     5401 GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT 
     5461 TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA 
     5521 GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG 
     5581 TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT 
     5641 CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG 
     5701 AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG 
     5761 GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT 
     5821 GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC 
     5881 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC 
     5941 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT 
     6001 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 
     6061 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT 
     6121 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG 
     6181 CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     6241 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     6301 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     6361 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     6421 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     6481 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     6541 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     6601 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     6661 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     6721 TGATTTAACA AAAATTTAAC GCGAATTTT
//