LOCUS       RR201857                8571 bp    DNA     circular  SYN 
DEFINITION  Cand1 (Myc-DDK-tagged ORF) - Rat cullin-associated and neddylation-dissociated 1 (Cand1), (10 ug)
ACCESSION   NM_054004
VERSION     NM_054004.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4728..4819 
                     /label="Myc-DDK"  
     misc_feature    4849..5434 
                     /label="PolyA signal"  
     misc_feature    4906..4925 
                     /label="Restriction Enzyme"  
     misc_feature    5710..6382 
                     /label="ColE1"  
     misc_feature    6812..7606 
                     /label="Kan/Neo"  
     misc_feature    7641..7943 
                     /label="SV40 ori"  
     misc_feature    8029..8506 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4710..4716 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4710 
                     /label="Cand1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGCT TCGTACCACA TCTCCAACTT GCTGGAAAAA ATGACATCCA 
     1081 GCGACAAGGA CTTCAGGTTT ATGGCTACAA ATGATCTGAT GACAGAACTG CAGAAAGACT 
     1141 CCATCAAGCT GGATGATGAC AGCGAAAGGA AAGTCGTAAA GATGATTCTG AAGTTGCTGG 
     1201 AGGATAAAAA TGGCGAAGTG CAGAACTTAG CTGTGAAATG TCTTGGTCCT TTAGTGAGTA 
     1261 AAGTGAAAGA GTACCAAGTT GAGACGATTG TAGACACCCT CTGTACAAAC ATGCTTTCTG 
     1321 ACAAGGAGCA GCTTCGAGAT ATTTCCAGTA TTGGCCTTAA AACAGTAATT GGAGAACTCC 
     1381 CTCCAGCTTC CAGTGGCTCT GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGCC 
     1441 TTACCAGTGC AATAGCAAAG CAGGAAGATG TTTCTGTTCA GCTAGAAGCT TTAGATATTA 
     1501 TGGCTGATAT GTTGAGCAGG CAAGGAGGAC TGCTTGTTAA TTTCCATCCT TCAATTCTGA 
     1561 CCTGTCTACT TCCCCAGCTG ACTAGCCCTA GACTTGCAGT CAGGAAAAGA ACAATTATTG 
     1621 CTCTTGGCCA CCTAGTTATG AGCTGCGGAA ACATAGTTTT TGTAGACCTT ATTGAACATC 
     1681 TGTTGTCAGA GTTGTCCAAA AATGACTCCA TGTCAACAAC AAGGACCTAC ATACAGTGTA 
     1741 TTGCTGCTAT TAGTAGGCAA GCTGGCCATA GAATAGGTGA ATACCTTGAA AAGATAATTC 
     1801 CTTTGGTGGT AAAATTTTGT AATGTAGATG ATGATGAATT AAGAGAATAC TGCATTCAAG 
     1861 CTTTTGAATC ATTTGTGAGA AGATGCCCTA AGGAAGTTTA TCCTCATGTT TCTACCATTA 
     1921 TAAACATTTG TCTAAAATAC CTTACCTATG ATCCAAATTA CAACTATGAT GATGAAGATG 
     1981 AAGATGAGAA TGCTATGGAT GCTGATGGCG GAGATGACGA TGACCAAGGG AGTGACGATG 
     2041 AATACAGTGA TGACGATGAC ATGAGTTGGA AAGTGAGGCG TGCGGCTGCC AAGTGCCTGG 
     2101 ATGCCGTAGT TAGCACACGG CATGAGATGC TCCCGGAATT CTACAAGACC GTCTCTCCTG 
     2161 CACTGATATC CAGATTTAAA GAGCGCGAAG AGAATGTAAA GGCAGATGTT TTTCATGCAT 
     2221 ACCTTTCTCT TTTGAAGCAA ACTCGTCCAG TACAAAGTTG GCTGTGCGAC CCTGATGCAA 
     2281 TGGAGCAAGG AGAAACACCC TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG 
     2341 CCCTACATAA ACAGATGAAA GAAAAAAGTG TAAAGACCCG ACAGTGTTGT TTTAACATGT 
     2401 TAACTGAACT GGTAAATGTG TTGCCTGGGG CACTAACCCA ACACATTCCT GTACTTGTAC 
     2461 CAGGAATCAT TTTCTCACTC AATGATAAGT CCAGCTCATC GAATCTGAAG ATTGATGCAT 
     2521 TGTCCTGTCT GTATGTGATC CTCTGTAACC ACTCTCCGCA AGTTTTCCAC CCGCATGTTC 
     2581 AGGCTTTGGT CCCTCCAGTG GTGGCTTGTG TTGGTGACCC ATTTTACAAG ATCACATCAG 
     2641 AAGCCCTTCT TGTCACTCAG CAGCTTGTCA AAGTAATCCG TCCTCTGGAC CAACCCTCCT 
     2701 CCTTCGATGC AACGCCTTAC ATCAAAGATC TCTTCACTTG TACAATTAAG CGCTTAAAAG 
     2761 CAGCTGACAT TGATCAGGAA GTCAAGGAGA GGGCTATTTC CTGTATGGGA CAGATTATTT 
     2821 GCAATCTTGG AGACAATTTG GGCCCTGACT TATCAAATAC ACTTCAGATT TTCTTGGAGA 
     2881 GACTCAAGAA TGAAATCACC CGGCTAACGA CAGTGAAAGC ACTGACCCTG ATTGCTGGGT 
     2941 CACCTTTGAA GATAGATCTG AGGCCTGTGC TGGGAGAGGG AGTCCCTATC CTTGCTTCAT 
     3001 TTCTCAGGAA AAATCAGAGA GCTTTGAAAC TGGGGACCCT CTCTGCCCTA GATATTCTCA 
     3061 TTAAAAACTA TAGTGACAGT TTGACGGCCG CCATGATTGA TGCAGTTCTG GATGAGCTCC 
     3121 CTCCTCTTAT CAGCGAAAGT GATATGCACG TGTCCCAGAT GGCTATCAGC TTCCTTACTA 
     3181 CCCTGGCAAA AGTATATCCC TCCTCCCTTT CAAAGATAAG CGGATCTATT CTCAATGAAC 
     3241 TGATTGGACT TGTAAGGTCA CCTCTGCTGC AGGGAGGAGC TCTTAGTGCC ATGCTAGACT 
     3301 TCTTCCAAGC TTTGGTTGTC ACTGGAACAA ACAATCTAGG ATACATGGAT TTGCTGCGCA 
     3361 TGCTAACGGG TCCAGTTTAC TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTACTATT 
     3421 CCATTGCCAA ATGTGTAGCT GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCGGCTGTAG 
     3481 TAGGTCAGTT TATTCAAGAT GTCAAGAACT CCAGGTCTAC AGATTCCATT CGTCTCTTAG 
     3541 CGCTCCTTTC TCTAGGAGAA GTTGGACACC ATATTGACTT AAGTGGGCAG TTGGAGCTAA 
     3601 AATCTGTAAT ATTAGAGGCT TTCTCCTCTC CTAGTGAAGA AGTTAAATCA GCTGCATCCT 
     3661 ATGCGTTAGG CAGCATCAGT GTAGGCAACC TCCCTGAGTA TCTGCCATTT GTACTACAAG 
     3721 AAATAACCAG TCAGCCCAAA AGGCAGTACC TGCTGCTGCA TTCCCTGAAG GAAATCATTA 
     3781 GCTCTGCGTC AGTGGTCGGC CTTAAGCCGT ATGTCGAGAA CATCTGGGCC TTGCTGCTAA 
     3841 AGCACTGTGA GTGCGCGGAG GAGGGCACCA GAAACGTCGT CGCCGAGTGT CTAGGGAAGC 
     3901 TCACTCTTAT TGATCCTGAA ACTCTCCTCC CGAGGCTTAA AGGGTATTTG ATATCAGGGT 
     3961 CATCCTATGC CAGAAGCTCA GTGGTTACAG CTGTGAAGTT CACTATTTCT GACCACCCTC 
     4021 AGCCCATTGA TCCACTGCTC AAGAACTGCA TAGGTGATTT TCTAAAAACG TTGGAAGACC 
     4081 CAGATTTGAA TGTAAGAAGA GTGGCCTTGG TCACATTCAA TTCTGCAGCC CATAACAAGC 
     4141 CGTCACTGAT ACGGGACCTT CTGGATTCTG TTCTTCCACA TCTTTACAAT GAGACAAAAG 
     4201 TTAGGAAGGA ACTTATAAGA GAGGTAGAGA TGGGCCCGTT TAAGCACACG GTTGATGACG 
     4261 GCCTGGACAT TAGAAAGGCA GCTTTTGAGT GTATGTACAC ACTTCTAGAC AGCTGTCTTG 
     4321 ACAGACTGGA TATCTTTGAA TTTCTAAATC ATGTGGAAGA TGGTTTGAAG GACCATTATG 
     4381 ATATTAAGAT GCTAACATTT TTAATGTTGG TGAGACTATC TACCCTTTGT CCAAGTGCAG 
     4441 TACTACAGAG GTTGGACCGG CTTGTTGAGC CACTACGAGC TACGTGTACA ACTAAGGTGA 
     4501 AGGCGAACTC TGTAAAGCAG GAGTTTGAAA AGCAGGACGA GTTAAAGCGC TCGGCCATGA 
     4561 GGGCAGTAGC TGCACTGCTC ACCATTCCAG AGGCAGAGAA GAGCCCTCTC ATGAGTGAGT 
     4621 TCCAGTCACA GATCAGCTCC AACCCGGAGC TGGCAGCCAT CTTTGAAAGT ATCCAGAAAG 
     4681 ATTCTTCGTC CACTAACTTG GAATCAATGG ACACTAGTAC GCGTACGCGG CCGCTCGAGC 
     4741 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4801 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4861 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4921 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4981 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5041 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5101 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5161 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5221 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5281 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5341 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5401 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5461 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5521 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5581 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5641 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5701 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5761 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5821 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5881 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5941 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6001 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6061 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6121 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6181 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6241 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6301 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6361 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6421 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6481 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6541 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6601 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6661 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6721 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6781 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6841 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6901 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6961 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7021 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7081 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7141 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7201 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7261 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7321 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7381 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7441 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7501 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7561 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7621 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7681 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7741 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7801 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7861 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7981 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8161 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8221 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8281 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8341 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8401 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8461 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8521 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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