LOCUS       RR201779                9066 bp    DNA     circular  SYN 
DEFINITION  Tcof1 (Myc-DDK-tagged ORF) - Rat Treacher Collins Franceschetti syndrome 1, homolog (Tcof1), (10 ug)
ACCESSION   NM_001106143
VERSION     NM_001106143.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5223..5314 
                     /label="Myc-DDK"  
     misc_feature    5344..5929 
                     /label="PolyA signal"  
     misc_feature    5401..5420 
                     /label="Restriction Enzyme"  
     misc_feature    6205..6877 
                     /label="ColE1"  
     misc_feature    7307..8101 
                     /label="Kan/Neo"  
     misc_feature    8136..8438 
                     /label="SV40 ori"  
     misc_feature    8524..9001 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5205..5211 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5205 
                     /label="Tcof1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGCC AGGAAGCGGC GGGAGCTGCT TCCCCTTATT TACCATCATC 
     1081 TGTTGCAAGC AGGCTACGTC CGCGCGGCGC GGGAAGTTAA GGAGCAGAGC GGCCAGAAGA 
     1141 ATTTCCTGAC TCATCCCGTC ACCCTTCTGG ACATCTATAC ACACTGGCAA CAGACCTCAG 
     1201 AGCTCGGCCT GAAGCAGAAG GCAGAGAATG ATGAGACACT GCAAGCCAAG AAGTCTCGAG 
     1261 TGTCAGACCC TATTAGCAGC TCAGAGAGCT CAGAGGAGGA GGAAGAGGAG GCAGAAACGG 
     1321 GAACTGCCAA AGCCACCCCA AGACCGACAC CTGTCAATTC CACAGCTGCA GCTTTGCCAT 
     1381 CAAACATGAA ACAAAAGGCA AAGACCAAGA CAGCCAACAA GACGGTGAAC TCCGTGCTGC 
     1441 CCCCTGCGTC CGGAAAGACA GCGGTCCAGC TGCTGTCTGG GAAGTCACCC AAGAAGTCAG 
     1501 CAGAGCCCTT GGCCAGCATT GTCTTGGCCT CAGAAACTGA GGAGGAGGGC AGTGCCCAAG 
     1561 TCCTGGGACC CACTGCCAAG CCTGGAATGG TGTCAGCAGG CCAAGCCAGC AGTTCCAGCG 
     1621 AGGATACCTC CAGCTCAAGC GATGAGACAG ATGTGGAGGT GAAACCCTCA ACAAAACCAC 
     1681 AGGCCAAAGC TCCAGTAACT CCTGCCAAGG ATCCTCCAGC AAGAACAGCC TCAGGCCCTA 
     1741 CCAAGCTAGG GGATGTGACA CCCGCATCAG CAAGGGCTGC AGCCACTAAA GCGGCAGAGT 
     1801 CTGAGAGCAG TGAGGAGGAC TCAGAGAGTG AGGACGAGGC CCCTGCTGGC CTGCCTAGCC 
     1861 AGGTAACAAC CCCTGGAAAA GGTCTTCATG TCAGAGCTGC CTCAGTTTCT GCCAAGGGGA 
     1921 TGTCTAGGAA AGGGCCCATC TCAGCAACCC CAGAGAAGAC TGGGCCCATA ACCCCCCAAG 
     1981 CCAAGACAGG AAGGCCACAG AAGGATATGG AAACCAGCAG TGAGGACGAT TCTGACAGTG 
     2041 AGGATGAGAT GCCAGTCACT GTGACCACTC CTCAGGCAAG GCCTTCCGGG AAGAGCCCTC 
     2101 AGGTCAGAGG TACCTCTGCC CCTGCCAAGG AGTCATCCCA GAAAGGGGCT CCTGCAGTCA 
     2161 CCCCTGGGAA GACAAGACCT GTGGCAGCCC AGGCCCAGGC AGGGAAGCCA GAGACCAGGA 
     2221 GCAGTGAGGA GTCAGAGAGC GACAGTGGAG AGACACCAGC AGCTGGGACT CTAACTACAA 
     2281 GTCCTGCCAA GGTGAAACCT TTGGGGAAAA GCCCCCAGGT CAGACCTGCC TCCACTGCAA 
     2341 ACCTGGGGTC ATCTAGAAAA GGAGCCAACC CACCCTGCCT TGGGAAGGTG GGGTCAGCAG 
     2401 TTCTCAAGGT CCAAACGGGA AAGGAAGAAG AGGACTCAGA GAGCAGCAGC GAGGAGTCGG 
     2461 ACAGTGACGG GGCTGTGAAC GCAGCCAAGG CAAAGCCCCC AGGGAGAAAA GCCACTCCAG 
     2521 CACTCCCTCG GAAGACGGGG CCTGTGGCCA CCCAGGTCAA GACTGACAAG GGCAAAGACC 
     2581 ACGCAGAGAG CAGCGAGGAG TCGACCGACA GCGAGGAGGA GGCAGCACCA GCAGCTTCTG 
     2641 CTGCTCAGGC TAAACCAGCT CCGATAAAGC AGATGAAAGC TTCCCCTAGG AAAGGCACGG 
     2701 CTGCATCCAC CACAGGGGCG AGCGCCTCGT CCCCTCGTAA GGCAGGAACA AAGACCTCTT 
     2761 CAGCCAGCCT ATCATCCCTG GCTCTGCCCA AGGGCACCCA GAAGCCAGAT GTGGACTCCT 
     2821 CTAGCGAGTG GGAGTCAGAA GGAGCTGCTC CTGGCACCAC CGGGGTACAG GGAAAGTCTG 
     2881 GGGGAAAGGG CCTCCAAGGG AGAGCTGCCT CAGGACAAGG GGTGGCCCCA CTGCACGCTC 
     2941 AGAAGACAGG GCCTTCAGGT GCCCAGGTCA AAGCCACAGC ACAGGAAGAC TCAGAGAGCA 
     3001 GCGAAGAGGA ATCCAGCAGT GAAGAAGAGG ATGAGACCCC AGCACAGGTC ATGGCCTTGG 
     3061 GGAGACTTCC TCCGGCCAAA GCCAACCCAC CTCCCACTAA GACACCTCTG GCATCTGCAT 
     3121 CTGGAAAAGC GGCCGCAGTA GTTCCTCCAC CCAAGGGAAA AGCACCTGCG AGTACTGTTC 
     3181 AGAACAGCAC CATCTCTGCC AGGGGCCAGC GGGCTGTGCC GGCCACGGGA AAAGCAGGGG 
     3241 CCCCAGCAAC CCAAGCACAG AAGGGGCCCA TGGCTGGCAC AGGGGAGGAC TCAGAGAGCA 
     3301 GCAGTGAAGA GGAGTCTGAC AGTGAAGAAG AAACTCCAGC CCAGGTCAAG CCTGTGGGGA 
     3361 AGACCTCTCA GGTCAGAGCT GCCTCGGCCC CTGTCAAGGA GTCTCCTAAC AAAGGAGCTT 
     3421 ATTCAGGAAC CTCCAGGAAG ACAGGGCCTC CAGCTACCCA AGCTCAGACA GGGAAGACAG 
     3481 AGGACTCAGA GAGCAGTAGT GAGGAATCTG ACAGTGACAG AGAGATACCA CCAGCCATCA 
     3541 CCCCAGCCCA GGATGGTATC TCCCAGTCTA CCAGAAGCAA ACTCTCAGGT CTGGCACCCC 
     3601 CAGAGAAGAG CACAGAAGAG TCCTCAGAGA GCAGTGATGA GGATCTGCCA TCTGGCCAGG 
     3661 CCATTAAATC CCCTCCAATT TCTGTCAACC CTAATCGTGG TCCAGCTGCC CCAGTTCCAA 
     3721 CCCCAGAACA ACACCAGGCT GTGAACACAA GGAAGGCCCA GGCCTCAGGC AGCACTGCCC 
     3781 AGAGCTCCTC CTCCGAGAGT GAGGATGAGG ACATGATTCC TGCCACACAA CCCCCCACCC 
     3841 TTGCCATCAG AACCAATGTG ACTACGCCCA CAGCCCTCTC ACAAACAGCC GCCCAACCCA 
     3901 GCAAGAGTGA GCAGTCTAGT CGGATGCCAA AAGGCAAGAA ACCAAAGACA GCGTCCACTC 
     3961 AGATCAGCAG TGCCATGGAA GCACTCCCCG TGACGCTTCC CCAGAGCACA CCTGCCCAGT 
     4021 CCAAAACCAC CAACAAGCTG GGGGACCCCA AACTTGCTGA GAAGCAGCAG CTTACCCCAG 
     4081 GCTACCCCAA AGCCCCCAGG AGCTCAGAGG ACAGCAGTGA CACCTCTTCA GAGAGCGAGG 
     4141 AGGATGCCAA GAGACCCCAG ATGTCCAAGT CGTCCCAAAG GCTGGATCCA GACGCTTCCC 
     4201 AGAAGGAAAC TGTGGTAGAG GAGACCCCTA CAGAATCCAG CGACGACGAG ATGGTGGCCC 
     4261 CCTCACAGTC TCTCCTCTCA GGTTATGTGA CTCCCGGCTT AACTGTGGCC AATTCCCAGC 
     4321 CTTCAAAAGC TACTCCTAGG CCAGACGCCA ACCCCTTGGT TTCCTCTGCT CCAGCCACCA 
     4381 AAGATAACCC AGATGGCAAG CAGAAATCAA AATCCCAAGA CTCCACTGCA GACACCACAC 
     4441 TCCGTAAAAC CGGTAGGAAA GAGGCCTCCT CAGGATCCAC ACCTCAGAAG CCCAAGAAGC 
     4501 CCAAGAAGAG CACCTTGAGC TCACCAGCCC CGGCACAGAC ACTGCCGAAC AGCATCACCC 
     4561 AGCGCCTCCT GGAGCAGCCC TGGCCCCTGA GTGAGGCACA GGTGCAGGCC TCTGTGATGA 
     4621 AGGTCCTGAC AGAACTGCTG GAGCAGGAAA GGCAGAAGGC CACCGAGGCC ATCAAGGAGA 
     4681 GTGGAAAGAA GGGCCAGAAA CGGAAACTAT CAGGGGACCA GGTAGAAGCT GGGGCCCCAA 
     4741 AGAACAAGAA GAAGAAGCAG CAGCTTGCGG CTGGGACAAG TGCTGGTTCC CCAGAAAAGG 
     4801 CCTCCAGGAC TTCCAAGGCA AAATCAAAAC TAAACAAAGG GAGTGCTGGT GGCAAGGGGA 
     4861 AGGGGTCTCC TGTCCCCCAA GGAGCCAAGG AGAAGCCCGA AGGCAAGCTG GGGATAAAGC 
     4921 TTGAGAGTGG AGAGCAGAGT GACCCGAAGA GCAAGAAGGA AAAGAAGAAA TCCAGTAAGA 
     4981 AAAGAAAAAA GGAAAAAAGA CCCCAGCCAA GGACTCTGCC TCACCGGTCC AGAAGAAGAA 
     5041 AAAGAAGAAG AAGAAGACAG CCGAGCCTGC TGTGTGAGGG CCAGGCAGCG CATAGAACAG 
     5101 AGTCTTGGCA GAGCGTGACC ATCCCCAGGC CTCTGACCTC TGGGCACTGT GAGTTGGGAC 
     5161 TGGACTTTTT AATTTGCCCT CCCTCGGCAG AAGGTGATGG CTGGCGTTGC TGCACGCGTA 
     5221 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5281 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5341 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5821 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5881 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5941 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6001 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6061 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6841 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6901 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6961 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7021 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7081 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7141 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7201 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7261 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7321 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7381 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7441 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7501 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7561 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7621 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7681 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7741 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7801 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7861 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7921 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7981 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8041 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8101 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8161 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8221 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8281 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8401 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8461 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8521 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8581 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8641 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8701 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8761 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8821 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8881 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8941 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9001 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9061 AATTTT
//