LOCUS       RR201766                8391 bp    DNA     circular  SYN 
DEFINITION  Thbs1 (Myc-DDK-tagged ORF) - Rat thrombospondin 1 (Thbs1), (10 ug)
ACCESSION   NM_001013062
VERSION     NM_001013062.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4548..4639 
                     /label="Myc-DDK"  
     misc_feature    4669..5254 
                     /label="PolyA signal"  
     misc_feature    4726..4745 
                     /label="Restriction Enzyme"  
     misc_feature    5530..6202 
                     /label="ColE1"  
     misc_feature    6632..7426 
                     /label="Kan/Neo"  
     misc_feature    7461..7763 
                     /label="SV40 ori"  
     misc_feature    7849..8326 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4530..4536 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4530 
                     /label="Thbs1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCCTC AGGGGACTAG GCGTCCTGTT CCTGTTGCAT GTGTGTGGAA 
     1081 GCAACCGCAT TCCAGAGTCT GGGGGAGACA ATGGTGTGTT CGACATTTTT GAACTCATTG 
     1141 GAGGTGCCCG CAAGGTTCCG GGTCGCCGAC TGGTGAAGGG CCAAGATCTA TCCAGCCCCG 
     1201 CCTTCCGGAT CGAGAATGCC AACCTGATCC CCCCTGTGCC AGATGACAAG TTCCAAGACC 
     1261 TACTGGACGC TGTGTGGGCC GACAAAGGCT TCATCTTCCT GGCTTCCTTG AGGCAGATGA 
     1321 AGAAGACTCG GGGCACACTC CTGGCCGTGG AACGGAAAGA CAATTCTGGC CAGATCTTCA 
     1381 GTGTGGTCTC CAATGGCAAA GCCGGCACCC TCGATCTGAG TCTGAGCCTG CCCGGGAAGC 
     1441 AGCAAGTGGT GTCAGTGGAG GAAGCTCTCC TGGCCACTGG CCAGTGGAAG AGCATCACGC 
     1501 TGTTTGTCCA AGAAGACAGG GCCCAGCTCT ACATTGACTG TGACAAGATG GAGAGCGCAG 
     1561 AGCTGGATGT TCCCATCCAG AGCATCTTCA CAAGGGATTT GGCCAATGTC GCCAGGCTCC 
     1621 GAGTCGCAAA GGGAGATGTC AATGACAATT TTCAGGGGGT GCTGCAGAAT GTGAGGTTTG 
     1681 TCTTTGGAAC CACCCCAGAA GACATTCTCA GGAACAAAGG CTGCTCCAGT TCAACCAACG 
     1741 TCCTTCTCAC CCTTGACAAC AACGTGGTGA ACGGTTCCAG CCCTGCCATC CGCACCAACT 
     1801 ACATCGGCCA CAAAACAAAG GACCTCCAAG CCATCTGTGG CCTCTCCTGT GACGAACTAT 
     1861 CCAGCATGGT CCTGGAACTG AGGGGCCTGC GTACCATCGT GACCACGCTA CAGGACAGCA 
     1921 TTCGCAAAGT GACGGAAGAG AACAGAGAGC TGGCTAGCGA GCTGAGGCGG CCTCCCCTCT 
     1981 GCTTCCACAA TGGAGTCCAA TACAGGAACA ACGAGGAGTG GACTGTAGAT AGTTGCACAG 
     2041 AGTGTCACTG CCAGAACTCA GTTACCATCT GCAAAAAGGT GTCCTGTCCC ATCATGCCCT 
     2101 GCTCCAATGC CACAGTTCCT GATGGTGAAT GCTGCCCACG GTGCTGGCCC AGTGACTCTG 
     2161 CTGACGATGG CTGGTCTCCC TGGTCTGAGT GGACCTCTTG CTCTGCCACC TGTGGCAATG 
     2221 GGATTCAGCA ACGTGGTCGC TCCTGTGACA GCCTCAACAA CAGATGTGAG GGCTCTTCAG 
     2281 TACAGACGAG GACCTGCCAC ATTCAGGAGT GCGACAAAAG ATTTAAACAG GATGGTGGTT 
     2341 GGAGTCACTG GTCCCCGTGG TCGTCCTGTT CTGTGACCTG TGGTGACGGT GTGATCACGA 
     2401 GGATCCGACT CTGCAACTCC CCCAGCCCCC AGATGAACGG GAAGCCCTGT GAAGGTGAAG 
     2461 CTCGGGAGAC CAAAGCCTGC AAGAAGGACG CCTGCCCAAT CAATGGAGGC TGGAGTCCCT 
     2521 GGTCACTATG GGACATCTGC TCTGTCACCT GTGGAGGAGG AGTACAGAGA CGTAGCCGAC 
     2581 TCTGCAACAA CCCCACACCC CAGTTTGGAG GCAAAGACTG TGTTGGTGAT GTGACGGAAA 
     2641 ATCAAGTTTG CAACAAGCAG GACTGTCCGA TTGATGGATG CCTGTCCAAT CCCTGCTTTG 
     2701 CTGGTGCCAA GTGTACGAGC TATCCTGATG GTAGCTGGAA ATGTGGTGCG TGTCCTCCTG 
     2761 GCTACAGTGG AAATGGTATC CAGTGCAAAG ACGTCGACGA GTGCAAAGAA GTGCCTGATG 
     2821 CTTGCTTCAA TCACAACGGG GAACATCGGT GCAAGAACAC AGATCCTGGC TACAACTGCC 
     2881 TGCCCTGCCC ACCACGATTC ACCGGCTCAC AGCCCTTCGG CAGAGGTGTC GAACACGCTA 
     2941 TGGCCAACAA ACAGGTGTGC AAACCCCGAA ACCCCTGCAC CGACGGGACA CACGACTGCA 
     3001 ACAAGAACGC CAAGTGCAAC TACCTGGGTC ACTACAGCGA CCCCATGTAC CGCTGCGAGT 
     3061 GCAAGCCTGG CTATGCAGGC AATGGCATCA TTTGCGGAGA GGACACAGAC CTTGACGGCT 
     3121 GGCCTAATGA AAACCTGGTG TGTGTGGCCA ACGCAACCTA CCACTGCAAA AAGGACAACT 
     3181 GCCCCAATCT TCCCAATTCG GGGCAGGAAG ACTATGACAA GGATGGGATT GGCGATGCCT 
     3241 GTGATGACGA TGATGACAAC GACAAGATTC CTGATGACAG GGACAACTGT CCATTCCATT 
     3301 ACAACCCTGC CCAGTATGAC TATGACAGAG ATGATGTGGG AGACCGCTGT GACAATTGCC 
     3361 CCTACAACCA CAACCCTGAC CAGGCAGACA CAGACAACAA TGGGGAGGGA GATGCCTGCG 
     3421 CTGTGGACAT CGATGGGGAT GGAATCCTCA ATGAACGAGA CAACTGCCAG TATGTTTACA 
     3481 ACGTGGATCA GAGGGACACG GACATGGATG GGGTTGGAGA CCAGTGTGAC AACTGCCCCC 
     3541 TGGAACACAA TCCAGACCAG CTGGACTCTG ACTCGGACCG CATAGGGGAC ACCTGTGACA 
     3601 ACAATCAGGC CATCGATGAG GATGGCCATC AGAACAACCT TGAAAACTGT CCCTATGTGC 
     3661 CCAATGCCAA CCAGGCCGAC CACGATAAAG ATGGTAAAGG AGACGCCTGT GACCATGACG 
     3721 ATGACAACGA CGGCATCCCT GATGACAGAG ACAACTGCAG GCTGGTGCCC AATCCTGACC 
     3781 AGAAGGACTC TGATGGTGAT GGCCGAGGCG ATGCCTGCAA AGACGACTTT GACCATGACA 
     3841 ATGTGCCAGA CATTGATGAC ATCTGTCCTG AGAATGTTGA CATCAGTGAA ACCGATTTCC 
     3901 GCCGATTCCA GATGATTCCT CTAGATCCCA AAGGAACCTC CCAAAATGAC CCTAACTGGG 
     3961 TTGTCCGCCA TCAGGGCAAA GAACTTGTCC AGACTGTAAA CTGTGACCCT GGACTTGCTG 
     4021 TAGGTTATGA TGAGTTTAAT GCCGTGGACT TCAGTGGTAC CTTCTTCATC AACACTGAGA 
     4081 GGGATGACGA CTATGCTGGC TTTGTTTTCG GGTACCAGTC CAGCAGCCGC TTCTACGTTG 
     4141 TGATGTGGAA ACAAGTCACC CAGTCCTACT GGGACACCAA CCCCACAAGG GCTCAGGGAT 
     4201 ACTCAGGCCT GTCTGTAAAG GTTGTAAACT CCACCACTGG CCCCGGCGAG CACCTGCGGA 
     4261 ATGCACTGTG GCACACAGGA AACACCCCTG GCCAGGTGCG CACCCTGTGG CATGACCCTC 
     4321 GTCACATTGG CTGGAAAGAT TTCACTGCAT ACAGATGGCG TCTCAGCCAC AGGCCAAAGA 
     4381 CCGGTTTGAT CAGAGTGGTG ATGTATGAAG GAAAGAAAAT CATGGCTGAC TCAGGACCCA 
     4441 TCTATGACAA AACCTACGCT GGCGGTAGAC TAGGCCTGTT CGTCTTCTCT CAAGAAATGG 
     4501 TGTTCTTCTC GGACATGAAA TACGAGTGCC GAGACTCCAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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