LOCUS       RR201755                9432 bp    DNA     circular  SYN 
DEFINITION  Duox2 (Myc-DDK-tagged ORF) - Rat dual oxidase 2 (Duox2), (10 ug)
ACCESSION   NM_024141
VERSION     NM_024141.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5589..5680 
                     /label="Myc-DDK"  
     misc_feature    5710..6295 
                     /label="PolyA signal"  
     misc_feature    5767..5786 
                     /label="Restriction Enzyme"  
     misc_feature    6571..7243 
                     /label="ColE1"  
     misc_feature    7673..8467 
                     /label="Kan/Neo"  
     misc_feature    8502..8804 
                     /label="SV40 ori"  
     misc_feature    8890..9367 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5571..5577 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5571 
                     /label="Duox2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTCCAACA AGTCTCAAGA CATTGGTGCT CCTGGGCGCT CTGTTGACTG 
     1081 GACCCCTGGG CCCAGCAGGT GGCCAGGATG CACCCTCACT GCCCCGGGAA GTGCAGCGCT 
     1141 ACGACGGCTG GTTTAATAAT CTCAAGTACC ACCAGCGCGG TGCAGCTGGC TCGCAGCTGC 
     1201 GTCGCCTAGT ACCTGCTAAT TACGCTGACG GTGTTTATCA GGCTCTGCAG GAGCCGCTAC 
     1261 TCCCCAACGC TCGCCTGCTA AGCGACGCTG TCAGTAAGGG CAAAGCAGGG CTGCCCTCGG 
     1321 CGCACAACCG CACAGTGCTT GGGCTCTTCT TTGGCTACCA CGTGCTCTCA GACCTGGTGA 
     1381 GTGTGGAAAC ACCAGGCTGC CCCGCAGAGT TCCTCAACAT TTACATCCCA CGTGGGGACC 
     1441 CAGTGTTTGA CCCTGACAAG CGCGGGAACG TGGTGCTGCC CTTTCAGAGG AGCCGCTGGG 
     1501 ACCGCAGCAC TGGACAGAGC CCTAGCAACC CCCGAGACCT GACCAACCAG GTGACTGGCT 
     1561 GGTTAGATGG CAGCGCCATC TATGGCTCCT CTCATTCCTG GAGCGACACT CTGAGGAGCT 
     1621 TCTCTGGAGG ACAGCTGGCT TCTGGCCCTG ACCCTGCTTT CCCGAGGAAC TCCCAGAACT 
     1681 CTCTGCTCAT GTGGATGGCA CCAGACCCCG CCACGGGGCA GGGCGGGCCA CAGGGGCTGT 
     1741 ATGCCTTCGG GGCCCAGCGC GGGAACAGGG AGCCCTTTCT GCAGGCACTG GGCTTGTTGT 
     1801 GGTTTCGATA CCACAACCTG TGTGCCAAGA GGCTGGCGCA GGAGCACCCA CACTGGGGGG 
     1861 ATGAGGAGCT GTTCCAGCAT GCTCGCAAGA GGGTCATTGC CACTTACCAG AACATTGCTC 
     1921 TATACCAATG GTTGCCCAGC TTCCTGCAGA AAACACCCCC AGAGTATTCC GGGTACCGCC 
     1981 CTTTCATGGA CCCCAGCATC TCCCCAGAGT TCGTGGCGGC CTCAGAGCAG TTCCTCTCTA 
     2041 CCATGGTGCC CCCGGGGGTC TATATGAGAA ACTCCAGCTG TCACTTCCGG GAATTCCCAA 
     2101 AGGAAGGTTC CAGCAGCTCT CCAGCTCTCA GGGTCTGCAA CAACTACTGG ATTCGGGAGA 
     2161 ACCCCAGTCT GAAGACTGCT CAGGACGTGG ACCAGCTGCT GCTGGGAATG GCTTCCCAGA 
     2221 TTTCAGAGCT GGAGGACAGG ATAGTGATTG AAGATCTGAG AGATTACTGG CCTGGCCCAG 
     2281 ACAGATACTC TCGCACAGAC TACGTGGCTA GCAGTATCCA GAGTGGCCGA GATATGGGGC 
     2341 TGCCCAGTTA TAGCCAGGCT CTGCAGGCCT TAGGACTGGA GCCTCCCAAG AATTGGAGTG 
     2401 CTCTCAACCC CAAAGTGGAT CCCCAGGTGC TGGAGGCCAC AGCTGCTCTG TACAACCAGG 
     2461 ACCTGTCCCG GTTGGAACTA TTCCTGGGAG GGCTCCTGGA GAGCCATGGG GACCCTGGAC 
     2521 CTCTATTCAG CAACATCATT CTTGACCAGT TTGTAAGGCT CCGGGATGGT GATCGCTACT 
     2581 GGTTTGAGAA CACTAGGAAC GGGCTGTTCT CCAAGGAGGA GATTGCAGAA ATCAGAAACA 
     2641 CCACCTTGCG GGATGTACTG GTAGCTGTCT CCAATGTGGA CCCCAGTGCC TTGCAGCCCA 
     2701 ATGTTTTCTT CTGGCAAGAA GGTGCACCCT GCCCACAGCC TCAGCAACTC ACAACTGAAG 
     2761 GCTTGCCCCA GTGTGTGCCT GTTACTGTGA TTGACTACTT TGAGGGCAGT GGTGCTGGCT 
     2821 ATGGTGTCAC CCTCCTGGCT GTCTGCTGCT TTCCAGTAGT GAGTCTGATT ATTGCTTGGG 
     2881 TGGTGGCTCG TTTCCGGAAC CGAGAACGCA AGATGCTACT AAAGAAAGGC AAGGAGAGTC 
     2941 TAAAGAAACA GACAGCCAGT GATGGAGTAC CAGCGATGGA GTGGCCAGGC CCCAAGGAGA 
     3001 GCAGCTACCC AGTCACTGTC CAGCTGCTTC CAGACAGGAG TCTGAAGGTC CTTGACAAAA 
     3061 GGCTCACTGT GCTCCGTACC ATCCAACTGC AGCCCACACA GCAGGTCAAC CTCATCCTGT 
     3121 CCAGCAGCCA TGGACGCCGC ACCCTGTTGC TCAAGATCCC CAAGGAGTAT GACCTGGTGC 
     3181 TGATGTTTAA CTCTGAAGAG GACCGGGATG CCTTCGTGCA GCTGTTGCAA GACCTCTGCG 
     3241 TCTGCTCCAC TCCCGGCCTC CGCATAGCTG AGATGGATGA GAAGGAGCTA CTGAGAAAGG 
     3301 CTGTGACCAA GCAGCAGCGG GCAGGCATCT TGGAGATCTT CTTCAGACAG CTTTTTGCTC 
     3361 AGGTGCTGGA CATCAATCAG GCCGACGCAG GGACTCTGCC TCTGGACTCA TCCCAGCAAG 
     3421 TACGAGAGGC TCTGACCTGT GAACTGAGCA GAGCGGAGTT TGCGGACTCC CTGGGCCTCA 
     3481 AGCCCCAGGA TATGTTTGTG GAGTCCATGT TTTCTCTGGC CGACAAGGAT GGCAATGGCT 
     3541 ACATATCCTT CCGGGAGTTC CTGGACATCC TGGTGGTTTT CATGAAAGGT TCCCCACAGG 
     3601 ACAAGTCTCG CCTAATGTTT ACCATGTATG ACCTGGATGG AAATGGCTTT CTCTCCAAGG 
     3661 AGGAATTCTT CACCATGATG CGGTCCTTCA TCGAGATCTC CAACAACTGC CTGTCCAAGG 
     3721 ACCAGCTGGC TGAGGTGGTG GAATCCATGT TCCGGGAGTC GGGCTTCCAG GACAAGGAGG 
     3781 AGCTGACCTG GGAGGACTTC CATTTCATGC TGCGCGACCA CGACAGCGAT CTCCGATTCA 
     3841 CACAGCTCTG TGTCAAAGGT GGGGCTGGAG GCACCGGGGA CATCTTTAAG CAAAGCAATG 
     3901 CCTGTCGAGT CTCATTCCTC ACCCGGACTC CTGGGAACAG GGTAATGGCT CCCTCTCCCC 
     3961 GGCTGTACAC GGAAGCACTG CAGGAGAAAA TGCAGAGGGG CTTCCTAGCC CAGAAGCTCA 
     4021 AGCAGTTCAA GCGGTTTGTG GAAAACTACC GGCGCCACAT CGTGTGTGTT ACAATCTTCT 
     4081 CAGCCATCTG CGCAGGCCTG TTTGCAGACC GTGCCTACTA CTATGGCTTT GCCTCGCCAC 
     4141 CCACGGACAT TGAAGAAACC ACCTATGTGG GCATCATCCT GTCCCGGGGC ACAGCAGCCA 
     4201 GCATCTCCTT CATGTTCTCC TACATCCTGC TCACCATGTG CCGCAACCTC ATCACCTTCC 
     4261 TGCGGGAGAC CTTCCTCAAC CGCTACATTC CCTTTGATGC CGCTGTGGAC TTCCACCGCT 
     4321 GGATCGCCAT GGCTGCGGTT GTCCTAGCTG TTGTGCACAG TCTTGGACAT GCAGTCAACG 
     4381 TGTACATCTT CTCAGTCAGT CCCCTCAGCC TGATGACCTG CGTCTTCCCT AGTGTCTTTG 
     4441 TGAATGATGG GTCCAAGCTT CCCCCAAAGT ACTACTGGTG GTTCTTCGAG ACAGTTCCAG 
     4501 GTATGACAGG AGTACTCCTG CTCCTGGTCC TGGCTATCAT GTATGTGTTT GCCTCCCACC 
     4561 ACTTCCGCCG CCACAGTTTC CGGGGCTTCT GGTTGACCCA CCACCTCTAT GTTGTGCTTT 
     4621 ATGCCCTGAT CATCATCCAT GGCAGCTATG CCCTCATCCA ATTACCCAGC TTCCACATCT 
     4681 ACTTCCTGGT CCCCGCCATT ATCTATGTGG GGGACAAGCT GGTGAGCCTG AGCCGGAAGA 
     4741 AGGTGGAGAT CAGTGTGGTG AAGGTTGAGC TGCTGCCTTC AGGGGTGACC TATCTGCAGT 
     4801 TCCAGCGGCC CAAGACTTTT GAGTACAAAT CCGGGCAGTG GGTGCGGATC GCTTGCTTGA 
     4861 GTCTGGGAAC CAATGAGTAC CACCCCTTCA CGCTGACCTC CGCACCCCAT GAGGACACAC 
     4921 TCAGCCTGCA CATCCGGGCT GTGGGACCTT GGACTACTCG CCTCAGGGAG ATCTACTCAC 
     4981 CTCCAGTGGG TGGCACCAGC GCCAGATACC CAAAGCTGTA CCTCGATGGA CCATTCGGAG 
     5041 AGGGCCACCA GGAATGGCAC AAGTTTGAGG TGTCGGTGCT GGTAGGAGGG GGCATTGGGG 
     5101 TCACCCCCTT TGCCTCCATC CTCAAAGACC TGGTCTTCAA ATCGTCCATG GGTACCCAGA 
     5161 TGCTATGTAA GAAGATCTAC TTCATCTGGG TGACAAGGAC TCAGCGGCAG TTTGAGTGGC 
     5221 TGGCTGACAT CATCCGGGAA GTGGAGGAGA ATGGCAGCCG GGACCTGGTG TCTGTGCACA 
     5281 TCTATATCAC TCAGCTGGCT GAGAAGTTTG ACCTCAGGAC GACCATGCTG TACATCTGTG 
     5341 AGAGGCACTT CCAGAAGGTG CTGAACAGGA GTCTGTTCAC GGGCCTGCGC TCTGTCACCC 
     5401 ACTTTGGTCG CCCTCCCTTT GAGCTCTTCT TAGACTCTCT GCAGGAGGTT CATCCACAGG 
     5461 TACATAAGAT TGGTGTGTTC AGCTGTGGTC CTCCAGGGAT GACCAAGAAT GTGGAGAAGG 
     5521 CCTGTCAGCT CATCAACAGG CAGGACCGGG CCCACTTTGT GCATCATTAT GAGAACTTCA 
     5581 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5641 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5701 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5761 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5821 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5881 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5941 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     6001 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     6061 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6121 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6181 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6241 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6301 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6361 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6421 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6481 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6541 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6601 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6661 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6721 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6781 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6841 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6901 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6961 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     7021 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     7081 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7141 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7201 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7261 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7321 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7381 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7441 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7501 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7561 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7621 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7681 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7741 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7801 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7861 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7921 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7981 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     8041 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     8101 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8161 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8221 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8281 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8341 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8401 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8461 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8521 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8581 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8641 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8701 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8761 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8821 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8881 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8941 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9001 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     9061 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9121 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9181 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9241 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9301 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9361 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9421 AACGCGAATT TT
//