LOCUS       RR201744                7836 bp    DNA     circular  SYN 
DEFINITION  Eif4enif1 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1), (10 ug)
ACCESSION   NM_001107230
VERSION     NM_001107230.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3993..4084 
                     /label="Myc-DDK"  
     misc_feature    4114..4699 
                     /label="PolyA signal"  
     misc_feature    4171..4190 
                     /label="Restriction Enzyme"  
     misc_feature    4975..5647 
                     /label="ColE1"  
     misc_feature    6077..6871 
                     /label="Kan/Neo"  
     misc_feature    6906..7208 
                     /label="SV40 ori"  
     misc_feature    7294..7771 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3975..3981 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3975 
                     /label="Eif4enif1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGAGA AGTGTGGATG AAACAGAAAA TGGAGATGCT TTCCTTGACC 
     1081 TGAAGAAGCT GCCCACCTCC AAATCCCCCC ACCGCTATAC GAAAGAGGAG CTCTTGGATA 
     1141 TAAAAGAACG CCCATACTCC AAACAGAGGC CATCATGCCT CTCTGAAAAA TATGACAGTG 
     1201 ATGGTGTCTG GGACCCTGAG AAGTGGCATG CCTCTCTTTA CCCAGCGTCA GGGCGCAGCT 
     1261 CACCAGTGGA AAGTCTGAAG AAAGAGCCGG AATCAGATCG GCCTTCTCTG GTGCGCAGAA 
     1321 TAGCAGATCC ACGAGAGCGA GTGAAAGAAG ATGACTTAGA TGTTGTTCTC AGCCCACAGA 
     1381 GACGAAGTTT TGGCGGTGGC TGCCATGTAA CAGCTGCTGT TAGCTCCCGG CGCTCTGGCA 
     1441 GTCCATTGGA GAAAGACAGT GATGGGCTTC GCCTGCTTGG TGGACGAAGA ATTGGCAGTG 
     1501 GAAGAATAAT TTCTGCCCGG GCCTTTGAGA AGGATCACCG TCTTAGCGAT AAGGACCTAC 
     1561 GAGACTTGAG AGACCGAGAC AGGGAGAGGG ACTACAAGGA CAAGCGTTTC AGGAGGGAGT 
     1621 TTGGAGATAG TAAACGTGTC TTTGGTGAGC GTAGAAGAAA CGATTCATAC ACTGAGGAAG 
     1681 AACCAGAATG GTTTTCAGCT GGACCCACCA GTCAGTCTGA AACCATAGAG CTGACTGGCT 
     1741 TTGATGATAA GATTCTAGAG GAGGATCACA AAGGGAGAAA ACGAACAAGG CGACGGACTG 
     1801 CCTCTGTGAG GGAAGGCATA GTGGAGTGCA ATGGAGGGGT GGCTGAAGAG GACGAGGTGG 
     1861 AAGTCATTCT GGCCCAGGAG CCCTCTGCTG ATCAGGAAGT TCCAAGGGAT GTCATCCTAC 
     1921 CTGAGCAGTC CCCAGGAGAA TTTGACTTTA ATGAATTCTT TAACCTTGAT AAGGTGCCAT 
     1981 GCTTGGCTTC GATGATAGAA GATGTTCTGG GAGAAGGATC AGTCTCTGCC AGCCGCTTCA 
     2041 GTAGGTGGTT CTCTAACCCA AGCCGGTCAG GGAGCAGATC CAGCAGCCTC GGATCCACAC 
     2101 CCCATGAAGA ACTTGAGAGA CTGGCAGGTT TGGAGCAAGC CGTCCTCTCT CCTGGACAGA 
     2161 ACTCGGGGAA TTATTTTGCT CCTATACCAT CAGAAGACCA TGCTGAAAAT AAAGTGGACA 
     2221 TTTTAGAAAT GCTACAGAAA GCCAAAGTGG ATTTAAAACC CCTTCTTTCT AGCCTTTCTG 
     2281 CAAATAAAGA AAAACTTAAA GAAAGCTCAC ATTCAGGGGT TGTACTTTCA GTGGAAGAGG 
     2341 TAGAAGCCGG GCTGAAGGGC TTGAAGGTGG ACCAGCAAAT AAAGAATTCA ACTCCCTTCA 
     2401 TGGCAGAACA TTTAGAGGAG ACCCTGAGTG CTGTGACTAG CAATCGGCAG CTCAAGAAAG 
     2461 ATGGAGATAT GACTGCATTC AACAAACTAG TGAACACCAT GAAGGCAAGT GGGACTTTGC 
     2521 CTACCCAGCC CAAAGTCAGT CGAAATCTTG AAAGTCACTT GTTGGCTCCT GCTGAGATTC 
     2581 CAGGCCAGCC TGTCCCTAAA AACATCCTAC AGGAACTTCT GGGTCAACCG GTTCAGAGAC 
     2641 CTGCTTCTTC CAATCTTCTC AGTGGCCTTA TGGGGAGCTT GGAGCCTACT GCATCTTTAT 
     2701 TGAGCCAAAG AGCACCCTCT CCTCCAATGT CACAGGTGTT TCGAACTCAA GCCGCCTCAG 
     2761 CAGACTACCT TCATCCAAGG ATACCATCAC CCATTGGTTT CCCATCAGGA CCCCAGCAAC 
     2821 TACTTGGAGA TCCATTCCAA GGCATGCGCA AGCCCATGAG TCCTGTCACA GCCCAGATGA 
     2881 GCCAGCTAGA ACTGCAGCAA GCTGCTTTAG AAGGGCTGAC CCTGCCACAT GACCTTGCAG 
     2941 TACAGACCAC ACCCTTCTAC CAGCCTGGTT TTAGCAAACC TCAAGTGGAC AGAACCAGAG 
     3001 ATGGGCTCAG AAACAGGCAA CAGCGAATGA CAAAGTCACC AGCACCCATG CACCGAGGAA 
     3061 ATTCCTCTTC CCCAGCTCCC GCGGCCTCCA TCACGAGCAT GCTTTCTCCT TCCTTCACCC 
     3121 CTACCTCAGT GATCCGCAAG ATGTATGAAA GCAGAGAGAA AAGCAAGGAG GAAATAGCAC 
     3181 CCGGAACAGT GGTTCCTGGT GATGGTAAAG AGGATGCTCA GAAGGCCAGC GAAGAAAACC 
     3241 TGCTGTCATC CAATTCCATA TCCAATTCTG ATCAAGACTC TTCTCCTACG GCAAATCCTA 
     3301 AACTTTCAAC ATTACAGCGG TCTTCATGCT CCACCCCACT GTCCCAGACC AACCGTTATA 
     3361 CCAAAGAACA AGATTATCGC CCTAAAGCAG CTGGGAGGAA AACACCTACC TTGGCATCCC 
     3421 CAGTCCCAGG AACACCTTTT CTCCGCCCTA CCCACCAAGT TCCCCTCGTC CCTCATGTTC 
     3481 CTATTGTTCG ACCTGCACAT CAGCTTCACC CAGGATTAGT CCAGAGGTTG ATAGCCCAAG 
     3541 GGGTGCATCC ACAGCATCTT CCAGGTTTGC TCCAAGCTGG TGTGCTTCCT CCTGGGATAG 
     3601 ACATAGCTCC TTTACAGGGA CTATCTGGCC CACTCCTAGG TCAACCCTTG TACCCTTTGG 
     3661 TCTCTGCTGC TAGTCACCCT CTCCTAAACC CTCGTCCTGG GACCCCTCTG CATTTGGCAG 
     3721 TGATGCAGCA GCAGCTACAG CGCTCAGTTC TGCATCCTCC AGGCTCTAGT TCCCAGGCAG 
     3781 CAGCTATCAA CGTTCAGACT CCTCCGAATG TACCCAGCCG GTCAGGCCTG CCCCATATGC 
     3841 ACTCCCAGCT GGAGCATCGC ACCAGCCAAA GGAGCAGCTC CCCCGTGGGT CTTGCCAAGT 
     3901 GGTTTGGCTC AGATGTGCTA CAGCAGCCTC TGCCCTCCAT GCCCACCAAA GTCATCAGTG 
     3961 TAGATGAACT GGAATATAGA CAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4021 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4801 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4861 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4921 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4981 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5041 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5101 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5161 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5221 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5281 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5341 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5401 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5461 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5641 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5701 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5761 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5821 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5881 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5941 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6001 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6061 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6121 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6181 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6241 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6301 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6361 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6421 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6481 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6541 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6601 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6661 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6721 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6781 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6841 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6901 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6961 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7021 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7081 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7261 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7321 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7381 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7441 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7501 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7561 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7621 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7681 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7741 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7801 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//