LOCUS       RR201663                8313 bp    DNA     circular  SYN 
DEFINITION  Asap1 (Myc-DDK-tagged ORF) - Rat ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (Asap1), (10 ug)
ACCESSION   NM_001044245
VERSION     NM_001044245.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="Asap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGATCTTCA GCCTCCCGGC TCTCCAGTTT TTCCTCAAGA GATTCACTAT 
     1081 GGAATCGGAT GCCGGACCAG ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT 
     1141 ACAACTCGCC CACCACGTCC AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA 
     1201 CGCTGCTGGA GGAGGCTCTA GACCAAGATA GAACAGCCCT CCAGAAAGTG AAGAAATCTG 
     1261 TGAAAGCAAT ATATAATTCT GGTCAAGATC ATGTACAAAA TGAGGAAAAC TATGCACAAG 
     1321 TTCTGGATAA GTTTGGAAGT AATTTTTTAA GCAGAGACAA CCCTGACCTG GGCACTGCTT 
     1381 TTGTCAAGTT TTCTACACTT ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG 
     1441 GTCTGAGCCA CAATGTGATC TTCACCTTGG ATTCCTTGTT AAAAGGAGAC CTGAAGGGGG 
     1501 TCAAAGGAGA TCTCAAGAAG CCATTTGACA AAGCCTGGAA AGATTATGAG ATGAAGTTTA 
     1561 CAAAAATCGA GAAGGAGAAA AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA 
     1621 TAACAGGAGC TGAGATTGCA GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA 
     1681 TGTGTGAATA TCTCATTAAA GTTAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC 
     1741 AGAACCTCAT AAAGTATTAT CATGCACAAT GCAATTTCTT TCAGGATGGC TTGAAAACAG 
     1801 CTGATAAACT GAAACAGTAC ATTGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA 
     1861 CCCAAGATGA AGAAAAGAAA CAGTTAACTG CACTCCGAGA CCTAATAAAA TCCTCTCTGC 
     1921 AGCTTGATCC GAAAGAAGTA GGTGGTTTAT ATGTTCCCAG CAGGGCTAAC AGTGACTCTC 
     1981 AGAGCCGGCA AGGTGGCTAC AGTATGCATC AGCTCCAGGG CAACAAGGAG TATGGCAGTG 
     2041 AAAAGAAGGG CTTTCTGCTG AAGAAGAGTG ACGGGATCCG GAAAGTGTGG CAAAGAAGGA 
     2101 AGTGTGCTGT CAAGAATGGG ATACTGACCA TTTCACATGC AACTTCCAAC AGGCAGCCTG 
     2161 CTAAGTTAAA CCTCCTCACC TGCCAGGTGA AACCGAATGC TGAGGACAAG AAGTCTTTTG 
     2221 ACCTGATATC ACATAATCGA ACATATCACT TTCAGGCAGA AGACGAGCAG GACTATGTAA 
     2281 CGTGGATATC AGTACTGACG AATAGCAAAG AAGAGGCCCT AACCATGGCC TTCCGTGGTG 
     2341 AGCAGAGCAC AGGGGAGAAT AGCCTGGAGG ATCTGACCAA AGCCATCATT GAGGATGTAC 
     2401 AGCGACTCCC TGGGAATGAC ATCTGCTGTG ACTGTGGCTC ATCAGAACCC ACATGGCTTT 
     2461 CCACCAACTT GGGTATTTTG ACCTGTATAG AATGTTCCGG AATCCATAGG GAAATGGGGG 
     2521 TTCATATTTC TCGCATTCAG TCTTTGGAAC TAGACAAATT AGGAACTTCT GAACTCTTGC 
     2581 TGGCCAAGAA TGTAGGAAAC AATAGTTTTA ATGATATTAT GGAAGCAAAT TTACCCAGCC 
     2641 CTTCACCAAA GCCCACCCCT TCAAGTGATA TGACTGTACG GAAAGAGTAC ATCACTGCAA 
     2701 AGTATGTTGA CCACCGGTTC TCCAGGAAGA CCTGTGCCTC TTCCTCAGCA AAGCTGAATG 
     2761 AATTGCTTGA GGCCATCAAG TCCAGGGACT TACTTGCACT AATCCAAGTC TATGCAGAGG 
     2821 GAGTGGAGTT AATGGAGCCA CTGCTAGAAC CTGGGCAGGA GCTTGGGGAG ACAGCTCTTC 
     2881 ACCTTGCTGT CAGGACCGCA GACCAGACGT CTCTCCATCT GGTGGATTTC CTTGTCCAAA 
     2941 ACTGTGGGAA CCTGGATAAG CAGACGTCCG TGGGGAATAC GGTGTTGCAC TATTGCAGCA 
     3001 TGTACGGCAA GCCCGAGTGT CTGAAGCTGC TGCTTAGGAG CAAGCCCACC GTGGACATTG 
     3061 TTAACCAAAA TGGAGAAACT GCCTTGGATA TAGCAAAGAG ACTCAAAGCT ACGCAGTGCG 
     3121 AAGATCTGCT TTCCCAAGCT AAGTCTGGGA AGTTCAACCC GCATGTCCAT GTAGAGTACG 
     3181 AGTGGAATCT TCGACAGGAC GAGATGGATG AGAGCGATGA CGACCTGGAT GACAAACCAA 
     3241 GCCCCATCAA AAAGGAGCGC TCACCCAGAC CACAGAGCTT CTGCCACTCA TCCAGCATCT 
     3301 CACCACAGGA CAAGCTGGCA CTGCCAGGGT TCAGCACTCC ACGGGACAAA CAGCGGCTCT 
     3361 CCTACGGAGC CTTCACCAAC CAGATCTTCG TCTCTACGAG CACAGATTTG CCCACATCAC 
     3421 CCACCAGTGA GGCTCCCCCT TTACCGCCTA GGAATGCCGG GAAAGGTCCA ACTGGCCCAC 
     3481 CTTCAACACT CCCTCTAGGC ACCCAGACCT CTAGTGGCAG CTCCACCCTA TCCAAGAAGA 
     3541 GGTCTCCTCC CCCACCACCA GGACACAAGA GAACCCTGTC TGACCCTCCC AGCCCACTAC 
     3601 CTCATGGGCC CCCAAACAAA GGCGCAATTC CTTGGGGTAA CGATGTGGGC CCATCATCTT 
     3661 CAAGTAAAAC AGCAAACAAG TTTGAGGGAC TATCTCAGCA AGCAAGCACC AGTTCTGCTA 
     3721 AGACTGCCCT TGGCCCGAGA GTGCTTCCTA AACTACCTCA GAAAGTGGCA CTAAGGAAGA 
     3781 CAGAGACCAG CCATCATCTT TCCCTCGACA GAGCTAACAT CCCACCTGAG ACTTTTCAGA 
     3841 AATCATCACA GTTGTCGGAG TTACCCCAAA AGCCACCACT TGGAGACCTG CCCCCAAAGC 
     3901 CTATGGAACT GGCCCCCAAG CCCCAAATTG GAGAGTTGCC GCCTAAGCCT GGAGAGCTGC 
     3961 CCCCTAAGCC CCAATTAGGT GACCTGCCCC CCAAGCCACA GCTCTCAGAC TTACCTCCCA 
     4021 AGCCACAGAT GAAGGACCTT CCCCCCAAGC CACAGCTGGG GGACCTGCTG GCAAAGTCTC 
     4081 AGGCTAGCGA TTTGTCGGCC AAGGTGCAGC CACCCTCAGA GGTCACACAG AGGTCACACA 
     4141 CTGGGGATCT GTCTCCAAAT GTGCAGTCCA GAGATGCCAT CCAGAAGCAA GCATCTGAGG 
     4201 ACTCCAACGA CCTCACACCC ACGCTGCCAG AGACACCCGT ACCACTGCCC AGAAAAATCA 
     4261 ATACGGGGAA AAATAAAGTG AGGCGTGTGA AGACCATTTA TGACTGCCAG GCAGACAATG 
     4321 ACGATGAGCT CACATTTATT GAGGGTGAAG TGATCATTGT CACTGGGGAA GAGGACCAGG 
     4381 AGTGGTGGAT TGGGCATATC GAAGGACAGC CTGAAAGGAA GGGTGTCTTT CCAGTGTCCT 
     4441 TTGTTCACAT CCTGTCTGAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//