LOCUS       RR201629                7401 bp    DNA     circular  SYN 
DEFINITION  Rnf19a (Myc-DDK-tagged ORF) - Rat ring finger protein 19A (Rnf19a), (10 ug)
ACCESSION   NM_001130560
VERSION     NM_001130560.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3558..3649 
                     /label="Myc-DDK"  
     misc_feature    3679..4264 
                     /label="PolyA signal"  
     misc_feature    3736..3755 
                     /label="Restriction Enzyme"  
     misc_feature    4540..5212 
                     /label="ColE1"  
     misc_feature    5642..6436 
                     /label="Kan/Neo"  
     misc_feature    6471..6773 
                     /label="SV40 ori"  
     misc_feature    6859..7336 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3540..3546 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3540 
                     /label="Rnf19a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAAGAACAA GAAATAAGTT TTATTTTTAA GTATAATGAA GGACTGTGTA 
     1081 TGAACATTGA TTCTGACTCA GTTCTGATGA GCATTTTAGA CATGAGTTTA CATCAGCAAA 
     1141 TGGGTTCAGA TAGAGATCTC CAATCGTCTG CTTCGTCTGT GAGCTTGCCT TCTGTCAAAA 
     1201 AGGCACCCAA AAAGAGAAGA ATTTCAATAG GCTCTCTCTT TCGGAGGAAG AAGGACAACA 
     1261 AACGTAAATC AAGGGAGCTC ACTGGTGGGG TGGATGGAAT TGCAAGTATA GAAAGCATAC 
     1321 GTTCTGAAGT GTGTGCTGAG AAGAACTCCA TTTTCTCTAC AAACGCCTCT TCTGACAATG 
     1381 GATTGACGTC CATCAGCAAA CAAATTGGAG ACTTTATAGA ATGTCCTTTG TGCCTTTTGC 
     1441 GGCATTCTAA AGACAGATTT CCTGATATAA TGACATGTCA CCATAGATCT TGCGTGGATT 
     1501 GCCTACGACA GTACCTAAGG ATAGAAATCT CTGAAAGTAG AGTTAATATC AGTTGTCCAG 
     1561 AATGCACTGA ACGGTTTAAT CCCCATGACA TTCGCTTGAT ATTAAGTGAT GATGTCTTAA 
     1621 TGGAAAAATA TGAAGAATTT ATGCTTCGAC GGTGGCTTGT TGCAGATCCT GACTGTAGGT 
     1681 GGTGTCCGGC TCCAGACTGT GGATATGCTG TGATAGCCTT TGGATGTGCC AGCTGTCCAA 
     1741 AATTAACTTG TGGACGAGAA GGCTGTGGAA CAGAGTTTTG CTACCACTGT AAACAGATCT 
     1801 GGCATCCAAA CCAGACCTGT GATGCTGCTC GGCAGGAAAG AGCCCAGAGT TTACGCTTAA 
     1861 GAACCATACG TTCTTCATCC ATTAGTTACA GCCAGGAGTC TGGAGCAGCA GCTGATGATA 
     1921 TAAAGCCGTG TCCACGATGT GCTGCTTATA TAATAAAGAT GAACGATGGG AGCTGTAATC 
     1981 ACATGACGTG TGCTGTCTGT GGCTGTGAGT TTTGTTGGTT GTGTATGAAA GAAATCTCTG 
     2041 ATTTACATTA TCTAAGTCCC TCAGGTTGTA CTTTTTGGGG GAAGAAACCC TGGAGCCGAA 
     2101 AGAAGAAAAT CCTGTGGCAG CTGGGAACCC TGGTCGGTGC TCCCGTAGGG ATCGCTCTCA 
     2161 TAGCTGGCAT TGCCATCCCC GCCATGATCA TTGGCATTCC TGTGTACGTG GGCCGCAAGA 
     2221 TTCATAATCG CTATGAAGGC AAAGATGTTT CAAAACACAA GCGGAATTTG GCCATAGCGG 
     2281 GTGGTGTGAC ACTGTCTGTG ATCGTGTCTC CAGTGGTAGC CGCAGTGACC GTAGGCATTG 
     2341 GTGTTCCTAT CATGTTAGCT TATGTCTATG GTGTTGTCCC AATTTCCCTC TGCCGAAGTG 
     2401 GGGGCTGTGG AGTTTCTGCG GGCAATGGGA AAGGAGTCAG GATCGAATTT GATGATGAAA 
     2461 ACGACATAAA CGTTGGTGGG ACTAACACAG CCATAGATAC AACATCAGTA GCAGAAGCAA 
     2521 GGCACAACCC TAGCATAGGA GAAGGAAGTG TGGGCGGTCT GACTGGCAGC TTGAGTGCAA 
     2581 GTGGAAGCCA CATGGATCGA ATAGGCACCA TGAGAGACAA CCTGAGTGAG ACAGCCAGCA 
     2641 CCATGGCATT AGCTGGGGCC AGTATCACCG GCAGCCTGTC AGGAAGCGCC ATGGTAAACT 
     2701 GTTTCAACAG GTTGGAAGTA CAAGCCGACG TACAGAAGGA GCGGTGCAGC CTCAGTGGAG 
     2761 AATCGGGCAC AGTGAGCTTG GGGACAGTCA GTGATAATGC CAGCACCAAA GCGATGGCAG 
     2821 GGTCCATTCT GAATTCTTAC ATCCCTCTGG ACAGAGAAGG GAACAGTATG GAAGTGCAAG 
     2881 TGGATATTGA GTCAAAGCCA TTCAAGTTCC GACACCACAG TGGAAGCAGT AGTGTGGATG 
     2941 ATGGCGGCGC TGCCCGAGGC CACGCAAGCG GGTCATCCAG TGGCTTGCCT GAAGGTAAAT 
     3001 CCAGTGCTAC CAAGTGGTCC AAAGAAGCAA CAGGAGGAAA AAAGTCAAAA AGTGGTAAAC 
     3061 TGAGGAAAAA GGGTAACACG AAGATAAATG AGACCAGAGA GGACATGGAT GCACAGTTGT 
     3121 TAGAGCAACA GAGCACTAAC TCAAGTGAGT TTGAGGCGCC GTCCCTCAGT GACAGTATGC 
     3181 CGTCTGTAGC AGACTCCCAC TCGAGTCATT TTTCTGAATT TAGCTGCTCA GACTTAGAGA 
     3241 GCATGAGAAC GTCCTGTAGT CACGGTTCCA GCGATTGCCA CGCCCGCTTC ACAGCTGTGA 
     3301 ACACCCTTCC TGAGGTAGAA AATGACCGTC TGGAGAATTC ACCACACCAG TGTAGCGGTT 
     3361 CTCTGCTTTC CAAAACTGCT TCCTGTTCAG AAGTGCCCCA GCCGAGCCAT GCTGCAGATG 
     3421 AGCGAGCCAC CAGCAGTGGT GGGGGGAAGG CAGCTGTGGA TGTGTGCTTT GGCGATGCCC 
     3481 TGAGAGAGAC AAATAACAAC CACTCGCACC AGGCAGCAGA CTTGAAAGTT GCTGTTCAGA 
     3541 CTGAAATTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3601 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3661 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3721 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3781 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3841 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3901 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3961 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4021 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4081 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4141 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4201 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4261 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4321 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4381 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4441 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4501 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4561 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4621 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4681 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4741 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4801 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4861 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4921 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4981 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5041 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5101 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5161 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5221 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5281 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5341 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5401 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5461 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5521 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5581 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5641 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5701 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5761 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5821 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5881 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5941 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6001 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6061 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6121 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6181 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6241 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6301 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6361 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6421 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6481 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6541 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6601 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6661 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6721 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6781 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6841 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6901 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6961 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7021 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7081 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7141 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7201 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7261 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7321 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7381 CAAAAATTTA ACGCGAATTT T
//