LOCUS       RR201622                8529 bp    DNA     circular  SYN 
DEFINITION  Vwa5b1 (Myc-DDK-tagged ORF) - Rat von Willebrand factor A domain containing 5B1 (Vwa5b1), (10 ug)
ACCESSION   NM_001107988
VERSION     NM_001107988.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Vwa5b1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGGCCTG TTGAACTGCT TCACGGGGGC AGCCCTGCCT CTCACCGCGT 
     1081 CTGACGTCAC CTCCTACGTC AGCGGCTATG CCCTGGGCCT CACGGCCTCC CTTACCTATG 
     1141 GCAACCTGGA GGCTCAGCCC TTCCAAGGTC TCTTCGTGTA TCCGACGGAT GAGTGCACCA 
     1201 CAGTGGTTGG TTTCGAAGCC GTCATTGCTG ACCGAGTTGT GACCATACAG CTCAGAGACA 
     1261 AAGCCAAGCT GGACAGAAGC TACTTGGATG TCCAATCCGC CACTGTCACA GGGAACCTTC 
     1321 CAGAAGAGGA GATTCCCATC ACACCGGGAA AGGTGACCTT GGATGAGGAT CTGGAGCGGA 
     1381 TTCTGTTCGT GGTCAACCTG GGGACCATTG CTCCCATGGA AAATGTCACC ATCTTCATCA 
     1441 GTACCTCCTC GGAACTCCCC ACGCTACCCA GCGGGGCCGT GAGGGTCCTT CTGCCGGCCA 
     1501 TCTGTGCCCC GACCGTGCAC CCATTCTGCG CCCACAGGTT TGGCTCCTCC AGACAACAGC 
     1561 CACGAGGCAA AGACCCACAC TGCTTCGGCA CCCAGTCCAA GGACTCCTAT AAGAGGCTGT 
     1621 GTCTGGCCAC TCTCCTGGAC ACAAAGGTGA CCAACCCAAT GGAATATGAA TTCAAATTTC 
     1681 AGCTGGAAAT CCGCGGGCCA TGTCTGCTTG CAGGGGTGGA GAGTCCCACT CATGAAATCC 
     1741 GAGCAGATGC AGCCCCATCG GCCCACTCAG CCAAAAGCAT CATCATCACC TTGGCCAAAA 
     1801 AACATACCTT TGACCGGCCG GTGGAGATCC TTCTCCACCC CAGTGAGCCC CACATGCCGC 
     1861 ATGTCCTGGT GGAGAAAGGG GACATGACCC TGGGGGAATT CTACCAGCAC TTGAAGCGAA 
     1921 AAGCAGATTT CGTTAGAGGA ACAAAGAAGG ACAACATCGC TGAGCGGAAG ACAGAAGTCA 
     1981 TCCGGAAGCG TCTCCACAAA GACATTCCCC ACCACTCAGT CATCATGCTC AACTTCTGTC 
     2041 CTGACCTCGA GTCCGTGCAG CCCAACCCCA GGAGGACCCA TGGGGAATTT ATCTTCCTCA 
     2101 TCGACAGGAG CAGCAGCATG AGCAAGACCA ACATCCAGTG CATCAAGGAG GCCATGTTGG 
     2161 TGGCCCTTAA GAGCCTCATG CCAGCATGCC TCTTCAATGT CATTGGTTTC GGATCCACAT 
     2221 TTAAGACAGT CTTTGCTTCC AGTCAGATCT ACAATGAGGA GAACTTGGCC CTGGCATGTG 
     2281 AATGCATCCA GAGAATGCGA GCTGACATGG GAGGCACCAA CATATTCTCC CCTCTGAAGT 
     2341 GGGTCCTCCG GCAGCCACTG CGCCAGGGTC ACCCACGGCT CCTCTTCCTG ATCACCGATG 
     2401 GGTCTGTGAA CAACACCGGG AAGGTTCTGG AGCTGGTGCG GAATCATGCC TCTTCTATCA 
     2461 GGTGCTATAG CTTTGGAATT GGACCCACGG TCTGCTACAG ACTGGTAAAA GGGCTGGCGT 
     2521 CTGTATCCAA GGGCAGTGCA GAGTTCCTGA TGGAGGGGGA GCGGCTGCAA CCAAAGATGG 
     2581 TCAAATCCTT GAAGAAGGCC ATGGCCCCAG TGCTGAGCGA CGTGACCGTG GAGTGGGTTT 
     2641 TTCCTGAGAC CACGGAGGCC CTGATCTCTC CTGTCAGCAC CAGCTCCCTT TTCCCTGGGG 
     2701 AACGGCTGGT GGGATATGGC ATTGTGTGCG ATGCCTCCTT GTACATCTCC AATTCCAGAT 
     2761 CTGACAAAAG ACGAAAGTAT GGCATGCTGC ATGCTCAGGA GTCCAGCAGC TCTGTCTTCT 
     2821 ACCACTCTCA GGACGAGGGA CTCAGGCCAG GCAGCGGAAA CTGTGCCAAG AACTTCAACC 
     2881 AGGGGCAGAC CAAAGACGCC CATCCATGCA ATGGAGACTC CCTCTCCCAT CACGGTCTGG 
     2941 ATGGCTCCCG GCGTCGGAGG GCATACAGCA CTAATCAGAT CTCCAGTCAC AAGGCTTGCT 
     3001 CAAGGGCCAC CACAGCCAGC GACCCCACTG GGACTACCAG GAGATACCCA CTTCGAAAAG 
     3061 CCAAGGTGCA AGACCTGGCC CATCAGAGCA GCTCAGAGAG CCAGCAGTGG CAGATTGATC 
     3121 TGCAGCCGCT ACTCACCAGA GGCCATGACC CAAGCCAGGG CCCCAGACTT CATGGCCCTG 
     3181 GAGCCCGCAG GCCTTCCCTT CTTCCTCAAG GCTGCCAGCC CATGCGGTTC TCTGGCCCGG 
     3241 AGCCCCAGGC CTGGAGCCCC GTGAGAGAGC TGGGTCTTGG CTCCGGCCGG AAGTCTGTCC 
     3301 TTAACAGCCG AAGCTCTGAG GACCTAGCCA CAGAGCCACC CCCTTTTCCC TCGACCTTGG 
     3361 AACCAGAGAC ATCCTCAGAC TTGGAGCCCA CGGCTGAATT AGAGGAGCAA GCTCCCTACA 
     3421 GGACCGCCAC GCCAAGCCCT GTGCTGGGCA AAGTTTTGGT CAAAGGAGTG TGTGACAACC 
     3481 AGCATATGCA GTGGGAAGTG AGCTTTGAGC TAGAACCTCC AGCCCTGCGG AGGGAGGACA 
     3541 CACAGAACGA TGACATGTGG GGCGAGACTT TCCACCACCT GGCGGCCCGC GCCATCATCC 
     3601 GCGAATTCGA GCATCTGGCC CAGCGCGAGG ATGAGATTGA GCTGGGGTCC AATCGCAGAT 
     3661 ACCAGGTGAA CGCCGTGCAC ACCAGCAAGG CCTGCAACGT GATCAGCAAG TACACGGCCT 
     3721 TCGTGCCGGT GGACATAAAC AAGAGGCAGT ACCTGCCCAC GGTGGTCAAG TACCACAATT 
     3781 CTGGTGCTCT ACTCAGCTTC CGGAACCTGA CCCGACAGTG GAAAGGCTTC TCTACTGGCC 
     3841 TTAGGAGGTC CCAGAGTATG CTCAGAGAAC ACTCCTCAGC TGCAGGAGAC AGCAAATTCC 
     3901 AGACCCTAGC CTTACATGAA AGCCTTACTT CAACCTTCAA TAAGACCCCA TCCCCTGGGC 
     3961 ATGAGAAGTA TGCTACAGCA GAAGGTCCCC CTCAAAACCT GTCCACCAGC TCTCTGTCCT 
     4021 CCACAAAGGC CTCCGAGGCC ATCTTTGGAT CTAGGTTGAT TCTCAACAAA TCCAGGCTGC 
     4081 TGACCCGAGC TACCAAAAAC TTTCTCAGCA AATCATTGCC CAAGGCTCCA GAGGTAAACC 
     4141 CAGGCAGCCA GAGCTCTGAC TACGTACCAC TGGTGTCTCT GCAGCTGGCC TCGGGAGCCT 
     4201 TTTTGCTCAA TGAAGTCTTC TGCAGGACCA TCCAAATCCC CATGGAGAAA CTCAAGTGGA 
     4261 CCTCACCCTT CAACTGCCTC CGAATGTCCC TCGTCACCCG CCGGCATGAT TTGAAGACCC 
     4321 AGAGTCCAGG GGACTGCACC AGTCTCTCCT CTCCACCTCC ACCCTCTGAC GACATCTCCC 
     4381 TAAAGTCTGG CGCCAAAGAC AGCTCAGACC AGGAGTCCAG TGAGCTGCTG GAGCACACGG 
     4441 GGAAGCTGTG GGCCACTGTG GTGGCGCTGG CGTGGCTGGA ACACAGCTCA GCTAGTTACA 
     4501 TCATTGAGTG GGAGCTGGTG GCTGCCAAGG CGAGCTCGTG GGTAGAGCTG CAGGAGGTGC 
     4561 CCGAGGGCCG CACACTGAGC ACCATCAAGG CCACTGCTCG CCAGCTGTTC GTGCTCCTGC 
     4621 GACACTGGGA TGAGAACCTG GAGTTCAATA TGCTCTGTTA CAACCCAAAT TATATGACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//