LOCUS       RR201522                9270 bp    DNA     circular  SYN 
DEFINITION  Col3a1 (Myc-DDK-tagged ORF) - Rat collagen, type III, alpha 1 (Col3a1), (10 ug)
ACCESSION   NM_032085
VERSION     NM_032085.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5427..5518 
                     /label="Myc-DDK"  
     misc_feature    5548..6133 
                     /label="PolyA signal"  
     misc_feature    5605..5624 
                     /label="Restriction Enzyme"  
     misc_feature    6409..7081 
                     /label="ColE1"  
     misc_feature    7511..8305 
                     /label="Kan/Neo"  
     misc_feature    8340..8642 
                     /label="SV40 ori"  
     misc_feature    8728..9205 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5409..5415 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5409 
                     /label="Col3a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCTTT GTGCAATGTG GGACCTGGTT TCTTCTCACC CTGCTTCACC 
     1081 CCTCTCTTAT TTTGGCACAG CAGTCCAATG TAGATGAATT GGGATGCAAC TACCTTGGTC 
     1141 AGTCCTATGA GTCTAGAGAT GTCTGGAAGC CAGAACCATG TCAAATATGT GTCTGCGACT 
     1201 CGGGATCTGT CCTCTGTGAT GACATAATGT GTGATGATGA GCCACTAGAC TGCCCCAACC 
     1261 CAGAGATCCC ATTTGGAGAA TGTTGTGCAA TATGTCCACA GCCTTCTACA CCTGCTCCTG 
     1321 TCATTCCTGA TGGTAATAGA CCTCAAGGCC CCAAGGGAGA TCCAGGCCCT CCTGGCATTC 
     1381 CTGGGAGAAA TGGCGACCCT GGTCTTCCAG GACAACCAGG GCTCCCTGGT CCTCCTGGCT 
     1441 CCCCTGGAAT CTGTGAATCA TGTCCAACTG GTGGCCAGAA TTATTCTCCC CAATTCGACT 
     1501 CATATGATGT CAAGTCTGGA GTCGGAGGAA TGGGTGGCTA TCCTGGACCA GCTGGTCCAC 
     1561 CAGGCCCTCC AGGACCCCCT GGTTCCTCTG GACATCCTGG ATCTCCTGGT TCTCCTGGAT 
     1621 ACCAAGGTCC TCCTGGTGAA CCGGGTCAAG CTGGTCCTGC AGGCCCTCCA GGACCTCCTG 
     1681 GTGCTATTGG TCCATCTGGC CCTGCAGGAA AGGATGGAGA GTCAGGAAGA CCCGGACGAC 
     1741 CTGGAGAGCG TGGACTGCCT GGACCTCCAG GTATCAAAGG CCCAGCTGGT ATCCCTGGAT 
     1801 TCCCGGGTAT GAAAGGACAC AGAGGCTTTG ATGGACGCAA TGGAGAGAAG GGTGAAACCG 
     1861 GTGCTCCTGG ATTGAAGGGT GAAAACGGTC TTCCGGGAGA CAACGGAGCT CCTGGCCCCA 
     1921 TGGGTCCCAG AGGGGCTCCT GGTGAGCGAG GACGACCAGG TCTTCCTGGA GCCGCAGGTG 
     1981 CTCGAGGCAA TGATGGTGCT CGGGGCAGTG ACGGGCAACC AGGTCCCCCT GGTCCTCCTG 
     2041 GAACTGCAGG ATTCCCTGGA TCTCCTGGTG CTAAGGGTGA GGTTGGACCT GCAGGATCCC 
     2101 CTGGCTCAAA TGGCTCTCCA GGACAAAGAG GGGAACCTGG ACCACAAGGA CACGCTGGTG 
     2161 CTCAAGGACC TCCTGGCCCT CCTGGCAATA ATGGCAGTCC TGGTGGGAAA GGTGAAATGG 
     2221 GTCCAGCTGG CATTCCTGGA GCTCCTGGAC TCCTAGGAGC TAGGGGTCCC CCTGGACCAG 
     2281 CAGGAGCTAA TGGTGCACCG GGACAACGAG GTCCTTCAGG CGAACCGGGC AAGAATGGAG 
     2341 CAAAGGGAGA GCCAGGAGCT CGCGGTGAAC GGGGCGAAGC TGGTTCCCCA GGGATTCCAG 
     2401 GACCTAAGGG CGAAGACGGC AAAGATGGGT CACCAGGAGA ACCTGGTGCA AATGGAGTTC 
     2461 CAGGAAACCC AGGAGAAAGG GGTGCCCCTG GCTTCCGAGG ACCTGCAGGG CCAAATGGCG 
     2521 CCCCAGGAGA AAAGGGTCCT GCTGGAGAGC GTGGTGGCCC AGGTCCTGCA GGACCTAGAG 
     2581 GAGTAGCTGG AGAACCTGGC CGAGATGGAA CCCCTGGAGG TCCAGGGATA CGGGGTATGC 
     2641 CCGGAAGTCC AGGTGGACCA GGCAATGATG GGAAACCAGG ACCTCCTGGA AGTCAAGGAG 
     2701 AAAGTGGTCG CCCTGGTCCT CCTGGCCCAT CTGGCCCCAG AGGTCAGCCT GGTGTCATGG 
     2761 GTTTCCCTGG TCCTAAAGGA AATGATGGTG CACCTGGCAA AAACGGAGAA CGGGGTGGCC 
     2821 CTGGGGGTCC TGGCCTTCCA GGTCCTGCTG GAAAGAATGG GGAGACTGGA CCTCAGGGTC 
     2881 CCCCAGGACC TACTGGCGCA CCTGGTGACA AGGGAGACGC TGGACCCCCT GGTCCACAAG 
     2941 GATTACAAGG CATACCTGGT ACCAGTGGTC CTCCAGGAGA AAATGGAAAA CCAGGAGAAC 
     3001 CAGGTCCAAA GGGTGAAGCT GGAGCACCTG GAGTTCCTGG AGGCAAGGGT GATTCTGGTG 
     3061 CCCCTGGAGA ACGTGGACCC CCTGGAACCG CAGGAACCCC TGGGCTTAGA GGTGGAGCTG 
     3121 GACCCCCTGG GCCTGAGGGA GGAAAGGGCC CTGCTGGTCC CCCTGGTCCC CCTGGTACTT 
     3181 CTGGTCCTCC TGGTCTGCAA GGGATGCCAG GAGAGAGAGG AGGTCCTGGG AGTCCTGGCC 
     3241 CAAAGGGTGA AAAGGGTGAG CCAGGCGGTG CGGGTGCTGA TGGAGTTCCA GGAAAGGATG 
     3301 GGCCGAGAGG TCCTGCTGGT CCTATTGGTC CCCCTGGCCC AGCTGGTCAG CCTGGAGATA 
     3361 AGGGTGAAGG TGGTGCCCCT GGACTTCCAG GTATAGCTGG ACCTCGAGGT GGCCCTGGTG 
     3421 AGAGAGGTGA ACATGGCCCT CCAGGACCTG CTGGCTTCCC GGGTGCTCCT GGGCAGAATG 
     3481 GTGAACCAGG TGCTAAAGGA GAAAGAGGTG CCCCCGGAGA GAAAGGTGAA GGAGGCCCTC 
     3541 CTGGTGCCGC AGGACCCCCT GGTGGTTCTG GACCTGCTGG TCCTCCTGGG CCTCAAGGTG 
     3601 TAAAGGGTGA ACGGGGCAGT CCTGGCGGTC CTGGTGCTGC TGGCTTTCCC GGTGGCCGTG 
     3661 GTCTTCCTGG TCCTCCCGGC AACAATGGTA ATCCAGGGCC CCCAGGACCC AGTGGTGCTC 
     3721 CTGGCAAGGA TGGCCCTCCA GGTCCTGCAG GTAACAGTGG TTCTCCTGGA AACCCTGGAG 
     3781 TAGCTGGACC AAAAGGTGAT GCTGGCCAAC CAGGAGAGAA GGGGCCACCT GGTGCTCAGG 
     3841 GTCCTCCAGG ATCTCCAGGT CCACTTGGAA TTGCAGGGCT AACTGGAGCA CGAGGTCTTG 
     3901 CTGGACCACC AGGCATGCCA GGTCCACGGG GTAGCCCTGG ACCTCAGGGT ATCAAGGGTG 
     3961 AAAGTGGAAA GCCAGGAGCC AGTGGCCATA ATGGGGAACG TGGTCCTCCT GGACCCCAAG 
     4021 GTCTTCCTGG TCAGCCTGGT ACAGCTGGTG AGCCCGGAAG GGATGGAAAC CCTGGATCAG 
     4081 ATGGTCAGCC AGGTCGAGAC GGATCTCCTG GTGGCAAGGG TGATCGTGGT GAAAATGGTT 
     4141 CTCCTGGTGC CCCAGGCGCT CCTGGTCATC CAGGACCACC TGGTCCTGTT GGTCCATCTG 
     4201 GAAAAAATGG TGACAGAGGA GAAACGGGTC CTGCTGGTCC TTCTGGTGCT CCAGGTCCTG 
     4261 CTGGAGCTCG GGGTGCTCCT GGTCCCCAAG GTCCACGAGG TGACAAAGGT GAAACTGGTG 
     4321 AACGTGGCTC TAATGGCATC AAAGGACATC GAGGATTCCC TGGCAATCCA GGTCCCCCAG 
     4381 GTTCTCCCGG TGCTGCTGGA CACCAGGGTG CAGTTGGTAG TCCAGGCCCT GCAGGTCCCA 
     4441 GAGGCCCAGT TGGTCCACAT GGACCTCCTG GAAAAGATGG ATCAAGTGGA CATCCAGGTC 
     4501 CCATTGGACC ACCAGGGCCT CGAGGTAACA GAGGTGAAAG AGGATCTGAG GGCTCGCCCG 
     4561 GTCACCCTGG ACAGCCAGGA CCCCCTGGAC CCCCTGGTGC CCCTGGTCCC TGCTGTGGTG 
     4621 GCGGTGCTGC CATTGCTGGA GTTGGAGGTG AAAAGTCTGG CGGCTTTTCA CCATATTACG 
     4681 GGGATGATCC AATGGATTTC AAGATCAACA CTGAGGAGAT CATGTCTTCA CTCAAGTCTG 
     4741 TTAACGGACA AATAGAGAGT CTTATCAGCC CTGATGGTTC TAGAAAAAAC CCTGCTCGGA 
     4801 ATTGCAGAGA CCTGAAATTC TGCCACCCTG AACTCAAGAG CGGAGAATAC TGGGTTGATC 
     4861 CTAACCAAGG CTGCAAGATG GATGCTATAA AAGTGTTCTG TAATATGGAA ACCGGAGAAA 
     4921 CATGCATAAA TGCCAGTCCC ATGACTGTCC CGCGGAAGCA CTGGTGGACA GATGCTGGTG 
     4981 CTGAGAAGAA ACATGTTTGG TTTGGAGAAT CTATGAATGG TGGCTTTCAG TTCAGCTATG 
     5041 GCAATCCTGA TCTTCCTGAA GATGTCCTTG ATGTACAGCT GGCCTTCCTC AGACTTCTTT 
     5101 CCAGCCGGGC CTCCCAGAAC ATTACATACC ACTGCAAAAA CAGCATTGCC TACATGGATC 
     5161 AGGCCAATGG CAATGTAAAG AAGTCTCTGA AGCTGATGGG ATCCAATGAG GGAGAATTCA 
     5221 AGGCTGAAGG AAATAGCAAA TTCACTTACA CAGTTCTAGA GGATGGCTGC ACTAAACACA 
     5281 CTGGTGAATG GAGCAAGACA GTCTTTGAAT ATCAAACACG CAAGGCCATG AGACTCCCCA 
     5341 TCATAGATAT CGCACCCTAT GACATTGGTG GTCCTGATCA AGAATTTGGT GTGGACATTG 
     5401 GCCCTGTTTG CTTTTTAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5461 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5521 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5581 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5641 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5701 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5761 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5821 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5881 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5941 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6001 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6061 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6121 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6181 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6241 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6301 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6361 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6421 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6481 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6541 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6601 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6661 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6721 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6781 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6841 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6901 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6961 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7021 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7081 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7141 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7201 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7261 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7321 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7381 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7441 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7501 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7561 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7621 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7681 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7741 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7801 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7861 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7921 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7981 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8041 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8101 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8161 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8221 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8281 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8341 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8401 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8461 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8521 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8581 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8641 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8701 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8761 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8821 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8881 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8941 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9001 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9061 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9121 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9181 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9241 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//