LOCUS       RR201491                9450 bp    DNA     circular  SYN 
DEFINITION  Slit3 (Myc-DDK-tagged ORF) - Rat slit homolog 3 (Drosophila) (Slit3), (10 ug)
ACCESSION   NM_031321
VERSION     NM_031321.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5607..5698 
                     /label="Myc-DDK"  
     misc_feature    5728..6313 
                     /label="PolyA signal"  
     misc_feature    5785..5804 
                     /label="Restriction Enzyme"  
     misc_feature    6589..7261 
                     /label="ColE1"  
     misc_feature    7691..8485 
                     /label="Kan/Neo"  
     misc_feature    8520..8822 
                     /label="SV40 ori"  
     misc_feature    8908..9385 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5589..5595 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5589 
                     /label="Slit3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCGGG CGGACCGGGG CCGGCGCCGC TGTGCGCGCC CGCCTGGCGC 
     1081 TGGCCTTGGC GCTGGCGAGC ATCCTGAGCG GACCCCCCGC TGCTGCCTGC CCCACCAAGT 
     1141 GTACCTGCTC CGCCGCTAGC GTGGACTGCC ACGGGCTGGG CCTGCGCGCC GTTCCCCGGG 
     1201 GCATCCCCCG CAACGCTGAG CGCCTTGACC TGGACAGAAA TAACATCACC AGGATCACCA 
     1261 AAATGGACTT CACCGGCTTG AAGAATCTCC GAGTCTTGCA TCTGGAAGAC AATCAGGTCA 
     1321 GCGTCATCGA GAGAGGCGCC TTCCAGGATC TGAAGCAGCT GGAGCGATTA CGTCTGAACA 
     1381 AGAACAAGCT CCAGGTCCTT CCAGAATTAC TTTTCCAGAG CACACCGAAG CTCACCAGAC 
     1441 TAGATCTGAG CGAAAACCAG ATCCAGGGCA TCCCGAGGAA GGCGTTCAGG GGCGTCACGG 
     1501 GCGTGAAGAA CCTGCAACTG GACAATAACC ACATCAGCTG CATTGAAGAT GGAGCCTTCC 
     1561 GAGCGCTGCG CGATTTGGAG ATCCTCACCC TTAACAACAA CAACATCAGC CGTATCCTGG 
     1621 TCACCAGCTT CAACCACATG CCGAAGATCC GGACCCTGCG TCTCCACTCC AACCACCTGT 
     1681 ACTGTGATTG TCACTTGGCC TGGCTCTCAG ACTGGCTGCG ACAGCGGCGG ACCATTGGCC 
     1741 AGTTCACCCT CTGCATGGCG CCCGTGCACC TGAGAGGCTT CAGTGTAGCA GACGTGCAGA 
     1801 AGAAGGAGTA TGTGTGTCCA GGTCCCCACT CAGAGGCTCC AGCCTGCAAT GCCAACTCCC 
     1861 TCTCCTGCCC TTCTGCCTGC TCATGCAGTA ATAACATTGT AGACTGCCGT GGGAAGGGAC 
     1921 TGACTGAGAT CCCTGCCAAC CTGCCGGAGG GCATCGTGGA AATACGCCTA GAACAGAACT 
     1981 CCATCAAATC CATTCCTGCA GGAGCTTTCA TCCAGTACAA AAAACTGAAG CGAATAGACA 
     2041 TCAGCAAGAA TCAGATATCG GACATTGCTC CAGATGCCTT CCAGGGCCTG AAATCACTCA 
     2101 CGTCGCTGGT GCTCTATGGG AACAAGATCA CAGAGATTCC CAAAGGACTG TTTGACGGGC 
     2161 TGGTGTCCCT GCAGCTGCTC CTCCTCAATG CCAACAAGAT CAACTGCCTG CGGGTAAACA 
     2221 CCTTCCAGGA CCTACAGAAC CTCAATCTGC TCTCTCTGTA TGACAACAAG TTGCAGACTA 
     2281 TCAGCAAAGG GCTCTTTGCT CCGCTGCAGT CCATCCAGAC CCTCCACTTA GCTCAAAACC 
     2341 CGTTTGTTTG CGACTGCCAC TTGAAGTGGT TGGCCGACTA CCTCCAGGAC AACCCCATTG 
     2401 AGACGAGCGG GGCCCGCTGC AGCAGCCCAC GCCGGCTGGC CAACAAGCGC ATCAGCCAGA 
     2461 TCAAAAGCAA GAAGTTCCGC TGCTCAGGCT CGGAGGATTA TCGCAACAGA TTCAGCAGCG 
     2521 AGTGCTTCAT GGACCTAGTG TGCCCCGAGA AGTGCCGTTG TGAGGGCACC ATTGTGGACT 
     2581 GCTCCAACCA GAAGCTCTCC CGCATCCCGA GCCACCTCCC TGAATATACC ACTGACCTGC 
     2641 GACTGAACGA CAATGACATC GCTGTGCTGG AGGCCACTGG GATCTTCAAG AAGTTGCCCA 
     2701 ACCTGAGGAA AATAAACTTG AGCAATAATA GGATCAAGGA GGTGCGGGAG GGTGCGTTTG 
     2761 ATGGAGCGGC AGGCGTGCAG GAACTGATGC TGACGGGGAA CCAGCTGGAG ACCATGCACG 
     2821 GACGCATGTT CCGGGGCCTC AGCGGCCTGA AGACACTGAT GTTAAGGAGC AACCTGATCA 
     2881 GCTGCGTAAA CAATGACACC TTCGCTGGCC TAAGCTCTGT GAGACTGCTG TCCCTCTATG 
     2941 ACAATCGGAT CACCACCATC AGCCCTGGAG CCTTCACCAC GCTCGTCTCC CTGTCCACCA 
     3001 TAAACCTCTT GTCTAACCCT TTCAACTGCA ACTGTCACAT GGCCTGGCTC GGCCGGTGGC 
     3061 TGAGGAAACG CCGCATCGTA AGCGGGAACC CCAGATGTCA GAAGCCCTTT TTCCTCAAGG 
     3121 AGATTCCTAT CCAAGATGTG GCCATCCAGG ACTTCACCTG TGAAGGCAAT GAAGAGAACA 
     3181 GCTGTCAGCT GAGTCCACGC TGCCCCGAGC AGTGTACCTG TGTGGAGACA GTGGTGCGAT 
     3241 GCAGCAACAG GGGTCTCCAC ACCCTCCCCA AGGGCATGCC AAAGGACGTG ACTGAACTGT 
     3301 ACCTGGAAGG AAATCATTTA ACGGCGGTGC CCAAAGAATT GTCCACCTTC CGACAGCTGA 
     3361 CACTAATTGA CCTGAGCAAC AACAGCATCA GTATGCTGAC CAATCACACC TTCAGCAACA 
     3421 TGTCCCACCT CTCCACACTG ATCCTGAGCT ACAACCGGCT GAGATGCATC CCGGTCCATG 
     3481 CCTTCAACGG GCTAAGGTCA CTCCGAGTGC TAACCCTCCA TGGCAATGAC ATTTCCAGTG 
     3541 TTCCTGAAGG CTCCTTCAAT GATCTGACGT CCCTCTCCCA CCTGGCCCTG GGAATCAACC 
     3601 CTCTCCACTG TGACTGCAGT CTGCGTTGGT TATCAGAGTG GATAAAGGCT GGGTACAAGG 
     3661 AGCCTGGCAT TGCCAGATGC AGTAGCCCCG AGTCCATGGC AGACAGACTC CTGCTCACCA 
     3721 CCCCCACCCA CAGGTTCCAG TGCAAAGGGC CAGTGGACAT TAACATCGTG GCCAAGTGCA 
     3781 ACGCCTGCCT CTCTAGCCCA TGCAAGAACA ACGGCACTTG CAGCCAGGAT CCGGTGGAGC 
     3841 AGTACCGCTG TACCTGCCCT TACAGCTACA AGGGCAAGGA CTGCACCGTG CCCATCAACA 
     3901 CCTGCGTCCA GAACCCCTGT CAGCACGGAG GCACCTGTCA CCTCAGTGAG AGCCACAGAG 
     3961 ATGGGTTCAG CTGCTCCTGC CCCCTGGGCT TTGAGGGACA GCGGTGTGAG ATCAACCCAG 
     4021 ATGACTGTGA GGATAACGAC TGTGAAAACA GCGCCACCTG TGTGGACGGG ATCAACAACT 
     4081 ACGCATGCGT GTGCCCGCCG AACTACACAG GGGAGCTGTG TGATGAGGTG ATTGACTACT 
     4141 GCGTGCCCGA GATGAACCTC TGTCAGCACG AGGCCAAGTG CATCTCCCTG GACAAAGGAT 
     4201 TCAGGTGCGA ATGTGTCCCT GGCTACAGTG GAAAGCTGTG CGAGACAGAC AACGATGACT 
     4261 GTGTGGCTCA CAAGTGTCGC CACGGAGCCC AGTGTGTGGA CGCGGTCAAT GGCTACACGT 
     4321 GCATCTGCCC CCAGGGCTTC AGCGGGCTCT TCTGTGAGCA CCCCCCACCC ATGGTTCTGC 
     4381 TACAGACCAG CCCCTGTGAC CAGTACGAGT GCCAGAATGG GGCGCAGTGC ATCGTGGTAC 
     4441 AGCAGGAGCC CACTTGCCGC TGTCCCCCAG GCTTCGCTGG GCCCAGGTGT GAGAAGCTCA 
     4501 TCACTGTGAA TTTCGTGGGC AAGGACTCCT ATGTGGAACT GGCCTCTGCC AAGGTCCGGC 
     4561 CCCAGGCCAA CATCTCCCTG CAGGTGGCCA CTGACAAGGA CAACGGCATC CTTCTTTACA 
     4621 AGGGAGACAA CGACCCCCTG GCACTGGAGC TGTACCAGGG TCACGTGAGG CTGGTGTATG 
     4681 ACAGCCTGAG CTCCCCTCCG ACCACGGTGT ACAGTGTGGA GACGGTGAAT GATGGCCAGT 
     4741 TTCACAGTGT GGAGCTGGTG ATGCTAAACC AGACCCTGAA CCTGGTGGTA GACAAAGGAG 
     4801 CCCCCAAGAG CCTGGGGAAG CTCCAGAAGC AGCCAGCAGT GGGCATCAAC AGTCCCCTCT 
     4861 ATCTTGGAGG CATCCCCACG TCTACAGGCC TCTCAGCCTT ACGCCAGGGT GCAGACAGGC 
     4921 CGCTGGGCGG CTTCCACGGC TGTATACACG AAGTGCGCAT CAACAACGAG TTGCAGGATT 
     4981 TCAAAGCCCT CCCACCCCAG TCCCTGGGGG TCTCTCCCGG CTGCAAATCC TGCACTGTGT 
     5041 GTCGTCACGG CCTGTGTCGT TCCGTGGAGA AGGACAGCGT AGTGTGTGAG TGCCATCCGG 
     5101 GATGGACTGG TCCACTGTGT GATCAGGAAG CCCAGGACCC CTGCCTTGGT CACAGCTGCA 
     5161 GCCATGGGAC ATGCGTGGCG ACTGGCAACT CATATGTGTG CAAGTGTGCC GAGGGCTATG 
     5221 AGGGACCTTT GTGTGACCAG AAGAATGACT CTGCCAATGC CTGCTCAGCC TTCAAGTGCC 
     5281 ACCACGGGCA GTGCCACATC TCAGATCGAG GGGAGCCCTA TTGCCTGTGC CAGCCTGGCT 
     5341 TCAGTGGCAA TCACTGTGAG CAAGAGAATC CATGTTTGGG AGAGATAGTC CGTGAAGCCA 
     5401 TCCGCCGCCA GAAAGATTAT GCCTCCTGTG CCACAGCATC CAAGGTGCCC ATCATGGTAT 
     5461 GCCGCGGGGG CTGCGGGAGC CAATGCTGCC AGCCGATTCG GAGCAAGCGG CGGAAATACG 
     5521 TCTTCCAGTG CACTGACGGC TCCTCGTTCG TGGAAGAGGT GGAGAGACAC TTGGAATGTG 
     5581 GCTGTCGTGA GTGTTCCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5641 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5701 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5761 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5821 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5881 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5941 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     6001 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6061 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6121 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6181 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6241 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6301 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6361 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6421 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6481 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6541 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6601 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6661 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6721 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6781 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6841 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6901 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6961 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     7021 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7081 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7141 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7201 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7261 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7321 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7381 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7441 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7501 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7561 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7621 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7681 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7741 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7801 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7861 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7921 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7981 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     8041 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8101 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8161 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8221 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8281 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8341 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8401 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8461 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8521 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8581 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8641 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8701 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8761 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8821 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8881 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8941 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     9001 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9061 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9121 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9181 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9241 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9301 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9361 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9421 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//