LOCUS       RR201453                6917 bp    DNA     circular  SYN 
DEFINITION  Capn10 (Myc-DDK-tagged ORF) - Rat calpain 10 (Capn10), (10 ug)
ACCESSION   NM_031673
VERSION     NM_031673.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3074..3165 
                     /label="Myc-DDK"  
     misc_feature    3195..3780 
                     /label="PolyA signal"  
     misc_feature    3252..3271 
                     /label="Restriction Enzyme"  
     misc_feature    4056..4728 
                     /label="ColE1"  
     misc_feature    5158..5952 
                     /label="Kan/Neo"  
     misc_feature    5987..6289 
                     /label="SV40 ori"  
     misc_feature    6375..6852 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3056..3062 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3056 
                     /label="Capn10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGCGGTC CGGGCCGAGA CGCGGGCGCG GGAGCTCTTC CGGGACGCCG 
     1081 CATTCCCCGC CTCGGACTCC TCGCTCTTTT ACAACTTGTC CACGCCTCTG GCCCAGTTTC 
     1141 GGGAGGACAT CACTTGGAGA CGGCCCCAGG ACATTTGTGC CACACCTCAA CTGTTTCCAG 
     1201 ATAACCCATG GGAAGGACAG GTGAAGCAAG GGCTGCTGGG GGATTGCTGG TTCCTGTGTG 
     1261 CCTGTGCCGC CCTTCAGAAG AGTCGACACC TCCTGGACCA GGTCTTCCCT CCAGGACAGC 
     1321 CAGGCTGGTC TGACCAGGAA TACCAAGGCT TCTTCACCTG TCGGATTTGG CAGTTTGGAC 
     1381 ACTGGGAGGA AGTGACCATA GATGACCGTC TGCCTTGTCT TGCCGGGAGA CTCTGCTTTT 
     1441 CTCGATGCCA GAGAGAGGAT GTGTTCTGGC TTCCCTTACT GGAAAAGGCC TACGCTAAGG 
     1501 TTCATGGATC CTATGAGCAC CTGTGGGCAG GGCAGGTGGC AGATGCCCTG GTGGATCTCA 
     1561 CTGGCAGCCT GGCAGAGAGG TGGAGCCTGA AGGACATAAG GAAAGCCAGC GGCCAGCAGG 
     1621 ACAGACCCAG TGGTGGAGAG CACAGAGCCT GTCAGCAGCT ACTCCGTCTC AAGGACCAGT 
     1681 GTCTGCTCAG CTGCTCTGTG CTGAGCCCGA GAGCAGGTGC TAGGGAGCTC GGGGAGTTCC 
     1741 ATGCCTTCAT TATCTCAGAT CTGCAGGAGC TCAGGAGTCA GACTGGTCAG GGTATCCTCC 
     1801 TGCTGAGGAT TCACAACCCT TGGGGCCGGC GTTGTTGGCA GGGCCTCTGG AGAGAAGGCG 
     1861 GTGAAGGGTG GAACCAGGTA GAGCCAGCTA AGGAGTCTGA GCTGCTGGCA CAACTCCAGG 
     1921 AAGGAGAGTT CTGGGTAGAG GAAGAGGAGT TCCTCAGGGA GTTTGATGAG GTCACCATCG 
     1981 GCTACCCAGT CACAGAGGCC GGCCACCTGC AAAGTCTCTA CACAGAGAAG GTGCTGTGCC 
     2041 ATACGCGGGC ACTGCCTGGT GCCTGGGTGA CAGGGCAATC AGCTGGAGGC TGCCGGAACA 
     2101 ACAGTTGCTT TCCCTGCAAC CCCAAGTTCT GGTTACGGCT CTTGGAACCC AGTGAGGTGT 
     2161 GTGTGGCTGT CCTTCAGAGA CCCCGGAGGC GCTTAGTGGG CCAGACCCGG GCACTGGCGG 
     2221 GCGCCAGCCC TGCACCAGTG AACCTCCCAG GCAAAGACTA CCAGGCTGTG GGCCTGCACA 
     2281 TCTGGAAGGT AGAGAAACGG AAGATCAGCC TGCCCAGAGT CCTGTCTGCA CCCCCGGTGG 
     2341 CTGGCACTGC ATGCCATGCG TATGATCGTG AGATCCACTT GCGTTGTGAG CTCTCACCAG 
     2401 GCTACTACCT GGCTGTCCCT AGCACCTTTT TGAAGGATGT GCCAGGGCAG TTCCTGCTCC 
     2461 GAGTCTTCTC CACCGGGAAA ATCTCCCTCA GTGCCGTCAG GCTGGCCACC AAGGGTGCAT 
     2521 CACCTGGAGC AGCCCTGCCT GCAGGCGAGT GGGAGACTGT GCAGTTGCAG GGCAGCTGGA 
     2581 GAGCTGGCCA GACAGCCGGG GGCAGTAGAA ACTTTGCCTC TTACCCCTGC AATCCCTGCC 
     2641 TCCCCTTCTC TGTTCCTGAG GGTGCTGGTC CCCGCTACAT CCGTATCACC CTACAGCAAC 
     2701 ACTGCCGGCT CAGTGACAGC CAGCTCCACC CCATTGGTTT CCATGTCTTT CAGGTTCCAG 
     2761 CAGACGGTGA AAAACAGGAT GCGTGTTCCC TGCTGCTCCA GGAGCCACTG CTAAGCTGTG 
     2821 TACCACATTG CTATGCCCAG GAAGTAAGCC GCCTCTGCCT CCTTTCTGCG GGGAACTACA 
     2881 GGATTGTGCC CTCCACCTAC CTGCCAGATA CAGAGGGTAC CTTCACAGTG ACCATAGCAA 
     2941 CCAGAATAGA CAGACAGTCC ATTCACAGCC AGGAGATGCT GGGCCAGCTA CTCCAGGAGG 
     3001 TCTCCTTCAT GGCAGTGATG AAAGCCTACT CCAGGAGGTC TCCTTCATGG CAGTGATGAA 
     3061 AGCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     3121 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG 
     3181 CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG 
     3241 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA 
     3301 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA 
     3361 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA 
     3421 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC 
     3481 TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT 
     3541 TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT 
     3601 CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG 
     3661 CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC 
     3721 AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG 
     3781 TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG 
     3841 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 
     3901 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     3961 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     4021 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     4081 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     4141 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     4201 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     4261 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     4321 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     4381 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     4441 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     4501 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     4561 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     4621 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 
     4681 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC 
     4741 GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA 
     4801 AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC 
     4861 CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC 
     4921 TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA 
     4981 GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC 
     5041 ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG 
     5101 GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA 
     5161 GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG 
     5221 CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA 
     5281 TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT 
     5341 CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT 
     5401 GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG 
     5461 CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA 
     5521 TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG 
     5581 GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG 
     5641 CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC 
     5701 CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG 
     5761 ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA 
     5821 AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG 
     5881 TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT 
     5941 GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG 
     6001 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG 
     6061 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG 
     6121 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC 
     6181 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC 
     6241 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 
     6301 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT 
     6361 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     6421 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     6481 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     6541 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     6601 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     6661 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     6721 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     6781 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     6841 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     6901 AATTTAACGC GAATTTT
//