LOCUS       RR201430                6350 bp    DNA     circular  SYN 
DEFINITION  Chga (Myc-DDK-tagged ORF) - Rat chromogranin A (Chga), (10 ug)
ACCESSION   NM_021655
VERSION     NM_021655.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2507..2598 
                     /label="Myc-DDK"  
     misc_feature    2628..3213 
                     /label="PolyA signal"  
     misc_feature    2685..2704 
                     /label="Restriction Enzyme"  
     misc_feature    3489..4161 
                     /label="ColE1"  
     misc_feature    4591..5385 
                     /label="Kan/Neo"  
     misc_feature    5420..5722 
                     /label="SV40 ori"  
     misc_feature    5808..6285 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    2489..2495 
                     /label="Restriction Enzyme"  
     misc_feature    1029..2489 
                     /label="Chga"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CGCTCCTCCG CGGCTTTGGC GCTTCTGCTG TGCGCCGGGC 
     1081 AAGTCTTTGC CCTTCCTGTG AACAGCCCTA TGACAAAAGG GGACACTAAG GTGATGAAGT 
     1141 GTGTCCTGGA GGTCATCTCC GACTCGCTGT CCAAACCCAG CCCCATGCCT GTCAGCCCTG 
     1201 AGTGTCTGGA GACCCTCCAA GGAGATGAGA GGGTCCTCTC CATCCTTCGA CACCAGAATT 
     1261 TGCTGAAGGA ACTTCAAGAC CTGGCGCTTC AAGGTGCCAA GGAGCGGGCC CAGCAGCAGC 
     1321 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAACA ACAACAACAC AGCAGCTTTG 
     1381 AGGATGAACT CTCGGAAGTA TTTGAGAACC AGAGCCCTGC AGCCAAGCAT GGAGACGCAG 
     1441 CATCAGAAGC CCCCTCCAAG GACACTGTAG AGAAGAGAGA GGATTCTGAC AAGGGGCAAC 
     1501 AGGATGCCTT TGAGGGAACC ACAGAAGGAC CCAGACCTCA GGCCTTTCCA GAGCCCAAGC 
     1561 AGGAGTCCTC TATGATGGGA AACAGTCAAT CTCCAGGGGA GGACACAGCC AACAATACCC 
     1621 AATCACCAAC CAGCCTCCCC AGCCAGGAGC ATGGGATTCC ACAGACCACA GAAGGCAGTG 
     1681 AGAGAGGTCC CAGTGCCCAG CAGCAAGCCA GAAAAGCCAA GCAAGAGGAG AAAGAGGAAG 
     1741 AGGAGGAGGA GAAAGAGGAA GAGGAGGAGG AGAAAGAGGA GAAGGCGATC GCCAGAGAGA 
     1801 AGGCTGGGCC TAAAGAAGTC CCCACGGCAG CATCCAGTTC TCACTTCTAT TCAGGCTACA 
     1861 AGAAGATCCA GAAAGATGAT GATGGTCAGT CGGAGTCTCA GGCAGTGAAT GGAAAGACAG 
     1921 GGGCTTCTGA AGCTGTGCCA TCTGAAGGGA AGGGGGAGCT GGAGCACTCT CAGCAGGAGG 
     1981 AAGATGGGGA AGAGGCCATG GCAGGGCCCC CCCAAGGTCT CTTCCCAGGT GGGAAGGGCC 
     2041 AGGAGCTGGA ACGTAAGCAG CAGGAGGAGG AAGAGGAAGA GGAGCGTCTG TCCAGAGAAT 
     2101 GGGAGGACAA GCGATGGAGC AGGATGGACC AGCTGGCCAA GGAGCTGACA GCAGAGAAGC 
     2161 GGCTGGAGGG GGAAGATGAC CCTGACCGGT CCATGAAGCT CTCCTTCCGG GCCCGGGCCT 
     2221 ATGGCTTCAG GGACCCTGGG CCTCAGCTAC GCCGGGGCTG GAGGCCATCT TCCAGAGAAG 
     2281 ACAGTGTGGA GGCCCGAGGC GACTTTGAGG AAAAGAAGGA AGAGGAGGGC AGCGCCAACC 
     2341 GCAGAGCAGA GGACCAGGAG CTAGAGAGCC TGTCAGCCAT CGAGGCAGAG CTGGAGAAGG 
     2401 TGGCCCACCA GCTGCAGGCT TTGCGACGGG GAGCCTGTCA GCCATCGAGG CAGAGCTGGA 
     2461 GAAGGTGGCC CACCAGCTGC AGGCTTTGCG ACGGGGAACG CGTACGCGGC CGCTCGAGCA 
     2521 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     2581 CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC 
     2641 ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA 
     2701 CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA 
     2761 ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA 
     2821 GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG 
     2881 CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT 
     2941 TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA 
     3001 CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC 
     3061 CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA 
     3121 AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC 
     3181 CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT 
     3241 AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG 
     3301 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 
     3361 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 
     3421 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 
     3481 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 
     3541 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 
     3601 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 
     3661 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 
     3721 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 
     3781 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 
     3841 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 
     3901 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 
     3961 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 
     4021 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 
     4081 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 
     4141 TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG 
     4201 CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC 
     4261 CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA 
     4321 TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG 
     4381 GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG 
     4441 AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT 
     4501 CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG 
     4561 GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA 
     4621 GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC 
     4681 GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT 
     4741 GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT 
     4801 CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG 
     4861 GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT 
     4921 CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT 
     4981 GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG 
     5041 CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC 
     5101 CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG 
     5161 CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT 
     5221 CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA 
     5281 GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA 
     5341 GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA 
     5401 ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT 
     5461 CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA 
     5521 AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG 
     5581 GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT 
     5641 CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG 
     5701 CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA 
     5761 TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     5821 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     5881 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     5941 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     6001 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     6061 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     6121 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     6181 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     6241 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     6301 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//