LOCUS       RR201414                6341 bp    DNA     circular  SYN 
DEFINITION  Sil1 (Myc-DDK-tagged ORF) - Rat SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae) (Sil1), (10 ug)
ACCESSION   NM_199376
VERSION     NM_199376.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2498..2589 
                     /label="Myc-DDK"  
     misc_feature    2619..3204 
                     /label="PolyA signal"  
     misc_feature    2676..2695 
                     /label="Restriction Enzyme"  
     misc_feature    3480..4152 
                     /label="ColE1"  
     misc_feature    4582..5376 
                     /label="Kan/Neo"  
     misc_feature    5411..5713 
                     /label="SV40 ori"  
     misc_feature    5799..6276 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2480..2486 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2480 
                     /label="Sil1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCTCAG CACCTGCCTT CAACTAGGAT GGCCCCTCAA GGAATGCTGC 
     1081 TGGGGCTGCT GTTGGCTTCC TGTTTAACTT TCTGCCTCAG TTGTCAGAAC TCAAATAATT 
     1141 TTGCACTCAC CAACCCAGAG AAGAGTACCC ACGAGGACTC AGATACAAAG GAAACCAGAA 
     1201 GAGAGGAGGA GCTGGATGCC GAAGTCCTAG AGGTGTTAAA CCCAACCCAA GAGTGGCAGG 
     1261 CTCTTCAACC AGGACAGGCT GTTCCTGCAG GATCCCATGT GCGAATGAAC CTACAGACTG 
     1321 GAGTAAACGA GGTGAAACTC CAACAAGAAG ACAAATTCCA AAGTAATTGG AAAGGATTTA 
     1381 AAAGAGGCAG AAGGCTGGAC ATCAATACCA ACACCTACAC GTCTCAGGAC CTCAAGAGTG 
     1441 CACTGGCCAA GTTCAAGGAG GGGACAGAGA TGGAGAATTC AAAGGATGAA CTGGCAAGAC 
     1501 AGGCCACGGT AAAGCAGCTC TTCCGCCCCA TCGAGGAGCT GAAGAAGGAA TTTGATGAGC 
     1561 TGAATGTTGT TCTTGAGACT GACATGCAGA TTATGGTTCG GCTGATAAAC AAGTTCAACA 
     1621 GCTCCAGCTC TAGCCTGGAA GAGAAGGTTG CTGCACTCTT TGATCTTGAG TACTATGTCC 
     1681 ATCAGATGGA TAACGCACAG GACCTGCTGT CCTTTGGTGG CCTTCAAGTG GTGATTAATG 
     1741 GGCTCAACAG CACAGAACCC CTGGTGAAGG AGTATGCTGC TTTTGTGCTG GGGGCCGCCT 
     1801 TTTCCAGCAA TCCAAAGGTC CAGGTGGAAG CCATTGAAGG AGGAGCTCTG CAGAAGCTGC 
     1861 TGGTCATCCT GGCCACAGAG CAGCCTCTCC CCGCCAAGAA GAAGGTCCTG TTTGCACTGT 
     1921 GCTCTTTACT TCGCCACTTC CCCTACGCCC AGCAGCAGTT CCTGAAGCTC GGGGGGCTGC 
     1981 AGGTCCTCAG GAGCCTGGTG CAGGAGAAGA GTGCGAAGGT ACTGGCCGTG CGAGTGGTCA 
     2041 CCTTGCTCTA TGACCTGGTC ACCGAGAAGA TGTTTGCTGA GGAGGAGGCT GAGCTGACCC 
     2101 AGGAGTCCTC CCCAGAGAAG CTGCAGCAGT ACCGGCAGGT GCAGCTCCTG CCCGGCCTGC 
     2161 GGGAACAGGG CTGGTGTGAG ATCACGGCCC AGCTCCTGGC CCTTCCAGAG CACGACGCTC 
     2221 GGGAGAAGGT GCTGCAGACG CTGGGCGCCC TCCTCGCCAC CTGCCGGGAC CGCTACCGTC 
     2281 AAGACCTGGA GCTGAGCCGG ACACTGGGCA GTCTCCAGGC AGAGTACCAA GCCCTGGCCT 
     2341 CCCTGGAGCT CCAAGAAGGT GAAGATGACG GCTACTTCCG GGAACTGCTG GCCTCCATCG 
     2401 ACAGCTTGGT GAAGGAGCTA AGAGTGAAGA TGACGGCTAC TTCCGGGAAC TGCTGGCCTC 
     2461 CATCGACAGC TTGGTGAAGG AGCTAAGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT 
     2521 CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT 
     2581 TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG 
     2641 ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG 
     2701 TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG 
     2761 GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG 
     2821 GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG 
     2881 CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT 
     2941 GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG 
     3001 CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC 
     3061 TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT 
     3121 ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC 
     3181 ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG 
     3241 TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 
     3301 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 
     3361 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 
     3421 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 
     3481 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 
     3541 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 
     3601 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 
     3661 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 
     3721 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 
     3781 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 
     3841 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 
     3901 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 
     3961 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 
     4021 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 
     4081 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 
     4141 CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC 
     4201 GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT 
     4261 CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC 
     4321 GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC 
     4381 GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC 
     4441 ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC 
     4501 AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT 
     4561 TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA 
     4621 AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC 
     4681 ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT 
     4741 CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA 
     4801 TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG 
     4861 CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT 
     4921 CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT 
     4981 GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA 
     5041 TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT 
     5101 CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG 
     5161 GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG 
     5221 CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA 
     5281 CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA 
     5341 CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC 
     5401 ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC 
     5461 TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT 
     5521 CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT 
     5581 AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT 
     5641 GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA 
     5701 CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC 
     5761 TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     5821 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     5881 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     5941 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     6001 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     6061 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     6121 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     6181 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     6241 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     6301 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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