LOCUS       RR201412                7345 bp    DNA     circular  SYN 
DEFINITION  Nell2 (Myc-DDK-tagged ORF) - Rat NEL-like 2 (chicken) (Nell2), (10 ug)
ACCESSION   NM_031070
VERSION     NM_031070.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3502..3593 
                     /label="Myc-DDK"  
     misc_feature    3623..4208 
                     /label="PolyA signal"  
     misc_feature    3680..3699 
                     /label="Restriction Enzyme"  
     misc_feature    4484..5156 
                     /label="ColE1"  
     misc_feature    5586..6380 
                     /label="Kan/Neo"  
     misc_feature    6415..6717 
                     /label="SV40 ori"  
     misc_feature    6803..7280 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3484..3490 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3484 
                     /label="Nell2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACGCCATG GAATCCCGGG TATTACTGAG AACGTTCTGC GTGATCCTCG 
     1081 GGCTCGAAGC GGTTTGGGGA CTTGGTGTGG ACCCCTCCCT ACAGATTGAC GTCTTATCAG 
     1141 AGTTAGAACT TGGGGAGTCC ACAGCTGGAG TGCGCCAAGT CCCAGGACTG CATAATGGGA 
     1201 CGAAAGCCTT CCTCTTCCAA GATTCTCCCA GAAGCATAAA AGCACCCATT GCTACAGCTG 
     1261 AGCGGTTTTT CCAGAAGCTG AGGAATAAAC ACGAGTTCAC AATTCTGGTG ACCCTGAAAC 
     1321 AGATCCACTT AAATTCGGGA GTCATTCTCT CCATCCACCA CTTGGATCAC AGGTACCTGG 
     1381 AACTGGAAAG CAGCGGCCAC CGGAATGAGA TCAGACTGCA TTACCGCTCT GGAACTCACC 
     1441 GCCCGCACAC GGAAGTGTTT CCTTACATTT TGGCTGATGC CAAGTGGCAC AAGCTCTCCT 
     1501 TAGCCTTCAG TGCCTCCCAC TTAATTTTAC ACATCGACTG CAACAAGATC TATGAACGAG 
     1561 TGGTGGAAAT GCCTTCTACA GACTTGCCTC TGGGCACCAC ATTTTGGTTG GGACAGAGAA 
     1621 ATAACGCACA CGGGTATTTT AAGGGAATAA TGCAAGATGT GCAATTACTT GTCATGCCCC 
     1681 AGGGGTTCAT CGCTCAGTGC CCGGATCTTA ATCGAACCTG TCCAACATGC AACGACTTCC 
     1741 ATGGGCTTGT GCAGAAAATC ATGGAGCTGC AGGACATTTT ATCGAAGACG TCAGCCAAGT 
     1801 TGTCTAGAGC TGAACAACGA ATGAACAGGC TGGATCAGTG CTACTGTGAG CGGACGTGCA 
     1861 CCATGAAGGG AACCACCTAC CGGGAGTTCG AGTCCTGGAC AGACGGCTGC AAGAACTGCA 
     1921 CATGCTTGAA TGGGACCATC CAGTGCGAGA CTCTGGTCTG CCCTGCTCCC GACTGCCCGG 
     1981 CTAAATCGGC TCCAGCGTAC GTGGATGGCA AGTGCTGTAA GGAGTGCAAG TCCACCTGCC 
     2041 AGTTCCAGGG GCGGAGCTAC TTTGAGGGAG AAAGGAGCAC AGTCTTCTCA GCTTCCGGAA 
     2101 TGTGCGTCTT GTATGAATGC AAGGATCAGA CCATGAAGCT TGTTGAGAAC GCCGGCTGCC 
     2161 CGGCTTTAGA TTGCCCCGAG TCTCATCAGA TCGCCTTGTC TCACAGCTGC TGCAAGGTTT 
     2221 GCAAAGGTTA TGACTTCTGT TCTGAGAAGC ATACATGCAT GGAGAACTCA GTCTGCAGGA 
     2281 ACCTGAACGA CAGGGCAGTG TGCAGCTGCC GGGATGGTTT CCGGGCCCTC CGGGAGGACA 
     2341 ATGCCTACTG TGAAGACATT GACGAGTGTG CAGAGGGGCG CCATTACTGC CGTGAGAACA 
     2401 CCATGTGTGT GAACACACCG GGCTCTTTCC TGTGTATCTG CCAAACAGGG TACATCAGAA 
     2461 TCGACGATTA CTCGTGTACG GAACATGACG AGTGCCTCAC AAACCAGCAC AATTGTGACG 
     2521 AGAACGCTTT GTGCTTTAAC ACCGTTGGAG GTCACAACTG CGTCTGCAAG CCTGGCTACA 
     2581 CTGGGAATGG AACCACGTGC AAAGCTTTCT GCAAAGACGG CTGCAGAAAC GGAGGTGCCT 
     2641 GCATTGCTGC CAATGTCTGT GCTTGCCCAC AAGGCTTCAC CGGACCCAGC TGTGAGACAG 
     2701 ACATTGATGA GTGCTCTGAG GGCTTTGTTC AGTGTGACAG CCGTGCCAAC TGCATTAACC 
     2761 TGCCTGGGTG GTACCACTGT GAGTGCAGAG ATGGCTACCA TGACAATGGG ATGTTTGCAC 
     2821 CAGGTGGAGA ATCCTGTGAA GATATTGATG AATGTGGGAC TGGGAGGCAC AGCTGTGCCA 
     2881 ATGACACCAT TTGCTTCAAC TTGGACGGTG GCTACGATTG CCGGTGTCCC CATGGAAAGA 
     2941 ACTGCACAGG GGACTGCGTG CACGACGGGA AAGTCAAACA CAACGGCCAG ATCTGGGTGC 
     3001 TGGAGAACGA CAGGTGCTCT GTGTGTTCCT GCCAGACTGG ATTTGTTATG TGTCGACGGA 
     3061 TGGTCTGTGA CTGCGAAAAC CCCACAGTTG ACCTCTCCTG CTGCCCTGAG TGCGACCCAA 
     3121 GGCTGAGCAG CCAGTGCCTG CATCAAAACG GGGAAACCGT GTACAACAGC GGTGACACCT 
     3181 GGGTCCAGGA TTGCCGTCAG TGCCGCTGCT TGCAAGGAGA AGTTGACTGC TGGCCCCTGG 
     3241 CTTGCCCAGA GGTAGAGTGT GAATTTAGTG TCCTTCCTGA GAACGAGTGC TGCCCACGCT 
     3301 GTGTCACCGA TCCTTGTCAG GCTGACACCA TCCGCAATGA CATCACCAAA ACCTGCCTGG 
     3361 ACGAGATGAA CGTGGTTCGC TTCACTGGGT CTTCCTGGAT CAAGCACGGC ACAGAGTGCA 
     3421 CCCTCTGCCA GTGCAAGAAC GGCCACGTGT GCTGCTCAGT GGACCCACAG TGCCTCCAGG 
     3481 AGCTGGGAGC TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG 
     3541 GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC 
     3601 GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC 
     3661 TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG 
     3721 TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT 
     3781 ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC 
     3841 AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC 
     3901 GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG 
     3961 CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA 
     4021 CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG 
     4081 AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT 
     4141 CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT 
     4201 TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG 
     4261 GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     4321 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     4381 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     4441 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     4501 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     4561 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     4621 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     4681 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     4741 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     4801 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     4861 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     4921 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     4981 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     5041 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     5101 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG 
     5161 GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG 
     5221 GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA 
     5281 CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG 
     5341 TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC 
     5401 GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT 
     5461 GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG 
     5521 CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA 
     5581 ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA 
     5641 ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC 
     5701 TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG 
     5761 GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC 
     5821 ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA 
     5881 CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC 
     5941 GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA 
     6001 GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC 
     6061 GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA 
     6121 GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC 
     6181 CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT 
     6241 CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA 
     6301 GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA 
     6361 ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC 
     6421 GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG 
     6481 AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG 
     6541 AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG 
     6601 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT 
     6661 TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG 
     6721 AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC 
     6781 AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 
     6841 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 
     6901 AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 
     6961 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 
     7021 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 
     7081 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 
     7141 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 
     7201 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 
     7261 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 
     7321 TTAACAAAAA TTTAACGCGA ATTTT
//