LOCUS       RR201402                8593 bp    DNA     circular  SYN 
DEFINITION  Kcnt1 (Myc-DDK-tagged ORF) - Rat potassium channel, subfamily T, member 1 (Kcnt1), (10 ug)
ACCESSION   NM_021853
VERSION     NM_021853.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4750..4841 
                     /label="Myc-DDK"  
     misc_feature    4871..5456 
                     /label="PolyA signal"  
     misc_feature    4928..4947 
                     /label="Restriction Enzyme"  
     misc_feature    5732..6404 
                     /label="ColE1"  
     misc_feature    6834..7628 
                     /label="Kan/Neo"  
     misc_feature    7663..7965 
                     /label="SV40 ori"  
     misc_feature    8051..8528 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4732..4738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4732 
                     /label="Kcnt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCGGGCC AAGCTGCCGC GCTCACCGTC CGAGGGCAAG GCGGGTCCGG 
     1081 GGGACACCCC AGCCGGCTCT GCAGCCCCTG AGGAGCCGCA CGGACTCAGC CCGCTACTGC 
     1141 CCACCCGCGG CGGGGGCTCC GTGGGCAGCG ACGTGGGCCA GAGGCTTCAT GTGGAAGATT 
     1201 TCAGCCTGGA CTCTTCCCTT TCTCAGGTCC AGGTGGAATT CTATGTCAAT GAGAACACCT 
     1261 TCAAAGAACG CCTCAAGCTG TTCTTCATCA AAAACCAGAG ATCCAGCCTG AGGATCCGGC 
     1321 TGTTCAACTT CTCCCTCAAG CTCCTCACCT GCCTGCTGTA CATTGTCCGT GTCCTGCTCG 
     1381 ACAACCCAGA CCAGGGCATC GGATGCTGGG GCTGCACGAA GTATAACTAC ACGTTCAATG 
     1441 GTTCGTCCTC GGAGTTCCAC TGGGCTCCCA TCCTGTGGGT GGAGAGGAAA ATGGCTCTGT 
     1501 GGGTGATCCA GGTCATTGTG GCCACAATAA GCTTCTTAGA GACCATGCTC CTCATTTACC 
     1561 TCAGCTACAA AGGCAACATC TGGGAGCAGA TATTCCATGT GTCTTTCGTC TTGGAGATGA 
     1621 TCAACACACT GCCCTTCATC ATCACGGTCT TCTGGCCACC TCTCCGGAAC CTGTTCATCC 
     1681 CTGTGTTTCT CAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC AATGACTTCC 
     1741 ACCGTGCCAT CCTGCGCACA CAGTCAGCCA TGTTCAACCA GGTGCTCATC CTGTTCTGCA 
     1801 CCCTGCTGTG CCTGGTCTTC ACAGGGACCT GTGGGATTCA GCACTTAGAG CGGGCAGGTG 
     1861 GCAACTTGAA CTTGCTGACC TCCTTCTACT TCTGCATTGT GACTTTCTCA ACCGTGGGCT 
     1921 TCGGTGATGT GACACCCAAG ATCTGGCCAT CCCAGCTCCT GGTGGTCATC CTGATCTGTG 
     1981 TCACCCTTGT GGTGCTCCCA CTGCAGTTTG AAGAGCTTGT CTACCTCTGG ATGGAGCGGC 
     2041 AGAAGTCAGG GGGCAACTAT AGCCGCCACC GAGCACGGAC AGAGAAGCAT GTGGTCCTGT 
     2101 GTGTGAGCTC CCTCAAGATC GATCTCCTCA TGGATTTCCT GAATGAGTTC TATGCCCACC 
     2161 CCCGTCTCCA GGACTACTAC GTGGTCATCC TGTGTCCCTC GGAAATGGAT GTCCAGGTGC 
     2221 GCAGGGTCCT ACAGATTCCC CTGTGGTCCC AGCGTGTCAT CTACCTCCAG GGCTCTGCCC 
     2281 TCAAGGACCA GGACCTCATG CGAGCTAAGA TGGACAACGG AGAGGCCTGC TTTATCCTCA 
     2341 GCAGCAGGAA TGAGGTGGAC CGCACAGCTG CGGATCACCA GACCATCCTT CGAGCCTGGG 
     2401 CTGTGAAAGA CTTTGCCCCC AACTGCCCCC TCTATGTCCA GATTCTCAAG CCCGAAAACA 
     2461 AGTTTCACGT CAAATTTGCT GACCATGTGG TGTGCGAGGA AGAGTGCAAG TACGCCATGC 
     2521 TGGCCCTGAA CTGCATCTGC CCGGCCACCT CCACCCTCAT CACCCTGCTG GTGCACACAT 
     2581 CCCGTGGCCA GGAAGGACAG GAGTCACCAG AGCAGTGGCA GCGCATGTAC GGGCGCTGCT 
     2641 CGGGCAACGA GGTGTACCAC ATTCGCATGG GTGACAGTAA ATTCTTCCGG GAGTATGAGG 
     2701 GCAAGAGCTT CACCTACGCA GCCTTCCACG CGCACAAGAA GTATGGGGTG TGCCTCATTG 
     2761 GGCTGAAACG TGAAGAGAAC AAGAGTATCC TGCTGAACCC GGGGCCACGG CACATCCTGG 
     2821 CCGCCTCTGA CACCTGCTTC TATATCAATA TTACCAAGGA GGAGAACTCA GCTTTCATCT 
     2881 TCAAGCAGGA GGAAAAGCAG AATAGGCGGG GCCTGGCAGG GCAGGCACTT TACGAAGGGC 
     2941 CCTCCCGGCT CCCAGTGCAC AGCATCATCG CCTCTATGGT GGCCATGGAC CTGCAGAACA 
     3001 CAGATTGCCG GCCCTCCCAG GGTGGCAGTG GTGGGGGCGG CGGCAAGCTG ACTCTGCCCA 
     3061 CTGAGAACGG CTCTGGCAGT CGGCGTCCCA GCATCGCACC CGTTCTGGAG TTGGCAGACA 
     3121 GCTCAGCCCT GTTGCCCTGC GACCTGCTGA GTGACCAATC AGAGGATGAG GTGACACCCT 
     3181 CAGACGACGA GGGGCTCTCT GTGGTTGAGT ATGTGAAGGG CTACCCTCCC AACTCACCCT 
     3241 ACATTGGCAG CTCCCCGACT TTATGCCACC TCTTGCCTGT AAAGGCCCCC TTCTGCTGCC 
     3301 TGCGACTGGA TAAGGGCTGC AAACACAACA GCTATGAGGA TGCCAAGGCC TACGGGTTCA 
     3361 AGAACAAGTT GATCATCGTC TCTGCTGAGA CGGCAGGCAA CGGGCTCTAC AACTTCATCG 
     3421 TGCCTCTGCG TGCCTACTAC CGGTCCCGCC GGGAGCTCAA CCCCATTGTG CTGCTGCTTG 
     3481 ACAACAAGCC TGACCACCAC TTCCTGGAGG CCATCTGCTG CTTCCCCATG GTCTACTACA 
     3541 TGGAGGGATC CGTGGACAAC CTGGACAGCT TGCTGCAGTG TGGCATCATC TATGCTGACA 
     3601 ATCTGGTGGT GGTGGACAAG GAGAGTACCA TGAGCGCTGA AGAGGACTAC ATGGCAGATG 
     3661 CCAAGACCAT TGTCAACGTG CAGACCATGT TCAGGCTTTT CCCCAGTCTC AGCATCACCA 
     3721 CGGAGCTCAC GCACCCTTCC AACATGCGGT TCATGCAGTT CCGGGCCAAG GACAGCTACT 
     3781 CTCTGGCTCT TTCCAAACTT GAAAAGCAAG AACGGGAGAA TGGCTCCAAC CTGGCCTTCA 
     3841 TGTTCCGCCT GCCATTTGCT GCTGGTCGGG TGTTTAGTAT CAGCATGTTG GATACGCTGC 
     3901 TCTACCAGTC CTTCGTGAAG GACTACATGA TCACCATCAC CAGGCTGCTG CTGGGCCTTG 
     3961 ACACGACGCC AGGCTCTGGC TACCTCTGTG CTATGAAGGT AACTGAGGAT GACCTGTGGA 
     4021 TCCGTACTTA TGGCCGCCTC TTCCAGAAAC TCTGCTCCTC CAGCGCTGAG ATCCCCATCG 
     4081 GCATCTACAG GACCGAGTGC CATGTCTTCT CCTCTGAGCC TCATGACCTC AGAGCCCAGT 
     4141 CTCAGATCTC GGTGAACATG GAGGATTGCG AGGACACTCG GGAGGCCAAG GGGCCCTGGG 
     4201 GCACACGGGC TGCGTCTGGC GGTGGCAGCA CCCATGGCCG TCACGGGGGC AGTGCTGACC 
     4261 CGGTGGAGCA CCCACTACTG CGCCGCAAGA GCCTGCAGTG GGCCCGCAAG CTGAGCCGCA 
     4321 AGAGCAGCAA GCAGGCGGGG AAGGCACCCA TGACCACAGA CTGGATCACC CAGCAGCGAC 
     4381 TCAGCCTGTA CCGGCGCTCA GAGCGCCAGG AGCTCTCGGA GCTGGTCAAG AACCGAATGA 
     4441 AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGACGTAGC AAATTTAACA GCCAGTGATG 
     4501 TGATGAATCG GGTAAACCTG GGATATTTGC AAGATGAGAT GAATGATCAT CACCAGAACA 
     4561 CCCTTTCCTA TGTACTCATC AACCCCCCGC CAGACACAAG ACTGGAGCCC AACGATATTG 
     4621 TGTACCTCAT CCGTTCCGAC CCCCTGGCCC ACGTGACCAG CAGCTCCCAG AGTCGGAAAA 
     4681 GCAGCTGCAG CAACAAACTG TCATCCTGTA ATCCTGAGAC CCGGGATGAG ACCCAGCTCC 
     4741 ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT 
     4801 ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT 
     4861 TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT 
     4921 GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT 
     4981 GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG 
     5041 GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC 
     5101 AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC 
     5161 TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT 
     5221 TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA 
     5281 GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC 
     5341 CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA 
     5401 TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT 
     5461 CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG 
     5521 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     5581 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     5641 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     5701 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     5761 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     5821 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     5881 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     5941 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     6001 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     6061 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     6121 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     6181 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     6241 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     6301 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     6361 GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC 
     6421 CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG 
     6481 AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC 
     6541 CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC 
     6601 TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA 
     6661 GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC 
     6721 AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT 
     6781 CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC 
     6841 CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC 
     6901 GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC 
     6961 ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT 
     7021 GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT 
     7081 CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG 
     7141 CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG 
     7201 AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC 
     7261 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG 
     7321 GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC 
     7381 TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG 
     7441 CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG 
     7501 AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG 
     7561 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA 
     7621 TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA 
     7681 AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG 
     7741 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC 
     7801 TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT 
     7861 TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG 
     7921 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT 
     7981 TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT 
     8041 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 
     8101 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 
     8161 TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 
     8221 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 
     8281 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 
     8341 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 
     8401 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 
     8461 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 
     8521 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 
     8581 TAACGCGAAT TTT
//