LOCUS       RR201398                8458 bp    DNA     circular  SYN 
DEFINITION  Map4k4 (Myc-DDK-tagged ORF) - Rat mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), (10 ug)
ACCESSION   NM_001106904
VERSION     NM_001106904.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4613..4704 
                     /label="Myc-DDK"  
     misc_feature    4734..5319 
                     /label="PolyA signal"  
     misc_feature    4791..4810 
                     /label="Restriction Enzyme"  
     misc_feature    5595..6267 
                     /label="ColE1"  
     misc_feature    6697..7491 
                     /label="Kan/Neo"  
     misc_feature    7526..7828 
                     /label="SV40 ori"  
     misc_feature    7914..8391 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4588..4595 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4588 
                     /label="Map4k4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTCACC GAGGATGAAG AGGAAGAAAT CAAACTGGAG ATAAATATGC 
     1081 TGAAGAAATA TTCTCATCAT AGAAATATTG CAACATACTA TGGTGCTTTC ATTAAGAAGA 
     1141 GCCCTCCAGG ACACGATGAC CAGCTCTGGC TCGTTATGGA GTTTTGCGGG GCTGGTTCCA 
     1201 TCACAGACCT TGTGAAGAAC ACCAAAGGGA ACACGCTCAA AGAAGACTGG ATTGCTTACA 
     1261 TCTCCCGGGA AATCCTCAGG GGGTTGGCCC ATCTCCACAT TCACCATGTC ATTCACCGAG 
     1321 ACATCAAGGG GCAGAACGTG CTGCTGACTG AGAACGCGGA GGTGAAACTT GTTGATTTTG 
     1381 GTGTAAGCGC TCAGCTGGAC AGGACGGTTG GACGGAGAAA TACATTCATA GGCACACCAT 
     1441 ACTGGATGGC TCCGGAGGTC ATCGCCTGCG ACGAGAATCC AGATGCCACT TACGACTACA 
     1501 GAAGTGACCT CTGGTCTTGT GGCATCACGG CCATCGAGAT GGCAGAAGGA GCCCCCCCTC 
     1561 TCTGTGACAT GCATCCAATG AGAGCGCTGT TTCTCATCCC CAGAAACCCT CCTCCCAGGC 
     1621 TGAAGTCAAA AAAATGGTCA AAGAAATTTT TCAGCTTCAT AGAAGGCTGC CTGGTGAAGA 
     1681 ATTACATGCA GCGGCCCTCT ACAGAGCAGC TTTTAAAACA CCCTTTCATA AGGGATCAGC 
     1741 CCAATGAAAG GCAGGTTCGA ATCCAGCTTA AGGATCACAT AGACCGGACC AGGAAGAAGA 
     1801 GAGGCGAGAA AGATGAGACG GAGTACGAGT ACAGTGGCAG TGAGGAGGAG GAGGAGGAAG 
     1861 TGCCTGAGCA GGAGGGAGAG CCAAGTTCCA TCGTCAATGT GCCTGGAGAG TCGACTCTGC 
     1921 GACGTGATTT CCTGAGGCTG CAGCAAGAGA ACAAGGAACG GTCTGAGGCT CTGCGGAGAC 
     1981 AGCAGCTTCT GCAGGAGCAG CAGCTCCGGG AGCAGGAGGA GTATAAGAGG CAGCTGCTGG 
     2041 CTGAGAGGCA GAAGCGGATT GAACAGCAGA AAGAACAGAG GAGGCGGCTG GAAGAGCAAC 
     2101 AAAGGAGAGA GCGTGAAGCC AGGAGGCAGC AGGAACGTGA GCAGCGGCGG CGAGAACAGG 
     2161 AGGAGAAGAG GCGTCTGGAG GAACTGGAAA GGCGGCGTAA GGAAGAGGAA GAGAGGAGAC 
     2221 GGGCAGAAGA CGAGAAGAGG CGAGTGGAGC GGGAGCAGGA GTACATCAGG CGGCAGCTAG 
     2281 AGGAAGAGCA GCGGCACCTG GAGATCCTGC AGCAGCAGCT GCTCCAGGAG CAGGCTATGT 
     2341 TACTGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG CAGCAGGACA 
     2401 GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT GCTGACAGAG 
     2461 CTCGGGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA 
     2521 GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG 
     2581 ACCCATGTCC ACCTTCCCGC AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG 
     2641 TACCTGAGCC CACCCAAAAG GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG 
     2701 TTCCTGTGAG AACGACATCT CGTTCCCCTG TCCTGTCCCG TCGAGATTCT CCACTGCAGG 
     2761 GCAGTGGGCA GCAAAATAGC CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC 
     2821 GGCTTCTATG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG 
     2881 GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGT GGCTCTGGTG 
     2941 AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCTCAG CGCCTTGAAA 
     3001 ATGCAGCCAA AAAACCTGAA GATAAGAAAG AAGTATTTCG ACCTCTCAAA CCTGCTGTAA 
     3061 GGATTGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG CGGCCACCTC 
     3121 ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT GAGGAGGAGG 
     3181 AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC ACCAGAGCAG 
     3241 CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG ATTGTTCATG 
     3301 ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA ATCGTCCGCC 
     3361 AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT 
     3421 TTATAGACCC CAGGTTACTA CAGATCTCTC CATCTAGTGG AACAACAGTT ACTTCCGTTG 
     3481 TGGGATTTTC CTGTGATGGG CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG 
     3541 GCTCAGTGGT CAATGTGAAC CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC 
     3601 GTAAATACAA GAAGAGATTT AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAATT 
     3661 TGCTAGTTGG CACAGAGAGT GGCCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTGT 
     3721 ACCCTCTGAT CAGCCGGAGA CGGTTCCAGC AAATGGATGT ACTTGAGGGC CTGAATGTCC 
     3781 TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGCGTCTA CTATTTATCC TGGTTAAGAA 
     3841 ATAAAATACT TCATAATGAT CCGGAAGTTG AGAAGAAGCA GGGGTGGACC ACCGTGGGCG 
     3901 ACCTGGAAGG GTGCGTGCAC TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA 
     3961 TCGCTTTGAA GAGTTCTGTG GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA 
     4021 TGGCCTTTAA GTCATTTGGA GAACTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTCG 
     4081 AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG 
     4141 TGGATTCCGG CTCAGTCTAT GACATTTATC TACCAACACA CATTCAGTGT ACCATCAAAC 
     4201 CCCATGCAAT CATTATCCTC CCCAACACGG ATGGAATGGA GCTTCTGGTG TGCTACGAAG 
     4261 ATGAAGGGGT TTATGTGAAC ACGTACGGAA GAATCACCAA GGACGTGGTT CTGCAGTGGG 
     4321 GAGAGATGCC AACATCTGTA GCATACATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA 
     4381 AGGCAATAGA GATACGCTCT GTGGAAACTG GTCATTTGGA CGGTGTGTTT ATGCACAAAA 
     4441 GGGCTCAGAG ACTAAAGTTC CTGTGTGAAC GTAATGACAA GGTGTTCTTT GCTTCCGTCC 
     4501 GCTCTGGCGG CAGCAGCCAG GTCTACTTCA TGACCTTAGG CAGGACTTCC CTTCTGAGCT 
     4561 GGCATGACCT TAGGCAGGAC TTCCCTTCTG AGCTGGAGCG GACCGACGCG TACGCGGCCG 
     4621 CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG 
     4681 GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC 
     4741 CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC 
     4801 AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC 
     4861 CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC 
     4921 AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG 
     4981 GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT 
     5041 GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG 
     5101 GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC 
     5161 TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT 
     5221 GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA 
     5281 TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC 
     5341 CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT 
     5401 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 
     5461 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 
     5521 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 
     5581 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 
     5641 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 
     5701 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 
     5761 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 
     5821 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 
     5881 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 
     5941 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 
     6001 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 
     6061 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     6121 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     6181 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     6241 AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA 
     6301 GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT 
     6361 ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC 
     6421 CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA 
     6481 TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT 
     6541 GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA 
     6601 AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG 
     6661 GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC 
     6721 GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC 
     6781 GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC 
     6841 TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG 
     6901 GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC 
     6961 GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG 
     7021 CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC 
     7081 GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG 
     7141 CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG 
     7201 ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC 
     7261 GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC 
     7321 TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG 
     7381 CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA 
     7441 GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT 
     7501 CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA 
     7561 AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT 
     7621 AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG 
     7681 GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT 
     7741 GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA 
     7801 GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT 
     7861 GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG 
     7921 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 
     7981 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG 
     8041 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 
     8101 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 
     8161 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 
     8221 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 
     8281 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 
     8341 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 
     8401 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//