LOCUS       RR201329                8358 bp    DNA     circular  SYN 
DEFINITION  Sorcs2 (Myc-DDK-tagged ORF) - Rat sortilin-related VPS10 domain containing receptor 2 (Sorcs2), (10 ug)
ACCESSION   NM_001107225
VERSION     NM_001107225.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4515..4606 
                     /label="Myc-DDK"  
     misc_feature    4636..5221 
                     /label="PolyA signal"  
     misc_feature    4693..4712 
                     /label="Restriction Enzyme"  
     misc_feature    5497..6169 
                     /label="ColE1"  
     misc_feature    6599..7393 
                     /label="Kan/Neo"  
     misc_feature    7428..7730 
                     /label="SV40 ori"  
     misc_feature    7816..8293 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4497..4503 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4497 
                     /label="Sorcs2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCACCGG GGAACCCCGA GCGCCCCGAA GCGTCCTGGC CTTACTGCGC 
     1081 CGGATCGGAG CTCCCAGGTT CTGCTGCCGC CGTGCTGGCC ACGTTCGTGG CCGCTGCTGC 
     1141 TGCTGCTGCT GGTGCTGGTG GCTGCCTGCG GAGCGATGGG GCGCTCCCCC CAGCCCGGGC 
     1201 GCTGGGGTCC CAGTGTGCAG GTCACCAGGC TGCTGCCCGC GGGACGCACG GAGTCCAGCA 
     1261 ATCGCCAGGA TCCGCAGACA CGAGAATCGG AGCCTGGCGT CCCGGGTCTC CGTCCGGGTT 
     1321 CTGCTTCGGG TCCCAGCACT GATGGCGCCC CAGCTCTGGG CAAGGGGCGC AGGGTTCGGG 
     1381 CGGCGCCGGT GGCCGGTGCA GCTTCGGCTT CGCGCGCACA GGTCTCGCTC ATCAGCACGT 
     1441 CGTTCGTGCT CAAGGGAGAC GCGACGCACA ACCAGGCGAT GGTACACTGG ACAGGAGAGA 
     1501 ACAGCAGCGT CATCCTGATC CTGACGAAGT ACTACCATGC AGACATGGGG AAGGTTCTGG 
     1561 AAAGTTCTCT CTGGAGGTCC TCAGATTTTG GGACAACCTA CACCAAGCTT ACTCTCCAAC 
     1621 CTGGTGTCAC CACTGTCATT GACAACTTCT ATATCTGCCC TGCTAACAAA AGAAAGATCA 
     1681 TCCTGGTGAG CTCATCCCTC GGTGACAGGG AACAGAGCCT CTTCCTCAGC ACTGACGAGG 
     1741 GCACCACATT CCAGAAGTAT CCTGTCCCCT TCCTTGTGGA GACTCTCCTC TTTCACCCAA 
     1801 AGGAGGAGGA CAAGGTTCTT GCCTACACCA AGGACAGCAA GCTGTATGTG TCGTCCGACC 
     1861 TGGGGAAGAA GTGGACACTG CTGCAGGAAC AGGTGACCAA GGACCACGTG TTCTGGGCTG 
     1921 TGTCTGGGGT GGATGACGAC CCCAACCTGG TCCACATGGA AGCACGGGAC CTCAGTGGAG 
     1981 GCTATCGGTA CTACACGTGT CTGATCCACA ACTGCTCAGC CCAGCCACAC ACAGCACCCT 
     2041 TCCCTGGCCC CACTGACCGA GGGTCCCTGA CCGTGCAGGA TGAGTACATC TTTTTGAAGG 
     2101 CGACATCAAC AAACCGAACC AAATACTATG TCTCTTATCG CCGCAGCGAC TTTGTCCTGA 
     2161 TGAAGCTGCC CAAGTATGCC CTGCCCAAGG ACCTACAGAT CATCAGCACA GATGAGCAGC 
     2221 AGGTGTTCGT GGCCGTCCAG GAGTGGAACC AGGTGGACAC CTACAACCTG TACCAGTCGG 
     2281 ACCTGCGTGG AGTGCGCTAT TCGCTGGTGC TGGAGAACGT GCGCAGCTCG AGGCAGGCTG 
     2341 AGGAGAATGT GGTCATCGAC ATCCTTGAGG TCAGAGGGGT GAAGGGAGTC TTCTTGGCAA 
     2401 ACCAGAAAGT GGATGGCAAG GTGACAACAG TCATAACCTA CAACAAGGGC CGTGACTGGG 
     2461 ATTACCTGAG GCCACCCAGC ACTGACATGA ATGGGAAACC TACCAACTGC CAGCCACCGG 
     2521 ACTGCCACCT GCACCTGCAC CTGCGCTGGG CAGACAACCC CTATGTGTCA GGCACGGTAC 
     2581 ACACCAAGGA CACTGCCCCT GGCCTCATCA TGGGTGCAGG TAACCTGGGC TCACAGTTGG 
     2641 TGGAGTATAA AGAGGAAATG TACATCACAT CTGACTGCGG GCACACCTGG AGACAGGTCT 
     2701 TTGAGGAAGA GCACCATGTC CTGTACCTGG ACCACGGTGG TGTCATTGCT GCCATCAAGG 
     2761 ACACCTCCAT CCCCCTGAAG ACCCTCAAGT TCAGCGTGGA CGAGGGCCAC ACTTGGAGTA 
     2821 CACACAACTT CACCAGCACC TCAGTGTTCG TGGATGGGCT GCTGAGCGAA CCAGGGGATG 
     2881 AAACGCTGGT CATGACGGTC TTTGGTCACA TCAGCTTCCG CTCTGACTGG GAGCTGGTCA 
     2941 AGGTGGACTT CCGGCCCTCC TTCCCCAGGC CATGTGTTGA GGATGACTAT AGCTCCTGGG 
     3001 ACCTCACTGA CCTCCAGGGT GACCACTGTA TCATGGGCCA GCAGAGAAGT TACCGGAAGA 
     3061 GAAAGTCCAC GTCCTGGTGT GTCAAGGGGA GGAGCTTCAC GTCAGCACTC ACATCCCGTG 
     3121 TGTGCAAGTG CCGGGACTCT GACTTCCTCT GTGACTACGG GTTTGAGCGC TCATCTTCCT 
     3181 CAGAGTCTAC TGTCAACAAG TGCTCTGCCA ACTTCTGGTT CAACCCCCTG TCTCCTCCTG 
     3241 AAGACTGTGT CCTAGGACAG ACCTATACCA GTAGCCTTGG GTACCGGAAG GTGGTGTCCA 
     3301 ATGTGTGCGA AGGTGGTGTG GACCTGCAGC AGAGCCCAGT GCAGCTGCAG TGCCCCCTAC 
     3361 AAGCACCGCG GGGCCTGCAG GTGAGCATCC GAGGAGAGGC AGTGGCTGTG CGACCTAGAG 
     3421 AAGATGTGCT GTTTGTGGTG CGACAGGAAC AGGGTGATGT CCTGACCACT AAGTATCAGG 
     3481 TGGATCTTGG GGACGGCTTC AAGGCCATGT ACGTGAACCT CACTCTGACA GGCGAGCCCA 
     3541 TTCGGCACCA CTACGAGAGT CCTGGCATCT ACCGTGTGTC TGTCAGGGCT GAGAACATGG 
     3601 CAGGCCACGA TGAGGCTGTG CTCTTTGTCC AGGTCAACTC TCCCCTGCAG GCCCTCTACC 
     3661 TCGAAGTAGT CCCTGTCATT GGCATCAACC AAGAGGTGAA CCTCACAGCT GTGCTGTTAC 
     3721 CGCTGAACCC CAATCTCACT GTCTTCTACT GGTGGATCGG CCACAGCTTG CAGCCTCTGC 
     3781 TTTCCTTGGA CAACTCCGTG ACAACAAGGT TTACAGATGC TGGGGATGTG CGTGTGACAG 
     3841 TGCAAGCTGC CTGTGGGAAC TCGGTGCTCC AGGACTCCAG GCTCGTCCGT GTGCTCGATC 
     3901 AGTTCCAGGT GGTGCCTCTG CAGTTCTCCA GGGACCTGGA CACCTTTAAC CCCAACACGC 
     3961 CTGAATGGAG GGAGGACGTG GGATTGGTGG TCACCCGACT TCTCTCCAAG GAGACCAGCA 
     4021 TCCCAGAGGA GCTACTAGTG ACTGTGGTCA AGCCAGGGCT GCCCACCATA GCGGATCTGT 
     4081 ATGTGCTTCT GCCTCCTCCC AGGCCCACAA GGAAGAGGAG CCTCACAAGT GACAAGAGGC 
     4141 TGGCAGCTGT GCAGCAGGCA CTGAACTCCC ACAGGATCAG CTTCATCCTT CGAGGAGGGC 
     4201 TTCGTGTCCT GGTGGAGCTG AGGGACACAG ATGCAGGTCC TCAGAGGCCG GGTGGCAGTG 
     4261 GTGGCTACTG GGCTGTGGTG GTCCTCTTTG TCATCGGGCT CTTTGCAGTA GGAGCATTCA 
     4321 TCCTCTACAA GTTCAAAAGG AAGCGCCCAG GCAGGACGGT GTACGCCCAG ATGCACAATG 
     4381 AGAAGGAACA GGAGATGACA AGTCCCGTGA GCCACAGTGA GGACGCCCAG AGCGCCATGC 
     4441 AAGGCAACCA CTCAGGTGTG GTCCTGAGCA TCAACTCTCG AGAGATGCAT AGCTACCTGG 
     4501 TGGGCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4561 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4621 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4681 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4741 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4801 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4861 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4921 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4981 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5041 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5101 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5161 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5221 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5281 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5341 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5401 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5461 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5521 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5581 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5641 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5701 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5761 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5821 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5881 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5941 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6001 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6061 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6121 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6181 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6241 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6301 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6361 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6421 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6481 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6541 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6601 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6661 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6721 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6781 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6841 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6901 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6961 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7021 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7081 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7141 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7201 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7261 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7321 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7381 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7441 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7501 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7561 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7741 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7801 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7861 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7921 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7981 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8041 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8101 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8161 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8221 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8281 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8341 AAATTTAACG CGAATTTT
//