LOCUS       RR201298                7158 bp    DNA     circular  SYN 
DEFINITION  Fign (Myc-DDK-tagged ORF) - Rat fidgetin (Fign), (10 ug)
ACCESSION   NM_001106484
VERSION     NM_001106484.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3315..3406 
                     /label="Myc-DDK"  
     misc_feature    3436..4021 
                     /label="PolyA signal"  
     misc_feature    3493..3512 
                     /label="Restriction Enzyme"  
     misc_feature    4297..4969 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Kan/Neo"  
     misc_feature    6228..6530 
                     /label="SV40 ori"  
     misc_feature    6616..7093 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3297..3303 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3297 
                     /label="Fign"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCAGTAGC ACCAGTGTTT ATGGCTTGAA GATGCAGTGG ACGCCGGAGC 
     1081 ATGCCCAGTG GCCAGAACAG CACTTTGATA TCACCTCGAC TACTCGGTCT CCCGCCCACA 
     1141 AAGTTGAAGC CTATAGAGGT CATTTGCAAC GCACCTACCA GTATGCCTGG GCGAATGATG 
     1201 ACATATCTGC TCTGACTGCA TCCAACCTTC TGAAAAAATA TGCAGAGAAA TATTCTGGCA 
     1261 TTTTGGAAGG TCCCGTAGAC CGACCTGTAC TCAGCAATTA TTCAGACACA CCGTCGGGAC 
     1321 TAGTGAATGG TCGGAAAAAT GATAGCGAAC CCTGGCAGTC TTCCTTGAAT TCAGAAGCCG 
     1381 TTTATCCTAT GAACTGTGTT CCGGATGTCA TCACCGCCAG CAAAGCTGGT GTCAGTTCAG 
     1441 CCCTCCCTCC AGTAGATGTC TCGGCAAGTA TAGGAAGCTC CCCTGGGGTG GCCAGCAACC 
     1501 TGACAGAACC AAGTTACTCA AGTAGTACCT GTGGAAGCCA CACAGTACCT AGTCTTCATG 
     1561 CGGGGCTCCC GTCTCAGGAA TATGCTCCAG GATACAACGG ATCGTATTTG CATTCTACTT 
     1621 ACAGCAGCCA GCCCACACCT GCACTTCCTT CGCCTCATCC ATCCCCGTTG CATAGCTCTG 
     1681 GGCTCCTGCA GCCACCACCC CCTCCTCCTC CTCCACCCGC CCTGGTCCCA GGCTACAATG 
     1741 GGACTTCCAA TCTCTCCAGT TACAGCTATC CCTCTGCTAG CTATCCTCCT CAGACTGCCG 
     1801 TGGGGTCTGG GTACAGCCCT GGTGGAGCAC CCCCTCCTCC TTCCGCCTAC CTGCCTTCAG 
     1861 GAATTCCTGC GCCCACCCCT TTGCCCCCCA CTACTGTTCC TGGCTACACC TACCAAGGTC 
     1921 ATGGTTTGAC ACCCATCGCA CCCTCTGCTC TGACCAACAG TTCGGCAAGT TCTCTCAAAA 
     1981 GGAAAGCTTT CTATATGGCA GGGCAAGGAG ACATGGACTC CAGTTACGGA AATTACAGCT 
     2041 ATGGCCAACA GAGATCAACG CAGAGTCCTA TGTACCGAAT GCCCGACAAC AGCATTTCAA 
     2101 ACTCGAATCG GGGGAATGGC TTTGACAGAA ATGCTGAAAC ATCATCCTTA GCATTTAAGC 
     2161 CAACAAAGCA GCTGATGCCC TCTGAGCAGC AAAGGAAATT CAGCAGCCAG TCCAGTAGGG 
     2221 CTCTGACCCC TCCTTCCTAT AGTACTGCTA AAAATTCATT GGGATCAAGA TCGAGTGAAT 
     2281 CCTTTGGGAA GTATACGTCG CCAGTCATGA GTGAGCACGG GGACGACCAT CGGCAGCTCC 
     2341 TCGCTCATCC AATACAAGGT CCGGGACTCC GTGCAGCTAC CTCATCCAAC CACTCCGTGG 
     2401 ACGAGCAACT GAAGAATACT GACACGCACC TCATTGACCT GGTCACCAAT GAGATCATCA 
     2461 CCCAAGGACC TCCTGTGGAC TGGAGCGACA TAGCTGGTCT TGACCTGGTG AAGGCGGTTA 
     2521 TTAAGGAGGA GGTTTTGTGG CCAGTGTTGA GGTCAGATGC GTTCAGTGGG CTGACAGCCT 
     2581 TACCTAGGAG CATCCTTCTC TTTGGACCTC GGGGTACAGG CAAAACACTA TTGGGCAGAT 
     2641 GCATAGCCAG TCAGCTGGGG GCCACGTTTT TCAAAATTGC TGGTTCCGGA CTCGTTGCAA 
     2701 AGTGGCTAGG AGAAGCGGAG AAAATTATCC ACGCCTCTTT TCTTGTGGCC AGGTGTCGCC 
     2761 AGCCCTCGGT GATTTTTGTT AGTGACATTG ACATGCTTCT CTCCTCTCAA GTGAGTGAGG 
     2821 AACACAGTCC AGTGAGTCGG ATGAGAACCG AATTTCTGAT GCAGCTGGAC ACTGTACTGA 
     2881 CTTCGGCTGA GGACCAAATC GTAGTCATTT GTGCCACCAG TAAACCAGAA GAAATAGACG 
     2941 AATCTCTCCG GAGGTACTTC ATGAAAAGAC TTTTAATCCC ACTTCCTGAC AGCACAGCGA 
     3001 GGCACCAGAT AATAGTACAA CTGCTCTCAC AGCACAATTA CTGTCTCAAT GACAAGGAGT 
     3061 TTGCACTGCT CGTCCAGCGC ACAGAAGGCT TTTCTGGACT AGACGTGGCT CATTTGTGCC 
     3121 AGGAAGCAGC AGTGGGTCCT CTCCATGCTA TGCCAGCTAC AGACCTTTCA GCCATTATGC 
     3181 CCAGCCAGTT AAGGCCCATT ACATATCAAG ACTTTGAAAA TGCATTCTGC AAGATTCAGC 
     3241 CTAGCATATC TCAAAAAGAG CTTGATATGT ATGTTGAATG GAACAAAATG TTTGGTTGCA 
     3301 GTCAGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3361 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3421 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3481 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3541 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3601 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3661 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3721 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3781 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3841 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3901 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3961 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4021 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4081 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4141 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4981 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5041 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5101 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5161 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5221 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5281 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5341 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5401 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5461 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5521 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5581 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5641 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5701 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5761 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5821 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5881 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5941 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6001 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6061 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6121 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6181 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6601 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6661 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6721 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6781 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6841 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6901 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6961 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7021 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7081 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7141 AAATTTAACG CGAATTTT
//